- Type of paper
: Paper in journal
Title: Comparative studies of some substituted anilines as
altenative hydrogen donors in peroxidase reaction
- Authors:
- Reljić, Rajko
- Ries, Mihael
- Katušić, Silva
- Ries, Blanka (41020)
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 93
Year: 1991
Pages: from 27 to 30
Language: engleski
Summary: Three aniline derivatives, 2-aminohydroxybenzene,
2-amino-4-hydroxybenzene sulphonic acid, and
2-amino-4-chlorohydroxybenzene, have been assessed as sensitive hydrogen
donors for the spectrophotometric determination of hydrogen peroxide in the
presence of peroxydase. The absorbances of the coloured oxidation products
were significantly higher than that achieved with the
phenol/4-aminoantipyrine chromogenic system. Since these chromogens do not
require an additional substance for colour development, they are very
suitable for peroxidase kinetic studies.
Keywords: peroxidase reaction, substituted anilines-hydrogen donors
- Type of paper
: Paper in journal
Title: Principles and techniques of enzyme and cell
immobilization
- Authors:
- Mildner, Pavao (31322)
Journal: Prehrambeno-tehnološka i biotehnološka revija
Number: 1
ISSN: 0352-9193
Volume: 30
Year: 1992
Pages: from 17 to 24
Language: hrvatski
- Type of paper
: Paper in journal
Title: An improved color reagent for the serum urate
determination
- Authors:
- Reljić, Rajko
- Ries, Blanka (41020)
Journal: Acta Pharmaceutica
ISSN: 1330-0075
Volume: 42
Year: 1992
Pages: from 219 to 224
Language: engleski
Summary: An improved procedure for preparation of the final stage
reagent containing 3,5-dichloro-2-hydroxybenzenesulphonic
acid/4-aminoantipyrine chromogenic system, for enzymatic assay of urate in
human serum is described. The improvement was achieved in preparing a very
stable aqueous solution with the chromogen which was dispersed in micellar
form by the use of ethylene glycol and stabilized in HEPES buffer, pH 7.5.
Keywords: urate determination, enzymatic method
- Type of paper
: Paper in journal
Title: Cell wall of the yeast Saccharomyces cerevisiae
- Authors:
- Mrša, Vladimir (39525)
Journal: Prehrambeno-tehnološka i biotehnološka revija
Number: 1
ISSN: 0352-9193
Volume: 31
Year: 1993
Pages: from 43 to 50
Language: engleski
Summary: The aim of this review is to present a short survey of
information available on Saccharomyces cerevisiae cell wall, with a special
emphasis on its individual components, glucan, mannan, chitin, and cell
wall proteins.
Keywords: Saccharomyces cerevisiae cell wall, glucan, mannan, chitin, cell wall proteins
- Type of paper
: Paper in journal
Title: The requirement of N-glycosilation for efficient secretion
of yeast glycoproteins
- Authors:
- Mrša, Vladimir (39525)
- Reljić, Rajko
- Hughes, Roger Colin
- Barbarić, Slobodan (2072)
- Ries, Blanka (41020)
Journal: Acta Pharmaceutica
Number: 4
ISSN: 1330-0075
Volume: 44
Year: 1994
Pages: from 377 to 384
Language: engleski
Summary: The role of N-glycosylation in the secretion of
Saccharomyces cerevisiae proteins was studied. Model glycoenzymes acid
phosphatase and invertase both require glycosylation, since the inhibition
of this process by tunicamycin prevents the secretion of molecules
containing less than 50% carbohydrate chains. Enzyme forms with fewer
attached chains accumulated in the ER and were inactive. Besides,
N-glycosylation promoted the oligomerization of both invertase and acid
phosphatase dimers into native octameric structures. Acid phosphatase
expressed in baby hamster kidney cells was secreted in the active form with
fewer carbohydrate chains (2-3) than required in yeast, indicating a
difference in the protein folding in the ER and/or the secretion machinery
between yeast and animal cells.
Keywords: Glycosylation, secretion, yeast
- Type of paper
: Paper in journal
Title: Protein phosphatase activities of yeast acid and alkaline
phosphatases: comparison with authentic protein phosphatases
- Authors:
- Donella-Deana, Arianna
- Lopandić, Ksenija (86362)
- Ostojić, Sanja
- Pinna, Lorenzo A.
- Barbarić, Slobodan (2072)
Journal: Acta Pharmaceutica
Number: 4
ISSN: 1330-0075
Volume: 44
Year: 1994
Pages: from 367 to 376
Language: engleski
Summary: The role of yeast acid and alkaline phosphatases in protein
dephosphorylation was studied. Therefore, activity, specificity and kinetic
properties of nonspecific acid and alkaline phosphatase encoded by PHO5 and
PHO8 genes were examined. It was found that both enzymes efficiently
dephosphorylate some phosphoproteins and phosphopeptides, phosphoseryl
peptides being much better substrates than phosphothreonyl ones. Both
enzymes dephosphorylated also phosphotyrosyl peptides. On the basis of
their specificity, the two enzymes are distinctfrom both Ser/thr-specific
and phosphotyrosine protein phosphatases.
Keywords: Acid phosphatase, alkaline phosphatase, protein phosphatase, protein dephosphorylation, Saccharomyces cerevisiae
- Type of paper
: Paper in journal
Title: Molecular organization of the Saccharomyces cerevisiae
cell wall
- Authors:
- Vuković, Renata
- Hudina-Domladovec, Marijana
- Mrša, Vladimir (39525)
Journal: Prehrambeno-tehnološka i biotehnološka revija
Number: 2-3
ISSN: 0352-9193
Volume: 32
Year: 1994
Pages: from 99 to 102
Language: engleski
Summary: Interactions between different Saccharomyces cerevisiae
cell wall components were studied. It was found that wall proteins interact
with chitin in a way similar to what has already been reported for glucan.
Besides, glucan-glucan and glucan-chitin were observed, but no binding of
either proteins,glucan, or chitin to mannan chains of mannoproteins have
been detected. Results suggest a layered structure of the S. cerevisiae
cell wall with mannan chains forming the outer, and glucan building the
inner layer.
Keywords: Saccharomyces cerevisiae cell wall, glucan, mannan, chitin
- Type of paper
: Paper in journal
Title: Characterization of extracellular proteinases of the
fungus Acremonium chrysogenum 226-A
- Authors:
- Petković, Hrvoje
- Mrša, Vladimir (39525)
Journal: Prehrambeno-tehnološka i biotehnološka revija
Number: 2-3
ISSN: 0352-9193
Volume: 33
Year: 1995
Pages: from 91 to 95
Language: engleski
Summary: Proteolytic enzymes secreted by the fungus Acremonium
chrysogenum were characterized. Three proteinases were detected in the
culture broth, two enzymes were found to be serine proteinases, while the
third was a metalloproteinase. 95% of the proteolytic activity was due to
the two serine-type enzymes. Structural, as well as enzymatic properties of
the enzymes were studied. It was found that the proteinase pattern of A.
chrysogenum production strain 226-A clearly differs from that of other
strains of the same species.
Keywords: Proteinases - Acremonium chrysogenum
- Type of paper
: Paper in proceedings
Title: A process for stabilization of glycoproteins
- Authors:
- Česi, Vlasta (116565)
- Kozulić, Branko
- Barbarić, Slobodan (2072)
- Editors
- Buitelaar, R.M.
- Harder, A.
- van den Tweel, W.J.J.
Proceedings title: Stability and stabilization of enzymes
Language: engleski
Place: Maastricht, Nizozemska
Year: 1993
Pages: from 247 to 253
Meeting: Stability and Stabilization of Enzymes
Held: from 11/22/92 to 11/25/92
Summary: Using yeast invertase as a model glycoprotein,a novel
stabilization process consisting of periodate oxidation of susceptible
carbohydrates, followed by the reaction of generated aldehyde groups
withamino acid side chains, was developed. The amount and size of inter-
and intramolecularly cross-linked derivatives increased with the degree of
oxidation. Thermal stability of cross-linked derivatives also increased
with the degree of oxidation and reached a ten-times higher value than that
of the native enzyme.
Keywords: yeast invertase, enzyme stabilization, glycoproteins, enzyme cross-linking
- Type of paper
: Summary in proceedings
Title: Interactions of Saccharomyces cerevisiae extracellular
proteins with glucane
- Authors:
- Kobrehel, Irena
- Ugarković, Tatjana
- Mrša, Vladimir (39525)
- Editors
- Flogel, Mirna
Proceedings title: Godišnji sastanak hrvatskih biokemičara
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 74 to 74
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
- Type of paper
: Summary in proceedings
Title: Interactions of Saccharomyces cervisiae cell wall
components with chitin
- Authors:
- Hudina-Domladovec, Marijana
- Mrša, Vladimir (39525)
- Editors
- Flogel, Mirna
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB93
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 75 to 75
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
- Type of paper
: Summary in proceedings
Title: Staining of polyacrylamide electrophoresis gels for
exo-beta-1,3-glucanase activity
- Authors:
- Vargić, Tamara
- Mrša, Vladimir (39525)
- Editors
- Flogel, Mirna
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB93
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 82 to 82
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
- Type of paper
: Summary in proceedings
Title: A process for stabilization of glycoproteins
- Authors:
- Česi, Vlasta (116565)
- Kozulić, Branko
- Barbarić, Slobodan (2072)
- Editors
- Buitelaar, R.M.
- Harder, A.
- van den Tweel, W.J.J.
Proceedings title: Stability and Stabilization of Enzymes
Language: engleski
Place: Maastricht, Nizozemska
Year: 1992
Pages: from 0 to 0
Meeting: Stability and Stabilization of Enzymes
Held: from 11/22/92 to 11/25/92
- Type of paper
: Summary in proceedings
Title: Interactions of Saccharomyces cerevisiae extracellular
proteins with yeast glucane
- Authors:
- Mrša, Vladimir (39525)
- Ries, Blanka (41020)
- Ugarković, Tatjana
- Barbarić, Slobodan (2072)
- Editors
- Breitenbach, M.
- Schweyen, R.J.
- Pammer, M.
- Ruth, J.
Proceedings title: The sixteenth international conference on yeast genetics and molecular biology
Language: engleski
Place: Beč, Austrija
Year: 1992
Pages: from 513 to 513
Meeting: The sixteenth international conference on yeast genetics and molecular biology
Held: from 08/15/92 to 08/21/92
- Type of paper
: Summary in proceedings
Title: Mhetod for stabilization of glycoproteins
- Authors:
- Česi, Vlasta (116565)
- Kozulić, Branko
- Barbarić, Slobodan (2072)
Proceedings title: 22nd Meeting of the federation of European Biochemical Societies 93
Language: engleski
Place: Stockholm, Švedska
Year: 1993
Pages: from 224 to 224
Meeting: 22nd Meeting of the Federation of European Biochemical Societies
Held: from 07/04/93 to 07/09/93
- Type of paper
: Summary in proceedings
Title: Cabron source-regulated expression of the PHO5 gene
- Authors:
- Katušić, Silva
- Horz, Wolfram
- Barbarić, Slobodan (2072)
Proceedings title: XVII International Conference on Yeast Genetics and Molecular Biology
Language: engleski
Place: Lisabon, Portugal
Year: 1995
Pages: from 5351 to 5351
Meeting: XVII International conference on yeast genetics and molecular biology
Held: from 06/10/95 to 06/16/95
- Type of paper
: Summary in proceedings
Title: Purification and characterization of xylanase from
Thermomyces lanuginosus
- Authors:
- Cesar, Tatjana
- Mrša, Vladimir (39525)
- Editors
- Varljen, Jadranka
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB94
Language: engleski
Place: Rijeka
Year: 1994
Pages: from 25 to 25
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 10/14/94 to 10/15/94
- Type of paper
: Summary in proceedings
Title: Molecular organization of the Saccharomyces cerevisiae
cell wall
- Authors:
- Vuković, Renata
- Mrša, Vladimir (39525)
- Editors
- Varljen, Jadranka
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB94
Language: engleski
Place: Rijeka
Year: 1994
Pages: from 51 to 51
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 10/14/94 to 10/15/94
- Type of paper
: Summary in proceedings
Title: Expression of the acid phosphatase during yeast cell
growth on different carbon sources
- Authors:
- Katušić, Silva
- Kovarik, Zrinka
- Ries, Blanka (41020)
- Barbarić, Slobodan (2072)
- Editors
- Varljen, Jadranka
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB94
Language: engleski
Place: Rijeka
Year: 1994
Pages: from 73 to 73
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 10/14/94 to 10/15/94
- Type of paper
: Summary in proceedings
Title: Purification and properties of the Thermomyces lanuginosus
xylanase
- Authors:
- Cesar, Tatjana
- Mrša, Vladimir (39525)
- Editors
- Mildner, Boris
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB95
Language: engleski
Place: Zagreb
Year: 1995
Pages: from 61 to 61
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 09/22/95 to 09/25/95
- Type of paper
: Summary in proceedings
Title: Assembly of the yeast cell wall
- Authors:
- Vuković, Renata
- Jovičić, Mile
- Mrša, Vladimir (39525)
- Editors
- Mildner, Boris
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB95
Language: engleski
Place: Zagreb
Year: 1995
Pages: from 63 to 63
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 09/22/95 to 09/25/95
- Type of paper
: Prototype
Title: Stabilization of glycoproteins
- Authors:
- Kozulić, Branko
Institution of application: UK Patent Office
Date of application: 02/03/93
Registration number of application: GB 2 225 324B
Countries in which it is protected: Velika Britanija
Language: engleski
- Type of paper
: Prototype
Title: Stabilization of glycoproteins
- Authors:
- Kozulić, Branko
- Barbarić, Slobodan (2072)
Institution of application: United States Pattent Office
Date of application: 11/02/93
Registration number of application: 5,258,501
Countries in which it is protected: SAD
Language: engleski
- Type of paper
: M.A.
Title: Stabilization of glycoenzymes acid phosphatase and
invertase by cross-linking of their carbohydrate chains
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Author: ČESI VLASTA
Date of defense: 05/08/91
Language: hrvatski
Number of pages: 154
- Type of paper
: Mentorship
Title: Protein phosphatase activity of the Saccharomyces
cerevisiae alkaline phosphatase
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: BARBARIĆ SLOBODAN
Date of defense: 06/25/91
Number of pages: 137
Author: Ostojić Sanja
Degree level: M.A.
- Type of paper
: Mentorship
Title: Glycosylation of yeast acid phosphatase expressed in
animal cells
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: RIES BLANKA
Date of defense: 10/21/92
Number of pages: 95
Author: Reljić Rajko
Degree level: M.A.
- Type of paper
: Mentorship
Title: Purification and characterization of xylanase from the
fungus Thermomyces lanuginosus
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: MRŠA VLADIMIR
Date of defense: 07/07/95
Number of pages: 103
Author: Cesar Tatjana
Degree level: M.A.
- Type of paper
: Mentorship
Title: Biosyntesis, partial purification and characterization of
proteolytic enzymes of the fungus Acremonium chrysogenum 226-A
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: MRŠA VLADIMIR
Date of defense: 12/20/94
Number of pages: 92
Author: Petković Hrvoje
Degree level: M.A.
- Type of paper
: Mentorship
Title: The regulation of expression of the gene PHO5 during the
growth of yeast cells on different carbon sources
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: BARBARIĆ SLOBODAN
Date of defense: 01/31/95
Number of pages: 100
Author: Katušić Silva
Degree level: M.A.
- Type of paper
: Mentorship
Title: Interactions of proteins with the yeast Saccharomyces
cerevisiae glucan
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: MRŠA VLADIMIR
Date of defense: 06/25/92
Number of pages: 58
Author: Ugarković Tatjana
Degree level: D.A.
- Type of paper
: Mentorship
Title: Influence of phosphate on the synthesis of
tiamine-repressible acid phosphatase of the yeast Saccharomyces cerevisiae
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: RIES BLANKA
Date of defense: 10/25/91
Number of pages: 52
Author: Katušić Silva
Degree level: D.A.
- Type of paper
: Mentorship
Title: Localization of exo-beta-1,3-glucanase of the yeast
Saccharomyces cerevisae
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: MRŠA VLADIMIR
Date of defense: 02/25/93
Number of pages: 60
Author: Kobrehel Irena
Degree level: D.A.
- Type of paper
: Mentorship
Title: Interactions of Saccharomyces cerevisiae cell wall
components with chitin
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: MRŠA VLADIMIR
Date of defense: 05/25/93
Number of pages: 58
Author: Hudina-Domladovec Marijana
Degree level: D.A.
- Type of paper
: Mentorship
Title: Isolation of pNPP specific alkaline phosphatase from the
yeast Saccharomyces cerevisiae
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: BARBARIĆ SLOBODAN
Date of defense: 05/25/94
Number of pages: 39
Author: Mutić Monika
Degree level: D.A.
- Type of paper
: Mentorship
Title: Intermolecular interactions of the Saccharomyces
cerevisiae cell wall components
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: MRŠA VLADIMIR
Date of defense: 06/25/94
Number of pages: 48
Author: Vuković Renata
Degree level: D.A.
- Type of paper
: Mentorship
Title: The influence of proteolysis on the Saccharomyces
cerevisiae cell wall porosity
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: MRŠA VLADIMIR
Date of defense: 09/29/95
Number of pages: 53
Author: Jovičić Mile
Degree level: D.A.
- Type of paper
: Invited lecture
Title: Protein phosphatase avtivities of yeast acid and alkaline
phosphatases: comparison to authentic protein phosphatases
Institution: kongres
Year: 1993
- Type of paper
: Invited lecture
Title: The requirement of N-glycosylation for the efficient
secretion of yeast glycoproteins
Institution: kongres
Year: 1993
- Type of paper
: Invited lecture
Title: Structure of the Saccharomyces cerevisiae cell wall
Institution: kongres
Year: 1994
- Type of paper
: Invited lecture
Title: Interactions of the transcriptional activators Pho4 and
Pho2 in the regulation of a phosphatase gene family in yeast
Institution: kongres
Year: 1995