SVIBOR - Papers quoted in CC - project code: 1-07-073
MINISTRY OF SCIENCE AND TECHNOLOGY
Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr
SVIBOR - Collecting Data on Projects in Croatia
Papers quoted in Current Contents on project 1-07-073
Quoted papers: 3
Other papers: 7
Total: 10
Title: Labeling of Precursor Pools for Glycosphingolipid
Biosynthesis. Incorporation of 3H Galactose by Rat Hepatocytes in Primary
Culture
- Authors:
- Mesari, Marko (30466)
- Decker, Karl
Journal: Biological Chemistry Hoppe-Seyler
Number: 11
ISSN: 0177-3593
Volume: 371
Year: 1990
Pages: from 1051 to 1057
Number of references: 9
Language: engleski
Summary: UDP-galactose and UDP-glucose are the immediate sources of
monosaccharide residues in glycosphingolipid biosynthesis. The
incorporation of 6-3H-galactose into these comounds was measured in primary
cultures of rat hepatocytes, which take up and metabolize galactose
rapidly. The UDP-glucose and UDP-galactose content of hepatocytes,
determined enzymatically and by the HPLC analysis of UDP-sugars, was
1.870.22 and 0.510.06 nmol/mg protein, respectively. Galactose
concentrations in the medium of up to 7.5 M did not influence the
intracellular levels of UDP-glucose and UDP-galactose. Although the
specific radioactivity of these precursor pools did not reach constant
plateau, conditions were defined that allow calculation of rates of
glycolipid synthesis from added labeled galactose. They include the
replacement of glucose in the culture medium by sodium pyruvate and
D-galactose.
Keywords: Precursor pool, specific activity, UDP-galactose, UDP-glucose
Title: Sialyltransferase Activities in Cultured Rat Hepatocytes
- Authors:
- Mesari, Marko (30466)
- Decker, Karl
Journal: Biochemical and Biophysical Research Communications
Number: 1
ISSN: 0006-291X
Volume: 171
Year: 1990
Pages: from 132 to 137
Number of references: 30
Language: engleski
Summary: Previous studies on the age and sex dependency of the
ganglioside patterns in rat liver in vivo and the concomitant determination
of the activities of some enzymes involved in these pathways revealed the
prominent role of the sialylation of GM3 to GD3 in determining the flow to
the mono "a"- and polysialo "b"-series, respectively. Here, the influence
of hormones on the activities of GM3 and GD3 synthases in isolated
hepatocytes was studied. The combination of several factors (insulin,
glucagon, EGF, glucocorticoids) was found to be necessary for maintaining
in vivo activity levels of GD3- but not of GM3-synthase.
Keywords: GM3 synthase, GD3 synthase, primary culture, rat hepatocytes
Title: Activation of GD3 Synthase by Sex Steroid Hormones in
Cultured Rat Hepatocytes
- Authors:
- Mesari, Marko (30466)
- Decker, Karl
Journal: Biochemical and Biophysical Research Communications
Number: 3
ISSN: 0006-291X
Volume: 171
Year: 1990
Pages: from 1188 to 1191
Number of references: 16
Language: engleski
Summary: The influence of sex steroid hormones on the activity of
GM3 and GD3 synthases in isolated hepatocytes was studied. Progesterone
(0.1 - 20 M), -estradiol (0.1 - 1 M), and testosterone (0.1 -1 M)
activate GD3 but not GM3 synthase when added directly to hepatocytes
cultured in modified William's E medium.
Keywords: GM3 synthase, GD3 synthase, primary culture, rat hepatocytes, testosterone, progesterone. -estradiol
Information: svibor@znanost.hr
