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Papers quoted in Current Contents on project 1-07-102

Quoted papers: 25
Other papers: 137
Total: 162

Title: Age dependent changes of intracellular immunity against virus infections

Authors:: Schr”der, H. C.; Kelve, M.; Pfeifer, K.; Flogel-Mršiæ, Mirna (56805); Mller, W.E.G.

Journal: Zeitschrift fur Gerontologie
ISSN: 0044-281x
Volume: 26
Year: 1993
Pages: from 232 to 237
Language: njemaèki
Summary: A study of the antiviral 2',5'-oligoadenylate(2-5A) system indifferent tissues of rats of different age (new-born: 1 day old;young adult: 2-3 months old; middle aged adults: 12-month old;old: 32-33 month old) revealed that the activities of the 2-5Ametabolic enzymes alter during aging and development. Wedemonstrate that soluble 2-5A synthetase (2-5OAS) activitystrongly increases after birth, reaching maximal levels in youngadult and middle-aged adult animals and then significantlydecreases with age. In contrast, the activity of 2',3'exoribonuclease which inactivates 2-5A increases by three-foldwith age. The decrease in 2-5OAS activity and increase in 2-5Anuclease activity were found to result in a decrease in thecellular 2-5A content with age...
Keywords: 2'5'-oligoadenylate synthetase, RNase L, ageing - antiviral defence

Title: HnRP CBP35-CBP67 interaction during stress response and ageing

Authors:: Lauc, Gordan (201852); Seve, A.P.; Hubert, J.; Flogel-Mršiæ, Mirna (56805); Schr”der, H. C.; Mller, W.E.G.

Journal: Mechanisms of Ageing and Development
ISSN: 0047-6374
Volume: 70
Year: 1993
Pages: from 227 to 237
Number of references: 42
Language: engleski
Summary: Previous studies demonstrated the existence of nuclear carbohydrate binding proteins in a variety of mammalian cells with molecular masses of 35,000, 67,000, and 70,000 (CBP35, CBP67, and CBP70), which are associated with nuclear ribonucleoprotein (RNP) complexes. CBP35 consists of two domains, an amino-terminal portion that is homologous to certain regions of proteins of the heterogeneous nuclear RNP complex and a carboxyl-terminal portion that is homologous to ß-galactoside-specific lectins, and has been proposed, likewise the glucose-specific lectin, CBP67, to guide RNP complexes through the nuclear pore. Here we show that exposition of mature rats to stress induces an increase in nuclear CBP35 bound to CBP67 and retained on immobilized glucose. Nuclear extracts from the livers of old rats displayed no detectable stress response. This CBP35_CBP67 association detected in rat liver is considered with respect to the CBP35_CBP70 association recently observed in HL60 cell nuclear extracts.
Keywords: HnRNP, nuclear lectin, transport, CBP35, CBP67, CBP35 and CBP67 in stress, rat liver

Title: Entropies of coding and noncoding sequences of DNA and proteins

Authors:: Lauc, Gordan (201852); Iliæ, Igor; Heffer-Lauc, Marija

Journal: Biophysical Chemistry
ISSN: 0301-4622
Volume: 42
Year: 1992
Pages: from 7 to 11
Number of references: 45
Language: engleski
Summary: The entropies of protein coding genes from E. coli were calculated according to Boltzmann's formula. With nucleotides as code units, the entropies of the coding regions, when compared to the entropies of complete sequences (leader and coding region as well as trailer) were seen to be lower but with a marginal statistical significance. With triplets of nucleotides as code units, the entropies of correct reading frames were significantly lower than the entropies of frameshifts +1 and -1. With amino acids as code units, the results were opposite: Biologically functional proteins had significantly higher entropies than proteins translated from the frameshifted sequences. We attempt to explain this paradox with the hypothesis that the genetic code may have the ability of lowering information content (increasing entropy) of proteins while translating them from DNA. This ability might be beneficial to bacteria because it would make the functional proteins more probable (having a higher entropy) than nonfunctional proteins translated from frameshifted sequences.
Keywords: entropy, information content, genetic code, frameshift mutation

Title: Arginase, a new marker of mammary carcinoma

Authors:: Štraus, Božidar; Èepelak, Ivana (7950); Festa, Galib

Journal: Clinica Chimica Acta
ISSN: 0009-8981
Volume: 210
Year: 1992
Pages: from 5 to 12
Number of references: 17
Language: engleski
Summary: Activities of arginase, ALT, AST, and ALP were determined in seraof healthy women, women with verified carcinoma of breast, benignmasthopathy and a group of patients with carcinoma of variousorgans and of patients with acute viral hepatitis. Serum arginaseactivity is raised only in mammary cancer when breast disease wasanalyzed and in both benign and malignant liver disease. Howeverratios ALT/arginase, AST/arginase and ALP/arginase differsignificantly. The use of these quotients implies a high degreeof confidence in differentiating mammary carcinoma fromhepatitis.
Keywords: arginase-mammary cancer-diagnostics, hepatitis-diagnostics, mammary cancer-diagnostics, ALP, AST, ALT

Title: Rapid and simple method for determination of copper, manganese and zinc in rat liver by direct flame atomic absorption spectrometry

Authors:: Luterotti, Svjetlana (26936); Žaniæ-Grubišiæ, Tihana (80883); Juretiæ, Dubravka (19264)

Journal: Analyst
ISSN: 0003-2654
Volume: 117
Year: 1992
Pages: from 141 to 143
Number of references: 18
Language: engleski
Summary: An acidic homogenate method and direct nebulization flame atomicabsorption spectrometry is successfully applied to thesimultaneous determination of Cu, Mn, and Zn in rat liver. Onlytwo steps in sample pretreatment are needed. 101% for Cu, 98% forMn, and 100% for Zn recovery could be achieved using aqueousstandards. Limits of detection in bilogical media were 0.04,0.03, and 0.04 mg/l for Cu, Mn, and Zn respectively.
Keywords: atomic absorption - direct flame, Cu, Mn, Zn

Title: Urinary glycosaminoglycans in different phases of Balkan endemic nephropathy

Authors:: Juretiæ, Dubravka (19264); Èvorišèec, Dubravka; Holjevac-Vukoviæ, A.; Benkoviæ, J.; Lipovac, Krešo (25961); Stavljeniæ, Ana

Journal: Nephron
ISSN: 0028-2766
Volume: 65
Year: 1993
Pages: from 564 to 567
Number of references: 19
Language: engleski
Keywords: glycosaminoglycans, chondroitin sulphate, heparan sulphate, beta-2 microglobuline

Title: Reply to Wada's comments on the entropies of coding and noncoding sequences of DNA and proteins

Authors:: Lauc, Gordan (201852); Heffer-Lauc, Marija

Journal: Biophysical Chemistry
ISSN: 0301-4622
Volume: 44
Year: 1992
Pages: from 79 to 80
Number of references: 2
Language: engleski
Keywords: enthropy of coding DNA sequence, enthropy of noncoding DNA sequence, enthropy of protein sequence

Title: Human brain gangliosides in development, aging and disease

Authors:: Kraèun, I.; Rosner, A.; Drnovšek, V.; Heffer-Lauc, Marija; Æosoviæ, È.; Lauc, Gordan (201852)

Journal: International Journal of Developmental Biology
ISSN: 0012-1606
Volume: 35
Year: 1991
Pages: from 289 to 295
Number of references: 38
Language: engleski
Summary: Brain gangliosides in prenatal and postnatal human life andAlzheimer's disease were analysed. Composition of gangliosidesduring ageing follow region specific alterations: In the frontalcortex GD1n GM1 decrease more then GT1b and GD1b. There is noindividually characteristic change in the visual cortex. Inhyppocampus GD1a is decreased while other fractions are stable.In cerebellar cortex GD1b and GT1b fractions decrease withageing. In Alzheimer's disease all gangliosides are stronglydecreased. GN2 and GN3 are elevated indicating lysosomaldegradation of gangliosides.
Keywords: gangliosides, human brain, development, ageing, Alzheimer'sdisease

Title: cDNA sequence of cyclophilin from Dictyostelium discoideum

Authors:: Barišiæ, Karmela (187331); Mollner, S.; Noegell, A.A.; Gerisch, G.

Journal: Developmental Genetics
ISSN: 0192-253
Volume: 12
Year: 1991
Pages: from 50 to 53
Number of references: 18
Language: engleski
Summary: A cDNA encoding a protein homologous to cyclophilins from otherspecies has been isolated from Dictyostelium discoideum cDNAlibrary. From deduced amino acid sequence protein with a Mr od 19kD and 64% identity with human cyclophilin is predicted. Southernblott analysis indicates that there is one cyclophiline gene inthe D.discoideum genome. The mRNA is present in all developmentalstages.
Keywords: Dictyostelium, protein folding, peptidyl-prolyl isomerase

Title: A nonradioactive sensitive method for the detection of DNA fragmentation in apoptotic cells

Authors:: Lauc, Gordan (201852); Peroviæ, S.; Dapper, J.; Flogel-Mršiæ, Mirna (56805); Mller, W.E.G.; Iskriš, Sonja

Journal: Analytical Cellular Pathology
ISSN: 0921-8912
Volume: 7
Year: 1993
Pages: from 104 to 114
Number of references: 24
Language: engleski
Summary: A new method is described which is suitable to analyze reliably small amounts of DNA for the existence of apoptotic fragmentation. After isolation, DNA is labelled with biotin-4-dUTP using Klenow polymerase. Then DNA is size-separated by agarose gel electrophoresis followed by blot transfer and subsequently visualized by the streptavidin alkaline phosphatase-BCIP/NBT procedure. This nonradioactive method was used to detect apoptotic DNA in rat pheochromocytoma PC12 cells, treated with tributyltin (3 ng/ml). While only 30 ng of DNA is required for analysis of apoptotic DNA using the new blot technique, 100-fold more materials is needed to identify the fragmentation of DNA after separation by agarose gel electrophoresis and direct staining with ethidium bromide. In a further set of experiments rat cortical cells are incubated with human immunodeficiency virus type 1 viral glycoprotein of Mr of 120 kDa (gp120) to induce apoptosis. Applying the direct procedure > 0.3 ng of gp120/ml are required to analyze apoptotic DNA; only 0.1 ng gp120/ml or less are sufficient to document clear DNA fragmentation using the nonradioactive blotting technique described here. These results demonstrate that the new procedure is applicable to analyze small amounts of apoptotic DNA and shows that the apoptosis-inducing effect of gp120 can be measured at low concentrations.
Keywords: apoptosis-detection of, biotin-labeling of DNA, HIV-1, GP120, DNA-fragmentation, cortical cells

Title: Identification and purification of a stress associated nuclear carbohydrate binding protein (Mr 33000) from rat liver by application of a new photoreactive carbohydrate probe

Authors:: Lauc, Gordan (201852); Flogel-Mršiæ, Mirna (56805); Diehl-Seifert, Barbel; Schr”der, H. C.; Mller, W.E.G.

Journal: Glycoconjugate Journal
ISSN: 0282-0080
Volume: 11
Year: 1994
Pages: from 541 to 549
Number of references: 39
Language: engleski
Summary: A photoreactive a-D-glucose probe has been designed for the specific detection of carbohydrate binding proteins (CBPs). The probe consists of four parts: (i) an a-D-glucose moiety, (ii) the digoxigenin tag, (iii) the photoreactive cross-linker and (iv) the lysil-lysine backbone. After incubation with lectins in the dark, the probe is activated and cross-linked to the CBPs after being treated by several flashes. Using this method we have identified a new a-D-glucose CBP of Mr = 33,000, termed CBP33, in the nuclei of rats exposed to transient immobilization stress. Monoclonal antibodies were raised against the partially purified protein and subsequently used to enrich CBP33. It was purified (> 2,400-fold) to apparent homogeneity from a 0.6 M nuclear salt extract by two subsequent affinity chromatography steps (antibody-affinity as well as a-D-glucose affinity column).
Keywords: carbohydrate binding proteins, lectins, stress, rat liver, CBP33

Title: Glycoprotein and ganglioside changes in human trophoblasts after exposure to pulsed Doppler ultrasound

Authors:: Heffer-Lauc, Marija; Latin, V.; Breyer, B.; Flogel-Mršiæ, Mirna (56805); Mller, W.E.G.; Lauc, Gordan (201852)

Journal: Ultrasound in Medicine and Biology
Number: 4
ISSN: 0301-5629
Volume: 21
Year: 1995
Pages: from 579 to 584
Number of references: 40
Language: engleski
Summary: Changes in glycoprotein and ganglioside composition in human trophoblasts (8th week of gestation) after in vitro exposure to pulsed Doppler ultrasound (pulse duration 1.22 ms; repetition frequency 11.1 KHz; center frequency 4 MHz; ISPPA=175.5 W/cm2; ISPTA=0.59 W/cm2) were investigated. Evacuated trophoblasts were divided in two halves and insonated for 10 min on top of a 6 cm layer of 5% gelatin in 50 ml tubes (Falcon) at 37°C. One half of each trophoblast was sham insonated and served as an internal control. After insonation trophoblasts were maintained at 37°C for 24 h. Glycoproteins were detected using a-D-mannose specific lectins from Galanthus nivalis and Narcissus pseudonarcissus. A decrease in the expression of mannose containing glycoprotein mgp47 and an increase in expression of mgp54 were observed. Ganglioside composition was also significantly altered. Concentrations of two gangliosides migrating similarly to GM2, and one similarly to GQ1, decreased for more than 75%. At the same time concentrations of one ganglioside migrating similarly to GM3, and two other unidentified gangliosides increased two- to fourfold.
Keywords: Glycoproteins, Gangliosides, Lectins, Pulsed Doppler ultrasound, Safety

Title: CBP35/CBP67 interaction during stres response and ageing

Authors:: Schr”der, H. C.; Lauc, Gordan (201852); Seve, A.P.; Hubert, J.; Flogel-Mršiæ, Mirna (56805); Mller, W.E.G.

Journal: Zaitschrift fur Gerontologie
ISSN: 0044-281x
Volume: 27
Year: 1994
Pages: from 200 to 203
Number of references: 28
Language: njemaèki

Title: Sperm motility and kinetics of dynein ATPase in astheno- and normozoospermic samples after stimulation with adenosine and its analogues

Authors:: Romac, Pavle; Žaniæ-Grubišiæ, Tihana (80883); Èuliæ, Ognjen (137063); Cvitkoviæ, M; Flogel-Mršiæ, Mirna (56805)

Journal: Human Reproduction
ISSN: 0268-1161
Volume: 9
Year: 1994
Pages: from 1474 to 1478
Language: engleski

Title: Biotinylated carbohydrate markers - A novel tool for lectin research

Authors:: Lauc, Gordan (201852); Flogel-Mršiæ, Mirna (56805); Mller, W.E.G.

Journal: Zeitschrift fur Naturforshung
ISSN: 0939-5075
Volume: 49c
Year: 1994
Pages: from 843 to 848
Number of references: 18
Language: engleski
Summary: One of the key impediments in the research of lectins is the lack of specific techniques for their detection. Novel markers, biotin-labeled carbohydrates, could help to overcome this problem. Being at least 10 times more sensitive than neoglycoproteins in the membrane screening assays, they also enable direct detection of lectins in complex mixtures. The markers were synthesized by linking biotin to one, and a carbohydrate (galactose or glucose) to the other amino group of (the amino acid) lysine. After synthesis the markers were chromatographically purified on a lectin (RCA for galactose-marker, ConA for glucose-marker) and avidin affinity columns. The applicability of the markers to detect lectins was demonstrated e.g. on sponge extracts (from Geodia cydonium). Following the incubation of biotin labeled carbohydrates covalent crosslinking between lectins and markers was induced by UV radiation. After transferring to the blotting membrane, lectins were detected with deglycosylated anti-biotin antibody labeled with alkaline phosphatase. Beside the crosslinking technique, the biotinylated carbohydrate markers were also used for detection of lectins on the nitrocellulose membrane in gene library screening and slot-blotting.
Keywords: lectins, carbohydrate-binding proteins, method

Title: Effects of sodium valproate on renal cell brus-border enzymes in rats

Authors:: Èepelak, Ivana (7950); Rekiæ, Branka; Juretiæ, Dubravka (19264); Mandušiæ, Anka

Journal: European Journal of Clinical Chemistry and Clinical Biochemistry
ISSN: 0939-4974
Year: 1995
Number of references: 29
Language: engleski
Summary: The effect of sodium valproate (200 mg/kg body weight) on renal cells was investigated during a 10-day trial, by determining the catalytic activities of alanine aminopeptidase, Y-glutamysferase and alkaline phosphatase in the membrane cell brush-border of the renal proximal tubules. Four days after the administration of sodium valproate, a significant increase was observed in the volume of urine(9.2_4.2 ml/18-h volume; control group, 3.06_1.8 ml/19-h volume), and in the catalytic activites of Y- glutamyltransferase (2.69 times that measured in the control group) and alkaline phosphatase (3.02 times thatmeasured in the control group) in urine. After prolonged in the control group) in urine. After prolonged treatment the urine became alkaline (pH=10.5 on day 10), thereby excluding alanine aminopeptidase as a useful indicator of renal cell changes following the administration of sodium valproate. The activities of alkaline phosphatase and Y-glutamyltransferase in isolated vesicles of renal brush-border membrane cells were significantly increased (p<0.05) and decreased, respectively. On the basis of the results obtained, we believe that the determination of the catalytic activities of Y- glutamyltransferase and alkaline phosphatase in urine might prove useful for the follow-up of the renal cell state during therapy with sodium valproate.

Title: Ochratoxin A impairs activity of the membrane bound enzymes in rat pancreas

Authors:: Žaniæ-Grubišiæ, Tihana (80883); Zrinski, Renata (188343); Èepelak, Ivana (7950); Petrik, Jozsef (180761); Pepeljnjak, Stjepan (36114)

Journal: European Journal of Clinical Chemistry and Clinical Biochemistry
ISSN: 0939-4974
Volume: 33
Year: 1995
Number of references: 42
Language: engleski
Summary: Ochratoxin A is a mycotoxin produced by Aspergillus ochraceus and is a natural contaminant of moldy food. Ochratoxin A has a number of toxic effects, some of which may be related to the changes in the cell membrane. We measured the activities of 5 pancreatic, membrane bound enzymes in female Fisher rats that were given low doses of ochratoxin A (120 ug/kg body weight per day) during 20-35 days. The amount of toxin corresponds to 1.5 mg/kg in the feed, daily. These doses are in the range of natural contamination found in feed. The enzymes studied were alanine aminopeptidase, alkaline phosphatase, ecto-Ca2+/Mg2+- ATPase, Y-glutamyltransferase and ecto-5,-nucleotidase. Tretment lasting 20 days caused a strong decrease in the activity of alanine aminopeptidase, Ca2+/Mg2+-ATPase and alkaline phosphatase to 0.76-0.04, 0.53_0.03 and 0.30 _0.02 of the control values, respectively (p<0.05). No significant changes in the activity of Y-glutamil transferase and 5,- nucleotidase were observed. However, activity of alanine aminopeptidase returned to normal values after 35 days of treatment, suggesting an adaptation of the organism, or a substitution of a released enzyme. Activities of alkaline phosphatase and Ca2+/Mg2+-ATPase remained significantly reduced to 0.42_0.03 and 0.52_0.04. respectively (p<0.01). We conclude that treatment of rats with low doses of ochratoxin A resulted in reduction of the activities of the membrane bound enzymes, most probably by inducing their release, as a result of the impairment of the functional integrity of cell membranes.
Keywords: Ochratoxin A, Rat pancreas, Membrane bound enzymes

Title: Follicular fluid contents of hyaluronic acid, follicle-stimulating hormone and steroids relative to the success of in vitro fertilization of human oocytes

Authors:: Suchanek, E.; Šimuniæ, V; Juretiæ, Dubravka (19264); Grizelj, V.

Journal: Fertility and Sterility
ISSN: 0015-0282
Volume: 62
Year: 1994
Pages: from 347-3 to 52
Language: engleski

Title: Ecto-ADPase in the rat renal brush-border membranes

Authors:: Žaniæ-Grubišiæ, Tihana (80883); Zrinski, Renata (188343); Gripariæ, Lorena; Flogel-Mršiæ, Mirna (56805)

Journal: Croatica Chemica Acta
ISSN: 0011-1643
Year: 1995
Language: engleski
Summary: Evidence is presented that brush-border membrane vesicles purified from homogenates of rat kidney cortex posses an ecto-enzyme activity that is responsible for the hydrolysis of both ATP and ADP, as well as for the hydrolysis of other nucleoside tri- and diphosphates. The enzyme could not be classified as ADP diphosphohydrolase (EC 3.6.1.5) or as ATPase (EC 3.6.1.3) for it is sharing some characteristics with both enzymes. The ADP and ATP hydrolyzing activities both have broad pH optimum, between 7.2 and 8.6. In the presence of Ca2+ ions ADP hydrolysis is following the simple Michaelis-Menten kinetics assuming a single catalytic site. However, in the absence of the divalent cations the activity was reduced to a insignificant levels. Results suggest that the true substrate for ADPase is a divalent cation conjugated ADP. It was found that Ca2+ was more stimulating than Mg2+.Denaturation experiments, that involved treatment of vesicles with trypsin and papain , as well as heat denaturation curves, indicated that only one enzyme was involved in the hydrolysis of nucleoside di- and triphosphates. ADP and ATP hydrolysis was found to be resistant to limited proteolysis, while detergents induced strong inhibition. The inhibitors of other known ATPases i.e. ouabain, levamisole, oligomycin, vanadate and concanavalin A, respectively, did not affect the rate of hydrolysis. However, hydrolysis of both ADP and ATP was inhibited by: diethyl pyrocarbonate, dithiothreitol, high concentrations of both N-ethylmaleimide and azide, indicating that histidine and sulphydrile groups may be essential for the catalytic reaction. The inhibition produced by diethyl pyrocarbonate was reversed by subsequent treatment with hydroxylamine supporting importance of histidine and/or tyrosine residues for the ecto-ADPase and ecto-ATPase activities. Based on the results presented it is proposed that both ADPase and ATPase activities reside within the same protein which is capable to hydrolyze extracellular ATP to AMP yielding eventually adenosine in the presence of 5'nucleotidase


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