SVIBOR - Papers quoted in CC - project code: 1-08-089

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Papers quoted in Current Contents on project 1-08-089

Quoted papers: 10
Other papers: 32
Total: 42

Title:

Authors:: Lucu, Čedomil (26622)

Journal: Comp. Biochem. Physiol.
Number: 3
ISSN: 0300-9629
Volume: 97
Year: 1990
Pages: from 297 to 306
Number of references: 93
Language: engleski
Summary: This review paper is focused on ion permeability, the action of drugs on ion transport, the aspects which are relevant to theionic regulatory mechanisms of isolated gill epithelia or their subcellular fractions in aquatic Brachyuran Crustacea.Prerequisite for the transition of brachyuran Crustacea from the sea to a brackish or freshwater environment is homeostasis of the blood concentration, attained by the gill selective ion absorption mechanisms.The effects of the activities of the enzymes Na,K ATPase and carbonic anhydrase on the transport mechanisms were discussed.In the isolated gill preparation Na and Cl absorptive transport mechanisms were reported.Further adaptive characteristics found preferentially in the brachyuran posterior gills in diluted medium are increased energy processes (oxydation role of glucose,amino acids,mitochondria oxydative enzymes,oxygen consumption). Furthermore,the activities of the enzymes Na,K ATPase and carbonic anhydrase (CA) which are involved directly or indirectly in the ionic transport mechanisms are increased.Under the described conditions,Na,K ATPase is basolaterally localized and oriented to discharge Na from the cytosol across the basolateral membrane side into the hemolymph and actively absorb K into the cells.It seems to be the case that adaptive strategies of the increased gill Na,K ATPase activity under low salinity stress occurs by the (1) short term acclimation and by means of biogenic amine induction of the existing enzyme and (2) by long term synthesis of the enzyme "de novo" and an increase of the number of chloride cells.
Keywords: Ionic regulation, Crustacea, gill epithelium, permeability,transbranchial potential, Na/H exchanger, Cl/HCO3 exchanger,enzymes Na,K ATPase and carbonic anhydrase.

Title:

Authors:: Lucu, Čedomil (26622); Siebers, Dietrich; Nenishiene, Viligaile; Skaramuca, Boško; Spaargaren, Dirk

Journal: Comp. Biochem. Physiol.
ISSN: 0300-9629
Volume: 102
Year: 1992
Pages: from 307 to 310
Number of references: 25
Language: engleski
Summary: Rubidium transport and inorganic osmolyte permeabilities were studied in isolated perfused Carcinus gills. The gill permeability coefficient calculated from the unidirectional ion fluxes in the perfused gills in dilluted seawater identical to the bath showed more cation than anion selectivity, showing the following permeability ratios P-Rb:P-Na:P-Cl=1.7:1.0:0.2. Net Rb secretive fluxes from the hemolymph across the basolateral and apical membrane surfaces into the bathing solution werei nhibited (51%) by basolaterally applied ouabain (5 x 10-3 moll-1). The results suggest the presence of ouabain-sensitive active Rb secretion where the mechanisms are basolaterally located. Net rubidium fluxes were not changed by externally applied amiloride.
Keywords: Rubidium, isolated gill epithelium, fluxes,permeability ratios.

Title:

Authors:: Siebers, Dietrich; Hentschel, Jan; Bottcher, Kirstin; Lucu, Čedomil (26622)

Journal: Helgolander Meeresunters.
ISSN: 0174-3597
Volume: 46
Year: 1992
Pages: from 435 to 445
Number of references: 22
Language: engleski
Summary: Posterior gills of shore crab Carcinus maenas were homogenizedand fractionated by means of differential and density gradientcentrifugation. Employment of marker enzymes Na-K-ATPase andcarbonic anhydrase for plasma membranes and cytochrome oxydasefor mitochondria showed that these structural elements wereseparated.An ATPase which did not depend on the presence ofsodium (20 mM) ions in the incubation medium but of the presenceof potassium (20 mM) ions only was found in mitochondrialfractions.The mitochondrial ATPase was tightly bound to cellularparticulates and activate approximately threefold by bicarbonate(20 mM) ions.Mitochondrial ATPase is suitable marker formitochondria.The results obtained confirm literature data on highmitochondrial densities in crab gills and allow the consumptionof significant rates of energy metabolism in these organs. The mitochondrial ATPase activity was nearly completely inhibited by oligomycin (1microg/ml) and gratly inhibited by low levels of thyocianate and calcium ions, the Ki for Ca being 4mM.Ultramicroscopic investigations of combined fractionsconfirmed the presence of the respective mitochondrial andvesicular plasma membrane structures. Considering its properties the mitochondrial ATPase is clearlydistinct from crab gill Na-K-ATPase and can be measuredspecifically in samples containing Na,K ATPase. MitochondrialATPase is therefore considered a suitable and reliable marker enzyme for mitochondria.
Keywords: Mitochondrial ATPase, Carcinus, posterior gills,differential and density gradient centrifugation,oligomycin,marker enzyme.

Title:

Authors:: Lucu, Čedomil (26622)

Journal: J. Exp. Zool.
ISSN: 0022-104
Volume: 265
Year: 1993
Pages: from 378 to 386
Number of references: 53
Language: engleski
Summary: In recent years a convincing body of knowledge has been accumulated on ionic concentrations in the organisms but less indicating transport mechanisms for osmolytes in marine and brackish water Crustacea. Cuticle and epithelium cells serve as selective communication for the exchange of ions and small organic molecules between crustacean gills and their environment.In addition to the presence of the ouabain sensitive unidirectional Na exchange located on the basolateral gill surface, there is also located an oppositely-directed, ouabain sensitive Rb(K) unidirectional flux.Basolaterally applied ouabain inhibits ammonium ion fluxes and seems to interact with Na/K exchanger, in which ammonium ion appear to substitute for K.In isolated perfused posterior gills , amiloride affected sodium fluxes and transbranchial potentials, supporting the possible presence of an Na/H exchanger.Branchial cell acidification was studied in Carcinus hemilamellae microspectrofluorimetrically by using the fluorescence dye BCECF.In the case when hemilamellae were immersed in Na-free solution, intracellular acidification was not affected by amiloride. In addition, in perfused gill epithelia in DSW (dilute sea water, 200 mM Na) identical with a bathing solution, acetazolamide-inhibited acidification of the perfused medium was observed.The effects of the inhibitors on the transbranchial potentials (TBP) and their role in the ion transport mechanisms of the crustacean gill are reported.
Keywords: Ionic transport, perfused gills, cuticle, Crustacea,ammonia fluxes, unidirectional sodium fluxes, acidification,acetazolamide.

Title:

Authors:: Lucu, Čedomil (26622); Towle, David

Journal: J. Exp. Zool.
ISSN: 0022-104
Volume: 265
Year: 1993
Pages: from 343 to 345
Number of references: 1
Language: engleski
Summary: The experimental use of aquatic organisms has a deep impact for understanding the biological role of transport processes,volume regulation, and acid-base regulation.The "August Krogh Principle" that for a large number of problems there will be some animal of choise on which it can be most conveniently studied,has found full support in presenday animal physiology studies. Example of this principle was summarized in an international symposium on "Transport processes across surfaces of aquatic organisms" held June 26-28, 1991 in Rovinj.Critical overview on the molecular,cellular and organ level studies of the transport phenomena were briefly presented.In the issue of The Journal of Experimental Zoology thus highlights how comparative studies of trasnsport processes in aquatic animals can aid our understanding of the mechanisms by which these organisms interact with and adapt to their environment.
Keywords: ionic regulation, volume regulation, inorganic and organic osmolytes.

Title:

Authors:: Lucu, Čedomil (26622)

Journal: J. Exp. Zool.
ISSN: 0022--104
Volume: 268
Year: 1994
Pages: from 339 to 346
Number of references: 35
Language: engleski
Summary: Preparation of posterior gills isolated from postmoult shore crab Carcinus that had been acclimated to dilute seawater were used for calcium-transport studies.The unidirectional influx of calcium significantly exceeded the efflux indicating a net trans-gill influx of calcium.Calcium influxes were inhibited when lanthanum ions were added apically. The Hill coefficient (2.66) suggested that there are at least two or three Ca carrier systems.The inhibitory action of La ions at the basolateral gill surfaces is discussed in terms of an effect on ATP-dependent calcium transport and the Na/Ca exchanger.
Keywords: Calcium transport, isolated gills, Carcinus,inhibitors, LaCl3,basolateral surface, Na/Ca exchanger.

Title:

Authors:: Flik, Gert; Verbost, Pieter; Atsma, Wim; Lucu, Čedomil (26622)

Journal: J. exp. Biol.
ISSN: 0022-0949
Volume: 195
Year: 1994
Pages: from 109 to 122
Number of references: 35
Language: engleski
Summary: A procedure was developed for the preparation of inside-out vesicles from plasma membranes from branchial epithelium of the marine organism.Gill preparation of the crab containing 22 % inside-out vesicles, 33% right-side-out vesicles and 45% leaky membrane fragments. The relative importance of Ca ATPase and Na/Ca exchanger in the branchial epithelium of Carcinus were evaluated.In such membrane vesicle preparation, the first direct evidence for uphill Ca-ATP dependent transport in Crustacea was described.
Keywords: Crab, gills, calcium transport, plasma membrane, vesicles, Ca-ATPase and Na/Ca exchanger.

Title:

Authors:: Siebers, Dietrich; Lucu, Čedomil (26622); Bottcher, Kirstin; Jurs, Karl

Journal: J. Comp. Physiol.
Volume: 164
Year: 1994
Pages: from 16 to 22
Number of references: 21
Language: engleski
Summary: Isolated posterior gills of the crab Carcinus acclimated to dilute seawater were bathed and perfused with 50% seawater.During a single passage through the gill the pH of the perfusion medium decreased from ca 8.1 to ca 7.7, a result implying that the gill possesses structures which recognize unphysiologically high pH values in the haemolymph and regulates them down to the physiological pH.The findings obtained suggest that pH regulation by crab gills depends on the presence of HCO3 ion in the perfusate as well as on oxidative metabolism of the intact epithelium where enzyme carbonic anhzdrase plays a central role.
Keywords: Carbonic anhzdrase, gill, pH regulation, perfusion, Na,K ATPase.

Title:

Authors:: Lucu, Čedomil (26622); Siebers, Dietrich

Journal: Helgolander Meeresunters.
Number: 1-4
ISSN: 0174-3597
Volume: 49
Year: 1995
Pages: from 709 to 713
Number of references: 16
Language: engleski
Summary: In a preparation of isolated gills of the shore crab Carcinus perfused with dilute seawater (pH,8.1) which was identical to the bathing solution of the gill, acidification of the collected perfusate was observed.Acidification was not effected bz 0.1 mM EIPA, however acidification was greatly blocked bz acetazolamide. The significant decrease of the acid load of the perfusate is considered to be a result of inhibition of the branchial carbonic anhyzdrase catalyzying the formation of H ions.
Keywords: Shore crab Carcinus, isolated gills, acidification, EIPA, acetazolamide, carbonic anhydrase.

Title:

Authors:: Devescovi, Massimo (109216); Lucu, Čedomil (26622)

Journal: Mar. Ecol. Prog. Ser.
ISSN: 0171-8630
Volume: 120
Year: 1995
Pages: from 169 to 174
Number of references: 27
Language: engleski
Summary: Seasonal changes in copper content of the shore crab Carcinus was studied.A significant decrease in copper content in the haemolymph and hepatopancreas and an increase in the copper space occured in winter.These changes are closely related to the drops in the protein and copper haemolymph concentration in the winter crab population.On the basis of the haemolymph copper determinations a model of the copper space distribution is described.
Keywords: Copper content, copper space, seasonal variations, Carcinus mediterraneus.

Title:

Authors:: Lucu, Čedomil (26622); Obersnel, Vojko (133632)

Journal: J. exp. Biol.
Number: ort
ISSN: 0373-434X
Volume: Rapp
Year: 1995
Pages: from 63 to 63
Number of references: 107
Language: engleski

Title:

Authors:: Pavičić, Dijana (900622); Lucu, Čedomil (26622)

Journal: J. exp. Biol.
Number: ort
ISSN: 0373-434X
Volume: Rapp
Pages: from 158 to 158
Number of references: 107
Language: engleski


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