- Type of paper
: Paper in journal
Title:
- Authors:
- Siebers, Dietrich
- Lucu, Čedomil (26622)
- Bottcher, Kirstin
- Jurs, Karl
Journal: Verh.Dtsch. Zool. Ges.
Volume: 85
Year: 1992
Pages: from 44 to 45
Number of references: 5
Language: engleski
Summary: Isolated posterior gills of shore crab Carcinus maenas
acclimated to brackish water were bathed with medium (200 mM Na) collecting
the internal perfusate during subsequent periods of 5 min.During a single
passage through the gill the pH of the perfusion medium decreased from 8.1
to 7.7, a result implying that the gill posseses structures of
"recognizing" unphysiologically high pH values of the hemolymph and
regulating them down to physiological values of 7.7. The calculated
apparent proton fluxes from the epithelial cells into the hemolymph space
amounted to 17,9 micromol/h per gram fresh weight, a value of only 3.8% of
net sodium fluxes observed under comparable conditions.When 0.1mM KCN, an
inhibitor of cytochrome oxydase, or 0.5 mM ouabain,a specific inhibitor of
Na,K aTPase were applied in the internal perfusate down-regulation of pH
was no longer observed and gill was completelly depolarized, i.e.
transbranchial potential difference dropped from 7.8 to 0 mV. The results
obtained suggest that pH regulation by crab gill depends on the intact
metabolism of the branchial epithelium and that Na/H exchanger seems to be
not involved in these processes.
Keywords: Isolated posterior gills, brackish-water crab Carcinus, pH regulation, ouabain, proton secretion.
- Type of paper
: Paper in journal
Title: Transport across ionic channels of cell membranes
- Authors:
- Lucu, Čedomil (26622)
Journal: Priroda
Number: 7
ISSN: 0351-0622
Year: 1992
Pages: from 12 to 15
Language: hrvatski
Summary: In this article there was briefly on the popular way
presentednew knowledge on the ionic channels in biological systems.For
thediscoveries in this field was in 1991 won Nobel Price inPhysiology.
Keywords: Clamp technique,ionic channels, receptors
- Type of paper
: Paper in journal
Title: Ionic regulation in marine organisms
- Authors:
- Lucu, Čedomil (26622)
Journal: Priroda
Number: 10
ISSN: 0351-0622
Volume: 80
Year: 1991
Pages: from 32 to 33
Language: hrvatski
Summary: In this scientific communication the ionic and osmotic
regulatory mechanisms of the brackish-water and marine organisms were
described.
Keywords: Hypo and hyper-osmotic regulators, ionic and osmotic regulation,marine brackish-water organisms.
- Type of paper
: Paper in journal
Title: Radioecologica aspects of radioactivity in the Adriatic
Sea.
- Authors:
- Jelisavčić, Olga (18511)
Journal: Priroda
Number: 10
ISSN: 0351-0622
Volume: 80
Year: 1991
Pages: from 0 to 0
Language: hrvatski
Summary: In this popular-scientific communication the general
principles of the radioecology were presented.In addition, few results on
radioactivity measurement in the Adriatic Sea were described.
Keywords: Radionuclides, natural and artificial radioactivity, Adriatic Sea.
- Type of paper
: Paper in journal
Title:
- Authors:
- Fuks, Dragica
- Precali, Robert
- Devescovi, Massimo (109216)
Journal: Acta Adriat.
Number: 1-2
Volume: 34
Year: 1994
Pages: from 21 to 28
Number of references: 17
Language: engleski
Summary: Spatial bacterial abundance and production in the
stratified Krka River estuary were analyzed in the fall of 1990.The
bacterial abundance within the estuary was several times higher in the open
central Adriatic waters, but was lower than in the mostly eutrophicated
western part of the northern Adriatic.Halved drop (35%) of bacterial number
between the brackish and seawater layers compared to a 60 % decrease of the
flagellates number indicated a net bacterial production below the halocline
which grayzing was unable to match.A prevailing bacterial remineralization
in the seawater layer is supposed.
Keywords: Bacterial abundance, central and northern Adriatic, chlorophyll , phytoplankton biomass.
- Type of paper
: Paper in journal
Title:
- Type of paper
: Paper in journal
Title:
- Authors:
- Medaković, Davorin (187895)
- Lucu, Čedomil (26622)
Journal: Period. Biol.
Number: 4
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 452 to 454
Number of references: 11
Language: engleski
- Type of paper
: Paper in proceedings
Title:
- Authors:
- Lucu, Čedomil (26622)
- Obersnel, Vojko (133632)
Proceedings title: Mediterranean Action Plan technical Reports series No. 52
Language: engleski
Place: Atena, Grčka
Pages: from 55 to 61
Summary: Sublethal and lethal toxicity tests of heavy metals on two
species of marine invertebrates were performed.In addition, transport of
Cs, Cd and Hg across the isolated gill epithelia,used as a modelling system
in the metal transport was studied.Under the continuous flowing test
conditions, lethal toxicity concentration of the methyl mercury (96h LC-50
= 0.031mg/l) is more than one order of magnitude lower than that for the
mercuric chloride (96hLC-50=1.04 mg/l).Influxes of radioactive labeled
mercurial compounds and caesium were studied across perfused isolated gills
of the shore crab Carcinus mediterraneus.The results show an increased
transfer of methylmercury over inorganic form across perfused epithelial
cells.Caesium net transport was directed from the hemolymph side crossingm
basolateral and apical surfaces into the bathing medium.Methylmercury
compound had reduced transbranchial potential difference values (-3.6 mV in
the control group) close to the zero.
After 1-8 days of exposure of the sea urchins Sphaerechinus granularis in
the 10 mg Cd/l sea water, gametes were isolated and fertilized. Most of
them (3-8 days exposure) exhibited delay in development, irregular
fertilization membrane formation in the early prism stage and swimming
blastula.
Keywords: Sublethal and lethal toxicity, mercury, Cs, Cd and Hg transport,gill epithelium, sea urchins, development.
- Type of paper
: Paper in proceedings
Title: Radioecological research in northern and middle Adriatic
sea before and after Chernobyl.
- Authors:
- Triulzi, Cesare
- Tassi Pelati, Laura
- Nonnis-Marzano, Francisco
- Jelisavčić, Olga (18511)
Proceedings title: Atti 12 Congresso Naz. S.I.T.E.
Language: talijanski
Place: Arcavaccata di Rende, Italia
Year: 1991
Pages: from 293 to 299
Meeting: Congresso Naz. S.I.T.E., Atti:12
Held: from 10/28/90 to 11/01/90
Summary: Results concerning determination of Cs-137 and other
artificial and natural radionuclides in Northern and MiddleAdriatic samples
of seawater (at different depths) plankton, pelagic,benthic organisms and
sediments, obtained by means of gammaspectrometry, are presented and
discussed in this paper.
Different marine specimens have been collected since 1979 during
Oceanographic field trips executed within the ASCOP(Adriatic Scientific
Cooperative Program) with Italian and Croatian research ships.
Right after the Chernobyl event (26.04.1986) significant sampling and
radionuclides determination have been made on marine species (1986-1987).
The results allow to underline a representative picture of theCs-137
concentrations in the considered sea water, plankton,organisms and sediment
samples.
Keywords: Radioecological research, Natural and artificial radioactivity,Adriatic, Cs-137
- Type of paper
: Paper in proceedings
Title:
- Authors:
- Medaković, Davorin (187895)
- Lucu, Čedomil (26622)
- Editors
- Medaković, Davorin (187895)
Proceedings title: Periodicum Biologorum
Language: engleski
Place: Zagreb
Year: 1994
Pages: from 452 to 454
Meeting: 5th Congress of Croatian Biologists
Held: from 10/03/94 to 10/07/94
Summary: The activity of enyzme carbonic anhydrase in mollusc
Mytilus edulis from the early embryonic stage to the formation of veliger
larvae was studied.The present results indicate that maximum carbonic
anhydrase activity in the D-shaped veliger at the end of forming of
prodissoconch is correlated with biomineralization process of bivalve
shells.
Keywords: Mussel Mytilus edulis,carbonic anhydrase,embryonic stages.
- Type of paper
: Paper in proceedings
Title: Concentration of Cs-137 in sea water and sediment in the
North and Middle Adriatic
- Authors:
- Jelisavčić, Olga (18511)
- Pavičić, Dijana (900622)
- Editors
- Bottcher, Kirstin
- Franić, Zdenko
- Kubelka, Dragan
Proceedings title: Zbornik radova Drugog Simpoyija Hrvatskog društva za zaštitu od zračenja
Place: Zagreb
Year: 1994
Pages: from 85 to 91
Meeting: Drugi simpozij Hrvatskog društva za zašttitu od zračenja
Held: from 11/23/94 to 11/25/94
- Type of paper
: Paper in proceedings
Title:
- Type of paper
: Paper in proceedings
Title:
- Authors:
- Lucu, Čedomil (26622)
- Obersnel, Vojko (133632)
Proceedings title: Rapport du XXXIVe Congres de la CIESM
Language: engleski
Place: Monaco, Principality of Monaco
Year: 1995
ISBN/ISSN: 0373-434X
Pages: from 63 to 63
Meeting: XXXIV Congres de la Commission Internationale pour L' Exploration Scientifique de la Mer Mediterranee
Held: from 03/27/95 to 03/31/95
- Type of paper
: Paper in proceedings
Title:
- Authors:
- Pavičić, Dijana (900622)
- Lucu, Čedomil (26622)
Proceedings title: Rapport du XXXIV e Congres de la CIESM
Language: engleski
Place: Monaco, Principality of Monaco
ISBN/ISSN: 0373-434X
Pages: from 158 to 158
Meeting: XXXIV Congres de la Commission Internationale pour L' Exploration Scientifique de la Mer Mediterranee
Held: from 03/27/95 to 03/31/95
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Siebers, Dietrich
- Lucu, Čedomil (26622)
Proceedings title: The Chalange to Marine Biology in a Changing World
Place: Hamburg, Njemačka
Year: 1992
ISBN/ISSN: 0174-3597
Pages: from 198 to 199
Meeting: 100 Years Biologische Anstalt Helgoland, The Challange in a Changing World, Scientific Symposium, September 13-18, 1992, Isle of Helgoland, Germany
Held: from 09/13/92 to 09/18/92
Summary: Crustacean gills are multifunctional organs operating in
exchanges of respiratory gases, osmo- and ionoregulatory active uptake,
regulation of the acid-base status of the hemolymph, and excretion of
nitrogenous metabolic end products. Following previous work on active ion
uptake across crab gill this work is devoted to pH -regulation by and
excretion of ammonia across the gills of the shore crab Carcinus maenas.We
were interested to answer whether H and/or HCO3 (pH regulation) and NH3
and NH4(N-excretion) are regulated across the gill epithelium using
separate mechanisms or utilize existing pathways for ion translocation.
Isolated posterior gills of brackish-water (10 x 10-3, salinity) shore
crabs were perfused and bathed with dilute sea water (200mM Na). We
observed that the pH of the perfusate (pH=8.1) after a single passage
through the gills was regulated down by ca. 0.4 pH units exhibiting
physiological means of pH 7.7.
Regulation of pH was suppresed when 0.1 mM KCN or 5 mM ouabain, aspecific
inhibitor of Na,K ATPaze, was applied in the perfusate.Regulation of pH was
also suppressed when 10-4 M acetazolamide was applied in the
perfusate.Regulation of pH remained unchanged when 10-4M amiloride or 10-5
M EIPA inhibitors of Na channels andNa/H exchangers, were applied in the
bath and in the perfusate orin the bath alone.The results suggests that pH
regulation depends of the Na,K ATPase in cooperation with a basolateral
Na/HCO3 from hemolymph into the epithelial cell utilizing the existing Na
gradient and thus decreases hemolymph pH. Findings in this work suggests
that nitorgen is excreted in the form of NH4 ions partially via the
basolateral Na,K ATPaze and as a major proportion via apical nonspecific Na
channels.
Keywords: pH regulation,perfused gill epithelium, Crustacea,transport mechanisms , NH4,extracellular acidification, H ions.
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Lucu, Čedomil (26622)
- Siebers, Dietrich
Proceedings title: The Challange to Marine Biology in a Changing World
Language: engleski
Place: Hamburg, Njemačka
Year: 1992
ISBN/ISSN: 0174-3597
Pages: from 124 to 125
Meeting: 100 Years Biologische Anstalt Helgoland, The Challange in a Changing World, Scientific Symposium, September 13-18, 1992, Isle of Helgoland, Germany
Held: from 09/13/92 to 09/18/92
Summary: Electrical resistance of the gill lamella and the
intracellularacidification of the Carcinus gill cells were studied.
Theposterior gill lamella resistance measured by the Ussings type ofchamber
was 60 Ohm cm2. The lamella resistance is significantlyincreased in the
presence of 10-4M amiloride in the bathingsolution.
The lamella was splitted out to make a feasible access to thebasolaterally
oriented cell surfaces for the intracellularacidification measurements. For
this purpose the cells wereloaded in the fluorescent dye BCECF and
acidified by 30 mM NH4Clpulses.After a sequential cell washing by different
solutions theintracellular acidification rate was
measuredmicrospectrofluorimetrically.New informations about sodiumdependent
and amiloride non-sensitive intracellular acidificationof the Carcinus gill
epithelium cells were described.
Additionally,in the isolated gill preparation perfused and bathedwith the
identical dilute sea water medium,acidification of thecollected perfusate
was observed. The acidification rate was notaffected by the EIPA (amiloride
derivative), a strong inhibitorof Na/H exchanger.The cuticle and the
underlying epithelium celllayer are two selective barriers for the
osmolytes of thecrustacean gills. The results suggest the presence of
Na/HCO3exchanger, a mechanism which is located probably on thebasolateral
side of the Carcinus gill epithelium.
Keywords: isolated gill lamella, perfused gills, shore crab, Carcinus,BCECF, EIPA, intracellular acidification, Na/HCO3 exchange.
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Lucu, Čedomil (26622)
Proceedings title: Society for Experimental Biology, Canterbery Meeting
Pages: from 0 to 0
Meeting: Annual Meeting of the Society for Experimental Biology, Centerbery Meeting
Held: from 03/19/93 to 04/02/93
Summary: A procedure for the preparation of inside-out oriented
vesicles(IOV) of isolated plasma membranes of the shore crab Carcinus
maenas was described. The presence of the enyzme Ca-ATPase and of the Na/Ca
exchanger in the membrane vesicles was found.
Keywords: Plasma membranes, shore crab Carcinus, membrane orientation,CaATPase, Na/Ca exchanger.
- Type of paper
: Summary in proceedings
Title: The role of cell respiration in ion transport of the
isolated perfused gill of the brackish water crab Carcinus
- Authors:
- Pavičić, Dijana (900622)
- Lucu, Čedomil (26622)
Proceedings title: Zbornik sažetaka priopćenja s petog kongresa biologa Hrvatske
Language: hrvatski
Place: Zagreb
Year: 1994
Pages: from 326 to 327
Meeting: 5th Congress of Croatian Biologists
Held: from 10/03/94 to 10/07/94
- Type of paper
: Summary in proceedings
Title: Seasonal changes of the copper level in the shore crabs
Carcinus mediterraneus Csrn.
- Authors:
- Devescovi, Massimo (109216)
- Lucu, Čedomil (26622)
Proceedings title: Zbornik sažetaka priopćenja s petog kongresa biologa Hrvatske
Language: hrvatski
Place: Zagreb
Year: 1994
Pages: from 327 to 328
Meeting: 5th Congress of Croatian Biologists
Held: from 10/03/94 to 10/07/94
- Type of paper
: Summary in proceedings
Title: Effects of calcium on cadmium influx across isolated gill
epithelium of the shore crab Carcinus mediterraneus Csrn.
- Authors:
- Obersnel, Vojko (133632)
- Lucu, Čedomil (26622)
Proceedings title: Zbornik sažetaka priopćenja s petog kongresa biologa Hrvatske
Place: Zagreb
Year: 1994
Pages: from 168 to 169
Meeting: 5th Congress of Croatian Biologists
Held: from 10/03/94 to 10/07/94
- Type of paper
: Summary in proceedings
Title: Marine organisms as experimental models in physiological
studies; ion transport mechanisms
- Authors:
- Lucu, Čedomil (26622)
Proceedings title: Zbornik sažetaka priopćenja s petog kongresa biologa Hrvatske
Language: hrvatski
Place: Zagreb
Year: 1994
Pages: from 300 to 302
Meeting: 5th Congress of Croatian Biologists
Held: from 10/03/94 to 10/07/94
- Type of paper
: Summary in proceedings
Title: The distribution of carbonic anhzdrase in larval and adult
mussels Mztilus edulis Linnaeus 1758
- Authors:
- Medaković, Davorin (187895)
- Lucu, Čedomil (26622)
Proceedings title: Zbornik sažetaka priopćenja s petog kongresa biologa Hrvatske
Place: Zagreb
Year: 1994
Pages: from 321 to 322
Meeting: Peti kongres biologa Hrvatske
Held: from 10/03/94 to 10/07/94
- Type of paper
: Summary in proceedings
Title:
- Type of paper
: M.A.
Title: Oxygen consumption rate in the isolated gill epithelium of
the shore crab Carcinus mediterraneus Csrn.
Faculty: Prirodoslovno-matematički Sveučilište u Zagrebu
Author: PAVIČIĆ DIJANA
Date of defense: 01/30/95
Language: hrvatski
Number of pages: 78
- Type of paper
: M.A.
Title:
- Type of paper
: Mentorship
Title: Oxygen consumption rate in gill epithelium of shore crab
Carcinus
Faculty: Prirodoslovno-matematički Sveučilište u Zagrebu
Mentor: LUCU ČEDOMIL
Date of defense: 01/30/95
Number of pages: 78
Author: Pavičić asistent Dijana
Degree level: M.A.
- Type of paper
: Mentorship
Title:
- Type of paper
: Mentorship
Title: Effect of tributhyl tin on gill epithelium acidification
of shore crab Carcinus
Faculty: Prirodoslovno-matematički Sveučilište u Zagrebu
Mentor: LUCU ČEDOMIL
Date of defense: 08/20/95
Number of pages: 52
Author: Begonja dipl. inž. kemije Ana
Degree level: D.A.
- Type of paper
: Invited lecture
Title:
Institution: Universitet Munchen
Year: 1994
- Type of paper
: Invited lecture
Title:
Institution: University of Ljubljana
- Type of paper
: Invited lecture
Title:
- Type of paper
: Invited lecture
Title: