- Type of paper
: Paper in book
Title:
- Authors:
- Mueller, Werner E.G.
- Spreitzer, I
- Milanović, Suada
- Schroeder, Heinz C.
- Gamulin, Vera (13030)
- Bretting, Hagen
- Editors
- R.W.M. Van Soest,
- T.M.G. Van Kempen,
- J.-C. Braekman,
Publisher: A.A. Balkema, Rotterdam, Brookfield
Year: 1994
Pages: from 389 to 393
Language: engleski
Other:
- Type of paper
: Paper in book
Title:
- Authors:
- Mueller, Werner E.G.
- Mueller, Isabel M.
- Gamulin, Vera (13030)
- Editors
- Mueller, Werner E.G.
Publisher: Gustav Fischer Verlag, Stuttgart, Jena, New York
Year: 1994
Pages: from 187 to 200
Language: engleski
- Type of paper
: Paper in book
Title:
- Authors:
- Gamulin, Vera (13030)
- Pfeifer, Karin
- Bretting, Hagen
- Spreitzer, I
- Mueller, Werner E.G.
- Editors
- Mueller, Werner E.G.
Publisher: Gustav Fischer Verlag, Stuttgart, Jena, New York
Year: 1994
Pages: from 213 to 223
Language: engleski
- Type of paper
: Paper in book
Title:
- Authors:
- Frank, Wolfgang
- Kruse, Michael
- Gamulin, Vera (13030)
- Rinkevich, Baruch
- Batel, Renato
- Mueller, Werner E.G.
- Editors
- Mueller, Werner E.G.
Publisher: Gustav Fischer Verlag, Stuttgart, Jena, New York
Year: 1994
Pages: from 225 to 241
Language: engleski
- Type of paper
: Paper in book
Title:
- Authors:
- Gamulin, Vera (13030)
- Editors
- Ratko Vince,
Publisher: Hrvatski radio, Zagreb
Year: 1994
Pages: from 575 to 579
Language: engleski
- Type of paper
: Paper in journal
Title:
- Authors:
- Durajlija, Sonja (170071)
- Pigac, Jasenka (37185)
- Gamulin, Vera (13030)
Journal: FEMS Microbiol. Lett.
ISSN: 0950-9232
Volume: 83
Year: 1991
Pages: from 317 to 322
Number of references: 21
Language: engleski
Summary: Two bifunctional plasmid vectors pZG5 (7.45 kb) and pZG6
(6.95kb), for gene transfer between Streptomyces spp. and Escherichiacoli
have been constructed by fusion of the multicopybroad-host-range
Streptomyces plasmid pIJ350 with E. coliplasmids Bluescribe M13- (pZG5) or
pUC18 (pZG6). Both plasmidspossess several unique restriction sites
suitable for DNAcloning. Stable transformants of Streptomyces rimosus R6
and S.lividans 66 were obtained, harboring intact plasmids regardlessof
colony age or multiple subculturing. Moreover, pZG5 and pZG6were
successfully used to introduce several homologous transferRNA genes into S.
rimosus.
- Type of paper
: Paper in journal
Title:
- Authors:
- Biesalski, Hans
- Doepner, Gudrun
- Tzimas, Georg
- Gamulin, Vera (13030)
- Schroeder, Heinz C.
- Batel, Renato
- Nau, Heinz
- Mueller, Werner E.G.
Journal: Oncogene
ISSN: 0378-1097
Volume: 7
Year: 1992
Pages: from 1765 to 1774
Number of references: 83
Language: engleski
Summary: We demonstrate that the cells of the sponge Geodia cydonium
areequipped with the basic elements required for a retinoic
acid(RA)-dependent response pathway; RA was identified andquantitated, the
cellular RA-binding protein (CRABP) was detectedand the nuclear RA receptor
(RAR) was found. In the isolated cellsystem the level of CRABP, but not of
RAR, is strongly inducedafter incubating the cells for 10h with the
homologousaggregation factor. In induced cells incubation with 0.3 microMRA
results in a strong down-regulation of the c-myb (orc-myb-related)
proto-oncogene (M(r) 63,000; mRNA 3.3 kb). Wepostulate that this pathway is
also functionally active and thatRA acts as a natural morphogen.
- Type of paper
: Paper in journal
Title:
- Authors:
- Gamulin, Vera (13030)
- Pujić, Petar (170082)
- Durajlija, Sonja (170071)
Journal: Period. biol.
Number: 4
ISSN: 0031-5362
Volume: 95
Year: 1993
Pages: from 449 to 452
Number of references: 10
Language: engleski
Summary: Streptomyces, Gram-positive mycelial soil bacteria with
complexlife cycle and morphological differentiation, posses severalclasses
of promoters which are recognized by different sigmafactors during the
process of contolled gene expression.Regulatory sequences involved in gene
expression in S. rimosusare not known and there are no reports about the
mode ofexpression of tRNA genes in Streptomyces. We recently
completedsequence analysis of rrnF operon from S. rimosus and found onlyone
(P4) promoter in front of this operon. We also analysedexpression of seven
tRNA genes from S. rimosus, two independenttRNA-fMet genes and a cluster of
five Gln-Glu genes. All threetRNA gene promoters are quite diferent.
Deletion experimentsshowed that tRNA genes in the clusted are all
cotranscribed fromthe same promoter localized 140-65 bp upstream from the
firstgene where the sequence TTGCGC-17-TAGACT was found. This tRNAgene
promoter belongs to the class of Streptomyces - E. coli typepromoters
(SEP).
- Type of paper
: Paper in journal
Title:
- Authors:
- Pfeifer, Karin
- Haasemann, Martina
- Gamulin, Vera (13030)
- Bretting, Hagen
- Fahrenholz, Falk
- Mueller, Werner E.G.
Journal: Glycobiology
ISSN: 0959-6658
Volume: 3
Year: 1993
Pages: from 179 to 184
Number of references: 42
Language: engleski
Summary: The marine sponge Geodia cydonium contains several lectins.
Themain component, called lectin-1, is composed of three to fouridentical
subunits. The subunits of the lectins were cloned froma cDNA library; two
clones were obtained. From the deduced aasequence of one clone, LECT-1, a
mol.wt of 15313 Da iscalculated; this value is in good agreement with
massspectrometric analysis of 15453+-25 Da. The sequence of anotherclone,
LECT-2, was analysed and the aa sequence was deduced(15433 Da). The two
subunits have a framework sequence of 38conserved aa which are
characteristic for thecarbohydrate-binding site of vertebrate S-type
lectins.Clustering of lectin sequences of various species following
theirpairwise comparison established a dendogram, which reveals thatthe
sponge lectin could be considered as the ancestor forvertebrate S-type
lectins.
- Type of paper
: Paper in journal
Title:
- Authors:
- Schroeder, Heinz C.
- Ugarković, Đurđica (3874)
- Gamulin, Vera (13030)
- Bek, Andreas
- Mueller, Werner E.G.
Journal: Croatian Med. J.
ISSN: 0353-9504
Volume: 33
Year: 1992
Pages: from 108 to 113
Number of references: 18
Language: engleski
Summary: The interaction of pyronin Y, an RNA intercalating drug,
with thebinding of Rev protein from human immunodeficiency virus type
1(HIV-1) to Rev responsive element (RRE)-containing env RNA wasstudied. In
a gel retardation assay, recombinant Rev proteintightly bound to in vitro
transcribed RRE RNA. At a concentrationof 1ug/ml, complex formation was
almost completely inhibited.Electron microscopy revealed that Rev
oligomerizes in thepresence of RRE-containing RNA with the formation of
shortrod-like structures of long filaments, depending on the length ofthe
transcript. The assembly of Rev protein along RRE-containingRNAs was
abolished after addition of pyronin Y. Thus pyronin Yrepresents the first
compound described to inhibit Rev-RREcomplex formation under in vitro
conditions.
- Type of paper
: Paper in journal
Title:
- Authors:
- Vujaklija, Dušica (116760)
- Hirinouchi, Sueharu
- Beppu, Teruhiko
Journal: J. Bacteriol.
ISSN: 0021-9193
Volume: 175
Year: 1993
Pages: from 26522 to 2661
Number of references: 26
Language: engleski
Summary: DNA-binding assays using mobility shift polyacrylamide
gelelectrophoresis revealed the presence of a protein thatspecifically
bound to a restriction fragment -288 to -191 bpupstream from the
transcriptional start point of strR, aregulatory gene for streptomycin
biosynthesis in Streptomycesgriseus. The binding site corresponded to an
upstream activationsequence predicted from the results of in vivo promoter
assays.The binding was greatly enhanced by 5 mM Mg++. This binding
wasdetected with the protein source only from the wild-type strainand not
from an A-factor-deficient mutant strain. The exogenoussupplementation of
A-factor to the A-factor-deficient mutantstrain caused the appearance of
the protein in the protein in theDNA-binding assay. A synthetic nucleotide
52 bp in length (regionfrom -293 to -242), which was synthesized on the
basis of dataobtained from both retardation assays with dissected
DNAfragments and in vivo promoter assays, was retarded by
theA-factor-dependent protein. In addition to thisA-factor-dependent
protein, at least three proteins withdifferent recognition site affinities
capable of binding to theupstream region of the strR promoter were
detected. The regionbound by these proteins showed anomalous
electrophoreticmobility, like that of a bent DNA molecule. This region was
foundto be transcribed in the orientation opposite to that of strR.
- Type of paper
: Paper in journal
Title:
- Authors:
- Plohl, Miroslav (3986)
- Gamulin, Vera (13030)
Journal: FEMS Microbiol. Lett.
ISSN: 0950-9232
Volume: 77
Year: 1991
Pages: from 139 to 144
Number of references: 22
Language: engleski
Summary: One Streptomyces rimosus ribosomal RNA gene set (rrnF) was
clonedinto pBR322 as 8.8 kilobase-pairs BamHI fragment and sequence ofthe
3' end of the operon with 5S rRNA gene was determined. 5SrRNA gene is 120
bp long and highly homologous with two otherknown 5S rRNA from
Streptomyces. Remarkable sequence homologywith S. ambofaciens rrnD operon
exists in the 3' noncodingregions, including first transcription
termination signal. S.rimosus rrnF operon contains second putative
terminator which isabsent in S. ambofaciens rrnD operon. tRNA genes were
not foundat the 3' end of rrnF operon. The number of rRNA operons in
S.rimosus was defined by Southern hybridization analysis. S.rimosus possess
six rRNA operons and all rRNA operons containidentical PstI fragment of 4.5
kb. rRNA genes in operons areseparated by short intergenic regions and
organized in the order16S-23S-5S. Efforts to connect tRNA genes with S.
rimosus rRNAoperons were unsuccesful.
- Type of paper
: Paper in journal
Title:
- Authors:
- Schacke, Heike
- Rinkevich, Baruch
- Gamulin, Vera (13030)
- Mueller, Isabel M.
- Mueller, Werner E.G.
Journal: J. Mol. Recognit.
Volume: 7
Year: 1994
Pages: from 273 to 276
Language: engleski
- Type of paper
: Paper in journal
Title:
- Authors:
- Schacke, Heike
- Schroeder, Heinz C.
- Gamulin, Vera (13030)
- Rinkevich, Baruch
- Mueller, Isabel M.
- Mueller, Werner E.G.
Journal: Molec. Membrane Biol.
Volume: 11
Year: 1994
Pages: from 101 to 107
Language: engleski
- Type of paper
: Paper in journal
Title:
- Authors:
- Mikoč, Andreja
- Gamulin, Vera (13030)
Journal: Acta biologica
ISSN: 0448--014
Year: 1995
Language: engleski
- Type of paper
: Paper in proceedings
Title: Investigations of cobweb-like material thrown from the
enemy aircrafts over the territory of the Republic of Croatia
- Authors:
- Furić, Krešimir
- Gamulin, Vera (13030)
- Jakšić, M.
- Jernej, Branimir
- Ladešić, Branko
- Mesarić, Štefica
- Veksli, Zorica
- Wrischer, Mercedes (54316)
- Editors
- Prpić-Majić, Danica
Proceedings title: Toksikološka služba u obrani domovine (Zbornik radova)
Language: hrvatski
Place: Zagreb
Year: 1993
Pages: from 178 to 185
Meeting: Toksikološka služba u obrani domovine
Held: from 01/21/93 to 01/22/93
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Gamulin, Vera (13030)
- Pujić, Petar (170082)
- Plohl, Miroslav (3986)
- Durajlija, Sonja (170071)
Proceedings title: Godišnji sastanak hrvatskih biokemičara (sažetak radova)
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 60 to 60
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
Other: Summary of the invited lecture
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Milanović, Suada
- Schroeder, Heinz C.
- Mueller, Werner E.G.
- Gamulin, Vera (13030)
Proceedings title: Godišnji sastanak hrvatskih biokemičara (sažetak radova)
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 84 to 84
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
Other: Summary of the poster presentation
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Mueller, Werner E.G.
- Schroeder, Heinz C.
- Gamulin, Vera (13030)
- Editors
- Mueller, Werner E.G.
Proceedings title: Use of aquatic organisms as tools for monitoring of environmental hazards
Language: engleski
Place: Mainz, Njemačka
Year: 1993
Pages: from 13 to 13
Meeting: Use of aquatic organisms as tools for monitoring of environmental hazards
Held: from 11/02/93 to 11/03/93
Other: Summary of the invited lecture
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Durajlija, Sonja (170071)
- Editors
- Putz, Joern
- Dotsch, Volker
Proceedings title: Second International Meeting: Young Scientists View of Molecular Biotechnology
Language: engleski
Place: Strasbourg, Francuska
Year: 1993
Pages: from 43 to 43
Meeting: Second International Meeting: Young Scientists View of Molecular Biotechnology
Held: from 02/28/93 to 03/06/93
Other: Summmary of the invited lecture
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Mikoč, Andreja
- Gamulin, Vera (13030)
Proceedings title: Book of Abstracts
Language: engleski
Place: Zagreb
Year: 1994
Meeting: Fifth International Summer School on Biophysics
Held: from 09/18/94 to 09/29/94
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Ćetković, Helena
- Mikoč, Andreja
- Mueller, Werner E.G.
- Gamulin, Vera (13030)
Proceedings title: Book of Abstracts
Language: engleski
Place: Zagreb
Year: 1994
Meeting: Peti Kongres biologa Hrvatske, Pula
Held: from 10/03/94 to 10/10/94
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Gamulin, Vera (13030)
- Mueller, Isabel M.
- Mueller, Werner E.G.
Proceedings title: Book of Abstracts
Language: engleski
Place: Zagreb
Year: 1994
Meeting: Peti Kongres biologa Hrvatske, Pula
Held: from 10/03/94 to 10/10/94
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Vujaklija, Dušica (116760)
- Žafran, Jelena
- Mikoč, Andreja
- Gamulin, Vera (13030)
Proceedings title: Book of Abstracts
Language: engleski
Place: Zagreb
Meeting: Peti Kongres biologa Hrvatske, Pula
Held: from 10/03/94 to 10/10/94
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Žafran, Jelena
- Redžepović, Sulejman
- Gamulin, Vera (13030)
Proceedings title: Book of Abstracts
Language: engleski
Place: Zagreb
Year: 1994
Meeting: Peti Kongres biologa Hrvatske, Pula
Held: from 10/03/94 to 10/10/94
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Mikoč, Andreja
- Gamulin, Vera (13030)
Proceedings title: Book of Abstracts
Language: engleski
Place: Zagreb
Year: 1994
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 10/14/94 to 10/15/94
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Mikoč, Andreja (199742)
- Gamulin, Vera (13030)
Proceedings title: Book of Abstracts
Language: engleski
Place: Zagreb
Year: 1995
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 09/22/95 to 09/25/95
- Type of paper
: M.A.
Title: Expression of transfer RNA genes in Streptomyces rimosus
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Date of defense: 07/02/92
Language: hrvatski
Number of pages: 55
Summary: In this work, expression of S. rimosus tRNA genes has
beenstudied in vivo in homologous system (S. rimosus) and inheterologous
system (E. coli). Seven tRNA genes were analyzed:two independent genes for
tRNA-fMet and a cluster
containing two tRNA-Gln and three tRNA-Glu genes. All S. rimosustRNA genes
were (re)cloned in different combinations andorientations, with or without
potential promoter regions, intostable bifunctional multicopy plasmid
vectors pZG5 and pZG6,especially constructed for this purpose. Recombinant
bifunctionalplasmids with tRNA genes were introduced into both hosts.
Totalcellular tRNAs were isolated from S. rimosus and E. colitransformants
containing different recombinant plasmids andsubjected to northern
hybridization analysis for the estimationof the relative amount of
tRNA-fMet, tRNA-Gln and tRNA-Glu indifferent cell lines. Northern
hybridization analysis shows thatall analyzed promoters are active in
homologous system. Fromthe deletion assay it can be concluded that all
tRNA-Gln andtRNA-Glu genes are cotranscribed from the same promoter
located140-65 bp upstream from the first gene. Sequence of this promoteris
TTGGAC-17-TAATGT. In the heterologous system (E. coli) onlythe cluster of
Gln-Glu tRNA genes is properly expressed.
- Type of paper
: M.A.
Title: Structure of rrnF ribosomal RNA operon from Streptomyces
rimosus
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Date of defense: 07/04/92
Language: hrvatski
Number of pages: 54
Summary: The primary structure of the S. rimosus rrnF ribosomal RNA
operonhas been determined. This rRNA operon is located on the 8.8 kbBamH1
fragment. Deduced nucleotide sequence ( 6067 bp)contains a promoter region,
the genes for 16S, 23S and 5Sribosomal RNAs, intergenic regions and
termination sequences.This results represents the first primary structure
of one S.rimosus RNA operon, and one of only few structures of rRNAoperons
in Streptomyces.
The 16S rRNA gene is 1529 bp long and highly conserved inrelation to four
known 16S rRNA genes from Streptomyces. Thisgene contains highly variable
regions which are specific forS. rimosus. Region between 16S and 23S rRNA
genesis 298 bp long. The 23S rRNA gene is the longestsequenced gene in
prokaryotes (3121 bp), as a result of theinsertions in the 5-end of the 23S
rRNA gene. The region upstreamfrom the 16S rRNA gene, 689 bp long, contains
potential 3- end ofone open reading frame ( last 61 amino acids). The
potentialpromoters were found in the region 268-297 bp (P4) and420-448 bp
(P3?) upstream from the 16S rRNA gene. Only P4promoter shares similarity
with other known promoters of rRNAoperons in Streptomyces. Region
downstream from P4 promoter iscomplementary to the intergenic region
between 16S and 23S rRNAgenes and most probably play an important role in
theantitermination of the transcription and processing of theprimary
transcript. In this region are found highly conservedsequences known as A
blocks.
- Type of paper
: M.A.
Title: Study of the Nodulation Genes in Autochthonous Strains of
Rhizobium leguminosarum
Faculty: Agronomski fakultet Sveučilište u Zagrebu
Author: GAMULIN VERA
Date of defense: 05/26/95
Language: hrvatski
Number of pages: 115
- Type of paper
: Mentorship
Title: Expression of transfer RNA genes from Streptomyces rimosus
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: ADLER NEVENKA
Date of defense: 07/04/92
Number of pages: 55
Author: Durajlija Mr biologije Sonja
Degree level: M.A.
- Type of paper
: Mentorship
Title: Structure of rrnF ribosomal RNA operon from Streptomyces
rimosus
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: ADLER NEVENKA
Date of defense: 07/04/92
Number of pages: 54
Author: Pujić Mr.biol Petar
Degree level: M.A.
- Type of paper
: Mentorship
Title: Study of the Nodulation Genes in Autochtonous Strains of
Rhizobium leguminosarum
Faculty: Agronomski fakultet Sveučilište u Zagrebu
Mentor: GAMULIN VERA
Date of defense: 05/26/95
Number of pages: 115
Author: Žafran magistra biol. znanosti Jelena
Degree level: M.A.
