- Type of paper
: Paper in book
Title: Genetic engeenering
- Authors:
- Petranović, Drago (36623)
- Petranović, Mirjana (36634)
- Editors
- Zergollern, Ljiljana
Publisher: Medicinska naklada
Year: 1994
Pages: from 79 to 101
Number of references: 25
Language: hrvatski
- Type of paper
: Paper in book
Title: Cell cycle of bacteria Escherichia coli
- Authors:
- Petranović, Mirjana (36634)
- Editors
- Dekaris, Drago
- Pavelić, Krešimir
- Spaventi, Radan
- Dekaris, Drago
Publisher: HAZU
Year: 1994
Pages: from 2490 to 2580
Number of references: 6
Language: hrvatski
Summary: The basic elements of cell cycle of any living cell
comprise growth followed by division of i) cytoplasm, ii) genome and iii)
cellular envelope. These processes don't procced independently to each
other, even more they are concomitant and cross-controled. Fine mechanisms
of these interactions are described for E. coli cells.
- Type of paper
: Paper in journal
Title: A possible interaction of single-strand binding protein
and RecA protein during post-ultraviolet DNA synthesis
- Authors:
- Trgovčević, Željko (50435)
- Petranović, Mirjana (36634)
- Brčić-Kostić, Krunoslav (157324)
- Petranović, Drago (36623)
- Lerš, Nella (123006)
- Salaj-Šmic, Erika (42166)
- Petranović, Drago (36623)
Journal: Biochimie
Volume: 73
Year: 1991
Pages: from 515 to 517
Number of references: 27
Language: engleski
Summary: The mechanism of DNA replication in ultraviolet
(UV)-irradiatedEscherichia coli is proposed. Immediately after UV exposure,
thereplisome aided by single-strand DNA-binding protein (SSB) canproceed
past UV-induced pyrimidine dimers without insertion ofnucleotides.
Polymerisation eventually resumes somewheredownstream of the dimer sites.
Due to the limited supply of SSB,only a few dimers can be bypassed in this
way. Nevertheless, thisearly DNA synthesis is of great biological
importance because itgenerates single-stranded DNA regions. Single-stranded
DNA canbind and activate RecA protein, thus leading to induction of theSOS
response. During the SOS response, the cellular level of RecAprotein
increases dramatically. Due to the simultaneous increasein the
concentration of ATP, RecA protein achieves thehigh-affinity state for
single-stranded DNA. Therefore it is ableto displace DNA-bound SSB. The
cycling of SSB on and of DNAenables the replisome to bypass a large number
of dimers at latepost-UV times. During this late replication, the
stoichiometricamounts of RecA protein needed for recombination are involved
inthe process of postreplication repair.
Keywords: Escherichia coli, UV radiation, post-UV DNA synthesis, SSB protein, RecA protein
- Type of paper
: Paper in journal
Title: Mechanisms of illegitimate recombination
- Authors:
- Ehrlich, S.D.
- Bierne, H.
- d'Alencon, E.
- Villete, D.
- Petranović, Mirjana (36634)
- Noirot, P.
- Michel, B.
Journal: Gene
ISSN: 0378-1119
Volume: 135
Year: 1993
Pages: from 161 to 166
Number of references: 46
Language: engleski
Summary: Illegitimate recombination, which is one of the major
causes ofgenome rearrangements, can occur in a number of ways. These
mightinvolve enzymes which cut and join DNA or enzyme which replicateDNA,
as illustrated by two examples: (i) formation of deletionsat the
replication origin (ori) of an Escherichia colibacteriophage, M13; and (ii)
excision of E. coli transposon Tn10.It is proposed that a common theme to
various ways by whichillegitimate recombination can occur might be the
capacity tocreate ends in the DNA molecule and to make the ends meet.
Keywords: recombinant DNA, genome rearrangement, copy-choice model, pausing, replication fork, hot spot, rolling circle, ori, direct repeats, transport excision
- Type of paper
: Paper in journal
Title: General recombination of lambda phage in UV-irradiated
Escherichia coli cells
- Authors:
- Vlahović, Ksenija
- Zahradka, Davor
- Petranović, Dragutin
- Petranović, Mirjana (36634)
Journal: Periodicum Biologorum
Number: 4
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 362 to 363
Number of references: 12
Language: engleski
- Type of paper
: Paper in journal
Title: Growth and division of UV-irradiated Escherichia coli
- Authors:
- Zahradka, Davor
- Vlahović, Ksenija
- Petranović, Mirjana (36634)
- Petranović, Dragutin
Journal: Periodicum Biologorum
Number: 4
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 359 to 361
Number of references: 9
Language: engleski
- Type of paper
: Paper in journal
Title: The erythrocytes and lysate of erythrocytes might mitigate
harmful effect of UVC-light on mammalian cells or bacteriophage lambda
- Authors:
- Poljak-Blaži, Marija
- Stambolija, Nadica
- Petranović, Mirjana (36634)
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 97
Year: 1995
Pages: from 35 to 40
Number of references: 25
Language: engleski
Summary: Harmful effect of UV light involves cell killing
mutagenesis and neoplastic transformation of exposed cells. Mammalian cells
are not equally sensitive to UVC light. We examined the mechanism of the
protective role of erythrocytes and lysed erythrocytes on mononuclear cells
lysis and on their colony forming ability aftere UVC irradiation. The
bacteriophage lambda was also used for evolution of the protective role of
RBC lysate from the UVC light. Hemolysis was determined measuring the
haemoglobin in the supernatants immediately after the UVC irradiation.
Smears of the sediment were stained and differential counts were
determined. The bone marrow cells were propagated in vivo for determination
of the colony forming ability. High fluences of UVC caused hemolysis of the
erythrocytes and reduced the number of nucleated blood cells to 60%. The
erythrocytes, as well as the substance(s) from lysed erythrocytes
(predominantly haemoglobin), could protect the bone marrow stem cells from
UVC fluences as high as 16 kJ/m2. The phage survival is about 3700 times
higher if the phages were irradiated in supernatant of hemolysed
erythrocytes. The phage survival was even higher (about 6300 times) if the
supernatant of hemolysed erythrocytes was previously irradiated (with 4
kJ/m2).
Keywords: UVC light, hemolysis, bone marrow cells, phage lambda, E. coli
- Type of paper
: Summary in proceedings
Title: Mismatch repair in Xenopus egg extracts is not
methyl-directed
- Authors:
- Petranović, Mirjana (36634)
- Radman, Miroslav
- Editors
- Taylor, A.
Proceedings title: Cell Cycle Checkpoints, DNA Repair and DNA Replication Strategies
Language: engleski
Place: Cambridge, Engleska
Year: 1993
Pages: from 147 to 147
Meeting: Cell Cycle Checkpoints, DNA Repair and DNA Replication Strategies
Held: from 09/27/93 to 10/01/93
Summary: DNAs from phage mutants M13mp18 and M13mp18/MP-1 were used
to construct two closed circular heteroduplexes (M. Petranović et
al./1990/Nucl.Ac.Res. 18: 2159). One of them carried the sequence
5'-CCTGGG-3', 3'-GGGCCC-5' with a T-G mismatch at the position 6248. The
other carried the sequence 5'-CCCGGG-3', 3'-GGACCC-5' with a C-A mismatch
at the same position. Heteroduplexes were unmethylated, methylated only in
one strand or methylated in both strands. For methylation HpaII methylase
was used. (It adds a methyl group to the position 5 of the second C in
5'-CCGG-3' sequences of the double-stranded DNA). Incubation of
heteroduplexes with Xenopus egg extract resulted in the repair of a
significant fraction of molecules containing either a T-G or a C-A mispair.
Upon repair both mispairs gave preferentially C-G pairs and this bias was
not influenced by the methylation pattern of heteroduplexes.
- Type of paper
: Summary in proceedings
Title: Some restriction endonucleases tolerate single mismatches
of the pyrimidine-purine type
- Authors:
- Petranović, Mirjana (36634)
- Petranović, Drago (36623)
- Radman, Miroslav
Proceedings title: Godišnji sastanak hrvatskih biokemičara
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 116 to 116
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
Summary: DNAs from phage mutants M13mp18 and M13mp18/MP1 were used
to construct two closed circular heteroduplexes. One of them carried the
sequence 5'-CCTGGG-3' 3'-GGGCCC-5' with a T-C mismatch at the position
6248. The other carried the sequence 5'-CCCGGG-3' 3'-GGACCC-5' with a C-A
mismatch at the same position. Heteroduplexes were exposed to 7 restriction
endonucleases having recognition sites within the sequence 5'-CCCGGG-3'
3'-GGGCCC-5' and to 1 restriction endonuclease having a recognition site
within the sequence 5'-CCTGGG-3' 3'-GGACCC-5'. All tested enzymes cleaved
at least one mismatch-containing seguence although with reduced efficiency.
SmaI and XmaI tolerated both mismatch-containing sequences. AvaI, HpaII,
MspI, NciI and NspIII were able to tolerate only the T-G containing
sequence, while BstI was able to tolerate only the C-A containing sequence.
It is inferred that the tolerance displayed by SmaI and XmaI depends on
presence od either the original purines or the original pyrimidines in
mismatches of both the T-G and C-A type and that another tested enzymes
require the presence of the original purines in mismatches of both types.
- Type of paper
: Summary in proceedings
Title: Growth and division of UV-irradiated Escherichia coli
cells
- Authors:
- Zahradka, Davor
- Petranović, Mirjana (36634)
- Petranović, Dragutin
Proceedings title: Peti kongres biologa Hrvatske - Zbornik sažetaka priopćenja
Language: hrvatski
Place: Zagreb
Year: 1994
Pages: from 77 to 78
Meeting: Peti kongres biologa Hrvatske
Held: from 10/03/94 to 10/07/94
Summary: Cell survival, cell number and cell volume distribution
measurements were used to study postirradiation growth and division
kinetics of surviving and non-surviving cells of E. coli K-12. Cells were
lysogenized with a thermoinducible cI mutant of lambda phage and their
dying after irradiation was also probed by measuring thermoinducibility.
The results show that in all strains the division is much more inhibited by
irradiation than the growth. As a consequence, the mean cell volume
increases and the fraction of filaments appears in irradiated populations.
In wild type strain as well as in sfiA sfiC mutants the subpopulation of
filaments consists mainly of viable cells. Filaments usually contain
several copies of bacterial chromosome, which indicates that DNA
replication recovery is not sufficient for the recovery of cell division.
SOS genes sfiA and sfiC, known to encode cell division inhibitors, do not
influence division od the irradiated cells. Also, their action on viability
appears to be without any effect in the irradiated cells. The division
inhibition is much more pronounced in recA and recB mutants in comparison
to rec+ lexA(Ind) mutant, which suggest that recombination proteins may
play a role in the regulation of postirradiation cell division.
- Type of paper
: Summary in proceedings
Title: General recombination of lambda phage in UV-irradiated
Escherichia coli cells
- Authors:
- Vlahović, Ksenija
- Petranović, Dragutin
- Petranović, Mirjana (36634)
Proceedings title: Peti kongres biologa Hrvatske - Zbornik sažetaka priopćenja
Language: hrvatski
Place: Zagreb
Year: 1994
Pages: from 78 to 79
Meeting: Peti kongres biologa Hrvatske
Held: from 10/03/94 to 10/07/94
Summary: The ability of lambda phage to participate in general
recombination in UV-irradiated Escherichia coli cells was investigated in
prophage x phage and phage x phage crosses. It was found that the prophage
in irradiated wild type cells progressively loses the ability to recombine
with the superinfecting phage. General recombination in prophage x phage
crosses was inhibited on Red-dependent and RecF-dependent or RecF-analogous
recombination pathways. The experiments with E. coli recB mutants have
shown that the functional RecBCD enzyme is required for the inhibition of
prophage general recombination. Phage x phage crosses have shown that the
inhibitor of general recombination acts along the irradiated bacterial
chromosome but not in the irradiated cytoplasm. Since the process of
inhibition develops in dying cells and since it depends on the functional
RecBCD enzyme, it is concluded that the postirradiation inhibition of
general recombination in prophage x phage crossses is a consequence of the
unsuccessful RecBCD-dependent recombination repair of bacterial DNA.
- Type of paper
: Ph.D.
Title: Genetic evidence for the existence of antirecombinases in
Escherichia coli
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Author: PETRANOVIĆ DRAGO
Date of defense: 11/26/93
Language: hrvatski
Number of pages: 110
Summary: Experimental system consisting of lambda phage and E. coli
cellshas been used to study the genetics of DNA recombination andrepair.
The experiments have revealed the existence of twoantirecombinases in
irradiated SOS-induced cells. One is RecBCDhelicase activity. In lethally
damaged cells it antagonizesInt-dependent site-specific recombination in
profage x bacterialchromosome crosses and Red-dependent and/or
RecF-dependentgeneral recombination in prophage x phage crosses. The
otherantirecombinase is helicase II. At the elevated levels such asthose in
irradiated SOS-induced cells, it antagonizesRecF-dependent multiplicity
reactivation of irradiated phage.
- Type of paper
: M.A.
Title: Growth and division of irradiated Escherichia coli cells
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Author: ZAHRADKA DAVOR
Date of defense: 07/04/94
Language: hrvatski
Number of pages: 71
Summary: Growth and division of UV-irradiated E. coli K-12 cells
were studied in this work. In addition to wild type strain, sfiA sfiC,
recA, recB and lexA(Ind) mutants were used in experiments. Cell growth was
moderately slowed down in all irradiated bacterial cultures. Cell divisino
was markedly inhibited in majority of irradiated strains and, as a result,
filamentous cells appeared. A method which enables separate study of viable
and nonviable cells was used. Obtained results show that cell dying after
UV is not caused by division inhibition or filamentation. In wild type
strain as well as in sfiA sfiC mutants the subpopulation of filaments
consists mainly of viable cells. Filaments usually contain several copies
of bacterial chromosome indicating that DNA replication recovery is not
sufficient for the recovery of cell division. SOS genes sfiA and sfiC,
known to encode cell division inhibitors, do not influence division of the
irradiated cells. Also, their action on viability appears to be without any
effect in the irradiated cells. the division inhibition is much more
pronounced in recA and recB mutants in comparison to rec+ lexA(Ind) mutant,
which suggests that recombination proteins may play a role in the
regulation of postirradiation cell division.
- Type of paper
: M.A.
Title: Recombination of lambda phage in irradiated Escherichia
coli
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Author: VLAHOVIĆ KSENIJA
Date of defense: 07/04/94
Language: hrvatski
Number of pages: 82
Summary: The ability of lambda phage to participate in general
recombination in UV-irradiated Escherichia coli cells was investigated in
prophage x phage and phage x phage crosses. It was found that the prophage
in irradiated wild type cells progressively loses the ability to recombine
with the superinfecting phage. General recombination in prophage x phage
crosses was inhibited on Red-dependent and RecF-dependent or RecF-analogous
recombination pathways. The experiments with E. coli recB mutants have
shown that the functional RecBCD enzyme is required for the inhibition of
prophage general recombination. Phage x phage crosses have shown that the
inhibitor of genral recombination acts along the irradiated bacterial
chromosome but not in the irradiated cytoplasm. Since the process of
inhibition develops in dying cells and since it depends on the functional
RecBCD enzyme, it is concluded that the postirradiation inhibiton of
general recombination in prophage x phage crosses is a consequence of the
unsuccessful RecBCD-dependent recombination repair of bacterial DNA.
- Type of paper
: Mentorship
Title: Programmed cell death
Faculty: Farmaceutsko-biokemijski fakultet Sveučilište u Zagrebu
Mentor: PETRANOVIĆ MIRJANA
Number of pages: 47
Author: Cvitković Bernard
Degree level: D.A.
- Type of paper
: Mentorship
Title:
Faculty: Farmaceutsko-biokemijski fakultet Sveučilište u Zagrebu
Mentor: PETRANOVIĆ MIRJANA
Number of pages: 54
Author: Šodan Mirela
Degree level: D.A.
- Type of paper
: Mentorship
Title:
Faculty: Farmaceutsko-biokemijski fakultet Sveučilište u Zagrebu
Mentor: PETRANOVIĆ MIRJANA
Number of pages: 53
Author: Zeman Biljana
Degree level: D.A.
- Type of paper
: Mentorship
Title: Recombination of lambda phage in irradiated Escherichia
coli
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: PETRANOVIĆ MIRJANA
Date of defense: 07/04/94
Number of pages: 82
Author: Vlahović Ksenija
Degree level: M.A.
- Type of paper
: Mentorship
Title: Growth and division of irradiated Escherichia coli cells
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: PETRANOVIĆ MIRJANA
Date of defense: 07/04/94
Number of pages: 71
Author: Zahradka Davor
Degree level: M.A.
- Type of paper
: Project
Title: Genes and enzymes involved in the inhibition of
recombination
- Authors:
- Petranović, Drago (36623)
Ordering party: ICGEB/UNIDO
Institution depot: ICGEB/UNIDO
Year: 1993
- Type of paper
: New breed
Title: New Escherichia coli and lambda strains
- Authors:
- Petranović, Drago (36623)
- Petranović, Mirjana (36634)
- Vlahović, Ksenija
- Zahradka, Davor
Other: U nasem laboratoriju za potrebe istraživačkog rada
konstruirali smo desetak novih sojeva bakterije Escherichia coli, faga
lambda te plazmida.
- Type of paper
: Other
Title: Research proposal for collaborative research programme
ICGEB/UNIDO: Genes and enzymes involved in the inhibition of recombination
- Authors:
- Petranović, Drago (36623)
Type of work: znanstveni projekt
Language: engleski
- Type of paper
: Other
Title: First Session of the Board of Governors of the
International Centre for Genetic Engineering and Biotechnology, Trieste,
Italy, 3-5 October 1994
- Authors:
- Petranović, Drago (36623)
Type of work: Organizacijski i strucni
