SVIBOR - Project code: 1-08-208

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 1-08-208


REGULATION OF DNA RECOMBINATION AND RECOMBINATIONAL DNA REPAIR


Main researcher: PETRANOVIĆ, DRAGO (36623)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/93.
Papers on project (total): 24
Papers on project quoted in Current Contents: 3
Institution name: Institut "Ruđer Bošković", Zagreb (98)
Department/Institute: Laboratory of molecular microbiology, Department of molecular genetics
Address: Bijenička c. 54
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 434-255
Fax: 385 (0)1 425-497
E-mail: kvlahovic&olimp.irb.hr
Summary: Experimental systems consisting of E. coli cells, phages and plasmids will be used to study the genetics, molecular mechanisms and biological effects of DNA recombination and recombinational DNA repair. Special attention will be paid to the processes influencing the frequency of recombination and the efficiency of recombinational repair. One of such processes leads to the inability of lambda prophage to take a part in some types of site-specific and general recombination. The process seems to be confined to the chromosome of irradiated cells and to be associated with the postreplication type of recombinational repair. Another inhibitory process was found to occur in the cytoplasm of irradiated cells. It is responsible for the inability of multiply infected cells to reactivate the irradiated phage lambda by a type of recombinational repair called multiplicity reactivation. We also plan to participate in the studies of postirradiation synthesis of chromosomal DNA and synthesis of single stranded plasmid DNA. Postirradiation DNA synthesis attracts our attention since it is involved in the postreplication type of recombinational repair, while single stranded DNA is interesting as a potential recombinogenic substrate.

Keywords: Escherichia coli, bacteriophages, plasmids, DNA recombination, DNA repair, DNA synthesis

Research goals: The aim of this project is to gain better insight into the regulations of DNA recombination and recombinational DNA repair. More specifically, we expect to find out what gene products are involved in two radiation induced processes one leading to the loss of prophage recombinogenicity and the other leading to the inhibition of multiplicity reactivation of irradiated phage. We will also attempt, in collaboration with other groups, to define more closely the role of postirradiation DNA synthesis in postreplication recombinational repair and the role of single stranded DNA synthesis in illegitimate recombination.

COOPERATION - PROJECTS


  1. Name of project: CRP/CRO93-01 Genes and enzymes involved in the inhibition of recombination
    Name of institution: International Centre for Genetic Engineering and Biotechnology (ICGEB)
    City: Trieste, Italia

COOPERATION - INSTITUTIONS


  1. Name of institution: Dr. S.D. Ehrlich, Institut National de la Recherche Agronomique
    Type of institution: University/Faculty
    Type of cooperation: Joint publishing of scientific papers
    City: Jouy-en-Josas, Francuska

  2. Name of institution: International Centre for Genetic Engineering and Biotechnology
    Type of institution: International organization
    Type of cooperation: Joint project
    City: Trieste, Italia
Other information about the project.
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