- Type of paper
: Paper in book
Title:
- Authors:
- Trgovčević, Željko (50435)
- Salaj-Šmic, Erika (42166)
- Editors
- Zergollern, Ljiljana
Publisher: Medicinska naklada
Year: 1994
Pages: from 69 to 78
Language: hrvatski
- Type of paper
: Paper in journal
Title: Tn5-rpsL: a new derivative of transposon Tn5 useful in
plasmid curing
- Authors:
- Stojiljković, Igor (126963)
- Trgovčević, Željko (50435)
- Salaj-Šmic, Erika (42166)
Journal: Gene
ISSN: 0378-1119
Volume: 99
Year: 1991
Pages: from 101 to 104
Number of references: 27
Language: engleski
Summary: The rpsL gene of Escherichia coli was inserted into the
BamHIsite of transposon Tn5. This transposon was called Tn5-rpsL.Tn5-rpsL
may be useful in microbiological studies when one wantsto cure variuos
bacterial genera of certain plasmid(s). Astreptomycin-resistant (SmR)
derivative of the host bacterialstrain is first isolated. The plasmid(s)
later to be cured arethen labelled with Tn5-rpsL, which makes the cells
SM-sensitive.These cells can regain their resistance to Sm if they lose
theTn5-rpsL-tagged plasmid. Thus, plasmid-free bacteria are selectedamong
SmR survivors. The freqency of occurrence of theplasmid-less variants of
plasmid-containing wild-type Salmonellatyphimurium measured by this method
is given as an example.
Keywords: Recombinant DNA, Escherichia coli, Salmonella typhimurium, streptomycin sensitivity
- Type of paper
: Paper in journal
Title: Interaction of RecBCD enzyme with DNA damaged by gamma
radation
- Authors:
- Brčić-Kostić, Krunoslav (157324)
- Salaj-Šmic, Erika (42166)
- Maršić, Nataša (172512)
- Kajić, Sanja
- Stojiljković, Igor (126963)
- Trgovčević, Željko (50435)
Journal: Mol. Gen. Genet.
ISSN: 0026-8925
Volume: 228
Year: 1991
Pages: from 136 to 142
Number of references: 39
Language: engleski
Summary: The DNA of a gene 2 mutant (T4 2-) of phage T4 is degraded
byRecBCD enzyme in bacterial cytoplasm. Under normal conditions,recBCD+
cells are therefore incapable of supporting the growth ofphage T4 2-. Only
if the nucleolytic activity of RecBCD enzyme isabsent from the cytoplasm
are T4 2--infected bacteria able toform plaques. We found that recBCD+
cells can form plaques if,before infection with T4 2-, they have been
exposed to gammaradiation. It is suggested that gamma ray-induced lesions
of thebacterial DNA (e.g., double-strand breaks) binds RecBCD enzyme.This
binding enables the enzyme to begin to degrade the bacterialchromosome, but
simultaneosly prevents its degradative action onthe ends of minor DNA
species, such as unprotecetd infectingphage chromosomes. Degradation of the
chromosomal DNA, whichoccurs during the early postirradiation period,
ceases about 60min after gamma ray exposure. The reappearance of the
nucleolyticaction of RecBCD enzyme on T4 2- DNA accompanies the cessation
ofdegradation of bacterial DNA. Both, this cessation and thereappearance of
the nucleolytic action of RecBCD enzyme on T4 2-DNA depend on a functional
recA gene product. These resultssuggest that postirradiation DNA
degradation is controlled by therecA-dependent removal from the damaged
chromosome. By making useof the temperature-sensitive mutant recB21, we
showed thatRecBCD-mediated repair of gamma ray-induced lesions occurs
duringthe early postirradiation period,i.e. during postirradation
DNAdegradation. It is shown that the RecD subunit of RecBCD enzymealso
participates in this repair.
Keywords: RecBCD enzyme, Gamma irradiation, DNA repair, recD gene, recA gene
- Type of paper
: Paper in journal
Title: Interaction of lambda Gam preotein with the RecD subunit
of RecBCD enzyme increases radioresistance of the wild-type Escherichia
coli
- Authors:
- Maršić, Nataša (172512)
- Salaj-Šmic, Erika (42166)
- Stojiljković, Igor (126963)
- Trgovčević, Željko (50435)
Journal: Biochimie
ISSN: 0300-9084
Volume: 73
Year: 1991
Pages: from 501 to 503
Number of references: 18
Language: engleski
Summary: By making use of the gam+-plasmid, the so-called
gam-dependentradioresistance was studied. This resistance is the result of
theinteraction between Gam protein (encoded by the gam gene oflambda) and
RecBCD enzyme of Escherichia coli. gam-dependentradioresistance is observed
in recB+recC+recD+ but not inrecB+recC+recD- cells. It is suggested that
Gam protein interactsspecifically with the RecD subunit of RecBCD enzyme;
the RecBCcomplex probably retains its activity in the presence of thisviral
protein.
Keywords: Lambda phage, Gam protein, Escherichia coli, RecBCD enzyme, gamma radiation
- Type of paper
: Paper in journal
Title: A possible interaction of single-strand binding protein
and RecA protein during post-ultraviolet DNA synthesis
- Authors:
- Trgovčević, Željko (50435)
- Petranović, Mirjana (36634)
- Brčić-Kostić, Krunoslav (157324)
- Petranović, Drago (36623)
- Lerš, Nella (123006)
- Salaj-Šmic, Erika (42166)
Journal: Biochimie
ISSN: 0300--908
Volume: 43
Year: 1991
Pages: from 515 to 517
Number of references: 27
Language: engleski
Summary: The mechanism of DNA replication in ultraviolet
(UV)-irradiatedEscherichia coli is proposed. Immediately after UVexposure,
thereplisome aided by single-strand DNA-binding protein (SSB) canproceed
past UV-induced pyrimidine dimers without insertion ofnucleotides.
Polimerisation eventualy resumes somewheredownstream of the dimer site. Due
to the limited supply of SSB, only a few dimers can be bypassed in this
way.Nevertheless, thisearly DNA synthesis is of great biological importance
because itgenerates single-stranded regions. Single-strans DNA can bind
andactivate RecA protein, thus leading to induction of the SOSresponse.
During the SOS response, the cellular level of RecAprotein increases
dramatically. Due to the simultaneous increasein the concentration of ATP,
RecA protein achieves thehigh-afinity state for single-stranded DNA.
Therefore it is ableto displace DNA-bound SSB. The cycling of SSB on and
off DNAenables the replisome to bypass a large number of dimers at
latepost-UV times. During this late replication, the stoichiometricamounts
of RecA protein needed for recombination are involved inthe process of
postreplication repair.
Keywords: Escherichia coli, UV radiation, post-UV DNA synthesis, SSB protein, RecA protein
- Type of paper
: Paper in journal
Title: The ability of rifampin-resistant Escherichia coli to
colonize the mouse intestine is enhanced by the presence of a
plasmid-encoded aerobactin-iron (III) uptake system
- Authors:
- Stojiljković, Igor (126963)
- Čobeljić, Miloje
- Trgovčević, Željko (50435)
- Salaj-Šmic, Erika (42166)
Journal: FEMS Microbiol. Lett.
ISSN: 0378-1097
Volume: 90
Year: 1991
Pages: from 89 to 94
Number of references: 27
Language: engleski
Summary: Rifampin-resistant Escherichia coli are known to be
poorcolonizers of the animal intestine. In this report, we show thatthe
colonizing ability of rifampin-resistant E.coli cells isincreased
dramatically in the presence of theaerobactin-mediated iron (III) uptake
system. In contrast, thecolonization by nalidixic acid-resistant E.coli
does neitherdepend on the aerobactin-iron (III) nor on the dicitrate
-iron(III) uptake system. Likewise, it does not depend on theproduction of
the siderophore enterochelin.
Keywords: Escherichia coli, iron transport, siderophore, aerobactin, rifampin resistance, colonization of mouse intestine
- Type of paper
: Paper in journal
Title: Iron and infection
- Authors:
- Stojiljković, Igor (126963)
- Salaj-Šmic, Erika (42166)
Journal: Liječ. Vjesn.
ISSN: 0024-3477
Volume: 113
Year: 1991
Pages: from 343 to 347
Number of references: 43
Language: hrvatski
Summary: Iron is essential nutrient for growth of most
pathogenicmicrooganisms. However, in vivo iron is complexed with
hostproteins such as transferrin in blood and lactoferrin insecretions so
that it is not available as a free ionic iron.Restriction in the
availability of free iron in the host , theso-called nutritional immunity,
plays a key role in nonspecificdefence strategy against potential
pathogens. To overcome thelack of free iron, microorganisms produce iron
chelatingsubstances called siderophores. The outcome of every infectionis
therefore dependent on both the level of free iron present inthe host and
the efficieny of siderophore-mediated iron uptakesystem of the pathogen.
Keywords: Iron, bacterial virulence, nutritional immunity, infection
- Type of paper
: Paper in journal
Title: The influence of mouse sera, regenerating liver extracts
and bacterial products on the abilities of different cells in vitro
- Authors:
- Žarković, Neven
- Osmak, Maja
- Novak, Đurđa
- Lerš, Nella (123006)
- Jurin, Mislav
Journal: Int. J. Dev. Biol.
Volume: 35
Year: 1991
Pages: from 239 to 249
Language: engleski
- Type of paper
: Paper in journal
Title: Overproduction of the RecD polypeptide sensitizes
Escherichia coli cells to gamma radiation
- Authors:
- Brčić-Kostić, Krunoslav (157324)
- Stojiljković, Igor (126963)
- Salaj-Šmic, Erika (42166)
- Trgovčević, Željko (50435)
Journal: Mutat. Res.
ISSN: 0921-8777
Volume: 281
Year: 1992
Pages: from 123 to 127
Number of references: 28
Language: engleski
Summary: We investigated DNA metabolism in Escherichia coli cells
carryingthe multicopy recD+ plasmid (pKI13). In the presence of pKI13,the
cellular level of the recD gene product (RecD polipeptyde)is amplified at
least 60-fold. Overproduction of the RecDpolypeptide has no effect on UV
repair and conjugalrecombination. In contrast, high cellular levels of
thispolypeptide sensitize wild-type cells to gamma radiation; also,they
increase the rate of radiation-induced DNA degradation. Apossible mechanism
for the enhancement of gamma-ray-inducedkilling by large amounts of the
RecD polypeptide is discussed.
Keywords: Escherichia coli, RecBCD enzyme, RecD gene, UV repair, gamma-ray repair
- Type of paper
: Paper in journal
Title: Hemin uptake system of Yersinia enterocolitica:
similarities with other TonB-dependent systems in Gram-negative bacteria
- Authors:
- Stojiljković, Igor (126963)
- Hantke, Klaus
Journal: EMBO J.
ISSN: 0261-4189
Volume: 11
Year: 1992
Pages: from 4359 to 4367
Number of references: 55
Language: engleski
Summary: The hemin receptor HemR of Yersinia enterocolitica was
identifiedas a 78 kDa iron regulated outer membrane protein. Cells devoidof
the HemR receptor as well as cells mutated in the tonB genewere unable to
take up hemin as an iron source. The hemin uptakeoperon from Y.
enterocolitica was cloned in Escherichia coli K12and was shown to encode
four proteins: HemP (6.5 kDa), HemR (78kDa), HemS (42kDa) and HemT (27
kDa). When expressed in E.colihemA aroB, a plasmid carrying genes for HemP
and HemR allowedgrowth on hemin as a porphyrin source. Presence of genes
forHemP, HemR and HemS was necessary to allow E.coli hemA aroB cellsto use
hemin as an iron source.The nucleotide sequence of thehemR gene and its
promoter region was determined and the aminoacid sequence of the HemR
receptor deduced. HemR has a signalpeptide of 28 amino acids and a typical
TonB box at itsamino-terminus. Upstream of the first gene in the operon
(hemP),a well conserved Fur box was found which is in accordance withthe
iron-regulated expression of HemR.
Keywords: Hemin, hemin uptake mutant, iron, TonB
- Type of paper
: Paper in journal
Title: In vivo studies on the interaction of RecBCD enzyme and
lambda Gam protein
- Authors:
- Maršić, Nataša (172512)
- Roje, Sanja
- Stojiljković, Igor (126963)
- Salaj-Šmic, Erika (42166)
- Trgovčević, Željko (50435)
Journal: J. Bacteriol.
ISSN: 0021-9193
Volume: 175
Year: 1993
Pages: from 4738 to 4743
Number of references: 45
Language: engleski
Summary: The interaction between the RecBCD enzyme of Escherichia
coli andthe lambda Gam protein was investigated. Two types of
experimentswere done. In one type, Gam protein was produced by
transientinduction of the cells lysogenic for lambda cI857 gam+.
Thepresence of Gam protein, which inhibits RecBCD nuclease, enabledthese
cells to support the growth of a gene 2 mutant ofbacteriophage T4 (T4 2).
The lysogens overproducing the RecBsubunit of RecBCD enzyme could titrate
Gam protein and thusprevent the growth of T4 2. In contrast, the
lysogensoverproducing either RecC or RecD retained their capacity forgrowth
of T4 2. It is therefore concluded that the RecB subunitis capable of
binding Gam protein. In the second type ofexperiments, Gam protein was
provided by depressing the gamS geneon the plasmid pSF117 (S.A. Friedman
and J.B. Hays, Gene43:255-263, 1986). The presence of this protein did not
interferewith the growth of wild-type cells (which were F-). Gam proteinhad
a certain effect on recF mutants, whose doubling time becamesignificantly
longer. This suggests that the recF gene productplays an important role in
maintenance of viability of theGam-expressing cells. Gam protein exerted
the most strikingeffect on growth of Hfr bacteria. In its presence, Hfr
bacteriagrew extremly slow, but their ability to transfer DNA torecipient
cells was not affected. We showed that the effect ongrowth of Hfr resulted
from the interaction between theRecBCD-Gam complex and the integrated F
plasmid.
- Type of paper
: Paper in journal
Title: Purification of yersiniabactin: a siderophore and possible
virulence factor of Yersinia enterocolitica
- Authors:
- Hantke, Klaus
- Drechsel, HARTMUNT
- Stojiljković, Igor (126963)
- Jung, Guenther
- Zaehner, Hans
Journal: J. Gen. Microbiol.
ISSN: 1350-0872
Volume: 139
Year: 1993
Pages: from 2159 to 2165
Number of references: 25
Language: engleski
Summary: HPLC analysis revealed that Yersinia enterocolitica WA-C
producedtwo substances under iron-limiting conditions one of which
wasidentified as 2,3-dihydrobenzoyl-L-serine. The other compound
hadiron-complexing activity and was called yersinia bactin. The furmutant
H1852 was shown to produce yersiniabactin constituvely inan
iron-independent manner. Yersiniabactin was isolated by ethylacetate
extraction from the spent medium of H1852,size-fractionation chromatography
and preparative HPLC. Acatechol function was demonstrated with different
chemical assaysand by UV-visible spectroscopy. The molecular mass
ofyersiniabactin was determined to be 482 Da. Purifiedyersiniabactin
stimulated growth of Y.enterocolitica andEscherichia coli fi under
iron-limiting conditions and apparentlyserved as an iron carrier. Transport
of 55Fe-yersiniabactin wasTonB-dependent, indicating a receptor-mediated
uptake across theouter membrane. A pesticin-resistant mutant missing the
receptorprotein FyuA was unable to transport and use yersiniabactin as
asiderophore.
- Type of paper
: Paper in journal
Title: Escherichia coli K-12 ferrous iron uptake mutants are
impaired in their ability to colonize the mouse intestine
- Authors:
- Stojiljković, Igor (126963)
- Čobeljić, Miloje
- Hantke, Klaus
Journal: FEMS Microbiol. Lett.
ISSN: 0378-1097
Volume: 08
Year: 1993
Pages: from 111 to 116
Language: engleski
- Type of paper
: Paper in journal
Title: Virulence of Yersinia enterocolitica is closly associated
with siderophore production, expression of an iron repressible outer
membrane polypeptide of 65000 daltons and pesticin-sensitivity
- Authors:
- Hessemann, J.
- Vocke, T.
- Hantke, Klaus
- Saken, E.
- Stojiljković, Igor (126963)
- Rakin, A.
- Berner, R.
Journal: Mol. Microbiol.
Volume: 8
Year: 1993
Pages: from 397 to 408
Language: engleski
- Type of paper
: Paper in journal
Title: Survey on newly characterized iron uptake systems of
Yersinia enterocolitica
- Authors:
- Baumler, A.
- Koebnik, I.
- Stojiljković, Igor (126963)
- Hessemann, J.
- Braun, V.
- Hantke, Klaus
Journal: Zbl. Mikrobiol.
ISSN: 0300-9688
Volume: 278
Year: 1993
Pages: from 416 to 424
Language: engleski
- Type of paper
: Paper in journal
Title: Control of gamma ray-induced DNA degradation in
Escherichia coli: a possible mechanism
- Authors:
- Salaj-Šmic, Erika (42166)
- Maršić, Nataša (172512)
- Trgovčević, Željko (50435)
Journal: Period. Biol.
Number: 4
ISSN: 0031-5362
Volume: 95
Year: 1993
Pages: from 447 to 449
Number of references: 9
Language: engleski
- Type of paper
: Paper in journal
Title:
- Type of paper
: Paper in journal
Title:
- Authors:
- Trgovčević, Željko (50435)
Journal: Liječ. Vjesn.
ISSN: 0024-3477
Volume: 116
Year: 1994
Pages: from 315 to 318
Language: hrvatski
- Type of paper
: Paper in journal
Title:
Journal: Zbl. Mikrobiol.
- Type of paper
: Paper in journal
Title:
- Authors:
- Baumler, A.
- Type of paper
: Paper in journal
Title:
- Type of paper
: Summary in proceedings
Title: Isolation and characterization of temperature-senzitive
recD mutants of Escherichia coli
- Authors:
- Lerš, Nella (123006)
- Salaj-Šmic, Erika (42166)
- Trgovčević, Željko (50435)
Proceedings title: Godišnji sastanak hrvatskih biokemičara
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 112 to 112
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
Summary: To gain a better insight into the function of the RecBCD
enzyme,we isolated temperature-sensitive recD mutants. To achieve this,we
took advantage of the fact that recD mutants produce many moreplasmid
copies than the recD+ bacterial counterpart. In the firststage of
selection, wild-type DM456 cells bearing the CmRmini-F(pLCF2) plasmid were
UV mutagenized and then seedes onplates with high concentrations of
chloramphenicol. The plateswere incubated at 42oC. In the second
stage,chloramphenicol-resistant colonies were examined for the phage T42
growth.(T4 2 can grow in the absence of the recD gene productbut not in its
presence.). Candidates for thetemperature-sensitive recD mutants were those
bacteria that weresensitive to T4 2 at 40oC. By complementation analysis,
it turnedout that 2 out 38 colonies carried the
temperature-sensitivemutation in the recD gene.
- Type of paper
: Summary in proceedings
Title: The interaction of lambda Gam protein with the individual
subunits of RecBCD enzyme of Escherichia coli
- Authors:
- Maršić, Nataša (172512)
- Salaj-Šmic, Erika (42166)
- Trgovčević, Željko (50435)
Proceedings title: Godišnji sastanak hrvatskih biokemičara
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 114 to 114
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
Summary: It is well known that Gam protein, the product of the gam
gene ofphage lambda, can modify the RecBCD enzyme of E. coli. RecBCDenzyme
consists of three subunits encoded by the recB, recC andrecD genes. Since
Gam-expressing bacteria are at leastsuperficially similar to bacteria
carrying the recD mutation, thequestion arises whether Gam protein binds to
the RecD subunit orwhether it interacts with some other components of the
RecBCDenzyme in vivo. To answer this question a simple genetic approachwas
used. Gam protein was produced by transient induction of thebacteria
lysogenic for lambdacI857gam+. The presence of Gamprotein, which inhibits
RecBCD nuclease, enabled these cells tosupport the growth of a gene 2-
mutant of phage T4. T4 2 platesat high efficiency on the pulse-heated gam+
lysogens. Thisincreased plating efficiency of T4 phage may be less
pronouncedor even eliminated if the cells overproduce the component
ofRecBCD enzyme that exerts the "titration" effect on Gam protein.Our
results show that the lysogen overproducing RecB subunit cantitrate Gam
protein and thus prevent the growth of T4 2. It isconcluded that Gam
protein binds to RecB subunit of RecBCDenzyme.
- Type of paper
: Summary in proceedings
Title: The role of RecD enzyme in DNA transfer during
Hfr-mediated bacterial conjugation
- Authors:
- Džidić, Senka (40026)
- Maršić, Nataša (172512)
- Trgovčević, Željko (50435)
Proceedings title: Godišnji sastanak hrvatskih biokemičara
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 113 to 113
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
Summary: RecBCD enzyme of Escherichia coli unwinds DNA from duplex
DNAends to produce single stranded DNA, a central intermediate inthe major
pathway of homologous recombination. There is abiochemical evidence that
RecB subunit of the RecBCD complexbinds to 3'terminated strand and acts as
ATP-dependent singlestranded translocase. RecCD subunit binds to
5'terminated strandand unwinds DNA acting as helicase (see Ganesen and
Smith,J.Mol.Biol. 229,1993,67-78). We have used genetic approach totest
this hypothesis. Two types of experiments were carried out.First, we used
HfrH cells expressing the lambda gam geneproduct,which binds RecB subunit
of the RecBCD enzyme. Duringconjugation between these Hfr cells and the
wild-type F- strains,the normal yields of recombinants was obtained.
Second, we usedHfr cells carrying the recD mutation. In crosses between
thesecells and the F- strain,the yield of recombinants was
graetlyreduced.Evidently, conjugal DNA transfer is impaired in theabsence
of the functional RecD subunit. Thus, our genetic datasupport the
hypothesis that RecCD subunit of RecBCD enzyme actsas a helicase.
- Type of paper
: Summary in proceedings
Title: Inhibition of restriction of the unmodified DNA by linear
multimers
- Authors:
- Salaj-Šmic, Erika (42166)
- Maršić, Nataša (172512)
- Trgovčević, Željko (50435)
Proceedings title: Godišnji sastanak hrvatskih biokemičara
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 115 to 115
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
Summary: Plaque formation by phage lambda grown on Escherichia coli
C is restrictedby several orders of magnitude when plated on E. coliK-12.
This restriction acts on the unmodofied phage DNA throughthe cleavage by
restriction endonuclease EcoK and subseqentexonucleolytic degradation by
RecBCD enzyme. A partial realase oflambda DNA restriction is observed when
the E.coli host ispreirradiated with UV light. This phenomenon has been
calledUV-induced restriction alleviation. We are first to show that
thepresence of plasmids can also lead to the alleviation ofrestriction.
This happens in RecB and RecD strains. The presenceof the lambda gam gene
on the plasmid also alleviates therestriction in the wild-type and sbcC+
strains.This phenomenonwas not obtained with the recA and sbcE strain. As
shown by ourDNA hybridization experiments, restriction alleviation, in
allcases tested, is associated with the formation of linear
plasmidmultimers. We suggested that EcoK cleaves linear multimers
but,subsequent to the clevage, it may not turn over as a nuclease,thus
allowing lambda to begin its develompent.
- Type of paper
: Summary in proceedings
Title: The interaction of lambda Gam protein with the individual
subunits of RecBCD enzyme of Escherichia coli
- Authors:
- Salaj-Šmic, Erika (42166)
- Maršić, Nataša (172512)
- Trgovčević, Željko (50435)
Proceedings title: Cell cycle chechpoints, DNA repair and DNA replication strategies
Language: engleski
Place: Cambridge, Engleska
Year: 1993
Pages: from 158 to 158
Meeting: Cell cycle checkpoints, DNA repair and DNA replication strategies
Held: from 09/27/93 to 09/01/93
Summary: It is well known that Gam protein, the product of the gam
gene ofphage lambda, can modify the RecBCD enzyme of E. coli. RecBCDenzyme
consists of three subunits encoded by the RecB, recC andrecD genes. Since
Gam-expressing bacteria are at leastsuperficially similar to bacteria
carrying the recD mutation, thequestion arises whether lambda Gam protein
binds to the RecDsubunit or whether it interacts with some other components
of theRecBCD enzyme in vivo. To answer this question a simple
geneticapproach was used. Gam protein was produced by transientinduction of
the bacteria lysogenic for lambda cI857gam+. Thepresence of Gam protein,
which inhibits RecBCD nuclease, enabledthese cells to support the growth of
a gene 2 mutant of phage T4.T42 plates at high efficiency on the
pulse-heated lambda gam+lysogens. This increased plating efficiency of T4 2
may be lesspronounced or even eliminated if the cells overproduced
thecomponent of RecBCD enzyme that exerts the "titration" effect onGam
protein. Our results show that the lysogen overproducing RecBsubunit can
titrate Gam protein and thus prevent the growth of T42. It is concluded
that Gam protein binds to the RecB subunit ofRecBCD enzyme.
- Type of paper
: Summary in proceedings
Title: In vivo modulation of EcoK restriction endonuclease
- Authors:
- Salaj-Šmic, Erika (42166)
- Donjerković, Dubravka
- Maršić, Nataša (172512)
- Trgovčević, Željko (50435)
- Editors
- Gomerčić, Hrvoje
Proceedings title: Peti kongres biologa Hrvatske
Language: hrvatski
Place: Zagreb
Year: 1994
Pages: from 74 to 75
Meeting: Peti kongres biologa Hrvatske
Held: from 10/03/94 to 10/07/94
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Trgovčević, Željko (50435)
Language: engleski
Year: 1994
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 10/14/94 to 10/15/94
- Type of paper
: Ph.D.
Title:
- Type of paper
: Mentorship
Title: Influence of the lambda Gam protein on the physiology of
the host bacterium
Faculty: Prirodoslovno-matematički Zagreb
Mentor: TRGOVČEVIĆ ŽELJKO
Date of defense: 07/08/93
Number of pages: 112
Author: Maršić dr. Nataša
Degree level: Ph.D.
- Type of paper
: Mentorship
Title:
Faculty: Prirodoslovno-matematički Zagreb
Mentor: SALAJ-ŠMIC ERIKA
Date of defense: 11/15/94
Number of pages: 70
Author: Čogelja dipl.inž. Gordana
Degree level: D.A.
- Type of paper
: Mentorship
Title:
Faculty: Prirodoslovno-matematički Zagreb
Mentor: SALAJ-ŠMIC ERIKA
Date of defense: 07/05/94
Number of pages: 70
Author: Čogelja dipl.inž. Gordana
Degree level: D.A.
- Type of paper
: Mentorship
Title:
Faculty: Prirodoslovno-matematički Zagreb
Mentor: TRGOVČEVIĆ ŽELJKO
Date of defense: 06/29/94
Number of pages: 50
Author: Đermić Damir
Degree level: D.A.
- Type of paper
: Invited lecture
Title:
- Type of paper
: Invited lecture
Title:
- Type of paper
: Invited lecture
Title:
Institution: Godišnji sastanak hrvatskih biokemičara
Year: 1994
