SVIBOR - Project code: 1-08-217

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

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Project code: 1-08-217


THE ROLE OF RECOMBINATION IN DNA REPAIR AND PLASMID STABILITY


Main researcher: TRGOVČEVIĆ, ŽELJKO (50435)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/93.
Papers on project (total): 45
Papers on project quoted in Current Contents: 22
Institution name: Institut "Ruđer Bošković", Zagreb (98)
Department/Institute: Department of Molecular Genetics Laboratory for Microbial Genetics
Address: Bijenička 54
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 01 4561099
Fax: 385 01 425 497
E-mail: erika.salaj@400.irb.hr.
Summary: By the use of biochemical and genetic methods, the role of RecBCD enzyme in the bacterial growth, plasmid maintenance, DNA repair and Hfr-mediated recombination will be studied. The enzyme's RecD subunit, whose function in these processes is not yet understood, will receive our special attention. The interaction of RecBCD enzyme with bacterial and phage proteins (RecA, lambda Gam) will also be included in our study.

Keywords: DNA recombination, DNA repair, E. coli, RecBCD enzyme, (lambda) Gam protein

Research goals: Simple biological systems are indispensable to the studies of DNA transactions.The molecular mechanisms of geneticrecombination are poorly understood even in these systems.In an attempt togain a better understanding of the recombination processes, the Escherichia coli bacterium,the coliphage lambda and the various types of plasmids will be used. Our aim is toanswer the following questions.(i) What is the role of the bacterial recombination system in DNA repair? (ii) What is therole of the bacterial recombination system in the maintenance ofplasmids?The main interest will be focused on RecBCD enzyme which participates in homologous recombination in E.coli. This enzymeis very complex; it consists of three subunits (RecB, RecC andRecD). We plan to study the interaction of RecBCD enzyme with damaged DNA,with Gam protein (encoded by the gam gene of lambda)and with some bacterial proteins (which are of importance for plasmid maintenance). The role of the enzyme'sindividual subunits in these processes will also be studied.Furthermore,our research will be extended to the field of medical microbiology.The plasmids that confer certain pathogenic traits to enterobacteria will receive our special attention. Weintend to develop a method which would enable us to eliminatesuch plasmids from the bacteria.
Other information about the project.
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