- Type of paper
: Paper in journal
Title: Regeneration and reproduction of irradiated green hydra
(Cnidaria)
- Authors:
- Žnidarić, Danica (55795)
- Lui, Ante (103943)
- Kalafatić, Mirjana
Journal: Periodicum Biologorum
Number: 2
ISSN: 0031-5362
Volume: 94
Year: 1992
Pages: from 99 to 104
Number of references: 8
Language: engleski
Summary: Green hydras (Hydra viridissima), which contain symbiotic
algae of the genus Chlorella in their digestic cells, when exposed to
UV-light (253.5 um, 1.2 J/sm2) for 2, 3 and 5 minutes from a 1 m distance
become damaged and shrunk, whereas those exposed for 5 minutes die. The
surface mucous layer and much of the hypostome was damaged. During
regeneration of surviving individuals these parts recover rapidly. In this,
as in many previous experiments, zymogen cells had the ability to
dedifferentiate intogastrodermal interstitial cells and to diffrentiate
into mucous cells. During the regeneration all these stages could be seen
on histological preparations. Changes of zymogen cells were equal to those
in the regeneration and in the formation of new buds during the vegetative
reproduction. In this work it has been ascertained that UV-irradiation
increased the number of buds on green hydra, especially in groups
irradiated for 2 or 3 minutes. In periods after exposure, it seems that the
number and the volume of algae increased in the gastrodermal cells.
Keywords: Green hydra, Chlorella, UV-irradiation, regeneration, zymogen cells, budding
- Type of paper
: Paper in journal
Title: In memoriam
- Authors:
- Kalafatić, Mirjana
- Pavičić, Vicko (81862)
- Žnidarić, Danica (55795)
Journal: Natura Croatica
Number: 1
ISSN: 1330-0520
Volume: 2
Year: 1993
Pages: from 99 to 103
Language: hrvatski
- Type of paper
: Paper in journal
Title: The effect of insecticides upon green hydra
- Authors:
- Kalafatić, Mirjana
- Žnidarić, Danica (55795)
- Kopjar, Nevenka (195102)
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 129 to 130
Number of references: 5
Language: engleski
- Type of paper
: Paper in journal
Title: Response of Green Hydra to the Treatment with Different
Pesticides under Laboratory Conditions
- Authors:
- Kalafatić, Mirjana
- Kopjar, Nevenka (195102)
Journal: Zeitschrift fuer angewandte Zoologie
Number: 2
ISSN: 0044-2291
Volume: 80
Year: 1994
Pages: from 213 to 223
Number of references: 6
Language: engleski
Summary: The three insecticides used in the experiments caused
various changes to green hydras and algae which live as endosymbionts
inside the myoepithelial gastrodermal cells. Treated hydras were
contracted, with marked damage to the anterior part of the body. The
tentacles were damaged totheir very base. The outer mucous layer was peeled
along the entire animal. In these places the membranes of myoepithelial
cells were also damaged. The gastrodermal cavity contained a lot of free
hyaline cells. The zymogen cells changed their shape and position. Green
algae were situated along the myoepithelial cells, and not in the basal
part as in the control animals. All three insecticides used caused
stimulation of the asexual reproduction of the treated green hydras.
Keywords: Green hydra, insecticides, treatment, cytohistological, regeneration, differentiation, dedifferentiation, budding
- Type of paper
: Paper in journal
Title: The effect of pesticides on hydra
- Authors:
- Kalafatić, Mirjana
Journal: Priroda
Number: 772
ISSN: 0351-0662
Volume: 80
Year: 1991
Pages: from 16 to 16
Language: hrvatski
- Type of paper
: Paper in journal
Title: How did dinosaurs run
- Authors:
- Kalafatić, Mirjana
- Kružić, Petar
Journal: Priroda
Number: 807
ISSN: 0351-0662
Volume: 84
Year: 1994
Pages: from 27 to 29
Language: hrvatski
- Type of paper
: Paper in journal
Title: Symbiosis
- Authors:
- Kalafatić, Mirjana
- Kopjar, Nevenka (195102)
Journal: Priroda
Number: 812
ISSN: 0351-0662
Volume: 85
Year: 1995
Pages: from 12 to 13
Language: hrvatski
- Type of paper
: Paper in journal
Title: RNA - praemolecule of life
- Authors:
- Kopjar, Nevenka (195102)
- Kalafatić, Mirjana
Journal: Priroda
Number: 812
ISSN: 0351-0662
Volume: 85
Year: 1995
Pages: from 12 to 13
Language: hrvatski
- Type of paper
: Summary in proceedings
Title: Regeneration of the hypostome and the foot of hydra
treated with insecticide
- Authors:
- Barčot, Marina
- Žnidarić, Danica (55795)
- Lui, Ante (103943)
- Editors
- Šerman, Draško
Proceedings title: Zbornik priopćenja četvrtog kongresa biologa Hrvatske
Language: hrvatski
Place: Zagreb
Year: 1993
ISBN/ISSN: 953-6241-00-5
Pages: from 141 to 142
Meeting: Četvrti kongres biologa Hrvatske
Summary: The species of Hydra vulgaris Pallas was cut in the area of
the gastral region and treated with Dimiline WP 25 (Galenika-Beograd), with
Torak EC 24 and with Gamacide 20 (Pliva-Zagreb) respectively. The results
show that amputated parts of the body regenerate within three days after
the beginning of the experiment, although, the first day, compared to
control, inhibition of the foot regneration was noticed. The changes are
most evidently seen immediately after the treatment. All the parts are
shrunk. The hydras treated with Gamacide 20 show the highest mortality
rate. 20% of the treated hypostomal parts (H-part) died. Pedal parts
(P-part) are considerably resistant to the insecticides used. The
hypostomes of the hydras treated with Gamacide 20 were damaged in 40-5
cases. The wounds of all treated hydras heal first with the gastroderm and
the ectoderm forms afterwards. In the course of regeneration of amputated
parts of the body a significant part was played by zimogen cells. By their
dedifferentiation and differentiation, they replace cells that form
amputated parts of the body. Along with regeneration, hydras treated with
Dimiline WP 25 and Torak EC 24 were budding equally as the control while
the hydras treated with Gamacide 20 were not.
- Type of paper
: Summary in proceedings
Title: Regeneration and reproduction of irradiated green hydra
(Cnidaria)
- Authors:
- Žnidarić, Danica (55795)
- Lui, Ante (103943)
- Barčot, Marina
- Editors
- Šerman, Draško
Proceedings title: Zbornik priopćenja Četvrtog kongresa biologa Hrvatske
Language: hrvatski
Place: Zagreb
Year: 1993
ISBN/ISSN: 953-6241-00-5
Pages: from 140 to 141
Meeting: Četvrti kongres biologa Hrvatske
Summary: Green hydras (Hydra viridissima) whic contain symbiotic
algae of the Chlorella genus in their digestive cells, when exposed to UV
light (2535 um, 1.2 JS-1m-2) for 2, 3 and 5 minutes from a 1m distance
become damaged and shrunk, while some of those treated for 5 minutes die.
The surface ectodermal layer and many of the hypostomes are ruined. In the
course of regeneration of the surviving individuals these parts recover
rapidly. In this study as in many previous examinations, zymogen cells are
found to have the abilitz to dedifferentiate into gastrodermal interstitial
cells and differentiate into mucous cells. During the regeneration all
these stages can be seen on histological preparations. It is interesting to
state that changes of zymogen cells equal those in the regeneration and in
the formation of new buds during the vegetative reproduction. In this work
it has been ascertained that UV irradiation considerablz increases the
number of buds in green hydra, especially in the groups irradiated for 2
and 3 minutes. Besides, in periods after exposure, the number and the
volume of algae increase.
- Type of paper
: Summary in proceedings
Title: Karyological analysis of two populations of the Polycelis
felina species
- Authors:
- Kopjar, Nevenka (195102)
- Jelenić, Srećko
- Kalafatić, Mirjana
- Ladušić-Hanzir, Gordana
- Editors
- Gomerčić, Hrvoje
Proceedings title: Zbornik sažetaka priopćenja
Language: hrvatski
Place: Zagreb
Year: 1994
ISBN/ISSN: 953-6241-01-3
Pages: from 99 to 100
Meeting: Peti Kongres biologa Hrvatske
Held: from 10/03/94 to 10/07/94
Summary: Two geographicallz isolated populations of the planarian
Polycelis felina, sampled from habitats fish pond Gračani and spring of
Lonja, were cztologically analysed. In the purpose of inducing cell
divisions, animals were cut in four to five pieces. On the third day after
the regeneration started, regenerating segments were treated with
colhicine, which stopped mitoses in metaphase. Cytological preparations
were made, using the squash technique. The slides were frozen in liquid CO2
and stained with lacto-orceine or fluorochromes. Chromosomes from the
neoblasts, intensivelz divided cells, were analysed. Karyological analyses
demonstrated diploidy and equal number of chromosomes (2n=2X=18) in both
population investigated. The results show that both of investigated
planarian populations, despite of their geographic isolation, belong to the
same karyological biotype.
- Type of paper
: Summary in proceedings
Title: The surviving of hydra (Hydra oligactis) in inadequate
life conditions
- Authors:
- Žnidarić, Danica (55795)
- Kalafatić, Mirjana
- Kopjar, Nevenka (195102)
- Editors
- Gomerčić, Hrvoje
Proceedings title: Zbornik sažetaka priopćenja
Language: hrvatski
Place: Zagreb
Year: 1994
ISBN/ISSN: 953-6241-01-3
Pages: from 355 to 356
Meeting: Peti Kongres biologa Hrvatske
Held: from 10/03/94 to 10/07/94
Summary: The hydras treated with insecticides or UV irradiation
react similar to both of these factors. Immediately after treatment, the
animals contract and take the smallest possible space, so the penetration
of factors into interior part of the body is greatly reduced. Therefore,
the organism will in short period survive inadequate life conditions, while
longer treatment and higher quantities of factors will destroy it. It is
interest to observe the other possibilities of surviving of these animals.
Namely, the hydras have the capability to "eject" highly differentiated
cells (cnidas), damaged and dead cells, through hypostome, out of their
bodies into surrounding water. The rest of remained cells are the young
populations of nondifferentiated and little differentiated cells, which
concentrated in budding region, where by processes of differentiation and
morphogenesis they create a higher number of buds, than the control
animals. Increased number of descendents gives the Hydra oligactis greater
possibility of surviving in inadequate conditions.
- Type of paper
: Summary in proceedings
Title: The effect of pesticides upon the symbiosis of hydra and
algae
- Authors:
- Kalafatić, Mirjana
- Kopjar, Nevenka (195102)
- Žnidarić, Danica (55795)
- Petrin, Marina
- Editors
- Gomerčić, Hrvoje
Proceedings title: Zbornik sažetaka priopćenja
Language: hrvatski
Place: Zagreb
Year: 1994
ISBN/ISSN: 953-6241-01-3
Pages: from 356 to 357
Meeting: Peti Kongres biologa Hrvatske
Held: from 10/03/94 to 10/07/94
Summary: The green hydra (Hydra viridissima Pallas) is a typica
example of endosymbiosis. Inside its myoepithelial cells symbiotically
lives the population of unicelular green algae from the Chlorella genus. It
is known that symbiotic green hydras, as well as nonsymbiotic species of
hydra, have a great ability of regeneration. Strong polution diminishes the
surviving capacity of these organisms. Immediately after the treatment with
8, respectively 10 mg/l of Torak EC 24, 500, respectively 600 mg/l of
Dimiline WP 25 and 10, respectively 20 mg/l of Gamacide 20, the reduction
of interstitial cells and their derivatives from ectoderm is noticed. The
cnidas and cnidoblasts in gastroderm are present in greater number. Among
the gastrodermal zymogen cells stronger transdifferentiation was noticed.
The membranes of ectodermal and gastrodermal myoepithelial cells were
damaged. Inside the gastrodermal myoepithelial cells the cytoskeleton was
damaged, so immediately after tha treatment the green algae were spread
along the entire cell. Four days after treatment they occupy their normal
position in those parts of the myoepithelial cells where the intracellular
digestion is weaker. By means of electron microscope it was established
that algal ultrastructure was not damaged after treatments. Some of used
pesticides in lower concentrations had the hormestic effect upon the
asexual reproduction. So hydras treated with 500, respectively 600 mg/l of
Dimiline WP 25 budded twice as much as the control ones.
- Type of paper
: Summary in proceedings
Title: The effect of insecticide upon the green hydra
- Authors:
- Kalafatić, Mirjana
- Žnidarić, Danica (55795)
- Kopjar, Nevenka (195102)
Proceedings title: Abstracts 16th Alps-Adria Meeting of Anatomists
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 87 to 87
Meeting: 16th Alps Adria Meeting of Anatomists
Held: from 05/20/93 to 05/23/93
- Type of paper
: M.A.
Title: The effect of heavy metals upon the cell division in
regenerates of Polycelis felina species
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Author: KOPJAR NEVENKA
Date of defense: 04/26/95
Language: hrvatski
Number of pages: 103
Summary: In this work morphological, histological and cytological
constitution of Polycelis felina species during 96 hours of regeneration
was investigated. Furthermore, karyotype of this species was analised as
well as mitotic activity of neoblasts, the only mitotic active cell type of
these animals. Then, the effects of three heavy metals mercury, cadmium and
manganese upon the genetic material and mitosis of neoblasts were
investigated. The results of these experiments indicate that all three
heavy metals are cytotoxic for the Polycelis felina species. In low
concentrations (1-10 um HgCl2, 1-100 um 3CdSO4x8H2O and 10-1000 um
MnCl2x4H2O) they caused mortality of certain number of treated animals, and
in survived ones they caused a decrease in mitotic activity of neoblasts
and different mitotic and chromosomal aberrations. Mitotic aberrations,
such as C-mitosis, and chromosomal aberrations, such as sticky chromosomes,
indicate that the effects of these metals are based upon changes of protein
componente. The results of investigation of the effects of heavy metals
upon the Polycelis felina species are in correlation with the results
obtained in other test systems, and justified the use of this animal in
short term in vivo cytogenetical test for the detection of genotoxicity of
chemical compounds.
Keywords: Polycelis felina, regeneration, neoblasts, mitotic index, chromosomes, karyotype, heavy metals, chromosomal aberrations, mitotic aberrations.
- Type of paper
: Mentorship
Title: The effect of heavy metals upon the cell division in the
regenerates of Polycelis felina species
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KALAFATIĆ MIRJANA
Date of defense: 04/26/95
Number of pages: 103
Author: Kopjar diplomirani inženjer biologije Nevenka
Degree level: M.A.
- Type of paper
: Mentorship
Title: Interaction of the UV irradiation and lindane upon the
changes of celular structure of hydra (Hydra viridissima Pallas)
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KALAFATIĆ MIRJANA
Date of defense: 05/19/92
Number of pages: 41
Author: Kopjar diplomirani inženjer biologije Nevenka
Degree level: D.A.
- Type of paper
: Mentorship
Title: Regeneration of the damaged body parts and asexual
reproduction of the hydra (Hydra viridissima Pallas) after the treatment
with Gamacide 20
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KALAFATIĆ MIRJANA
Date of defense: 10/22/92
Number of pages: 46
Author: Barčot student biologije Marina
Degree level: D.A.
- Type of paper
: Mentorship
Title: Effect of Gamacide 20 upon the regeneration of tentacles
and cztological structure of green hydra (Hydra viridissima Pallas)
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KALAFATIĆ MIRJANA
Date of defense: 10/22/92
Number of pages: 45
Author: Milić student biologije Đurđa
Degree level: D.A.
- Type of paper
: Mentorship
Title: Effect of pirimicarb upon green hydra (Hydra viridissima
Pallas)
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KALAFATIĆ MIRJANA
Number of pages: 28
Author: Kutela student biologije Nataša
Degree level: D.A.
- Type of paper
: Mentorship
Title: Effect of atrazine upon green hydra
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KALAFATIĆ MIRJANA
Author: Arapović student biologije Tatjana
Degree level: D.A.
- Type of paper
: Mentorship
Title: Budding of green hydra and division of the endosymbiotic
alga in dark
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KALAFATIĆ MIRJANA
Author: Palarić student biologije Nela
Degree level: D.A.
- Type of paper
: Mentorship
Title: Karyological investigation of two populations of species
Polycelis felina (Turbellaria)
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KALAFATIĆ MIRJANA
Date of defense: 09/25/95
Number of pages: 35
Author: Ladušić-Hanzir student biologije Gordana
Degree level: D.A.
- Type of paper
: Mentorship
Title: Regeneration and budding of hydras (Hydra viridissima
Pallas) irradiated with UV light
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: ŽNIDARIĆ DANICA
Date of defense: 09/23/93
Number of pages: 35
Author: Vađon student biologije Nataša
Degree level: D.A.
- Type of paper
: TV broadcast
Title:
- Type of paper
: Manuscript
Title: The effect of the manganese on the cell division in
planarian Polycelis felina
- Authors:
- Kopjar, Nevenka (195102)
- Kalafatić, Mirjana
- Besendorfer, Višnja
Language: engleski
