SVIBOR - Project code: 1-08-263

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
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E-mail: ured@znanost.hr

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Project code: 1-08-263


INFLUENCE OF HORMONES ON NEONATAL ADRENALS ACTIVITY


Main researcher: KLEPAC, RATIMIR (21205)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/96.
Papers on project (total): 5
Institution name: Medicinski fakultet, Zagreb (108)
Department/Institute: Department of biology
Address: Šalata 3
City: 10000 - Zagreb, Croatia
Communication
Summary: Mammlian adrenal glands are sensitive on higher amount of oxygen(hyperoxia).Molecular oxygen easily transformed in agresive oxygen radicals which damaged different tissues.In this project we have investigated influence of high amount of oxygen on level of vitamin C,cytochrome P-450 and lipid peroxidation in adrenal cortex mitochondria and microsomes.Adrenal organelles were bubbled with oxygen 5-90 min at 37 degres.Oxygen strongly deplited level of vitamin C and cytochrome P-450 and increased lipid peroxidation in adrenal cortex mitochondria and microsomes. Adrenal mitochondria incubated with different amount of vitamin C synthetized in vitro higher amount of steroid hormones than intact adrenals.These results show that vitamin C is important antioxidant which protects adrenals from harmful activity of free oxygen radicals.

Keywords: Adrenal glands,vitamin C,cytochrome P-450,steroid synthesis, lipid peroxidation.

Research goals: We have desired to determine reactivity of adrenal mitochondria and microsomes on higher amount of oxygen (hxperoxia).It is known that molecular oxygen formed reactive free radicals which damaged all lipids.proteins.DNA and cell function.In this investigation we wished to found activity on enzymes of steroid hormone hydrxilation in adrenal cortex after hyperoxia.

COOPERATION - PROJECTS



COOPERATION - INSTITUTIONS


  1. Name of institution: NOVA BOLNICA DUBRAVA
    Type of institution: Institution whose primary activities ar
    Type of cooperation: Joint publishing of scientific papers
    City: 10000 - Zagreb, Croatia
Other information about the project.
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