SVIBOR - Project code: 1-08-268

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

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Project code: 1-08-268


Regeneration and transformation in plant cells


Main researcher: JELASKA, SIBILA (18296)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/95.
Papers on project (total): 82
Papers on project quoted in Current Contents: 8
Institution name: Prirodoslovno-matematički fakultet, Prirodoslovni odjeli, Zagreb (119)
Department/Institute: BIOLOGICAL DIVISION DEPARTMENT OF MOLECULAR BIOLOGY
Address: Rooseveltov trg 6
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 4552-646
Phone: 385 (0)1 442-804
Fax: 385 (0)1 4552-645
Phone: 385 (0)1 442-604
Phone: 385 (0)1 412-912
Summary: In control condition of in vitro culture the differentiation and regeneration of plant organisms will be investigated (Cucurbita pepo, horse radish and others). Gene activity and nuclear genome in determined phases of growth and development will be observed. Biochemical and gene manipulation with using Ti and Ri plasmids will be tested.

Keywords: Regeneration, somatic embryogenesis, transformation, regulation of gene expression during regeneration and transformation, morphogenesis, differentiation, dedifferentiation, organogenesis, habituated tissues and cells, transgenic plants, modification of secondary metabolite production in cell cultures.

Research goals: Goal Establishment the role of endogenic and exogenic factors (plant growth regulators, physical conditions, genom modification) in physiological and genetical regulation of organogenesis and somatic embryogenesis in plant cell and tissue culture in vitro. Result - Conditions for the establishment, multiplication and adventitions root induction of juvenile Quercus robur plants; for establishement of whild cherry primary culture, multiple shoot induction and plant regeneration in Betula pendula, the effect of genotype on Gerbera shoot multiplication, influence of meristem culture and virus elimination on phenotypical modifications of grapevine (V. vinifera L. cv. Refošk) were found. - Peroxidases were studied in plant species in relation to differentiation process and tumor transformation. Distinctive markers of morphogenesis and tumori genesis were found. In habituated pumpkin callus lines an increase in peroxidase activity meant that the embryogenesis was triggered. Crown-gall and hairy roots were produced in horse-radish and pumpkin explants. Parameters for pumpkin embryo maturation and unlimited production of normal plants from very long-term embryogenic cultures were analysed.

COOPERATION - PROJECTS


  1. Name of project: 1-08-326 Ekspresija gena u tijeku razvoja viših biljaka
    Name of institution: Institut Ruđer Bošković, Centar za molekularnu biologiju
    City: 10000 - Zagreb, Croatia

  2. Name of project: Etude de l'Embryogenese Somatique et de l'Organogenese chez les Coniferes
    Name of institution: Universite Claude Bernard - Lyon 1, 69622 Villeurbanne Cedex, France
    City: Villeurbanne - Cedex, France

COOPERATION - INSTITUTIONS


  1. Name of institution: Institut Ruđer Bošković, Centar za molekularnu biologiju
    Type of institution: University/Faculty
    City: 10000 - Zagreb, Croatia

  2. Name of institution: Universite Claude Bernard - Lyon 1
    Type of institution: Economical/Production
    City: Villeurbanne - Cedex, France
Other information about the project.
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