- Type of paper
: Paper in journal
Title: Immunohistochemical and stereological study of
neuroendocrine cells in human antrum during the perinatal period
- Authors:
- Rode, Bojan (41266)
- Lacković-Venturin, Gordana (105166)
- Pirkić, Ahmed (11026)
Journal: International Journal of Developmental Biology
Number: 3
ISSN: 0214-6282
Volume: 35
Year: 1991
Pages: from 303 to 309
Language: engleski
Summary: A correlative immunohistochemical and stereological study
ofneuroendocrine cells (NEC) was carried out in the antrum oftwenty human
fetuses with gestational ages from 18 to 42 weeksand of two specimens
postnatally. Neuron-specific enolase (NSE)as a common marker of neurons and
NEC, as well as gastrin (G-)and somatostatin (D-) immunoreactive cells
served for evaluationof volume density, which proved to be the most
convenient methodfor quantitative analysis of NEC. It was observed that
aconsiderable frequency of NEC appeared at 23-24 weeks ofgestation (8% of
NSE and 6% of G-cells) and coincided with theadult pattern of intramural
innervation. After a repeatedincrease of NEC in the 26-week-old fetus, the
frequency of NECremained persistant during the perinatal period (10-12% of
NSEand 7-8% of G-cells). An exception was specimen with a
prolongedpregnancy (42 weeks) in which the percentage of NSE- (17%) andG-
(10%) cells was almost the same as at 6 weeks postnatally. Themaximal
quantitative difference of NEC was noted between 6- and8- week specimens
postnatally, e.g. 9% to 22% of G-cellsrespectively. Observations obtained
by NSE and S-100 protein werealso demonstrated in lymphoid cells of gut
associated andmesenteric lymphoid tissue.
Keywords: Human antrum, perinatal period, neuroendocrine cells, stereology, immunohistochemistry
- Type of paper
: Summary in proceedings
Title: Glycoconjugates and Regulatory Peptides in Bovine Abomasum
During Development
- Authors:
- Rode, Bojan (41266)
- Lacković-Venturin, Gordana (105166)
- Editors
- Stott, Elaine
Proceedings title: The Histochemical Journal
Language: engleski
Place: London, Velika Britanija
Year: 1992
ISBN/ISSN: 0018-2214
Pages: from 617 to 617
Meeting: 9th International Congress of Histochemistry and Cytochemistry
Held: from 08/30/92 to 09/05/92
Summary: Six lectins with different major sugar-binding affinities
wereinvestigated by indirect immunoperoxidase methods in paraffinsections
of bovine abomasum during fetal and postnatal period. Inaddition gastrin
and somatostatin were examined by PAP method inendocrine cells of same
specimens. Ulex europeaus I agglutinin(UEA I) which recognizes L-fucose
residue and Dolichos biflorusagglutinin (DBA) which recognizes
Nac-D-acetilgalactosamineproved to be the best markers of stomach glands
during theprocess of differentiation. In early stages of development (10
to14 wk of age) UEA I labels uniformely cell membranes ofepithelium
clusters and later on cytoplasmic compartments inseparate regions of
developing glands. With the progress ofdifferentiation at 20 to 22 wk of
age, DBA strongly labelsprimitive parietal cells at the bottom of crypts.
Postnatally andin adults DBA selectively labels intra- and
intercellularcanaliculi of parietal cells depending on the secretory
state.Stereological analysis of gastrin producing cells, which
firstappeared as singles cells at 16th wk of gestation, showed asignificant
increase of frequency (Vv=0.13 +- 0.001) and volumeof nuclei at 22th wk of
age. This coincided with the beginning ofparietal cell proliferation and
general growth of glands. Thefrequency of G-cells in adult mucosa reached
Vv=0.28+-0.001. Themultistep process of development of bovine stomach
mucosa isapparently accompanied by structural changes in glycoconjugatesand
presumably by trophic action of gastrin.
Keywords: Glycoconjugates, regulatory peptides, bovine abomasum, differentiation, immunoperoxidase method
- Type of paper
: Ph.D.
Title: Immunohistochemical and stereological study of the
neuroendocrine cells in the fetal antrum and duodenum of the rat and man
Faculty: Prirodoslovno-matematički fakultet Zagreb
Author: ADLER NEVENKA
Date of defense: 03/05/93
Language: hrvatski
Number of pages: 127
Summary: A correlative immunohistochemical and stereological study
ofneuroendocrine (NE) cells was carried out in antrum, duodenum andpancreas
of human fetuses with gestational ages from 18 to 42weeks and neonatuses 6
and 8 weeks old. Immunohistochemical studyof gut NE cells with
neurone-specific enolase (NSE) and S-100protein enables demonstration of
wide spectrum of NE cells andsimultanously autonomic nervous system, while
chromogranin Amakes possible detection of gut NE cells from the earliest
stagesof fetal life during ontogeny. The trophic action of antralgastrin
and somatostatin during fetal life is well known.Because these cells
represent the most numerous NE cells of theantrum, quantitative estimation
of human fetal gastrin G- andsomatostatin D-cells as well as G-cells in
rats (Y 59 strain) inearly postnatal period was carried out.
Comparativeimmunohistochemical and quantitative morphometric
studyrepresents the method of choice for research of topography,chronology
and frequency of gut NE cells and enables thereforethe interpretation of
differentiation and morphogenesis of thegut.
Keywords: Immunohistochemistry, stereology, neuroendocrine cells, human fetuses and neonatuses, rats neonatuses
