NEUROTRANSMITTERS IN THE REGULATION OF ADENOHYPOPHYSEOS
Main researcher
: KORŠIĆ, MIRKO (81592) Assistants
TEPAVČEVIĆ, DANILO (49335)
Type of research: basic Duration from: 01/01/91. to 12/31/95. Papers on project (total): 23
Papers on project quoted in Current Contents: 4
Institution name: Medicinski fakultet, Zagreb (108) Department/Institute: Division of endocrinology and metabolism Department of internal medicine Rebro School of medicine University of Zagreb Address: Kišpatićeva 12, 41 000 Zagreb, Croatia City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0) 01 27 12 80
Fax: 385 (0) 01 27 12 80
Phone: 385 (0) 01 2333 233 (274,220)
Summary: In the maintenance of adequate internal medium of
organism inspite of the constant, sometimes life threatening changes of
external enviroment, crucial is the regulation of cortisol secretion from
the adrenal glands, i.e., a normal functioning of
hypothalamus-pituitary-adrenal cortex axis. Despite the intensive
investigations of the role of CRH, ACTH, AVP and catecholamines over last
two decades, the understanding of interrelationship of above mentioned
regulatory mechanisms still remains incomplete and unclear, so we
consider indispensable to completly encompass the investigation of all
factors in the regulation of hypothalamus-pituitary-adrenal cortex axis.
Elaboration of a new and original test procedure for examination of
capacity and reserve of ACTH secretion by means of combining main
hypothalamic factors (CRH, AVP) and taking into consideration the
influence of "daily rhythm" on hormonal secretion, we regard important to
get a more certain differential diagnosis of states with impaired
hypothalamic-pituitary axis, along with the clearer conception, i.e.,
delineation between the interference of CRH and AVP. New insights into
the secretory activity and biological behaviour of crucial cells in the
neuroendocrine reactions - corticotroph cells, we hope to get by
investigating the ACTH secreting cells culture from active hypophyseal
adenomas. Effects of various hypothalamic factors, neurotransmitters and
other "test factors" (e.i. a new organoprotector-BPC) under various
experimental conditions in cell cultures, we hope to elucidate the
mechanism of cellular communications.
Research goals: Multicellular organisms are employing different
mechanisms of intercellular communications to adjust the influences of the
external and internal enviroments with the aim of the survival. The
function of neuroendocrine system is the basis of that mechanism, and the
main factor in homeostatsis of living organisms is
hypothalamus-pituitary-adrenal axis, because the cortisol is indispensable
for survival. Due to complexity of the system as well as partial and
one-sided ways of approach to this problem, the connection of many factors
of hypothalamic, neurohypophysial and peripheral origin in the regulation
of anterior pituitary hormone secretion, especially of
adrenocorticotropin (ACTH) remains unclear. The general aim of our
investigation is to comprise all factors in these processes - the
releasing factors of hypothalamus, hormones of anterior pituitary lobe and
the peripheral hormones of the endocrine glands, especially the adrenal
cortex. By means of a detailed elaboration of the normal subjects as
well as of patients with hypercortisolism of different etiology, we intend
to create a new test for the investigation of the capacity and reserve of
the anterior pituitary gland concerning the ACTH secretion, which might be
helpfull for the diagnosis of hypercortisolism in humans; by simultaneous
application of afixed dose of 100 ug CRH iv together with different doses
of AVP (5 and 10 IU im), by performing the tests in the morning and also
in the afternoon, we hope to avoid the influence of different
refractoriness of hypothalamic/pituitary receptors. The results should be
corrected with the control ITT, CRH and AVPtest. On behalf of the more
complete investigation of biological activity and biologic behaviour of
ACTH cells of secreting pituitary adenomas we plan to investigate their
responses to various hypothalamic factors and neurotransmitters (GABOB,
RIT, IGF), and some new organoprotectors (BPC) under different
experimental conditions incell cultures. Both aspects of our study have
also a basically scientific character.
COOPERATION - PROJECTS
Name of project
: 3-01-030 Morfološke i funkcionalne osobine
adenoma hipofize Name of institution: Medicinski fakultet, zagreb City: 10000 - Zagreb, Croatia
COOPERATION - INSTITUTIONS
Name of institution
: INSTITUT "RUĐER BOŠKOVIĆ" Type of institution: State institute Type of cooperation: Joint publishing of scientific papers City: 10000 - Zagreb, Croatia Other information about the project.