SVIBOR - Project code: 3-01-082


Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69


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Project code: 3-01-082


Main researcher: KORŠIĆ, MIRKO (81592)

Type of research: basic
Duration from: 01/01/91. to 12/31/95.

Papers on project (total): 23
Papers on project quoted in Current Contents: 4
Institution name: Medicinski fakultet, Zagreb (108)
Department/Institute: Division of endocrinology and metabolism Department of internal medicine Rebro School of medicine University of Zagreb
Address: Kišpatićeva 12, 41 000 Zagreb, Croatia
City: 10000 - Zagreb, Croatia
Phone: 385 (0) 01 27 12 80
Fax: 385 (0) 01 27 12 80
Phone: 385 (0) 01 2333 233 (274,220)

Summary: In the maintenance of adequate internal medium of organism inspite of the constant, sometimes life threatening changes of external enviroment, crucial is the regulation of cortisol secretion from the adrenal glands, i.e., a normal functioning of hypothalamus-pituitary-adrenal cortex axis. Despite the intensive investigations of the role of CRH, ACTH, AVP and catecholamines over last two decades, the understanding of interrelationship of above mentioned regulatory mechanisms still remains incomplete and unclear, so we consider indispensable to completly encompass the investigation of all factors in the regulation of hypothalamus-pituitary-adrenal cortex axis. Elaboration of a new and original test procedure for examination of capacity and reserve of ACTH secretion by means of combining main hypothalamic factors (CRH, AVP) and taking into consideration the influence of "daily rhythm" on hormonal secretion, we regard important to get a more certain differential diagnosis of states with impaired hypothalamic-pituitary axis, along with the clearer conception, i.e., delineation between the interference of CRH and AVP. New insights into the secretory activity and biological behaviour of crucial cells in the neuroendocrine reactions - corticotroph cells, we hope to get by investigating the ACTH secreting cells culture from active hypophyseal adenomas. Effects of various hypothalamic factors, neurotransmitters and other "test factors" (e.i. a new organoprotector-BPC) under various experimental conditions in cell cultures, we hope to elucidate the mechanism of cellular communications.

Keywords: neurotransmitters, pituitary hormones, hypothalamic - anterior pituitary axis

Research goals: Multicellular organisms are employing different mechanisms of intercellular communications to adjust the influences of the external and internal enviroments with the aim of the survival. The function of neuroendocrine system is the basis of that mechanism, and the main factor in homeostatsis of living organisms is hypothalamus-pituitary-adrenal axis, because the cortisol is indispensable for survival. Due to complexity of the system as well as partial and one-sided ways of approach to this problem, the connection of many factors of hypothalamic, neurohypophysial and peripheral origin in the regulation of anterior pituitary hormone secretion, especially of adrenocorticotropin (ACTH) remains unclear. The general aim of our investigation is to comprise all factors in these processes - the releasing factors of hypothalamus, hormones of anterior pituitary lobe and the peripheral hormones of the endocrine glands, especially the adrenal cortex. By means of a detailed elaboration of the normal subjects as well as of patients with hypercortisolism of different etiology, we intend to create a new test for the investigation of the capacity and reserve of the anterior pituitary gland concerning the ACTH secretion, which might be helpfull for the diagnosis of hypercortisolism in humans; by simultaneous application of afixed dose of 100 ug CRH iv together with different doses of AVP (5 and 10 IU im), by performing the tests in the morning and also in the afternoon, we hope to avoid the influence of different refractoriness of hypothalamic/pituitary receptors. The results should be corrected with the control ITT, CRH and AVPtest. On behalf of the more complete investigation of biological activity and biologic behaviour of ACTH cells of secreting pituitary adenomas we plan to investigate their responses to various hypothalamic factors and neurotransmitters (GABOB, RIT, IGF), and some new organoprotectors (BPC) under different experimental conditions incell cultures. Both aspects of our study have also a basically scientific character.


  1. Name of project: 3-01-030 Morfološke i funkcionalne osobine adenoma hipofize
    Name of institution: Medicinski fakultet, zagreb
    City: 10000 - Zagreb, Croatia


  1. Name of institution: INSTITUT "RUĐER BOŠKOVIĆ"
    Type of institution: State institute
    Type of cooperation: Joint publishing of scientific papers
    City: 10000 - Zagreb, Croatia

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Last update: 10/05/95