SVIBOR - Project code: 3-01-083

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 3-01-083


Tumor cell cycle in malignant tumors of female genitals


Main researcher: JUKIĆ, STANKO (99482)


Assistants
Type of research: applied
Duration from: 04/30/91. to 12/15/95.
Papers on project (total): 63
Papers on project quoted in Current Contents: 12
Institution name: Medicinski fakultet, Zagreb (108)
Department/Institute: Medical university Gynaecological and obstertrics clinic Department for gynaecology and perinatal pathology
Address: Petrova 13
City: 10000 - Zagreb, Croatia
Communication
Fax: 385 (0)1 444-279
Summary: The cell cycle of tumor cells will be studied by means of flow cytometry. The abnormalities in DNA contents, the dynamics of 17-beta estradiol and izocytric dehydrogenase and the levels of steroid hormone receptors will be assesed as the prognostic factors in certain malignant gynecological tumors. These tests would contribute to better understanding of the biological behaviour, character and agressivity of these diseases. They would also contribute to planning the extent, means and duration of therapy, ways of follow up and improving the five year survival of the patients. The study will encompass premalignant and malignant lesions of the uterine cervix, endometrium, ovaries, uterine sarcomas and trophoblastic disease. Fifty to 100 patients will be enclosed in every investigated group.

Keywords: malignant genital tumors, flow cytometry, DNA-index, dehydrogenase enzymes, steroid receptors, prognostic factors

Research goals: The aim of this study is to learn more about the behaviour and characteristics of malignant gynecological tumors using three different laboratory methods seldom used abroad and never before in our country. There were never used before concurrently and together in the same patients. The additional differentiation of the grade of malignancy of the tumors is important for several reasons: 1. to get to know better the biology of these tumors and 2. because the criteria of malignancy used to determine the prognosis and therapeutic strategy are not perfect, influencing thus the outcome of the disease. The diagnostic methods that will be used are: 1. Flow cytometry - to study the tumor cell cycle, because the malignant cell proliferation is mostly accompanied by the changes in genetic expression and DNA contents. The analysis of the histogram enables the insight into the grade of proliferation, mitoses and tumor cells ploidy. 2. Monoclonal antibodies - in the premalignant and malignant changes of the endometrium, the dynamics of steroid hormone receptors, 17 beta estradiol and izocytric dehydrogenase will be evaluated, 3. Comparison of the prognostic importance of the determination of tumor cell cycle (by flow cytometry), steroid hormone receptor levels and 17-beta estradiol and izocytric dehydrogenase levels with other clinical prognostic factors in different tumors, with the aim to estimate the therapeutic approach, natural history and the outcome of the malignant disease.

COOPERATION - INSTITUTIONS


  1. Name of institution: St. Thomas Hospital Medical School in London
    Type of institution: Institution whose primary activities ar
    Type of cooperation: Joint publishing of scientific papers
    City: London, Engleska

  2. Name of institution: Institut "Ruđer Bošković"
    Type of institution: State institute
    Type of cooperation: Joint publishing of scientific papers
    City: 10000 - Zagreb, Croatia

  3. Name of institution: Klinički bolnički centar
    Type of institution: Institution whose primary activities ar
    Type of cooperation: Joint publishing of scientific papers
    City: 10000 - Zagreb, Croatia

  4. Name of institution: The University of Kansas Medical Center
    Type of institution: University/Faculty
    Type of cooperation: Occasional exchange of information
    City: Kansas City, Kansas, USA

  5. Name of institution: Universitaet Klinic Wien
    Type of institution: University/Faculty
    Type of cooperation: Joint publishing of scientific papers
    City: Wien, Austria
Other information about the project.
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