SVIBOR - Papers quoted in CC - project code: 3-01-089
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Papers quoted in Current Contents on project 3-01-089
Quoted papers: 5
Other papers: 6
Total: 11
Title: Number of follicle, oocytes and embryos in human in vitro
fertilization is relative to serum estradiol and progesterone patterns
during different types of ovarian hyperstimulation
- Authors:
- Suchanek, Ernest (98740)
- Huderer, Koraljka
- Dobec, Danko
- Hlavati, Višnja
- Šimunić, Velimir (76901)
- Grizelj, Veselko (14595)
Journal: European Journal of Obstetrics & Gynecology and
Reproductive Biology
ISSN: 0028-2243
Volume: 56
Year: 1994
Pages: from 121 to 127
Number of references: 28
Language: engleski
Summary: Preovulatory serum estradiol and progesterone levels as
well as their ratio were compared in different types of ovulation induction
in order to determine whether these findings could be used to predict the
number of preovulatory follicles, number of oocytes aspirated and embryos
obtained. Significantly more oocytes were retrived by follicular aspiration
and significantly more embryos developed in patients receiving
gonadotropin-releasing hormone agonist and human menopausal gonadotropins
than in those given other ovulation inductors. on days -2 and -1 of the
cycle, serum estradiol levels were significantly lower in pure
follicle-stimulating hormone induction. Serum progesterone was
significantly higher in pure follicle-stimulating hormone cycle on days -4
and -3. In clomiphene citrate and human menopausal gonadotropin induction,
progesterone levels were significantly lower on days -2 and -1, and on the
day of follicular aspiration. Ratios of estradiol/progesterone were lower
in pure follicle-stimulating hormone group from day -3 to day -1 of the
cycle. A significant correlation was found between estradiol and
progesterone serum levels and the numbers of preovulatory follicles,
oocytes and embryos. The study revealed the usefulness of serum estradiol
and progesterone determinations in assisted reproduction.
Keywords: Menstrual cycle, Clomiphene citrate, Human menopausal gonadotropins, Gonadotropin-releasing hormone agonist, pFSH
Title: Follicular fluid contents of hyaluronic acid,
follicle-stimulating hormone and steroids relative to the success of in
vitro fertilization of human oocytes
- Authors:
- Suchanek, Ernest (98740)
- Šimunić, Velimir (76901)
- Juretić, Dubravka (19264)
- Grizelj, Veselko (14595)
Journal: Fertility and Sterility
Number: 2
ISSN: 0015-0282
Volume: 62
Year: 1994
Pages: from 347 to 352
Number of references: 25
Language: engleski
Summary: The objective was to determine the concentrations of
hyaluronic acid, FSH, P, and E2 in the follicular fluid (FF) obtained from
IVF-ET patients and to assess the value of these measurments in predicting
the outcome of fertilization. One hundred eleven samples were
retrospectively analyzed for the hyaluronic acid and hormone contents.
Preovulatory FF samples were collected from 67 women undergoing IVF-ET
treatment because of tubal absence or obstruction. The FF hyaluronic acid
and hormone concentrations were compared according to the type of ovulation
induction, a significantly lower hyaluronic acid concentrations was found
in FF harvested from the patients treated with GnRH agonist-hMG. No
significant correlation was found between FF hyaluronic acid and either
morphological maturity of the oocyte-cumulus complex or fertilizability of
oocytes. The level of FSH was significantly higher in FF, yielding a mature
oocyte-cumulus complex and from which the oocyte obtained successfully
fertilized and cleaved. A significant increase in the E2 concentrations was
found in FF in which mature cumuli oophori were present. The levels of
hyaluronic acid significantly correlated with FSH in FF. Expansion of the
human oocyte-cumulus cell complex is an FSH-dependent phenomenon. The data
are in agreement with the hypothesis that intrafollicular FSH plays an
important role in the secretion of hyaluronic acid by granulosa cells and
may act synergistically with E2 to enhance cytoplasmatic maturation,
resulting in successful fertilization.
Keywords: Follicular fluid, hyaluronic acid, FSH, estradiol, progesterone, ovarian stimulation, in vitro fertilization
Title: Milk versus test-yolk-preincubated sperm; in vitro
fertilization outcome
- Authors:
- Barišić, Dubravko (300207)
- Suchanek, Ernest (98740)
- Jeyendran, Rajasingam S.
Journal: European Journal of Obstetrics & Gynecology and
Reproductive Biology
Number: 1
ISSN: 0028-2243
Volume: 63
Year: 1995
Pages: from 66 to 68
Number of references: 19
Language: engleski
Summary: Preincubation of sperm in TEST-yolk medium enhances in
vitro fertilization (IVF) outcome. However, preincubation of sperm in milk
at 5 C enhances the results of sperm penetration assay and hemizona assay.
This study was therefore performed to determine the influence of milk on in
vitro fertilization rate of human oocytes, as compared to TEST-yolk medium.
During study, 41 consecutive couples undergoing an IVF procedure were
randomized. Of these, 20 couples were admitted for the milk study (group
1), and 21 couples for the TEST-yolk study (group 2). Each ejaculate was
Percoll-processed and the sperm pellet was resuspended in 0.5 ml of culture
medium. An equal volume of heat-inactivated homogenized cow's milk (95 C,
10 min) was added to sperm suspension from group 1, and an equal volume of
TEST-yolk medium was added to sperm suspension from group 2. After 2 hours
of incubation at 5 C, and washing with culture medium at 37 C, oocytes were
inseminated. Oocytes from group 1 couples were inseminated with
milk-treated spermatozoa, and those from group 2 couples with yolk-treated
spermatozoa. Oocytes were evaluated for fertilization after 18 hours. Sperm
preincubated in milk fertilized 75 out of 100 mature oocytes (75%).
TEST-yolk-treated sperm fertilized 45 out of 64 mature oocytes (70%). The
difference was not statistically significant. Preincubation of spermatozoa
in milk, as compared with preincubation in TEST-yolk medium yields a
similar IVF outcome, so milk may be a suitable alternative medium for
preincubation of spermatozoa to enhance its fertilizing potential.
Keywords: In vitro fertilization, Sperm preincubation, Milk, TEST-yolk
Title: The effect of preincubation of sperm in TEST-yolk medium
on in vitro fertilization outcome
- Authors:
- Barišić, Dubravko (300207)
- Dobec, Danko
- Hlavati, Visnja
- Suchanek, Ernest (98740)
Journal: Gynaecologia et perinatologia
Number: 4
ISSN: 1330-0091
Volume: 3
Year: 1994
Pages: from 139 to 141
Number of references: 21
Language: hrvatski
Summary: Preincubation of human spermatozoa in TEST-yolk medium
enchances in vitro fertilization (IVF) outcome, when compared to standard
semen processing (swin-up). To evaluate the efficiency of TEST-yolk medium,
fertilization rates, obtained after sperm incubation in TEST-yolk were
compared to those obtained after swim-up. Ejaculates of 21 couples, that
consequently entered the IVF program in one month regardless the
indication, were preincubated in TEST-yolk at 5 C before the insemination
of oocytes. 73 couples, consequently entered in the IVF program, in the
month before, regardless the indication, were the control (swim-up) group.
Ejaculates in the control group were washed (400g x 10 min) in Ham F-10
medium, supplemented with 10% fetal cord serum or Menezo B2 medium. After
60 minutes, the upper layer of medium, containing motile sperm, was
removed. Sperm samples, containing 1,5x10 5/mL motile sperm were prepared,
and oocytes inseminated with them. After 15-18 hours of co-incubation of
gametes, oocytes were evaluated for fertilization, defined as finding of 2
pronuclei. Ejaculates in the study (TEST-yolk medium group) were identicaly
processed, except for preincubation in TEST-yolk medium. Shortly, after
swim-up an equal volume of TEST-yolk medium was added to the sperm sample,
the suspension was cooled slowly to 5 C and incubated for 2 hours, followed
by washing in culture medium at 37 C (400g x 10 min), twice. There was no
significant difference in total sperm concentration, progressive sperm
concentration, and in percentage of male factor patients between the
groups. Sperm preincubated in TEST-yolk medium fertilized significanly
(p<0,05) higher percent of oocytes (70%, 45 out of 64), as compared to
sperm processed only by swim-up (59%, 175 out of 296). Fertilization rates
in subgroups of patients with male factor, according to the WHO standards,
were significantly (p<0,05) different (TEST-yolk medium group: 40%, swim-up
group: 31%).
Keywords: Sperm processing, TEST-yolk medium, In vitro fertilization
Title: Culture medium type and supplements modulate estradiol and
progesterone production by granulosa cells in vitro
- Authors:
- Huderer, Koraljka
- Dobec, Danko
- Suchanek, Ernest (98740)
Journal: Journal of Assisted Reproduction and Genetics
Number: 3
ISSN: 1058-0468
Volume: 12
Year: 1995
Pages: from 8S to 8S
Language: engleski
Summary: The objective was to investigate the influence of culture
medium type and serum, as well as serum replacement supplement on E2 and P4
release by granulosa cells (GCs) isolated from 25 patients undergoing IVF
treatment and receiving hMG (Pergonal) and hCG (Profasi) for ovulation
induction. In 4 days long culture with every 24 hours medium replacement,
we tested 4 media: minimum essential medium (MEM), nutrient mixture F-10
(HAM), medium 199 (M199) and Inra Menezo B2 (MZO), all with 7 and 15%
newborn cord serum (CS). We also tested Controlled process serum
replacement (CPSR-1, Sigma, USA) as a 10% supplement to MZO medium.
Estradiol production was significantly lower in MEM+7%CS, M199+7%CS and
MZO+7%CS cultures compared with MEM+15%CS on day 3 (p<0.05). On the 3th and
4th day GCs cultured in MZO+7%CS released significantly lower E2 levels
than in HAM+15%CS, M199+15%CS and MEM+15%CS (p<0.05).P4 release was
significantly higher in MZO+15%CS on day 1 compared with HAM+7%CS and on
day 3, compared with MEM+7%CS (p<0.05). In serum free culture, E2 and P4
production was significantly lower than in all others (p<0.05). This study
shows that culture media has a significant influence on E2 and P4
production and, by that, on in vitro GCs luteinization and oocyte
maturation which is of great interest in assisted reproduction. The
difference in GCs steroidogenesis modulation between serum and serum-free
cultures indicate that we may prefer media with serum replacement in IVF
procedure.
Keywords: granulosa cells, progesterone, estradiol, in-vitro fertilization
Information: svibor@znanost.hr
