BIOCHEMISTRY AND PHARMACOLOGY OF PERIOVULATORY OVARIAN FOLLICLE IN WOMAN
Main researcher
: SUCHANEK, ERNEST (98740) Assistants
GRIZELJ, VESELKO (14595)
ŠIMUNIĆ, VELIMIR (76901)
BARIŠIĆ, DUBRAVKO (300207)
TANDARA, MARIJAN (900013)
HUDERER, KORALJKA (900498)
Type of research: applied Duration from: 04/30/91. to 12/31/95. Papers on project (total): 11
Papers on project quoted in Current Contents: 5
Institution name: Medicinski fakultet, Zagreb (108) Department/Institute: Department of Clinical Biochemistry and Reproductive Biology University Clinic of Obstetrics and Gynecology Address: Petrova 13 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 44 40 22
Fax: 385 (0)1 44 42 79
Phone: 385 (0)
Summary: To determine the concentrations of hyaluronic acid,FSH,
progesterone and estradiol in the follicular fluid obtained from in vitro
fertilization-embryo transfer (IVF-ET) patients and to assess the value
of these measurements in predicting the outcome of fertilization. One
hundred and eleven samples were retrospectively analyzed for the
hyaluronic acid and hormone contents. Preovulatory follicular fluid
samples were collected from 67 women undergoing IVF-ET treatment due to
tubal absence or obstruction. The follicular fluid hyaluronic acid and
hormone concentrations were compared according to the type of ovulation
induction, follicular development, and IVF outcome. According to the type
of ovulation induction, a significantly lower hyaluronic acid concentration
was found in follicular fluid harvested from the patients treated with
GnRH-agonist-human menopausal gonadotropins. No significant correlation was
found between follicular fluid hyaluronic acid and either morphologic
maturity of the oocyte-cumulus complex or fertilizability of oocytes. The
level of FSH was significantly higher in follicular fluids yielding a
mature oocyte-cumulus complex, and from which the oocyte obtained
successfully fertilized and cleaved. A significant increase in the
estradiol concentration was found in follicular fluid in which mature
cumuli oophori were present. The levels of hyaluronic acid significantly
correlated with FSH in follicular fluid. Expansion of the human
oocyte-cumulus cell complex is a FSH-dependent phenomenon. The data are in
agreement with the hypothesis that intrafollicular FSH plays an important
role in the secretion of hyaluronic acid by granulosa cells and may act
synergistically with estradiol to enhance cytoplasmic maturation resulting
in successful fertilization.Preovulatory serum estradiol and progesterone
levels as well as their ratio were compared in different types of ovulation
induction in order to determine whether these findings could be used to
predict the number of preovulatory follicles, number of oocytes aspirated
and embryos transferred. Significantly more oocytes were obtained by
follicular aspiration and significantly more embryos transferred to the
uterus in patients receiving GnRH-agonist and human menopausal
gonadotropins than in those given other ovulation inductors. On days -2 and
-1 of the cycle, serum estradiol levels were significantly lower in pure
FSH induction. Serum progesterone was significantly higher in pure FSH
cycles on days -4 and -3. In clomiphene citrate and human menopausal
gonadotropin induction, progesterone levels were significantly lower on
days -2 and -1, and on the day of follicular aspiration. Ratios of
estradiol/ progesterone were lower in pure FSH group from day -3 to day -1
of the cycle. A significant correlation was found between estradiol and
progesterone serum levels and the numbers of preovulatory follicles,
oocytes and embryos as well. The study revealed the usefulness of serum
estradiol and progesterone determinations in assisted reproduction.
Investigation was performed on estradiol and progesterone syntheses from
granulosa cell culture concerning the composition of media used in oocyte
and embryo culture. Menezo B2 and MEM media with the supplementation of 7%
and 15% of newborn cord serum were used. Ovulation induction with human
menopausal gonadotropins was performed in 11 women. From the 50 000
granulosa cells isolated the production of estradiol and progesterone
during 4 days of culture was determined. The estradiol concentration was
significantly higher in both media when 15% cord serum was used.
Comparing the media with the same amount of cord serum no difference in
estradiol production was noted. Higher progesterone synthesis was found
in Menezo B2 with 7% than 15% cord serum supplementation. Progesterone was
lower from the culture of granulosa cells in MEM than Menezo B2 with the
same amount of cord serum. The results revealed the data that the
composition of medium used for human oocyte and embryo culture is very
important in the view of the success of assisted reproduction.
Research goals: The aim of this study is to determine biochemical
markers in serum, preovulatory follicular fluid and granulosa cells culture
as well, to obtain data about oocyte maturity and their ability to
fertilize in vitro. The results will be used for the type of
pharmacological ovulation induction management and for endocrine
environment composition which is of great importance for ovarian follicle
physiology.
COOPERATION - INSTITUTIONS
Name of institution
: Department of Obstetrics and Gynecology,
Northwestern University Medical School and Andrology Laboratory Services,
Inc. Type of institution: University/Faculty Type of cooperation: Joint publishing of scientific papers City: 60611 - Chicago, Illinois, S.A.D. Other information about the project.