SVIBOR - Project code: 3-01-117

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 3-01-117


Gas chromatography in microbiology


Main researcher: FRANOTOVIĆ, MIRA (126140)


Assistants
Type of research: applied
Duration from: 04/30/91. to 12/31/95.
Papers on project (total): 4
Papers on project quoted in Current Contents: 2
Institution name: Zavod za zaštitu zdravlja Republike Hrvatske, Zagreb (5)
Department/Institute: Microbiology service
Address: Rockefellerova 7
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0) 1 272-822 /94
Fax: 385 (0) 1 272-166
Phone: 385 (0) 1 272-822/ 85
Fax: 385 (0) 1 277-481
Fax: 385 (0) 1 277-481
Phone: 385 (0) 1 272-822
Phone: 385 (0) 1 272-282 / 85
Summary: Gas chromatography (GC) is one of the more recent analytical methods. For its rapidity and sensitivity it is used to analyse and identify various organic compounds. Developed abroad in the 60s as a method of microorganism identification as well, it became a precious tool in microbiology. In our laboratory GC analysis of cellular fatty acids in Mycobacterium tuberculosis as well as some aerobic and anaerobic bacteria has been introduced.Characteristic profiles of fatty acids of examined microorganisms have been obtained.The presence of tuberculostearic acid (TBSA) as a specific mycobacterial lipid component, which enables more rapid identification of the causative agent of tuberculosis, was also found and confirmed.

Keywords: Gas chromatography (GC), microbiology, identification, cellular fatty acids, M.tuberculosis, tuberculostearic acid (TBSA)

Research goals: Basically, the project aims at widening the knowledge of some medically important microorganisms (mycobacteria, aerobic and anaerobic bacteria) from the biochemical standpoint, familiarizing the staff with the recent microorganism identification methods and at applying them routinely. To introduce gas chromatography in microbiology is to expand its range of identification methods and hence increase the reliability of microbiological findings. Other anticipated possible benefits are quicker detection and identification of the causative agents of infectious diseases in clinical materials.
Other information about the project.
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Last update: 10/18/95
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