- Type of paper
: Book
Title: Dry chemistry methods in diagnostics
- Authors:
- Topić, Elizabeta (50266)
- Zadro, Renata (95160)
- Editors
- Topić, Elizabeta (50266)
- Zadro, Renata (95160)
Publisher: Meditrade-Zagreb
ISBN: 953-96445-0-X
Year: 1995
Number of pages: 165
Number of references: 15
Language: hrvatski
Summary: Dry chemistry has been accepted as an important technology
in medical laboratories forr many years. Many evaluations of this
technology have been undertaken by reputable laboratories, the results of
which were excellent when compared with conventional wet chemistry
analysis. This book contains a detailed overview of the current knowledge
in the field of dry chemistry and a description of various commercially
available dry chemistry systems as Ektachem, Reflotron, Seralyzer, Cobas
Ready, Drichem, Opus and Stratus. Since this newly introduced and used dry
chemistry methods has a tendency to be a diagnostic tool not only in
medical laboratory but also in physician offices, the book underlines the
importance of knowing the the bases of analitical, biochemical and
pathobiochemical proceses.
Keywords: Dry Chemistry, Ektachem, Reflotron, Seralyzer, Opus, Cobas Ready, Stratus, Drichem
Other: Analiti;ki sistemi po na;elu suhe kemije rabe se u naŠoj
domovini ve' 30.-tak godina. Ova knjiga prvi puta u naŠoj zemlji detaljno
opisuje sve dosada razvijene sustave suhe kemije; njihova na;ela,
tehnologiju i analiti;ke instrumente, sustave suhih reagencija te ukazuje
na preanaliti;ke, analiti;ke i poslije analiti;ke greŠke koje mogu nastati
nedovoljnim poznavanjem metodoloŠkih zahtjeva.
- Type of paper
: Paper in journal
Title: Hepatopoietin-like activity in patients with liver disease
- Authors:
- Topić, Elizabeta (50266)
- Zadro, Renata (95160)
- Gluhak, Jelica (160474)
- Slijepčević, Milivoj (43700)
- Ruhenstroth-Bauer, Gerhard
- Vogl, Siegfrid
- Hoffmann, Gerhard
Journal: Exp Clin Gastroenterol
Number: 3
ISSN: 0353-9245
Volume: 1
Year: 1991
Pages: from 221 to 227
Number of references: 18
Language: engleski
Summary: A crude rat liver extract called hepatopoietin, isolated
from regenerating rat livers and from plasma 14 h after partial
hepatectomy, induced a two-fold increase in DNA synthesis in hepatocytes in
primary culture but an increase only in the S phase of cell cycle. The
blood plasma of patients with liver diseases contained a liver
growth-promoting factor similar to hepatopoietin. Human plasma
hepatopoietin showed the same properties (acid- and heat-stable, Mm 38+5
kD) but was some what more effective than hepatopoietin isolated from rat
liver, i.e.2.5-3.5-fold, in inducing proliferation.
Keywords: Growth promoting factor, hepatocyte proliferation, hepatocarcinoma, hepatopoietin, hepatocyte primary culture
- Type of paper
: Paper in journal
Title: Method for purification of human genomic DNA from whole
blood
- Authors:
- Topić, Elizabeta (50266)
- Gluhak, Jelica (160474)
Journal: Clinica e Laboratorio
Number: 4
ISSN: 0391-2035
Volume: 15
Year: 1991
Pages: from 135 to 138
Number of references: 13
Language: engleski
Summary: The aim of this study was to isolate a restrictible DNA,
i.e. an adequately pure and intact DNA for Southern blot analysis or
analysis of restriction fragments from whole blood samples.
The quality of isolated DNA suitable for digestion with restriction enzymes
was estimated by digestion of isolated DNA with restrictive endonucleases
HIND III, Bam HI and Eco RI, and the restricted fragments were separated
electrophoretically in 1% agarose gel. The results suggested that the DNA
obtained by the procedure described appears to be essentially pure and
suitable for numerous applications, when even stored at -20 C for 6 months.
Keywords: Isolation of DNA, minigel electrophoresis, restriction endonucleases, stability of DNA
- Type of paper
: Paper in journal
Title: Ser Asp Lys-Ac, a strong inhibitor of liver cell
proliferation
- Authors:
- Ruhenstroth-Bauer, Gerhard
- Vogl, Siegfrid
- Voelter, Wolfgang
- Topić, Elizabeta (50266)
Journal: Naturwissenschaften
Volume: 80
Year: 1993
Pages: from 314 to 315
Number of references: 15
Language: engleski
Summary: In the course of the investigation of hepatopoietin, we
discovered that, in vivo and in vitro, the tripeptide Ser Asp Lys-Ac
(SDK-Ac) in minimal amounts inhibits the proliferation of hepatocytes very
intensively. The results on Wistar rats (100 +5g), subjected to 2/3 partial
hepatectomy, and rats with Yoshida liver tumor, both given an i.p.
injection of 1 ug SDK-Ac dissolved in 1 ml saline, showed that the
tripeptide exhibited a 47%-57% decrease in the proliferation activity of
the remnant liver cells 10 h after partial hepatectomy. The mean amount of
ascites and the mean number of Yoshida cells in the ascites were less than
20%.
Keywords: Tripeptide Ser Asp Lys-Ac, SDK-Ac, Inhibition of hepatocytes proliferation, Yoshida liver tumor
- Type of paper
: Paper in journal
Title: Liver specific growth factor - hepatopoietin
- Authors:
- Topić, Elizabeta (50266)
- Zadro, Renata (95160)
- Paripović, Roberta
- Gluhak, Jelica (160474)
- Barilar-Antoljak, Nataša (178140)
- Bjelinski, Dijana (177060)
- Popović, Jasna
- Slijepčević, Milivoj (43700)
- Ruhenstroth-Bauer, Gerhard
- Vogl, Siegfrid
Journal: Il Friuli Medico, Alpe Adria Journal of Medicine
Number: 2
ISSN: 0016-1535
Volume: 48
Year: 1993
Pages: from 215 to 229
Number of references: 20
Language: engleski
Summary: A rat liver containing an active substance, hepatopoietin,
isolated from liver remnant 50 h after partial hepatectomy, displays a
capacity to stimulate DNA synthesis in hepatocyte primary culture. Further
purification and identification of the crude hepatopoietin extract were
achieved using Sephadex G-100 chromatography, SDS-PAGE, IEF-PAGE and
two-dimensional electrophoresis. Specific antibodies to the crude rat liver
hepatopoietin extract were produced by rabbit and tested by Western blot.
Keywords: Hepatopoietin, liver growth factor, SDS-PAGE, IEF-PAGE, 2D-electrophoresis, Western blot
- Type of paper
: Paper in journal
Title: SDS-PAGE separation of hepatopoietin protein bands
- Authors:
- Topić, Elizabeta (50266)
- Petra;, Dubravko
- Gluhak, Jelica (160474)
- Zadro, Renata (95160)
- Slijepčević, Milivoj (43700)
- Ruhenstroth-Bauer, Gerhard
- Paripović, Roberta (178746)
Journal: Clinica e Laboratorio
Number: 2
ISSN: 0391-2035
Volume: 19
Year: 1995
Pages: from 41 to 46
Number of references: 17
Language: engleski
Summary: Human plasma hepatopoietin (hPHP) extract was isolated from
72 liver disease patients and 25 healthy volunteers. Protein bands of hPHP
extract were electrophoretically separated bz SDS-PAGE modified so as to
allow good separation of proteins between Mr 30.000 and 50.000. The
electrophoretic pattern showed three protein bands in this range, i.e.
bands A,B and C, with Mr of about 41.700, 38.000, 33.800, respectively.
Positive band A was found in 92 %, band B in 97%, and band C in 85% of
patients. In the control group, 80% of subjects had positive band A, 88%
band B, and 80% band C. When the bands were compared to the results of
biochemical parameters commonly used to test liver function, they showed
the highest frequency of band A in patients with ALT and LDH values bz 3SD
above the mean, whereas the highest frequency of band B was found in
patients with TB, AP, AST, GGT, AFP and CHE values by 3 SD exceeding the
mean value of the reference interval. Band C was observed to have lowest
frequency, regardless of the biochemical parameter evaluated.
Keywords: hepatopoietin, hepatocyte growth factor, SDS-PAGE, liver disease
- Type of paper
: Paper in journal
Title: Evaluacija Kodak Ektachem 500 sustava suhe kemije
- Authors:
- Topić, Elizabeta (50266)
- Alpeza, Ines
- Barbari', Vesna
- :unovi', Miljenka
Journal: Klinisches Labor
Number: 11
ISSN: 0941-2131
Volume: 39
Year: 1993
Pages: from 895 to 904
Number of references: 13
Language: engleski
Summary: The Ektachem 500 clinical chemistry analyzer is a discrete
random access analyzer performing most common clinical chemistry tests
including electrolyte measurement by direct potentiometry. The system
evaluated accordimg to the ECCLS protocol, showed that the imprecision,
accuracy and linearity of the methods are satisfactory. The results
measered by above system corelate very well with those obtained by
conventional wet chemistry methods. Besides, the component of dry reagent
slide to eliminate the endogenous (hemoglobin, protein and bilirubin) as
well egzogenous (therapeutic drugs) interferences, allows the tests to be
performed with high analytical precision, accuracy, sensitivity and
specificity which is of utmost importace for every day routine and research
work.
Keywords: Ektachem 500, dry chemistry system, method evaluation
- Type of paper
: Paper in proceedings
Title: Isolation and identification of nucleic acids
- Authors:
- Topić, Elizabeta (50266)
- Editors
- Salamuni', Ilza
- Dujmov, Ivana
Proceedings title: Molekularna biologija i primjena molekularno bioloŠkih metoda u klini;ko laborat
Language: hrvatski
Place: Split
Year: 1994
Pages: from 20 to 28
Meeting: 1. Stru;ni skup medicinskih biokemi;ara Hrvatske
Held: from 09/17/94 to 09/20/94
Summary: Over the past 20 years, recombinant DNA technology has
developed into a potent and sensitive technique for the study of human
diseases, not only immensely improving the basic knowledge about molecular
disturbance in human diseases, but also ever more influencing the diagnosis
of the diseases. A diagnostic procedure using the recombinant DNA
technology includes the following steps: isolation of target DNA or RNA and
preparation of their probes, molecular hybridization, hybrid detection and
interpretation of the results. The methods of DNA and RNA isolation,
quality testing of the isolated nucleic acid, molecular hybridization,
hybrid detection, and eventually, results interpretation are descriebed.
The most frequently used methods of DNA technology and their diagnostic
applications are also presented. It is emphasized that succesful use of DNA
technology in a clinical laboratory requires well defined protocols,
high-quality and reliable procedure, proper education of laboratpry
professionals to perform the methods of recombinant DNA technology and
proper training of the physicians in the results interpretation.
Keywords: Isolation of nucleic acids, DNA, RNA, identification of DNA and RNA
- Type of paper
: Paper in proceedings
Title: Molecular diagnostics: practise, organization and the
quolity control
- Authors:
- Stavljenić-Rukavina, Ana
- Topić, Elizabeta (50266)
- Editors
- Salamuni', Ilza
- Dujmov, Ivana
Proceedings title: Molekularna biologija i primjena molekularno bioloŠkih metoda u klini;ko laborat
Language: hrvatski
Place: Split
Year: 1994
Pages: from 9 to 11
Meeting: 1. Stru;ni skup medicinskih biokemi;ara Hrvatske
Held: from 09/17/94 to 09/20/94
Summary: Molecular diagnisis has provided new possibilities in the
diagnosis and treatment of diseases from a wide spectrum of medical
specialties. Molecular diagnosis is defined as a new field of laboratory
medicine using nucleic acids as reagents in the analytical procedure and as
a sample which has to be analyzed. Molecular diagnosis techniques are
"molecular" techniques, as they rely on the primary structure of the
analyte, e.g., linear codes of the bases occurring in the analyte, the on
the total amount, size of ploidity, viscosity or base content. The in vitro
development of amplification techniques (PCR) has increased sensitivity of
the methods using DNA probes by 10 to 10.000 times, above the limits of
clinical laboratory techniques used to date.
Keywords: Molecular diagnostics, DNA-probe, PCR
- Type of paper
: Summary in proceedings
Title: Determination of bioactivity of hepatopoietin - a
hepatocyte proliferation factor
- Authors:
- Zadro, Renata (95160)
- Gluhak, Jelica (160474)
- Slijepčević, Milivoj (43700)
- Topić, Elizabeta (50266)
Proceedings title: DL Diagnostyka Laboratoryjna Abstracts
Language: engleski
Place: Krakow, PL
Year: 1991
Pages: from 176 to 176
Meeting: IX European Congress of Clinical Chemistry
Held: from 09/08/91 to 09/14/91
Summary: 50 h after partial hepatectomy, the rat remnant liver was
homogenized with water and hepatopoietin extracted by acidifying to pH 5.5
and boiling at 95 C. Further purification was done by chromatography on
Sephadex G-100 and 4 fractions within the range between 33000 and 43000 D
were collected. Bioactivity was tested on primary hepatocyte culture by
incorporation of 3H-thymidine into hepatocyte DNA. Results showed: (1) 2 to
3 fold increase in radioactive thymidine incorporation in DNA and 2)
partialpurification of rat liver hepatopoietin extract provided
hepatopoietin of a higher activity.
Keywords: hepatopoietin, Sephadex G-100 chromatography, hepatopoietin bioactivity, hepatocyte proliferation
- Type of paper
: Summary in proceedings
Title: A study of the effect of human hepatopoietin on hepatocyte
proliferation
- Authors:
- Topić, Elizabeta (50266)
- Zadro, Renata (95160)
- Gluhak, Jelica (160474)
- Slijepčević, Milivoj (43700)
Proceedings title: IX Europski kongres kliničke kemije, Eurolab 91
Language: engleski
Place: Krakow, PL
Year: 1991
Pages: from 177 to 177
Meeting: IX European Congress of Clinical Chemistry
Held: from 09/08/91 to 09/14/91
Summary: The proliferative activity of hepatopoietin extract
isolated from liver patients (n=29) was tested by inoculation of hepatocyte
and measurement of 3H thymidine incorporation into hepatocyte DNA. The mean
proliferative activity was 149% (range,96%-217%). Results of flow cytometry
revealed the cell cycle proceeding from phase G1 to phase S to differ
between hepatocytes inoculated with hepatopoietin (mean %Go/G1=73+2.93,
%S=20.8+4.96,and %G2+M=5.07) and those without inoculation
(mean%Go/G1=89.5+3.5, %S=10.5+2.8, and %G2+M=1.47+0.36).
Keywords: hepatopoietin, human hepatopoietin, hepatocyte proliferation, primary carcinoma, secondary carcinoma, chronic liver disease
- Type of paper
: Summary in proceedings
Title: Identification of hepatopoietin by means of SDS
electrophoresis
- Authors:
- Gluhak, Jelica (160474)
- Zadro, Renata (95160)
- Slijepčević, Milivoj (43700)
- Topić, Elizabeta (50266)
Proceedings title: IX Europoski kongres kliničke kemije
Language: engleski
Place: Krakow, PL
Year: 1991
Pages: from 176 to 176
Meeting: IX European Congress of Clinical Chemistry
Held: from 09/08/91 to 09/14/91
Summary: Hepatopoietin was isolated from rat liver remnant 50 h
after partial hepatectomy and further purified by gel filtration on
Sephadex G-100. Purified bioactive hepatopoietin fraction was the only
fraction containing proteins of about 40000 D and separated on SDS
electrophoresis according to Laemmli, produced a band at about 40.000 D.
The hepatopoietin extract isolated from blood ofliver patients, when
SDS-treated and separated according to its molecular weight, on SDS
electrophoresis showed the identical band as that of rat hepatopoietin.
Keywords: Hepatopoietin, SDS electrophoresis, gel filtration
- Type of paper
: Summary in proceedings
Title: Liver specific growth factor - hepatopoietin
- Authors:
- Topić, Elizabeta (50266)
- Zadro, Renata (95160)
- Paripović, Roberta
- Popović, Jasna
- Bjelinski, Dijana (177060)
- Slijepčević, Milivoj (43700)
Proceedings title: 2nd Alpe-Adria Congress on Clinical Chemistry and Laboratory Medicine, Abstracts
Language: engleski
Place: Graz, Austria
Year: 1992
Pages: from 86 to 86
Meeting: 2nd Alpe-Adria Congress on Clinical Chemistry and Laboratory Medicine
Held: from 11/12/92 to 11/14/92
Summary: Human plasma hepatopoietin (hPHP) extract was isolated from
81 liver patients and its proliferative activity was compared to the crude
rat liver hepatopoietin extract isolated 50h after partial hepatectomy. The
hPHP extract proliferative activity was 169% (115%-201%), 149.3%
(120%-172%) and 196%(152%-246%) for primary and secondary carcinoma, and
chronic liver disease, respectively, whereas the proliferative activity of
the rat hepatopoietin extract was 100%. Results of DNA flowcytometer
analysis showed an increase in cellular DNA content in the S phase of the
hepatocyte cell cycle too.
Keywords: Liver specific growth factor, hepatopoietin, proliferative activity, liver disease patients.
- Type of paper
: Summary in proceedings
Title: Evaluation of hepatopoietin in liver disease patients
- Authors:
- Topić, Elizabeta (50266)
- Barilar-Antoljak, Nataša (178140)
- Paripović, Roberta
- Bjelinski, Dijana (177060)
- Popović, Jasna
- Slijepčević, Milivoj (43700)
Proceedings title: Annales de Biologie Clinique
Language: engleski
Place: Nice, Francuska
Year: 1993
ISBN/ISSN: 003-3898
Pages: from 507 to 507
Meeting: Eurolab 93. 10th IFCC European Congress of Clinical Chemistry & 12th SFBC National Meeting
Held: from 04/25/93 to 04/29/93
Summary: Human plasma hepatopoietin (hPHP) extract was isolated from
38 liver patients. The localization of active hepatopoietin substance in
crude hPHP was done by SDS-PAGE. Electrophoretic pattern showed three
protein bands in the range of Mr 38000+/-5000, i.e. bands A, B, and C, Mr
about 41700, 38000, and 33800, respectively. 82% patients had positive band
A, 95% bandB, and 63% band C. In total, 97% of patients had one of the
protein bands positive. The bands were compared to the results on
biochemical parameters commonly used to test the liver function.
Keywords: hepatopoietin, human plasma hepatopoietin, SDS-PAGE, liver disease patients
- Type of paper
: Summary in proceedings
Title: Investigation of hepatopoietin in liver disease patients
- Authors:
- Topić, Elizabeta (50266)
- Barilar-Antoljak, Nataša (178140)
- Paripović, Roberta
- Bjelinski, Dijana (177060)
- Popović, Jasna
- Slijepčević, Milivoj (43700)
Proceedings title: The Clinical Biochemist-Reviews
Language: engleski
Place: Melbourne, Australia
Year: 1993
Pages: from 348 to 348
Meeting: IX European Congress of Clinical Chemistry
Held: from 11/14/93 to 11/19/93
Summary: From sera of 72 patients with liver disease, a crude
hepatopoietin extract was isolated and its protein bands were separated by
SDS-PAGE. Three protein bands in the range Mr of 38000+/-5000, i.e. A about
41700, B about 38000 and C about 33800, were compared to the results of
transaminases, alkalinephosphatase, GGT, CHE, LDH and total bilirubin. The
SDS-PAGE bands A, B, and C were found in 87.5%, 95.8% and 79.2% patients,
respectively. The frequency of hepatopoietin bands in patients with values
of biochemical parameters higher than 3SD of the mean ranged between
70%-100%.
Keywords: hepatopoietin, human hepatopoietin extract, SDS-PAGE, liver disease patients
- Type of paper
: Summary in proceedings
Title: Hepatopoietin a hepatocyte growth factor in liver
cirrhosis patients
- Authors:
- Topić, Elizabeta (50266)
- Paripović, Roberta (178746)
- Gluhak, Jelica (160474)
- Zadro, Renata (95160)
- Slijepčević, Milivoj (43700)
- Ruhenstroth-Bauer, Gerhard
- Editors
- Adlercreuz, Herman
- Penttila, Ilkka
- Tenhunen, Raimo
- Weber, Teddz
Proceedings title: Laboratory Medicine' 95,
Language: engleski
Place: Tampere, Finska
Year: 1995
Pages: from 182
Meeting: Eurolab 93. 10th IFCC European Congress of Clinical Chemistry & 12th SFBC National Meeting
Held: from 07/02/95 to 07/07/95
Summary: This study was undertaken to evaluate the
electrophoretically separated hepatopoietin bands in patients with liver
cirrhosis and compare them with biochemical markers of the liver state.
Human plasma hepatopoietin (hPHP) extract was isolated by the method of
Goldberg at all and Topi' et all, from 30 patients with confirmed liver
cirrhosis and 25 healthy volunteers. protein bands of the hepatopoietin
extract were electrophoretically separated by SDS-PAGE. The SDS-PAGE
elpherogram showed three protein bands, i.e. band A, B, and C, Mr of about
41.700, 38.000 and 33.800 respectively, in the range between Mr of 30.000
to 50.000. 92% of patients had positive bands A, 97% band B, and 85% band
C. In controls 80% had positive band A, 88% band B, and 80% band C.
Compared to the results on biochemical parameters the bands showed the
highest frequency of band A in patients with ALT and LDH values by 3 SD
above upper reference limit. The highest frequency of band B was found in
patients with TB, AP, AST, GGT, AFP and CHE values by 3SD higher than the
upper reference limit. The lowest frequency was observed with band C in
patients, regardless of the biochemical parameter evaluated.
Keywords: human plasma hepatopoietin extract, hPHP, SDS-PAGE, liver cirrhosis
- Type of paper
: Summary in proceedings
Title: Evaluating of hepatocyte growth factor in cirrhotic
patients
- Authors:
- Barilar-Antoljak, Nataša (178140)
- Topić, Elizabeta (50266)
- Paripović, Roberta (178746)
- Gluhak, Jelica (160474)
- Zadro, Renata (95160)
- Slijepčević, Milivoj (43700)
- Ruhenstroth-Bauer, Gerhard
- Editors
- Schmid, R.
- Bianchi, L.
- Blum, H.E.
- Gerok, W.
- Maier, K.P.
- Stadler, G.A.
Proceedings title: Abstracts X International Congress of Liver Diseases
Language: engleski
Place: Basel, švicarska
Year: 1995
Pages: from 300 to 300
Meeting: 10. internacionalni kongres o jetrenim bolestima. Acutne i kroni;ne jetrene bolesti: Molekularna biologija i klinika
Held: from 10/19/95 to 10/21/95
Summary: This study was undertaken to evaluate the
electrophoretically separated hepatopoietin protein bands in patients with
liver cirrhosis and compare them with the control group. The human plasma
hepatopoietin extracts were electrophoretically separated by SDS-PAGE
electrophorese. The electrophoretic pattern showed three protein bands in
the range between 30.000 and 50.000 D, named protein bands A, B, and C, of
about 41.700, 38.000 and 33.800 D respectively. In the cirrhosis group,
73.3% of patients had positive band A, 93.3% band B, and only 60% band C.
In controls, 80% of healthy volunteers had positive band A, 88% band B, and
80% band C. The results suggested impared balans between the factors with
proliferative and inhibitory activity.
Keywords: liver cirrhose, human plasma hepatopoietin, hepatopoietin protein bands, SDS-PAGE
- Type of paper
: Summary in proceedings
Title: A Computer expert program for clinical decision
- Authors:
- Topić, Elizabeta (50266)
- Petrove;ki, Miljenko
- Kljaji', Mirjana
- Ivanić, Davor (178500)
- Stavljenić-Rukavina, Ana
- Kvarantan, Marin
- GjuraŠin, Mirko
- Editors
- Robba, L.
- Martinelli, M.
Proceedings title: First Alpe-Adria Meeting on Clinical Chemistry and Laboratory Medicine
Language: engleski
Place: Gorizia, Italia
Year: 1991
Pages: from P25 to P25
Meeting: 1st Alpe-Adria Meeting on Clinical Chemistry and Laboratory Medicine
Held: from 03/01/91 to 03/02/91
Summary: Clinical decision making is a very complicated process, but
among the means available to assist in the process, the modern laboratory
is one that can contribute greatly to the abilities making the clinical
decision. In emergency laboratory, with a program of analysis covering the
needs of internal, pediatric, neurologic, urologic and surgery clinics, we
developed a sophisticated computer program, as an expert system allowing
clinical decision making on the basis of emergency laboratory findings. In
this program, analytes from five categories; electrolytes, metabolities,
enyzmes, hematology related tests and parameters of the acido-base balans
were combined so that their absolute values in time and their mutual
relationships presented panels for making diagnosis of different emergency
states caused by cardiac injury, hepatic or kidney injury or states related
to glucose metabolism disturbances.
Keywords: Expert systems, clinical decision, cardiac injury, hepatic failure, kidney injury, glucose disturbance
- Type of paper
: Ph.D.
Title: Isolation and characterization of hepatopoietin -
hepatocyte proliferative factor
Faculty: Farmaceutsko - biokemijski u Zagrebu
Date of defense: 07/10/92
Language: hrvatski
Number of pages: 144
Summary: A crude liver extract called hepatopoietin, isolated
fromregenerating rat livers and from rat plasma 50 h after
partialhepatectomy, induced a two-fold increase in DNA synthesis butonly in
the S phase of cell cycle, suggesting its proliferativeactivity. The
plasma of patients with primary and secondaryhepatocarcinoma and those with
chronic liver disease contained aliver growth promoting factor similar to
hepatopoietin. Humanplasma hepatopoietin showed the same properties but
inducedproliferation more effectively, i.e. 2.5 -3.5-fold, increasing
the cell cycle S phase too.
Keywords: Hepatopoietin, liver growth promoting factor, hepatocyte proliferation, DNA sinthesis
- Type of paper
: M.A.
Title: Effect of hepatopoietin on the enzyme activity in
hepatocytes
Faculty: Farmaceutsko - biokemijski u Zagrebu
Date of defense: 10/24/94
Language: hrvatski
Number of pages: 81
Summary: The extract of calf liver after partial hepatectomy was
analysed, presuming that it should have contained an active substance with
hepatopoietin-like action. Biologic activity of the calf hepatopoietin
extract (cHTP) was assessed by radioactive (3H-thymidine incorporation into
hepatocyte DNA) and non-radioactive method (BrDU incorporation into
hepatocyte DNA), which revealed the calf liver to contain an active
substance with hepatopoietin-like action. The highest biologic activity of
cHTP was shown in concentration of 0.1 ng/mL, which was ascertained by both
methods (p>0.05), suggesting the non-radioactive method, as a less risky
and adequatelt sensitive method, to be the method of choice for the study
of hepatocyte proliferation.
The biologic activity of cHTP was also confirmed by the determination of
the catalytic activities of the AST, ALT and LDH enzymesin primary
hepatoczte culture. cHTP was found to increase the catalytic concentrations
of all the three enzymes, among which AST showed a statistically
significant increase in the presence of an optimal concentration of cHTP.
With the use of SDS-PAGE, cHTP was found to contain three protein bands
identical to those observed in rat hepatopoietin in the region of molecular
masses of 41.700, 38.000 and 33.800 D.
Keywords: hepatopoietin, enzyme activity in hepatocyte, SDS-PAGE, BrDU-incorporation, hepatocyte proliferation
- Type of paper
: Mentorship
Title: Izolation and characterization of hepatopoietin -
hepatocyte growth factor
Faculty: Farmaceutsko - biokemijski u Zagrebu
Mentor: ADLER NEVENKA
Date of defense: 07/10/92
Number of pages: 144
Author: Zadro Renata
Degree level: Ph.D.
- Type of paper
: Mentorship
Title: Effect of hepatopoietin on enzyme activity in hepatocytes
Faculty: Farmaceutsko - biokemijski u Zagrebu
Mentor: TOPIĆ ELIZABETA
Date of defense: 10/24/94
Number of pages: 81
Author: Paripović Dipl ing medicinske biokemije Roberta
Degree level: M.A.
- Type of paper
: Mentorship
Title: Ethylenglycole-human control material
Faculty: Farmaceutsko - biokemijski u Zagrebu
Mentor: TOPIĆ ELIZABETA
Date of defense: 02/28/95
Number of pages: 102
Degree level: D.A.
- Type of paper
: Mentorship
Title: Preclinical, pharmaco-toxicological examination of
three-peptide Serine-Asparagine-Lysine
Faculty: Farmaceutsko - biokemijski u Zagrebu
Mentor: TOPIĆ ELIZABETA
Date of defense: 04/19/95
Number of pages: 38
Author: Balen Dipl ing farmacije Mirna
Degree level: D.A.
- Type of paper
: Mentorship
Title: Dry chemistry; laboratory and clinical evaluation of
bilirubin, total proteins, and activity of AST, ALT, ALP, and GGT
Faculty: Farmaceutsko - biokemijski u Zagrebu
Mentor: TOPIĆ ELIZABETA
Date of defense: 07/11/95
Number of pages: 66
Author: Benceti' Dipl.ing. medicinske biokemije Vlatka
Degree level: D.A.
- Type of paper
: Mentorship
Title: Glucose metabolism and intermediers of glucose in acute
cerebrovascular insult
Faculty: Medicinski fakultet Sveu;iliŠte u Zagrebu
Mentor: TOPIĆ ELIZABETA
Date of defense: 01/25/92
Number of pages: 156
Author: Pavli;ek Dr.sc.dr.med Ivan
Degree level: Ph.D.
- Type of paper
: Survey/Study
Title: Evaluation of the Kodak Ektachem 500 Dry Chemistry System
- Authors:
- Topić, Elizabeta (50266)
- Alpeza, Ines
- :unovi', Miljenka
- Barbari', Vesna
Ordering party: Zastupnik tvrtke Kodak, Meditrade-Zagreb
Institution depot: Zastupnik tvrtke Kodak, Meditrade-Zagreb
Year: 1993
Number of pages: 9
Language: engleski
Summary: Evaluation on Kodak Ektachem 500 dry chemitry system was
performed according to the protocol of the European Committee for Clinical
Laboratory Standards (ECCLS Document Vol. 3, No. 2, 1985). The analytical
system Ektachem 500 the multi chanel, discrete random access clinical
chemistry analyzer was evaluated for imprecision, accuracy, linearity,
sensitivity and specificity. Endogenous interferences (hemoglobin, protein
lipoprotein and bilirubin) as well as egsogenous interferences (therapeutic
drugs) were also considered. Practicability of the Ektachem 500 system in a
daily and busy clinical hospital emergency laboratory with a turnover of
about 2.000 analyses per 24 h was also assessed. Results statistically
evaluated by Student-t test, linear correlation and Passing-Babloch method
for comparison of analytical systems, showed that the system is precisse,
accure, sensitive and very specific and it is suitable for medical
laboratories who needs a rapid workflow a large number of samples.
Keywords: Ektachem 500, dry chemistry, Passing-Babloch, evaluation methods
- Type of paper
: Invited lecture
Title: Principle of dry chemistry in laboratory diagnostics
Institution: Hrvatsko druŠtvo medicinskih biokemi;ara
Year: 1993
- Type of paper
: Invited lecture
Title: Liver specific growth factor - hepatopoietin
Institution: 2. ALPE ADRIA Kongres
Year: 1992
- Type of paper
: Invited lecture
Title: Hepatopoietin - hepatocyte growth factor in liver
cirrhosis patients
Institution: 11. Europski kongres klini;ke kemije
Year: 1995
- Type of paper
: Other
Title: Methods of molecular biotechnology in clinical laboratory
- Authors:
- Stavljenić-Rukavina, Ana
- Topić, Elizabeta (50266)
- Barišić, Karmela
Type of work: Priručnik
Language: hrvatski
Summary: The handbook contains contributions providing
generalintroduction into the biochemistry of nucleic acids, followed
bypresentation of the main techniques of molecular biotechnologyadopted and
practised by the authors. Experimental models usingthese methods in the
investigation of a gene disorders aredescribed. A number of highly useful
contributions are found inthe appendices, i.e.: lists of equipment,
accessories and workingconditions, and a glossary of molecular
biotechnology which willprobably be found valuable for better mutual
understandingbetween the researchers and professionals.
Keywords: Molecular biotechnology, nucleic acids, PCR, DNA isolation, RNA isolation, cloning, sequencing, electrophoresis
