- Type of paper
: Paper in journal
Title: MORPHOHISTOCHEMICAL AND EMG OBSERVATIONS OF MUSCULUS
BICEPS BRACHII IN HUMANS OF VARIOUS LEVELS OF PHYSICAL ACTIVITY
- Authors:
- Medved, Vladimir (88491)
- Bobinac, Dragica (82736)
- Šentija, Davor (160160)
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 87 to 90
Number of references: 10
Language: engleski
Summary: Skeletal muscles are the generators of active force and
therefore the actuators in the locomotor system. Muscle tissue, namely, is
characterized by properties like excitability, conductivity, elasticity and
above all contractility, enabling the muscle the role of actuator. When
researching muscle and muscular system it is important to explore the
relation between the muscle's structure and function. We have pursued a
study along these lines by providing morphohistochemical analysis of human
muscle biceps brachii and electromyography (EMG) and dynamometry
measurements of actively contracting muscle.
Plotting the histograms of occurence of fiber diameters, at the scale of 3
micro meters (um) resolution, reveals that in younger persons the biceps
brachii specimen for ATP-ase activity at pH 4.3 show a greater frequency of
type II fibers with a diameter from 91 to 106 um, while type I fibers have
the highest frequency of occurence at diameters ranging from 22 to 28 um.
In older individuals the type II fibers are smaller with the highest
frequency of fibers with diameters ranging from 61 to 79 um; the type I
fibers are larger, with the highest frequency of fibers with diameters
ranging from 58 to 76 um.
Despite significantly different levels of maximum isometric force developed
by our subjects - corresponding to their physical abilities - in all cases
analyzed a proportionality between measured force and smoothed EMG values
may be observed. The current level of scientific knowledge suggests with
high probability, the existence, in subjects manifesting higher isometric
force gradients, of a higher proportion of type II fibers characterized by
higher contraction strength.
Keywords: Human m. biceps brachii, myosin ATP-ase, EMG, dynamometry.
- Type of paper
: Paper in journal
Title: HISTOCHEMICAL ANALYSIS OF M. SOLEUS AND M. TA DENERVATED
BY DIFFERENT METHODS OF DENERVATION ON N. ISCHIADICUS
- Authors:
- Malnar-Dragojević, Danijela (134611)
- Stančić, Marin
- Bobinac, Dragica (82736)
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 81 to 82
Number of references: 5
Language: engleski
Summary: The aim of this study was to determine whether injuries of
the N. ischiadicus of IV and V degrees (by Sunderland) provoke differences
in the growth rate of nervous fibers, and changes in types of muscular
fibers in 2-month old AO rats. The IV degree injuri was done by nerve
compression with microneedleholder for one minute. While the V degree
injuri was done by transection of the N. ischiadicus in the other group.
By observing the muscle weight, it was established that in the first group
of animals subjected to compression of N. ischiadicus, the muscle weight
decreased until the 17th day when there was no further decrease of muscle
weight . Muscle weight continually decreased until the end of the
experiment in the second group of animals with V degree injuri.
Histological examination of nerves, established that the distal axons in
both cases were affected by Wallerian degeneration. The IV degree injuri
left the epineurium intact, unlike the V degree injuri which damaged it.
The initial signs of regeneration of the nervous fibers were presented in
both ways of denervation, compression and transection alike.
Muscle fibers of M. soleus and M. tibialis anterior were typified using the
method for demonstration of myosin ATP-ase activity. In M. soleus,
characterized by prevalence of type I fibers, the number of type II fibers
gradually increased. Regarding ATP-ase activity, we established that
changed muscle fiber types in soleus muscle were presented in both types of
denervation, indicating type I fibers to be under greater neural control
than type II muscle fibers.
Keywords: Nerve compression and transection, muscle mass, muscle fibre type I and II, nerve regeneration.
- Type of paper
: Paper in journal
Title: INFLUENCE OF REPEATED TONIC-CLONAL CONVULSIONS ON THE DEEP
DORSAL MUSCLE AND DIAPHRAGM OF THE RAT
- Authors:
- Bobinac, Dragica (82736)
- Matešić, Damir
- Malnar-Dragojević, Danijela (134611)
- Jerković, Romana (196103)
- Šoić, Tamara (195622)
- Simonić, Ante (43202)
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 83 to 84
Number of references: 3
Language: engleski
Summary: By chronic electrical stimulation elicited changes are
different, depending on frequencies, regularity and duration of the
stimulation. Mainly, the results show a fast to slow fiber transformation.
We were interested in how pathological conditions of repeated tonic-clonal
covulsions influence to morphohistochemical characteristics of the deep
dorsal muscle (DD) and diaphragm.
Type I and II fibers were examined by myosin ATP-ase method. The connective
tissue of the endomysium and perimysium was stained by the
Weigert-elastica-Van gieson method. Female rats were stimulated with
electrodes attached to the ears for 5, 10 or 15 days. The applied stimulus
was 75 mA, 50 Hz, lasting for 1 second. Such stimulation produced
tonic-clonal convulsions lasting 12-15 seconds and opistotonus for 7-10
seconds.
Type II fiber predominated in medial and lateral part of the DD, and in the
lateral part type I fiber is less than in the medial part. A 5- or 10-day
stimulation does not induce changes in distribution of fiber types in the
DD and diaphragm, but induces a decrease of oxidative capacity of type I
fiber and their number. A 15-day stimulation induces a decreased of the
number of type I fibers in most parts of the cross section area but at some
points can be seen transformation type II to type I fibers. The endomysium
is visible and thick.
Keywords: Deep dorsal muscle, diaphragm, electrical stimulation, muscle fibers type I and II, connective tissue.
- Type of paper
: Paper in journal
Title: THE ACTIVITY OF NADH-TR AND ALFA-GPDH IN THE HUMAN
MULTIFIDUS MUSCLE
- Authors:
- Bajek, Snježana (134622)
- Bobinac, Dragica (82736)
- Bego, Uroš
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 85 to 86
Number of references: 4
Language: engleski
Summary: Oxidative and glycolytic capacity of muscle can indicate
its functional properties and can also be presented by
enzyme-histochemical methods. This analysis was carried out on samples of
36 human multifidus muscles taken at obduction from the level at L4, in
persons who died of sudden death aged from 18-50 years.
The type I fibers, making up 61.52% of all the fibers, show stronger
oxidative capacity, while the activity of NADH-TR in the type II fibers is
weaker. The type II fibers demonstrate a stronger glycolytic capacity than
the type I fibers. Only a part of type I fibers in some samples shows a
somewhat stronger activity of alfa-GPDH, but weaker than that of the type
II fibers. In a few samples one part of the type I fibers shows a somewhat
weaker NADH-TR activity than the other type I fibers in the same sample,
but is stronger than that of type II fibers. There are no type II fibers
with a stronger oxidative capacity than that of the type I fibers.
Keywords: M. multifidus, oxidative and glycolytic enzymes.
- Type of paper
: Paper in journal
Title: DIFFERENCES IN METABOLIC ACTIVITY IN MUSCLE FIBRES OF THE
CATFISH (SCYLLUM CANICULA CUV.) AND SARDELLE (CLUPEA SPRATTUS L.)
- Authors:
- Pernjak-Pugel, Ester (133643)
- Bobinac, Dragica (82736)
- Tomac, Jelena (133676)
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 75 to 76
Number of references: 2
Language: engleski
Summary: The aim of this study was to investigate the metabolic
activity of axial sceletal muscle fibres in two types of telost from the
Adriatic Sea - the catfish (Scyllum canicula cuv.) and the sardelle (Clupea
sprattus L.) using the following histochemical techniques.
The NADH-TR method by Spanhoff is used to prove enzymatic activity of the
respiratory chain. The alfa-GPDH method by Weiner shows the intensity of
enzyme activity of glycolysis. In applying the PAS method for
carbohydrates, and Sudan black B for lipids we can also notice differences
which depend on the type of muscle fibres.
Our results show a high activity of the Krebs' cycle enzyme (NADH-TR) in
type I fibers. This type are fibres of small diameter with a well developed
endomysium, localized laterally, below the skin in both species. The same
applies to results obtained by the PAS and Sudan black B methods, which
indicate higher levels of carbohydrates and lipids in type I fibers in
sardelle, as compared to those in the catfish. Type II fibers form the main
body mass in both species. These fibers have wite diameter, with a poorly
developed endomysium around them. They are NADH-TR negative and alfa-GPDH
positive.
Keywords: Muscle fiber type I and II, enzymatic activity, NADH-TR, alfa-GPDH, PAS, Sudan black B.
- Type of paper
: Paper in journal
Title: FUNKCIONALNA RAZDIOBA VLAKANA M. BICEPS BRACHII ČOVJEKA
- Authors:
- Bobinac, Dragica (82736)
- Bego, Uroš
- Šentija, Davor (160160)
Journal: Medicina
Number: 3-4
ISSN: 0025-7729
Volume: 29
Year: 1993
Pages: from 103 to 106
Number of references: 21
Language: engleski
Summary: The fiber diameter, as one of the morphometric parameters,
is mentioned in respect to fiber types. Thus, the diameter of type I fibers
is smaller and of type II is greater, although it is evident that the
diameters of both muscles fibers differ considerably within a group.
Therefore, the study was focused on the distribution of all muscle fibers
in respect of their diameter, separately for both types.
On muscle samples of the biceps brachii obtained from six healthy persons
aged 20-40 yr, quantitative and histochemical analysis was performed.
Considering the myosin ATP-ase activity (pH 4.3) fibers were divided into
dark (type I) and light (type II). Their number was determined and the
diameters measured.
The distribution frequency of all dark and light fibers diameter with a
step of 3 um shows that there are 41 morphofunctional units (MFUs) in the
biceps brachii, indicating their great heterogeneity. The presence of dark
fibers of small diameter (22-31 um) is marked (28%), cleary indicating
their frequent activity at arm movement with small muscle force, while
fibers of greater diameter, being dark or light, are successively activited
when greater or great muscle force is required.
The distribution frequency of both fiber types in respect of their
diameter, demonstrates marked heterogeneity of fibers and plasticity of
there function.
Keywords: M. biceps brachii, fiber heterogeneity, fiber diameter distribution frequency, morphofunctional units (MFU).
- Type of paper
: Paper in proceedings
Title: UTJECAJ MAGNETSKE STIMULACIJE NA DENERVIRANE SKELETNE
MIŠIĆE
- Authors:
- Bobinac, Dragica (82736)
- Schnurrer, Tea (196125)
- Grubješić, Igor (200132)
- Malnar-Dragojević, Danijela (134611)
Proceedings title: CROMBES & KOREMA Proceedings (1994)
Language: engleski
Place: Zagreb
Year: 1994
ISBN/ISSN: 953-6037-06-8
Pages: from 50 to 53
Meeting: 10th INTERNATIONAL SYMPOSIUM ON BIOMEDICAL ENGINEERING '94
Held: from 11/24/94 to 11/26/94
Summary: Male rats, y-line were treated 10 days by time-varying,
pulse, low frequency, magnetic field. We wanted to fortify the influence of
magnetic field density 10 mT, with frequency of 10 Hz, 10 minutes daily,
through 10 days continuously, on denervated skeletal muscles. Denervation
was performed by compression of sciatic nerve and after that rats were
stimulated. On transverse sections of soleus and tibialis anterior muscles
we wre represented the types of muscle fibre and capillaries by
histochemical methods.
The biggest change in stimulated muscles was increasing the number of
capillaries. That number is greater in healthy or denervated muscles which
were stimulated, than in healthy or denervated controls. Measuring the
muscle mass we noticed decreasing the percentage of atrophy in stimulated
muscles after 18 days of denervation.
Keywords: Muscle mass, number of capillaries, magnetic stimulation, denervation.
- Type of paper
: Paper in proceedings
Title:
- Type of paper
: Summary in proceedings
Title: UTJECAJ PONAVLJANIH TONIČKO-KLONIČKIH KONVULZIJA NA DUBOKU
LEĐNU MUSKULATURU
- Authors:
- Bobinac, Dragica (82736)
- Matešić, Damir
- Malnar-Dragojević, Danijela (134611)
- Jerković, Romana (196103)
- Šoić, Tamara (195622)
- Simonić, Ante (43202)
Proceedings title: Abstracts
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 52 to 0
Meeting: 16th ALPS ADRIA MEETING OF ANATOMISTS
Held: from 05/20/93 to 05/23/93
Summary: By electrical stimulation (75mA, 50Hz, lasting 1sec)
wereproduced tonic-clonal convulsions with expressed opistotonus.Such
stimulation was repeated daily in the course of 5, 10 or 15days. Serial
sections were stained for myosin ATP-ase at ph 9.4and 4.3, with NADH-TR and
alfaGPDH. Type II fibers predominate indeep dorsal muscle (DD) while fibers
type I are rare and single.But most of the fibers are positiv with NADH-TR
method what isnot in correlation with results of mATP-ase activity.
Percentageof both fiber types is similar in diaphragm. This
stimulationproduce decreasing of number of fibers type I and decline
fiberoxidative activity. Conversion and clustering of fibers type I isseen
after 15 days at some places in DD muscle. Endomysium andperimysium are
more thicker.
Keywords: Deep dorsal muscle, Diaphragm, Electrical stimulation.
- Type of paper
: Summary in proceedings
Title: HISTOKEMIJSKA ANALIZA M.SOLEUS I M.TA NAKON RAZLIČITIH
METODA DENERVACIJE N.ISCHIADICUS
- Authors:
- Malnar-Dragojević, Danijela (134611)
- Stančić, Marin
- Editors
- Malnar-Dragojević, Danijela (134611)
- Stančić, Marin
- Bobinac, Dragica (82736)
Proceedings title: Abstracts
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 51 to 0
Meeting: 16th ALPS ADRIA MEETING OF ANATOMISTS
Held: from 05/20/93 to 05/23/93
Summary: It was done the injury of IV degree by compression
ofn.ischiadicus by microneedleholder, lasting one minute and injuryof V
degree by transection of the nerv. After compression andtransection atrophy
is presented in both muscles with greaterchanges in m.soleus. In m.soleus,
which is characterised byprevalence of type I fibres, the number of type II
fibresgradually increased as result of denervation. After compressionmuscle
weight decreased till the 17th day with no furtherdecrease of muscle
weight. In the second group of animals withinjury of V degree weight of
muscle continually decreased tillthe end of the experiment.
Keywords: M.soleus, M.tibialis anterior, Denervated muscle, Muscle fiber types, Denervated n.ischiadicus
- Type of paper
: Summary in proceedings
Title: AKTIVNOST NADH-TR I alfaGPDH U M. MULTIFIDUS ČOVJEKA
- Authors:
- Bajek, Snježana (134622)
- Bobinac, Dragica (82736)
Proceedings title: Abstracts
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 54 to 0
Meeting: 16th ALPS ADRIA MEETING OF ANATOMISTS
Held: from 05/20/93 to 05/23/93
Summary: It was investigated metabolic activity of muscle fibers in
humanm.multifidus. The activity of NADH-TR and alfaGPDH is differentin both
muscle types what is in correlation withdifferentiation in type I and type
II. The type I fibers showstronger oxidative capacity while the activity of
NADH-TR in typeII fibers is weaker. Type II fibers demonstrate
strongerglycolytic capacity than type I fibers.
Keywords: M. multifidus, NADH-TR, alfaGPDH, Metabolic activity of muscle fibres.
- Type of paper
: Summary in proceedings
Title: HISTOKEMIJSKA ANALIZA TIPOVA MIŠIĆNIH VLAKANA U M.
MULTIFIDUS I M. LONGISSIMUS DORSI LUMBALNOG DIJELA U ČOVJEKA
- Authors:
- Bajek, Snježana (134622)
- Bobinac, Dragica (82736)
- Fužinac, Ariana (142213)
Proceedings title: Abstracts
Language: engleski
Place: Ljubljana, Slovenija
Year: 1992
Pages: from 9 to 0
Meeting: 5TH MEMORIAL MEETING NAMED AFTER THE LATE PROFESSOR DR. FRAN I. ZAVRNIK
Held: from 04/02/92 to 04/04/92
Summary: Muscle samples of m.multifidus end m.longissimus were taken
inthe level of 4th lumbar vertebra from persons aged from 18 till60 years.
On transverse serial sections distribution of musclefiber types was
reaalized by method of myosin ATP-ase at pH 9.4.In m.longissimus percentage
of fibers type I is greater than inm.multifidus but both muscles contain
greater number of fiberstype I than type II. The diameter of fibers type I
issignificantly greater than that of fibers type II.
Keywords: Lumbar part of m.multifidus, M.longissimus, Myosin ATP-asa, Muscle fibre types
- Type of paper
: Summary in proceedings
Title: RAZVITAK ATP-azne AKTIVNOSTI DUBOKIH MIŠIĆA LEĐA U ČOVJEKA
- Authors:
- Fužinac, Ariana (142213)
- Bobinac, Dragica (82736)
- Bajek, Snježana (134622)
Proceedings title: Abstracts
Language: engleski
Place: Ljubljana, Slovenija
Year: 1992
Pages: from 27 to 0
Meeting: 5TH MEMORIAL MEETING NAMED AFTER THE LATE PROFESSOR DR. FRAN I. ZAVRNIK
Held: from 04/02/92 to 04/04/92
Summary: We investigated the development of muscle fiber types in
fetaland newborn m.erector sspinae by method for myosin ATP-ase. Theresults
show that all fibers are undifferentiated IIC fibersuntil 20. week of
gestation. Until 30. week appear big, singlefibers type I surrounding with
little undifferentiated fibers.After 30. week the number of fibers type I
increases, otherfibers are differentiated as type II and a small number of
stillundifferentiated fibers are presented. During first year of
lifedifferentiation of fibers type II is going on.
Keywords: M.erector spinae, Development of ATP-ase activity, Myosin ATP-ase, Muscle fiber types
- Type of paper
: Summary in proceedings
Title: ANALYSIS OF PARAVERTEBRAL MUSCLES APPLYING COMPUTERIZED
TOMOGRAPHY IN PATIENTS WITH LOW BACK PAIN
- Authors:
- Halaji-Laaby, Adelaida
- Bobinac, Dragica (82736)
- Bajek, Snježana (134622)
- Bajek, Goran
- Editors
- Lovasić, Ivan
Proceedings title: Dijagnostička i intervencijska radiologija
Language: hrvatski
Place: Opatija
Year: 1994
ISBN/ISSN: 0
Pages: from 210 to 211
Meeting: PRVI KONGRES HRVATSKOG DRUŠTVA RADIOLOGA
Held: from 10/11/94 to 10/15/94
Summary: Paravertebral muscles are one of the very important factors
in maintaining the vertebral function. In the patients with low back pain
their role is even more important because serve as compensatory mechanism.
We analysed the density and the area of cross sections of paravertebral
muscles at the level of the intervertebral spaces L4/L5 and L5/S1. We
correlated our results with age and pathological changes of the examined
vertebral segment.In analysis of the involutiv muscle changes degree, we
concluded how is very important to include the fisical activity of the
patient before the illness and for how long time the symptoms are
presented. So the results showed that muscle changes are more dependent of
muscle fisical activity before the illnes and the lasting of symptoms which
couse the changing vertebral motility, than of the pathological changes of
the vertebral segment.
Keywords: Paravertebral muscles, CT, muscle density and cross section area
- Type of paper
: Summary in proceedings
Title: ELEKTROMIOGRAFSKA USPOREDBA REZULTATA ŽIVČANOG GRAFTA
UČINJENOG POMOĆU LUPE I MIKROSKOPA
- Authors:
- Stančić, Marin
- Fužinac, Ariana (142213)
- Bobinac, Dragica (82736)
- Editors
- Ribarič, Samo
- Zorec, Robert
Proceedings title: Book of abstracts
Language: engleski
Place: Ljubljana, Slovenija
Year: 1995
ISBN/ISSN: 961-90027-1-7
Pages: from 167
Meeting: LIFE SCIENCES 1995., International Conference
Held: from 09/23/95 to 09/28/95
Summary: Controversy surrounds the use of optic magnification for
peripheral nerve surgery. The aim of this study was to distinguish the
eventual differences in regeneration, measured as motor nerve conduction
velocity (MNCV), compound muscle action potential (CMAP) amplitude and the
clinical test equivalent (CTE), of loupe magnification vs. microscope
assistance tibial nerve grafts. Four groups of 12 animals in each, were
operated upon with 2.5 x, 5 x (loupe), 16 x and 40 x (microscop)
magnification. The recovery results were assessed by 2 independent
evaluators 5 months later. MNCV measured on the operated nerve was
expressed as a percentage of the normal side, while CMAP wave lenght,
latency period, amplitude and results of CTE were presented as arithmetic
mean. In each animal CMAP wave was biphasic, spread over 2.42+_1.08 msec,
with latency period of l.78+_0.70 msec.
MNCV in the group operated with loupe magnification and microscope
assistance was 26.77+_9.37 m/sec and 44.19+_11.36 m/sec respectively. The
same scores expressed as a percentage of the normal side are 39.37% and
63.90% (p<0.05). CMAP amplitude was 5.53+_2.78 mV in the loupe group and
7.01+_2.24 mV in microscope group (p<0.05). CTE was 2.0/3 in loupe and
2.8/3 in microscope group (p<0.01). Our results suggest the following:
- regeneration plateau has been reached as indicated by CMAP wave
pattern, length and distal motor latency period
- the fiber diameter is higher in the microscope group as indicated by
difference in MNCV
- the CTE difference confirms better motor-unit reinnervation coordinated
by cortically integrated sensory feedback in the microscope group again.
Keywords: Nerve graft, magnification, motor nerve conduction velocity, action potential, clinical test.
- Type of paper
: M.A.
Title: MORPHOHISTOCHEMICAL ANALYSIS OF HUMAN DORSAL MUSCLES
Faculty: Medicinski fakultet Sveučilište u Rijeci
Date of defense: 07/15/92
Language: hrvatski
Number of pages: 90
Summary: Due to various descriptions of deep dorsal muscles in
literature,gross dissection was done on embalmed cadavers in order
toestablish relations between these muscles as well as all otheranatomical
characteristics.
The lumbar parts of m.multifidus and m.longissimus thoracistaken from the
level of the fourth lumbar vertebra were alsohistochemicaly analysed.
The results show a predominance of type I fibers in both muscles.The
diameter of these fibers is significantly greater than thatof type II
fibers. The glycolytic capacity is strong in type IIfibers while oxydative
capacity is strong in type I fibers.The predominance of type I fibers,
strong oxydative capacity inthose fibres and their greater diameter as
compared to type IIfibers, point to the permanent role of those both
muscles inmaintaining the lumbar lordosis, i.e. their primarily
tonicactivity.
Keywords: M. multifidus, M. longissimus, Myosin ATP-ase, NADH-TR, alfaGPDH, Muscle fiber types, Metabolic activity, Fibre diameter.
- Type of paper
: M.A.
Title: FETAL AND POSTNATAL DEVELOPMENT AND DIFERENTIATION OF
MUSCLE FIBER TYPES IN LUMBAR PART OF M. ERECTOR SPINAE
Faculty: Medicinski fakultet Sveučilište u Rijeci
Date of defense: 07/15/92
Language: hrvatski
Number of pages: 86
Summary: The differentiation of fibre types in m. erector spinae of
fetusand newborns was studied. The enzymes activity of mATP-ase showedonly
the undifferentiated type IIC fibers before the 20th week ofgestation.
Before the 30th week, scattered large type I fiberswere differentiated.
After the 30th week fibres type II werepresented. At birth, we found a
similar percentage of fibres typeI and type II with still a significant
great number ofundifferentiated type IIC fibres. During the first year of
lifedifferentiation of fibers type II was going on.
The activity of oxidative and glycolytic enzymes was very weakduring fetal
development. Not earlier than last weeks of fetallife metabolic activity
was little more expressed but notsufficient to express the metabolic
profile of the muscle fibers.
Keywords: M. erector spinae, Development of enzymatic activity, Muscle fiber types.
- Type of paper
: M.A.
Title: THE EGZOGENE GENES EXPRESSION IN REGENERATING RAT SKELETAL
MUSCLES
Faculty: Medicinski fakultet Sveučilište u Rijeci
Author: JERKOVIĆ ROMANA
Date of defense: 12/23/94
Language: hrvatski
Number of pages: 72
Summary: Skeletal muscle can be transfected in vivo with plasmid
desoxyribonucleic acid, but the efficiency of gene transfer is relatively
low. We have compared the efficiency of direct gene transfer in normal and
regenerating rat skeletal muscle. The muscle necrosis and regeneration was
induced by an intramuscular injection of bupivacaine in the soleus muscle
of adult rats. Plasmids containing beta-galactosidase or chloramphenicol
acetyl transferase genes, driven by viral promoters were injected three
days after bupivacaine treatment into the regenerating and the
contralateral uninjured muscles. The expression of cloramphenicol acetyl
transferase activity was 87-fold higher in regenerating, compared to
control muscles 7 days after the transfection, but decreased at 30th and
60th day. The Southern blot analysis showed that the predominant form of
cloramphenicol acetyl transferase desoxyribonucleic acid in transfected
muscles was episomal; however, cloramphenicol acetyl transferase activity
measurements, performed on the same transfected muscles, showed no
correlation between the enzymatic activity and the amount of plasmid
desoxyribonucleic acid. The expression of beta-galactosidase was detected
in numerous regenerating fibres of the injured soleus muscles at the 7th
day post-transfection. In contrast, only few positive fibers were found in
control muscles. Focal infiltrates of the mononuclear cells, surrounding
and invading selectively beta-galactosidase positive fibers, were observed
at 30th day post-transfecton. This may suggest that an immune mechanism is
implicated in the progressive loss of transgenes over time. The finding
that regenerating muscle fibers display a higher efficiency of transfection
may be relevant to gene therapy of Duchenne muscular dystrophy, since
regenerating fibers are numerous in the early stages of the disease.
Keywords: Regenerating skeletal muscle, gene transfer in vivo, efficiency of gene transfer.
- Type of paper
: Mentorship
Title: FETAL AND POSTNATAL DEVELPOMENT AND DIFFERENTIATION OF
MUSCLE FIBER TYPES IN LUMBAR M. ERECTOR SPINAE
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: BOBINAC DRAGICA
Date of defense: 07/15/92
Number of pages: 86
Author: Fužinac mr.sc.,dr.med. Ariana
Degree level: M.A.
- Type of paper
: Mentorship
Title: MORPHOHISTOCHEMICAL ANALYSIS OF HUMAN DEEP DORSAL MUSCLES
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: BOBINAC DRAGICA
Date of defense: 07/15/92
Number of pages: 90
Author: Bajek mr.sc.,dr.med. Snježana
Degree level: M.A.
- Type of paper
: Mentorship
Title: THE EGZOGENE GENES EXPRESSION IN REGENERATING RAT SKELETAL
MUSCLES
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: BOBINAC DRAGICA
Date of defense: 12/23/94
Number of pages: 72
Author: Jerković dr. med. Romana
Degree level: M.A.
- Type of paper
: Mentorship
Title: THE INFLUENCE OF TIME-VARYNG, LOW FREQUENCY MAGNETIC FIELD
ON RAT DENERVATED SKELETAL MUSCLES
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: BOBINAC DRAGICA
Author: Grubješić dr.med. Igor
Degree level: M.A.
- Type of paper
: Mentorship
Title: ELECTROMIOGRAPHICAL EXAMINATION OF PARAVERTEBRAL MUSCLES
IN PATIENTS WITH LOW BACK PAIN
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: JURJEVIĆ ANTE
Author: Schnurrer dr.med. Tea
Degree level: M.A.
- Type of paper
: Mentorship
Title: MORPHOHISTOCEMICAL EXAMINATION OF LUMBAR MUSCLES SAMPLES
FROM THE PATIENTS OPERATED ON DISK HERNIA
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: BOBINAC DRAGICA
Author: Bajek mr.sc.,dr.med. Snježana
Degree level: Ph.D.
- Type of paper
: Mentorship
Title: THE REGULATION OF LAIT CHAINS MYOSIN GENE EXPRESSION OF
SLOW TYPE IN REGENERATING SKELETAL MUSCLE
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: BOBINAC DRAGICA
Author: Jerković dr. med. Romana
Degree level: Ph.D.
- Type of paper
: Mentorship
Title: OPTIC MAGNIFICATION AND RAT TIBIAL NERVE REGENERATION
AFTER NERVE GRAFT IMPLANTATION
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: BOBINAC DRAGICA
Author: Stančić mr.sc.,dr.med. Marin
Degree level: Ph.D.
- Type of paper
: Mentorship
Title: THE INFLUENCE OF BLOOD FLOW TO THE DEVELOPMENT OF ATROPHY
IN DENERVATED RAT SKELETAL MUSCLE
Faculty: Medicinski fakultet Sveučilište u Rijeci
Mentor: BOBINAC DRAGICA
Author: Malnar-Dragojević mr.sc.,dr.med. Danijela
Degree level: Ph.D.
