SVIBOR - Project code: 3-01-429

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

SVIBOR - Collecting Data on Projects in Croatia

Project code: 3-01-429


THE INFLUENCE OF AGE AND VERTEBRAL MOTILITY TO CHARACTERISTICS OF DEEP DORSAL MUSCLES


Main researcher: BOBINAC, DRAGICA (82736)


Assistants
Type of research: basic
Duration from: 10/10/91. to 10/10/94.
Papers on project (total): 27
Institution name: Medicinski fakultet, Rijeka (62)
Department/Institute: DEPARTMENT OF ANATOMY
Address: Braće Branchetta 20
City: 51000 - Rijeka, Croatia
Communication
Phone: 385 (0)51 227052
Fax: 385 (0)51 514915
Summary: We examined anatomical characteristics of the lumbar part of deep dorsal muscle on 30 cadavers of adult persons of both sexes. We found out that there is no clear demarcation between m.multifidus and m.longissimus which are composed of 5 bundles with different length, width and arrangement of muscle fibers what was precisely described. We established that each bundle is inervated by dorsal branch of lumbar spinal nerv what dispute affirmation that these muscles are polisegmentaly inervated. Histochemical methods for myosin ATP-ase activity, NADH-TR and alfaGPDH were used for demonstration of muscle fiber types. In m.multifidus and m.longissimus the greater number of fibers type I is presented. The diameter of fiber type I is greater than that of fibers type II what is specific result for these muscles. Fibers type I also have stronger oxidative activity what means very important static and permanent acitivity of these muscles. During fetal life differentiation of muscle fiber types according to enzymatic activity begins after 20th week of gestation. In the first place fibers type I appear. After 30th week fibers type II appear with still great number of undifferentiated fibers. At birth the number of fibers type I and II is similar and begin the development of their metabolic capacity . We made morphohistochemical analysis of muscle tissue taken from operated persons because of herniated disc. The changes were very similar to muscle denervation such as atrophy of fibers type I, increasing of the number of fibers type II,endomysium and perimysium thicken. We examined the patients with herniated disc and low back pain by computerised tomography. It was measured a density and cross section area of paravertebral muscles and results showed that density decreased because of increasing the quantity of connective and fat tissue, and cross section area was decreasing depending on duration of illness and phisical activity before the first symptoms. On the experimental animals we examined the influence of alternating,low frequency electromagnetic field to denervated skeletal muscles. After 10 days of stimulation the number of capillaries around the muscle fibers was significantly increased.

Keywords: Anatomy of lumbal part of deep dorsal muscle, muscle fiber types, fetal differentiation of fibers, muscle changes effected by herniated disc, electromagnetic stimulation.

Research goals: To determine the anatomical characteristics and interrelation between m.multifidus, m.longissimus and iliocostalis in lumbar part because the data, found in literature, are different. Good knowledge about muscle morphology such as position, width and length of bundles is necessary for correct and successful measuring of EMG activity. To examine EMG activity of all three muscles in healthy people of different age in order to study their function and degree of activity during different movements. To examine EMG activity of the same muscles in people with different degree of disarrangement or disability of lumbar spine for establishing the changes in the muscle function in order to correlate them with histochemical profile of muscle fiber types.The aim of this study is to find out if exists the correlation between those two parameters. To establish histochemical differentiation of muscle fibers type we use the method of myosin ATP-ase pH 4.3 and pH 9.4 and enzymes NADH-TR and alfa-GPDH. With these methods is expressed histochemical profile of examined muscles. As the derangement of the lumbar spine provokes disarrangement of muscle inervation, we tried on the model of denervated muscle to examine the influence of Salbutamol, agonist of beta-receptors, as muscle protector during the period of denervation. From the literature we know that some other agonists of beta-receptors provoke hipertrophy of the skeletal muscles. On the same model we also examine the influence of Nifedipin and Nimodipin as Ca antagonists. As we know that in phisical therapy during muscle denervation is using electrical stimulation, we will stimulate the muscles by electromagnetic field with determined density and frequency. The aim of this project is also to establish the methods of gene transfer in vivo. We would like to examine the specific gene expression for slow and fast muscle fiber types in regenerating skeletal muscle.

COOPERATION - INSTITUTIONS


  1. Name of institution: DIPARTIMENTO DI SCIENZE BIOMEDICHE SPERIMENTALI
    Type of institution: University/Faculty
    Type of cooperation: Joint publishing of scientific papers
    City: 35121 - Padova, Italija
Other information about the project.
MZT Croatian language SVIBOR Alphabetic list Sorted on project code Sorted on institutions Search help
Ministry of
Science and
Technology		 Croatian
language		 Svibor
homepage		 Alphabetic
list		 Sorted on
project code		 Sorted on
institutions		 Search Help
Last update: 12/04/95
Information: svibor@znanost.hr