- Type of paper
: Paper in journal
Title: DIHYDROERGOTOXINE AND THE PASSIVE AVOIDANCE BEHAVIOUR IN
RATS WITH DAMAGED CHOLINERGIC INNERVATION OF THE NEOCORTEX
- Authors:
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Đorđević, Nebojša
Journal: Acta Facultatis Medicae Fluminensis
Number: 1
ISSN: 0065-1206
Volume: 16
Year: 1991
Pages: from 7 to 12
Number of references: 47
Language: hrvatski
Summary: The paper is dealing with the influence of
dihydroergotoxine onthe passive avoidance behaviour in rats with
bilateralelectrolytic lesions of the nucleus basalis. The mentioned
basalforebrain structure lesions result in deficit of the
cognitiveactivities. Dihydroergotoxine administered intraperitoneally
indoses of 4,9*10-9 mol*kg-1 or 4,9*10-8 mol*kg-1 improved passiveavoidance
in lesioned animals. In doses of 4,9*10-7 mol*kg-1 or4,9*10-6 mol*kg-1 did
not change and in dose of 2,45*10-5mol*kg-1 impaired the avoidance ability
in lesioned rats. Theconclusion is that the effect of dihydroergotoxine
isdose-dependent in this experimental model.
Keywords: nucleus basalis, dihydroergotoxine, learning, rats
- Type of paper
: Paper in journal
Title: THE EFFECT OF AMLODIPINE ON PASSIVE AVOIDANCE BEHAVIOUR IN
INTACT OR HYPOXIA-EXPOSED RATS
- Authors:
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Mršić, Jasenka (142272)
Journal: Acta Facultatis Medicae Fluminensis
Number: 1
ISSN: 0065-1206
Volume: 18
Year: 1993
Pages: from 25 to 31
Number of references: 23
Language: hrvatski
Summary: The influence of calcium channel blocker amlodipine, on
thelearning ability in intact and hypoxia-exposed rats has beenexamined.
The cognitive activities of the experimental animalswere examined in the
passive avoidance task during fourconsecutive days. Various doses (0,03 ,
0,1 , 0,3 or 1 mg/kg) ofamlodipine were injected on the first training
day,thirty minutesbefore the training session started. A part of animals
wasexposed to controlled hypoxic conditions immediately after thetraining
procedure. The avoidance time in relation to the totaltesting time was
measured. The results indicate that hypoxiastrongly impairs the learning
abilities in rats. Theadministration of amlodipine does not influence the
passiveavoidance behaviour in intact rats. On the contrary, all testeddoses
of amlodipine improved the learning ability in the ratswhose cognitive
deficiency was caused by hypoxia. It has alsobeen found that the effects of
the substance examined are dosedependent.
Keywords: cerebral hypoxia, learning, amlodipine, rats
- Type of paper
: Paper in journal
Title:
- Type of paper
: Paper in journal
Title: INFLUENCE OF NICARDIPINE ON THE BRAIN FREE FATTY ACIDS
LEVEL IN PENICILLIN-INDUCED SEIZURES IN RATS
- Authors:
- Eraković, Vesna
- Župan, Gordana (142160)
- Križ, Jasna
- Simonić, Ante (43202)
- Varljen, Jadranka (85515)
- Mršić, Jasenka (142272)
Journal: Acta Facultatis Medicae Fluminensis
ISSN: 0065-1206
Volume: 19
Year: 1994
Pages: from 19 to 22
Number of references: 14
Language: engleski
Summary: The aim of this study was to investigate the effect of the
calcium channel blocker, nicardipine, on the brain free fatty acids (FFAS)
level in chemically induced seizures in rats. The study was carried out on
Hannover-Wistar rats. Animals were anesthetized and placed in a stereotaxic
apparatus. Each of them received an injection of penicillin (5000 U/ml)
into the left lateral ventricle.Various doses of nicardipine (1; 3, 10 or
30 mg/kg i.p.)had been injected 30 minutes before the penicillin
application. The rats were decapitated five minutes after the occurrence of
epileptic seizures.FFAs were quantified by gas chromatography using the
internal standard method. The results demonstrate that: a) i.c.v. injection
of penicillin produced significant increase in the brain FFAs
concentration, and b) nicardipine, in the tested doses, did not prevent
with statistical significance the accumulation of the brain FFAs in the
rats with penicillin-induced seizures.
Keywords: epilepsy, brain, free fatty acids, nicardipine
- Type of paper
: Paper in journal
Title: EFFECTS OF INTAHIPPOCAMPAL INJECTION OF KAINIC ACID ON EEG
AND GROSS BEHAVIOR IN RATS
- Authors:
- Križ, Jasna
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Križ, Mladen
Journal: Acta Facultatis Medicae Fluminensis
ISSN: 0065-1206
Volume: 19
Year: 1994
Pages: from 13 to 18
Number of references: 14
Language: engleski
Summary: The purpose of this study was to record EEG activity and
the behavioral pattern changes after an intrahippocampal kainic acid
administration. The rats were anesthetized and placed in a stereotaxic
apparatus. Surface screw electrodes were placed over the frontal and
parietal cortex. Kainic acid was administered into the left dorsal
hippocampus. The EEG activity was recorded in a basal conditions and
5,10,15,30 and 60 minutes after the kainic acid application.During this
period complex behavioral pattern was also observed. EEG samples were
digitized and subjected to computerized frequency analysis. The kainic
acid-induced excitatory changes in EEG activity were followed by complex
behavioral syndrome that lasted for several hours. It has been concluded
that intrahippocampal injection of kainate may be useful experimental model
for investigations of partial seizures with complex simptomatology
(temporal lobe seizure disorders).
Keywords: kainic acid, epileptic seizures, EEG, frequency analysis, rat
- Type of paper
: Paper in journal
Title: EPILEPTIC SEIZURES INDUCE DAMAGE OF THE HIPPOCAMPAL
PYRAMIDAL NEURONS
- Authors:
- Križ, Jasna
- Župan, Gordana (142160)
- Mršić, Jasenka (142272)
- Tomac, Jelena
- Pernjak-Pugel, Ester
- Eraković, Vesna
- Simonić, Ante (43202)
- Batistić, Berislav
Journal: Acta Facultatis Medicae Fluminensis
ISSN: 0065-1206
Volume: 19
Year: 1994
Pages: from 23 to 25
Number of references: 2
Language: engleski
Summary: The purpose of this study was to investigate the influence
of the epileptic seizures on the structural integrity of the pyramidal
neurons of the CA1 subfield of the hippocampus. The epileptic seizures were
induced by the injections of benzylpenicillin or kainic acid into the left
cerebral ventricle. The administration of these substances produced
statistically significant decrease in the number of the pyramidal neurons
of the hippocampal CA1 region. Epileptic seizures induced by the penicillin
or kainic acid intracerebroventricular application caused also the
structural changes in the pyramidal cells in the CA1 subfield of the
hippocampus. Namely, a slight decrease in eosinophilic staining intensity
of the shrunken cytoplasma associated with darkly staining shrunken nucleus
was clearly observed in these neurons.
Keywords: epilepsy, hippocampus, rat
- Type of paper
: Paper in journal
Title: PIRACETAM AND OXIRACETAM PREVENT HYPOXIA-INDUCED
IMPAIRMENT OF PASSIVE AVOIDANCE BEHAVIOR IN RATS
- Authors:
- Župan, Gordana (142160)
- Mršić, Jasenka (142272)
- Simonić, Ante (43202)
Journal: Neurologia Croatica
Number: 4
ISSN: 0353-8842
Volume: 43
Year: 1994
Pages: from 233 to 240
Number of references: 22
Language: engleski
Summary: The influence of piracetam and oxiracetam on passive
avoidance behavior in intact and hypoxia-exposed rats was examined. Various
doses of the drugs tested were injected 60 minutes before the learning
trial. Some of the animals were exposed to controlled hypoxic conditions
immediately after the learning trial response had been acquired. Twenty
four hours later, the passive avoidance retention test was performed.
Hypoxia was found to cause a statistically significant impairment in the
retention of passive avoidance response. Piracetam and oxyracetam did not
influence passive avoidance behavior in intact animals. On the contrary,
all tested doses of the substances examined significantly improved the
learning ability in the rats in which cognitive deficiency was caused by
hypoxia. It was also found that the effects of the drugs tested were
dose-dependent.
Keywords: cerebral hypoxia, nootropics, passive avoidance behavior, rat
- Type of paper
: Paper in journal
Title: VULNERABILITY OF THE CA1 SUBFIELD OF THE HIPPOCAMPUS IN
HYPOXIC AND ISCHEMIC CONDITIONS IN RATS
- Authors:
- Župan, Gordana (142160)
- Mršić, Jasenka (142272)
- Simonić, Ante (43202)
- Pernjak-Pugel, Ester (133643)
- Tomac, Jelena (133676)
- Batistić, Berislav
Journal: Periodicum Biologorum
ISSN: 0031-5362
Volume: 96
Year: 1994
Pages: from 135 to 136
Number of references: 2
Language: engleski
Summary: The purpose of this study was to investigate the influence
of hypoxia/ischemia on the integrity of the hippocampal neurons of the CA1
region.The study was carried out on Hannover-Wistar rats weighing 250 g.
One group of animals was subjected to a period of oxygen deprivation
hypoxia.In the second group of animals the right common carotid artery was
occluded and a unilateral cerebral ischemia was produced. The animals of
the third group were subjected to the right common carotid artery
occlusion, allowed to recover for two hours and then exposed to the hypoxia
procedure. In the last group of the animals diffuse forebrain ischemia was
induced by four vessel occlusion procedure described ba Pulsinelli and
Brierley.Seven days after the described experimental procedures the brains
were perfused transcardially with 10% formalin, removed, postfixed,
dehydrated and embedded in paraffin. Coronal sections, 6 um thick, were
deparaffinated, stained with Hematoxylin and eosin and Cresyl violet. They
were examined by light miscroscope and the neuronal damage in the
hippacampus was evaluated by the neuronal density of the CA1 subfield, that
is the number of the CA1 neurons per 1 mm linear lenght of the stratum
pyramidale. It has been found that cerebral hypoxia did not produce any
significant neuronal damage in the hippocampal CA1 region. In contrast, a
statistically significant decrease in the number of the pyramidal cells
occured in the animals exposed to unilateral caritid occlusin or unilateral
carotid occlusion plus hypoxia or four vessel occlusion.In conclussion, our
results clearly show that the CA1 pyramidal cells in the hippocampus are
highly susceptible to cerebral ischemic insult.
Keywords: hypoxia, ischemia, hippocampus, rat
- Type of paper
: Paper in proceedings
Title: EFFECT OF NIMODIPINE ON THE BRAIN FREE ARACHIDONIC ACID
LEVEL IN HYPOXIA-EXPOSED RATS
- Authors:
- Župan, Gordana (142160)
- Eraković, Vesna
- Simonić, Ante (43202)
- Varljen, Jadranka (85515)
- Mršić, Jasenka (142272)
- Editors
- Jernej, Branimir
- Zdilar, Darko
- Bulat, Marin
- Kunec-Vajić, Estera
- Lacković, Zdravko
- Klarica, Marijan
Proceedings title: PHARMACOLOGICAL COMMUNICATIONS
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 139 to 141
Meeting: THE FIRST CROATIAN CONGRESS OF PHARMACOLOGY
Held: from 10/06/93 to 10/08/93
Summary: Accumulation of the free fatty acids (FFAs),
particularlyarachidonic acid,in the brain in ischemic conditions is a
resultof their liberation from membrane phospholipids. That process
ismediated by phospholipases which could be stimulated by increaseof
intracellular Ca 2+ concentration.The aim of this study was toinvestigate
the effect of nimodipine, calcium channel blocker, onthe brain free
arachidonic acid (FAA) level in hypoxia-exposedrats. The study was carried
out on Hannover-Wistar rats weighing250 g. The animals of the control group
were intact, drug naive.All other animals were subjected to a period of
oxygendeprivation hypoxia up to the lost of the righting reflex.Immediately
or 5 minutes, or 15 minutes or 60 minutes afterloosing the mentioned reflex
rats were decapitated, brains werequickly removed and frozen in the liquid
nitrogen. One group ofthe animals received 1 mg/kg nimodipine i.p. 30
minutes beforeexposure to hypoxia. Other animals were injected with
vehiclesolution (propylene glycol and ethanol, 50:50, V/V). The rats ofboth
mentioned groups were decapitated 15 minutes after loosingthe rigting
reflex. Lipid extracts prepared from frozen brainswere separated by
thin-layer chromatography. FFAs methyl esterswere prepared by methanolysis
and quanified by gaschromatography. Our results clearly demonstrated that
cerebralhypoxia induced progressive increase in the brain FAA
content.Maximal level of the FAA was detected 15 minutes after
cerebralhypoxia had been obtained. The decrease in the level of
mentionedfatty acid was observed 60 minutes after hypoxia
procedure.Administration of 1 mg/kg of nimodipine prevented theaccumulation
of the brain FFA caused by hypoxia. Namely, in thepresence of nimodipine
level of mentioned FFA in the brain ofhypoxia-exposed rats was
significantly lower in relation to thehypoxic drug naive or hypoxic
vehicle-treated animals. Thecontent of the FAA did not differ significantly
between theanimals of the control group and hypoxic nimodipine-treated
rats.In conclusion, our results demonstrated that nimodipine preventedthe
hypoxia-induced release of the brain FFA. It may beassumed that on the
basis of this mechanism,tested calciumchannel blocker may protect the
central nervous system againsthypoxia-induced damage.
Keywords: cerebral hypoxia, free fatty acids, arachidonic acid, nimodipine, brain, rat
- Type of paper
: Paper in proceedings
Title: REVERSAL OF ELECTROSHOCK-INDUCED AMNESIA BY AMLODIPINE IN
THE RAT
- Authors:
- Župan, Gordana (142160)
- Matešić, Damir
- Simonić, Ante (43202)
- Editors
- Jernej, Branimir
- Klarica, Marijan
- Zdilar, Darko
- Bulat, Marin
- Kunec-Vajić, Estera
- Lacković, Zdravko
Proceedings title: PHARMACOLOGICAL COMMUNICATIONS
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 142 to 144
Meeting: THE FIRST CROATIAN CONGRESS OF PHARMACOLOGY
Held: from 10/06/93 to 10/08/93
Summary: Dihydropyridine calcium channel blockers may affect
memoryprocesses. They can prevent the retrograde amnesia produced
bynitrogen hypoxia in one-trial passive avoidance learningexperiment. These
drugs can be also effective in diminishing therecent memory impairments
occuring in older nonhuman primates.Electroshock (ECS)-induced amnesia has
been proposed as atechnique for producing retrograde amnesia. The aim of
this studywas to examine the effects of amlodipine on ECS-induced amnesiain
the rat. The study was carried out on Hannover Wistar ratsweighing 150-200
g. Various doses (0.03; 0.1; 0.3; 1.0 mg/kg) ofamlodipine were given i.p.
The contol group received i.p.injection of vehicle. Thirty minutes later a
passive avoidancetask started according to the step-through procedure.In
thelearning trial each rat was placed in the illuminated compartmentof a
two-compartment apparatus, facing the guillotine door. Afterthe orientation
period (30 s), the door was raised. As soon asanimals placed all four paws
in the dark compartment theguillotine door was lowered and a 0.6 mA
scrambled shock wasdelivered through the grid flor for 1.0 s. The time
latencybefore entering the dark chamber was recorded. Immediately afterthe
learning trial, each animal was removed from the apparatusand received an
ECS (50 mA, 50 Hz, 0.4 s) through the ears. Onegroup of rats was subjected
to the same procedure but without ECS(sham ECS control group). Twenty four
hours later the retentiontrial was performed. It has been found that ECS
administrationimmediately after the learning trial strongly impaired
thestep-through latency of the retention trial in the rat. Ourresults also
demonstrated that pretreatment with amlodipinereverse the memory deficit in
ECS-treated rats. Statisticallysignificant enhancement of the retested
passive avoidancebehavior was produced by 0.3 and 1.0 mg/kg amlodipine.
Keywords: electroshock,amnesia, amlodipine, rat
- Type of paper
: Paper in proceedings
Title: THE INFLUENCE OF NIMODIPINE ON PENICILLIN-INDUCED
EPILEPTIC DISCHARGE IN RATS
- Authors:
- Župan, Gordana (142160)
- Križ, Jasna
- Simonić, Ante (43202)
- Editors
- Jernej, Branimir
- Klarica, Marijan
- Zdilar, Darko
- Bulat, Marin
- Kunec-Vajić, Estera
- Lacković, Zdravko
Proceedings title: PHARMACOLOGICAL COMMUNICATIONS
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 133 to 135
Meeting: THE FIRST CROATIAN CONGRESS OF PHARMACOLOGY
Held: from 10/06/93 to 10/08/93
Summary: influx of Ca 2+ into the neurons of the central nervous
system isthe first step in the generation of epileptic events. Inhibitionof
Ca 2+ may contribute to the therapeutic action of someanticonvulsants. Ca
2+ channel blockers have been reported tohave anticonvulsant activity in
certain experimental seizuresmodels and in some clinical settings. The aim
of the study was toinvestigate whether nimodipine has some anticonvulsant
activityin simple partial seizure activity caused by focal applicationof
penicillin. Experiments were performed on adult Wistar ratsweighing 200-250
g. The animals were divided into 5 groups,anesthetized with chloralhydrate
(300 mg/kg) and placed in astereotaxic apparatus. After a midline incision
four holes weredrilled and single electrodes were screwed into the skull
overthe frontal and parietal cortex of each hemisphere. The rats ofthe
control group 1 were drug naive. Other animals receivedinjection of saline
(2ul) (control group 2), or benzyl-penicillin(2000 U/2ul) into the left
motorcortex. In penicillin-treatedanimals the plastic cannula was placed
into the left lateralventricle and cemented to the skull. The rats of the
controlgroup 3 received intracerebroventricular (ICV) injection ofvehicle
solution. Other animals were ICV injected with nimodipine(100 ug/ul). After
described procedure had been done the animalswere placed in a grounded
metal box and connected via flexible wire to the electroencephalograph. The
EEG was recorded and grossbehaviour was observed. Administration of
penicillin into theleft motorcortex induced the spikes appearence
predominantely inthe region of the frontal cortex and ipsilateral parietal
cortex.Focal seizure activity appeared 3-5 min after drug administrationand
lasted for hours. After the seizure focus had beenestablished, it generated
the appearence of the epileptic spikesat irregular intervals (0.5-5.0 s).
They had amplitude of 0.5 toabout 2.0 mV and duration of 50-150 ms. ICV
administration ofnimodipine in penicillin-treated animals caused
supression offocal seizure activity. The first ICV injection of
nimodipinecaused a significant decrease in the frequency and in the
voltageof the cortical spikes. After repeated drug application
focalepileptic discharge was completely abolished.The results of
ourexperiment clearly demonstrate that nimodipine possesses
certainanticonvulsant action in penicillin-induced focal seizuresactivity
in rats.
Keywords: penicillin, epileptic discharge, nimodipine, rat
- Type of paper
: Summary in proceedings
Title: LESIONS OF THE NUCLEUS BASALIS IN NEWBORN, ADULT AND AGING
RATS: EFFECT ON CORTICAL ChAT ACTIVITY AND BEHAVIOUR
- Authors:
- Casamenti, Fiorella
- Scali, Carla
- Župan, Gordana (142160)
- Pepeu, Giancarlo
- Editors
- Marešova, D.
- Tuček, s
- Štastny, F.
Proceedings title: ABSTRACTS OF THE IUPS REGIONAL MEETING IN PRAGUE 1991
Language: engleski
Place: Prag, Češka
Year: 1991
Pages: from 54 to 0
Meeting: IUPS REGIONAL MEETING
Held: from 06/30/91 to 07/05/91
Summary: Experiments aimed to investigate the effects of nucleus
basalis(NB) lesions on cortical cholinergic system and
behaviour,spontaneous recovery and recovery induced by GM1 ganglioside
werecarried out in rats of different age. Quisqualic acid unilaterallesions
were placed in the NB of 14;17 and 21-day old rats. A 50%decrease in
choline acetyltransferase (ChAT) in the ipsilateralcortex was found 8 days
after lesioning. A passive avoidance"step-through" response was acquired by
the sham operated 21-oldrats only. The 21-day old lesioned rats, sham
operated andlesioned younger rats were unable to negotiate the test.
Recovery3 months after lesioning will be investigated. In 3-months oldrats,
with ibotenic acid unilateral or bilateral NB lesions, GM1ganglioside, 30
mg/kg i.p. for 20 days, beginning immediatelyafter lesioning, reduces ChAT
decrease and behavioral impairment.A 2 days treatment was ineffective. In
18-month old rats aunilateral ibotenic acid lesion was followed by
behavioralimpairment and an unexpected bilateral decrease in ChAT
activity.GM1 (30 and 60 mg i.p. for 20 days) reduced the contralateralChAT
decrease and behavioral impairment.
Keywords: lesions of the nucleus basalis, cortical choline acetyltransferase activity, behaviour, rats of different age
- Type of paper
: Summary in proceedings
Title: THE EFFECTS OF NICARDIPINE AND NITRENDIPINE ON
HYPOXIA-INDUCED MEMORY IMPAIRMENT IN THE RAT
- Authors:
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Mršić, Jasenka (142272)
- Editors
- Scatton, B.
Proceedings title: EUROPEAN NEUROPSYCHOPHARMACOLOGY vol. 1/3
Language: engleski
Place: Amsterdam, Nizozemska
Year: 1991
ISBN/ISSN: 0924-977X
Pages: from 407 to 0
Meeting: IVth CONGRESS OF THE EUROPEAN COLLEGE OF NEUROPSYCHOPHARMACOLOGY
Held: from 10/06/91 to 10/09/91
Summary: Hypoxic damage of cells is associated with a precipitous
influxof calcium from the extracellular to the intracellularcompartment,
and as a consequence, intracellular calciumconcentration is increasing. The
elevated cytosolic calciumconcentration induces different vascular and
biochemicaldisturbances like reduction in cerebral blood flow, impairment
ofthe mitochondrial function with concomitant energy failure etc.The
purpose of this study was to examine the effects ofnicardipine and
nitrendipine on learning ability in hypoxiaexposed rats. The study was
carried out on Hannover Wistar ratsweighing 250 g. Various doses (0.03;
0.1; 0.3; 1.0 mg/kg) ofnicardipine and nitrendipine were given i.p. Thirty
minutes laterpassive avoidance task was performed according to
thestep-through procedure, modified by Jarvik and Kopp (1967).Immediately
after the training animals were subjected to periodof oxygen deprivation
hypoxia up to the lost of righting reflex.It has been found that both
nicardipine and nitrendipine wereeffective in reversing the memory deficits
in hypoxia exposedrats. All tested doses of nitrendipine and 0.3 or 1.0
mg/kg ofnicardipine led to a significant enhancement of the
passiveavoidance in hypoxia treated rats. Nicardipine at the doses of0.03
or 0.1 mg/kg slightly, but not significantly improved theperformance of
behavioral task.
Keywords: cerebral hypoxia, nicardipine, nitrendipine, learning, rat
- Type of paper
: Summary in proceedings
Title: THE EFFECTS OF CALCIUM CHANNEL BLOCKERS NIMODIPINE AND
NICARDIPINE AND NOOTROPIC DRUGS PIRACETAM AND OXIRACETAM ON HYPOXIA-
INDUCED MEMORY IMPAIRMENT IN THE RAT
- Authors:
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Mršić, Jasenka (142272)
Proceedings title: NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY, suppl. vol. 346
Language: engleski
Place: Berlin, Njemačka
Year: 1992
ISBN/ISSN: 0028-1298
Pages: from 23 to 0
Meeting: AUTUMN MEETING DEUTSCHE GESELLSCHAFT FUR PHARMAKOLOGIE UND TOXICOLOGIE
Held: from 09/06/92 to 09/09/92
Summary: Cerebral hypoxia is associated with a various
functionaldisturbances and damage of neurons. We investigated the effectsof
calcium channel blockers (nimodipine, nicardipine) andmetabolic enhancers
(piracetam, oxiracetam) on hypoxia inducedcognitive deficit in the rat. The
study was carried out onHannover Wistar rats weighing 250 g. Variuos doses
of nimodipineand nicardipine (0.03; 0.1; 0.3; 1.0 mg/kg) were given
i.p.Thirty or sixty minutes later passive avoidance task wasperformed
according to the step-through procedure modified byJarvik and Kopp (1967).
Immediately after the training, animalswere subjected to a period of oxygen
deprivation hypoxia up tothe lost of righting reflex. Twenty four hours
later passiveavoidance retention was tested by using mentioned
step-throughprocedure. It has been found that all tested drugs were
effectivein reversing the memory deficits in hypoxia exposed rats.
Alltested doses of mentioned substances led to significantenhancement of
the passive avoidance in hypoxia treated rats.
Keywords: cerebral hypoxia, calcium channel blockers, nootropics, memory, rat
- Type of paper
: Summary in proceedings
Title: NIMODIPINE PREVENTS ELECTROSHOCK-INDUCED AMNESIA IN THE
RAT
- Authors:
- Župan, Gordana (142160)
- Matešić, Damir
- Simonić, Ante (43202)
- Vitezić, Dinko
Proceedings title: NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY, suppl.vol. 346
Language: engleski
Place: Berlin, Njemačka
Year: 1992
ISBN/ISSN: 0028-1298
Pages: from 23 to 0
Meeting: AUTUMN MEETING DEUTSCHE GESELLSCHAFT FUR PHARMAKOLOGIE UND TOXICOLOGIE
Held: from 09/06/92 to 09/09/92
Summary: Dihydropyridine calcium channel blocker, nimodipine, may
affectmemory processes. Therefore, the purpose of the present study wasto
examine the effects of nimodipine on electroshock (ECS)induced amnesia. The
study was carried out on Hannover Wistarrats weighing 150-200 g. Nimodipine
(0.03; 0.1; 0.3; 1.0 mg/kg)was given i.p. Thirty minutes later passive
avoidance task wasperformed according to the step-through procedure.
Namely, therats were placed individually into the light compartment of
atwo-compartment apparatus. When they walked into the darkcompartment they
received a foot shock.Immediately after thetraining trial the animals
received an ECS through the ears. Onegroup of rats was subjected to the
same procedure but without ECS(sham ECS treated control group). Twenty-four
hours later theretest step-through latency was measured. It has been found
thatECS administered to vehicle treated animals immediately after
thetrainig trial caused statistically significant decrease of theretest
latency as compared to sham ECS treated control animals.Our experiments
also demonstrated that pretreatment withnimodipine prevented in a
dose-dependent manner the disruption ofthe avoidance response caused by ECS
application. Statisticallysignificant effects were produced by 0.1; 0.3 and
1.0 mg/kgnimodipine. These results support the hypothesis thatperturbations
in calcium homeostasis may contribute to the memorydeficits associated with
ECS application.
Keywords: elctroshock, amnesia, nimodipine, rat
- Type of paper
: Summary in proceedings
Title: INFLUENCE OF OXIRACETAM ON PASSIVE AVOIDANCE BEHAVIOR IN
SCOPOLAMINE TREATED RATS
- Authors:
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Vlahović, Vera
Proceedings title: NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY suppl.vol.346
Language: engleski
Place: Berlin, Njemačka
Year: 1992
ISBN/ISSN: 0028-1298
Pages: from 23 to 0
Meeting: AUTUMN MEETING DEUTSCHE GESELLSCHAFT FUR PHARMAKOLOGIE UND TOXICOLOGIE
Held: from 09/06/92 to 09/09/92
Summary: Amnesia induced by anticholinergic agent, scopolamine, has
beenproposed as a short-term amnesia model representative ofdementia.
Oxiracetam is able to facilitate learning and memoryprocesseses. The
possibility that oxiracetam acts on braincholinergic mechanisms is
supported by some findings. Therefore,the aim of our study was to
investigate the effects ofscopolamine on learning ability and whether
oxiracetam couldprevent scopolamine-induced impairment of memory in the
rat. Theexperiments were carried out on Wistar rats weighing 150-200
g.Passive avoidance behaviour was studied in a step-through type ofpassive
avoidance situation. A two compartment apparatus with agrid floor which
could be electrified was used. During thelearning trial the rat was placed
in the illuminated compartment.After 30 sec. the guillotine door was opened
and the latencybetween the door opening and the entrance into the
darkcompartment was measured. When the rat walked into the dark boxit
received a foot shock. The retention trial was carried out 24hrs later.
Scopolamine (0.1; 0.3; 0.5 or 1 mg/kg) was injectedi.p. 30 min. before the
learning trial. Oxiracetam (50; 100; 300mg/kg) was administered i.p. 30
min. prior to scopolamine (0.3mg/kg).Our experiments have demonstrated that
all tested doses ofscopolamine significantly decreased retention of
passiveavoidance conditioned response. It has also been found
thatoxiracetam at the dose of 50 mg/kg completely
preventedscopolamine-induced amnestic effect in the rat. In conclusion,our
experiments indirectly confirm the hypothesis that oxiracetaminfluences on
central cholinergic mechanisms.
Keywords: amnesia, scopolamine, oxiracetam, rat
- Type of paper
: Summary in proceedings
Title: EFFECT OF CALCIUM CHANNEL BLOCKERS NITRENDIPINE AND
FELODIPINE ON ELECTROSHOCK-INDUCED AMNESIA IN THE RAT
- Authors:
- Župan, Gordana (142160)
- Matešić, Damir
- Simonić, Ante (43202)
Proceedings title: NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY, suppl.vol. 347
Language: engleski
Place: Berlin, Njemačka
Year: 1993
ISBN/ISSN: 0028-1298
Pages: from 137 to 0
Meeting: 34th SPRING MEETING DEUTSCHE GESELLSCHAFT FUR PHARMAKOLOGIE UND TOXIKOLOGIE
Held: from 03/16/93 to 03/18/93
Summary: Electroshock-induced amnesia has been proposed as a
technique forproducing retrograde amnesia. The aim of our study was
toinvestigate whether calcium channel blockers, nitrendipine andfelodipine,
can prevent memory deficit caused by electroshock(ECS) application.The
study was carried out on Hannover Wistarrats weighing 150-200 g. Various
doses of nitrendipine orfelodipine (0.03; 0.1; 0.3; 1.0 mg/kg) were given
i.p.Thirtyminutes later the passive avoidance task was performed
accordingto the step-through procedure. A two-compartment apparatus with
agrid floor which could be electrified was used. During thelearning trial
the rat was placed in the light compartment. After30 seconds the guillotine
door was opened and the latency betweenthe door opening and the entrance
into the dark compartment wasmeasured. When the rat walked into the dark
part of the apparatusit received a foot shock. Immediately after the
training trialthe animal received an ECS through the ears. The retention
trialwas carried out 24 hours later. It has been found that bothtested
drugs were effective in reversing the memory deficits inECS-treated rats.
Statistically significant enhancement of thepassive avoidance behavior was
produced by 0.1; 0.3 and 1.0 mg/kgof nitrendipine and 1.0 mg/kg of
felodipine.
Keywords: electroshock, amnesia, nitrendipine, felodipine, rat
- Type of paper
: Summary in proceedings
Title: REVERSAL OF HYPOXIA-INDUCED AMNESIA BY ARECOLINE IN THE
RAT
- Authors:
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Mršić, Jasenka (142272)
Proceedings title: NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY suppl. vol. 347
Language: engleski
Place: Berlin, Njemačka
Year: 1993
ISBN/ISSN: 0028-1298
Pages: from 137 to 0
Meeting: 34th SPRING MEETING DEUTSCHE GESELLSCHAFT FUR PHARMAKOLOGIE UND TOXIKOLOGIE
Held: from 03/16/93 to 03/18/93
Summary: Numerous evidences suggest that cholinergic
neurotransmissionactivity of central nervous system is strongly involved in
memoryprocesses and that it is particullary vulnerabile to
hypoxia.Therefore, the present study was designed to examine
ifhypoxia-induced amnesia could be reversed by cholinergic
agonist,arecoline. The study was carried out on Hannover Wistar
ratsweighing 250 g. Arecoline (0.1; 0.3; 1.0 mg/kg) was given i.p.The
control group received saline i.p. Ten minutes later passiveavoidance
behavior was studied according to the step-throughprocedure. A two
compartment apparatus with a grid floor whichcould be electrified was used.
During the learning trial the ratwas placed in the illuminated compartment.
Ten seconds later theguillotine door was raised and the latency between the
dooropening and the entrance into the dark compartment wasmeasured.After
entering into the dark part of the apparatus, theanimals received an
unavoidable foot shock. Immediatelly afterthe training, the rats were
subjected to a period of oxygendeprivation hypoxia. The retention trial was
carried out 24 hourslater. Our results demonstrate that hypoxia produced
significantimpairment of passive avoidance behavior. Arecoline was
effectivein reversing the memory deficits in hypoxia exposed rats.
Namely,all tested doses of mentioned drug produced significantimprovement
of the passive avoidance task in hypoxic animals.These results support the
hypothesis that impairment ofcholinergic neurotransmission activity is
involved in memorydeficit in hypoxic rats.
Keywords: cerebral hypoxia, memory, arecoline, rat
- Type of paper
: Summary in proceedings
Title: REVERSAL OF PASSIVE AVOIDANCE BEHAVIOR SIX MONTHS AFTER
DISRUPTION OF CORTICAL CHOLINERGIC INNERVATION
- Authors:
- Župan, Gordana (142160)
- Simonić, Ante (43202)
Proceedings title: NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY suppl. vol. 347
Language: engleski
Place: Berlin, Njemačka
Year: 1993
ISBN/ISSN: 0028-1298
Pages: from 137 to 0
Meeting: 34th SPRING MEETING DEUTSCHE GESELLSCHAFT FUR PHARMAKOLOGIE UND TOXIKOLOGIE
Held: from 03/16/93 to 03/18/93
Summary: The ventromedial globus pallidus and adjacent
magnocellularneurones of basal forebrain (NB) provide the major
cholinergicinnervation to the neocortex in the rat. The purpose of
thisstudy was to examine the passive avoidance behavior twenty daysand six
months after the NB lesion. Male Wistar rats weighing200-250 g were used.
Unilateral or bilateral electrolytic NBlesions were made stereotaxically.
The site of the lesions washistologically verified. Twenty days later the
passive avoidancetask according to the procedure of Ashford and Jones
(1976) wasperformed. A chamber with a grid floor continously electrifiedwas
used. The central area of the platform contained a woodenplatform. Each rat
was placed on the platform and left in theapparatus for three minutes. The
total time spent on the platformwas recorded. A total of four consecutive
daily sessions weregiven. Six months later the passive avoidance task was
repeatedin bilateral lesioned rats. Results of our experiments show
thatboth unilateral and bilateral lesions of the NB impaired thepassive
avoidance responses in the rat. The effect of bilaterallesions was
statistically significant.Six months latersignificant improvement of
passive avoidance behavior was notedin bilateral lesioned rats. It is
concluded that destruction ofthe NB neurons can be compensated by the
plasticity of theresidual NB neurons and other central cholinergic
andnoncholinergic structures which are involved in learning andmemory
processes.
Keywords: nucleus basalis, unilateral lesion, bilateral lesions, plasticity of the central nervous system, rat
- Type of paper
: Summary in proceedings
Title: EFFECTS OF HYPOXIA ON FREE FATTY ACID POOL IN THE BRAIN
- Authors:
- Varljen, Jadranka (85515)
- Eraković, Vesna
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Milin, Čeda (70123)
- Mršić, Jasenka (142272)
Proceedings title: HB 93
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 141
Meeting: ANNUAL MEETING OF CROATIAN BIOCHEMISTS WITH INTERNATIONAL PARTICIPATION
Held: from 06/17/93 to 06/18/93
Summary: Cerebral hypoxia activates membrane phospholipases with
formationof free fatty acids (FFA), in particular arachidonic acid. TheFFA
are assumed to have detrimental effect on mitochondrial andplasma membrane
functions. The present experiments were designedto study FFA levels in the
brain during various time intervalsafter hypoxic brain injury. The study
was carried out on HannoverWistar rats weighing 250 g. The animals were
subjected to 3.5 V%hypoxia, until loosing of righting reflex. Immediately
or 5 or15 or 60 minutes after hypoxia animals were decapited, brainswere
removed quickly and frozen in the liquid nitrogen. Lipidextracts were
prepared from frozen brains fractioned by TLC andfatty acid methyl esters
were prepared by methanolysis andquantified by GLC using internal standard
method. In nonhypoxicconditions brain FFA pool consists mainly of palmitic
acid,stearic acid, oleic acid with smaller amount of arachidonic acid.Our
results suggest that hypoxia induces progressive increase intotal FFA
content and that maximum amount of free arachidonicacid is detected 15
minutes after cerebral hypoxia.
Keywords: hypoxia, brain, free fatty acids, arachidonic acid, rat
- Type of paper
: Summary in proceedings
Title: EXPERIMENTAL MODELS OF EPILEPSY
- Authors:
- Križ, Jasna
- Mršić, Jasenka (142272)
- Simonić, Ante (43202)
- Župan, Gordana (142160)
- Križ, Mladen
- Paučić-Kirinčić, Ela
- Editors
- Peršić, Mladen
Proceedings title: PAEDIATRIA CROATICA suppl. 1, vol. 37
Language: hrvatski
Place: Zagreb
Year: 1993
ISBN/ISSN: 1330-1403
Pages: from 72 to 0
Meeting: I. KONGRES HRVATSKOGA PEDIJATRIJSKOG DRUŠTVA
Held: from 09/29/93 to 10/02/93
Summary: Experimental models of epilepsy have very important role in
ourunderstanding of underlying mechanisms of seizure activity. Theaim of
our experiments was to study the penicillin inducedepileptic seizures in
rats. The exact mechanisms underlying therat model of epilepsy induced by
penicillin are still unclear butit is suppsed that penicillin caused the
preservation ofCl-mediated hyperpolarizing potentials caused by the
activationof GABAa receptors.The experiments were carried out on Wistarrats
weighing 250-350 g. All the animals were anesthetized andplaced in the
stereotaxic apparatus. Focal application ofpenicillin into the left motoric
cortex produced focal epileptic discharge. Generalized seizures appeared
after penicillinadministration into the cerebral ventricle or
basolateralamygdaloid nucleus. The EEG was recorded and gross behavior
wasobserved.
Keywords: penicillin, focal epilepsy, generalized epilepsy, rat
- Type of paper
: Summary in proceedings
Title: EXPERIMENTAL MODELS OF CEREBRAL HYPOXIA AND ISCHEMIA
- Authors:
- Župan, Gordana (142160)
- Simonić, Ante (43202)
- Križ, Jasna
- Mršić, Jasenka (142272)
- Križ, Mladen
- Editors
- Peršić, Mladen
Proceedings title: PAEDIATRIA CROATICA suppl. 1, vol.37
Language: hrvatski
Place: Zagreb
Year: 1993
ISBN/ISSN: 1330-1403
Pages: from 72 to 0
Meeting: I. KONGRES HRVATSKOGA PEDIJATRIJSKOG DRUŠTVA
Held: from 09/29/93 to 10/02/93
Summary: Cerebral hypoxia/ischemia are characterized with
variousbiochemical and functional disturbances. They are presented
innumerous clinical syndromes and diseases (perinatalencephalopathy, head
trauma etc.). The aim of our study was tostandardize models of
hypoxia/ischemia in order to investigatecerebroprotective effects of
numerous drugs. The experiments werecarried our on Hannover Wistar rats
weighing 200-250 g. In themodel of cerebral hypoxia the animals were placed
in the airtight plexiglas box (790 ml) through which a mixture of
purenitrogen and atmospheric air circulated. The oxygenconcentration was
gradually reduced by the animal breathinguntill the level of 3.5% of oxygen
was reached. The percentageof oxygen was continiously measured and
controled by an oxygenmeasuring device untill the loss of righting reflex
was reached.Global ischemia was induced by four vessel occlusion. Namely,
aday after bilateral electrocoagulation of vertebral arteries hadbeen done
both carotid arteries were ligated on the level of C1during 20 minutes. In
that period on EEG isoelectric line wasrecorded. Tested compounds were
administered before the formerprocedure was done in order to investigate
their cerebroprotective effects.
Keywords: cerbral hypoxia/ischemia, experimental procedures, rat
- Type of paper
: Summary in proceedings
Title:
- Type of paper
: Summary in proceedings
Title: ANTIAMNESTIC EFFECT OF TETRAHYDROAMINOACRIDINE IN
HYPOXIA-EXPOSED RATS
- Authors:
- Mršić, Jasenka (142272)
- Župan, Gordana (142160)
- Simonić, Ante (43202)
Proceedings title: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, suppl.vol. 349
Language: engleski
Place: Berlin, Njemačka
Year: 1994
ISBN/ISSN: 0028-1298
Pages: from R101
Meeting: 35th SPRING METTING DEUTSCHE GESELLSCHAFT FUR EXPERIMENTELLE UND KLINISCHE PHARMAKOLOGIE UND TOXICOLOGIE
Held: from 03/15/94 to 03/17/94
Summary: The study was designed to determine the efficacy of
acetylcholinesterase inhibitor tetrahydroaminoacridine (THA) in preventing
the memory decline in hypoxia-exposed animals. The study was carried out on
Hannover-Wistar rats weighing 250 g. Various doses of THA (0.1; 0.3; 1.0
mg/kg) were given i.p. Sixty minutes later passive avoidance task was
performed according to the step-through procedure. Immediately after the
training trial animals were subjected to a period of oxygen deprivation
hypoxia. The level of 3.5V% of oxygen was reached in about 20 minutes and
maintained up to the loss of righting reflex. The retention trial was
carried out 24 hours later. It has been found that all tested doses of
mentioned cholinergic agent led to a significant enhancement of the passive
avoidance in hypoxia treated rats. Our results confirm the hypothesis that
increase of central cholinergic activity can antagonize memory decline in
hypoxia-exposed rats.
Keywords: cerebral hypoxia, amnesia, tetrahydroaminoacridine, rat
- Type of paper
: Summary in proceedings
Title: DYNAMICS OF BRAIN FREE FATTY ACID POOL IN
PENICILLIN-INDUCED EPILEPTIC SEIZURES IN RATS
- Authors:
- Eraković, Vesna
- Križ, Jasna
- Mršić, Jasenka (142272)
- Župan, Gordana (142160)
- Varljen, Jadranka (85515)
- Milin, Čeda (70123)
- Simonić, Ante (43202)
Proceedings title: ABSTRACT BOOK
Language: engleski
Place: Helsinki, Finska
Year: 1994
Pages: from 150
Meeting: FEBS SPECIAL MEETING BIOLOGICAL MEMBRANES
Held: from 06/26/94 to 07/01/94
Summary: The aim of this study was to determine free fatty acids
(FFAs) level in the rat brain during various time intrervals after the
chemically induced seizures. The study was carried out on Hannover-Wistar
rats. The animals of the control group were intact. Other animals were
anesthetized and placed in stereotaxic apparatus. They received penicillin
injection (5000 u/5ul) or vehiculum into the left lateral ventricle and
were decapitated immediately or 5 or 15 min. after appearance of epileptic
seizures. The brains were removed and frozen in the liquid nitrogen. The
frozen brains were weighed and homogenized in chloroform-methanol (2:1,
V/V) for lipid extraction. The FFAs were separated by thin-layer
chromatography,methyl esters were prepared by methanolysis and quantified
by gas chromatography using internal standard method. The
penicillin-induced seizures produced an increase in total brain FFAs with
the largest relative increase in free arachidonic acid level reaching
plateau within min after the appearance of the epileptic seizures these
results attribute to the theory that calcium influx into the cell and
phospholipases activation could be one of the pathophysiological events
during epileptic brain damage
Keywords: penicillin-induced epilepsy, brain free fatty acids, dynamics, rat
- Type of paper
: Summary in proceedings
Title: NIMODIPINE PREVENTS THE RISE IN THE BRAIN FREE ARACHIDONIC
ACID LEVEL IN HYPOXIA-EXPOSED RATS
- Authors:
- Mršić, Jasenka (142272)
- Župan, Gordana (142160)
- Eraković, Vesna (900727)
- Varljen, Jadranka (85515)
- Simonić, Ante (43202)
- Križ, Jasna
Proceedings title: CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, suppl.1, vol.72
Language: engleski
Place: Ottawa, Kanada
Year: 1994
ISBN/ISSN: 0008-4212
Pages: from 437
Meeting: XIIth INTERNATIONAL CONGRESS OF PHARMACOLOGY
Held: from 07/24/94 to 07/29/94
Summary: The study was undertaken to investigate the effects of
nimodipine on the brain free arachidonic acid (FAA) level in
hypoxia-exposed rats. The study was carried out on Hannover-Wistar rats.
The animals of the control group were intact, drug naive. All other animals
were subjected to a period of oxygen deprivation hypoxia until the lost of
righting reflex. Immediately or 5 or 15 or 6o min. after loosing the
mentioned reflex rats were decapiteted and brains were frozen in liquid
nitrogen . One group of the animals was injected with vehicle solution
while other animals were pretreated with various doses of nimodipine, i.p.
30 min before hypoxia exposure. The rats of mentioned groups were
decapitated 15 min. after loosing the righting reflex. The free fatty acids
were separated by thin-layer chromatography, methyl esters were prepared by
menthanolysis and quanitified by gas chromatography. The level of FAA was
measured. Our results demonstrate that cerebral hypoxia induced progressive
increase in the brain FAA content. Maximal level was detected 15 min. after
cerebral hypoxia had been obtained. Administration of various doses of
nimodipine prevented the accumulation of the brain FAA caused by hypoxia.
Statistically significant protection was obtained with 0.1; 0.3 and 1.0
mg/kg of nimodipine.
Keywords: cerebral hypoxia, arachidonic acid, brain, nimodipine, rat
- Type of paper
: Summary in proceedings
Title: THE INFLUENCE OF NIMODIPINE AND AMLODIPINE ON THE BRAIN
FREE ARACHIDONIC ACID LEVEL AND BEHAVIOR IN HYPOXIA-EXPOSED RATS
- Authors:
- Župan, Gordana (142160)
- Mršić, Jasenka (142272)
- Eraković, Vesna (900727)
- Simonić, Ante (43202)
- Varljen, Jadranka (85515)
Proceedings title: CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, suppl. 1, vol.72
Language: engleski
Place: Ottawa, Kanada
Year: 1994
ISBN/ISSN: 0008-4212
Pages: from 407
Meeting: XIIth INTERNATIONAL CONGRESS OF PHARMACOLOGY
Held: from 07/24/94 to 07/29/94
Summary: The purpose of this study was to investigate the effects of
nimodipine and amlodipine on the brain free arachidonic acid (FAA) level
and learning ability in hypoxia-exposed rats. The experimental animals were
injected with 0.3 or 1.0 mg/kg of tested drugs. Thirty min. later the
learning ability was tested in a passive avoidance task according to the
step-through procedure. immediately after the learning trial,the animals
were subjected to a period of oxygen deprivation hypoxia until the lost the
righting reflex.The retention trial was carried out 24 hours later. A part
of animals used for biochemical investigations was pretreated with calcium
channel blockers and exposed to the hypoxic conditions. Fifteen min. after
loosing the righting reflex they were decapitated and brains were frozen in
liquid nitrogen.The level of the brain FAA was quantified by gas
chromatography. It has been found that nimodipine was effective in
reversing the significant increase of the brain FAA level and memory
decline in hypoxia-exposed rats. Amlodipine did not influence the content
of the brain FAA, but produced significant improvement of the passive
avoidance behavior in hypoxia conditions.
Keywords: hypoxia, nimodipine, amlodipine, behavior, brain, arachidonic acid, rat
- Type of paper
: Summary in proceedings
Title: EFFECT OF NIMODIPINE ON THE BRAIN FREE FATTY ACID LEVEL IN
RATS WITH PENICILLIN-INDUCED SEIZURES
- Authors:
- Križ, Jasna
- Župan, Gordana (142160)
- Varljen, Jadranka (85515)
- Eraković, Vesna
- Simonić, Ante (43202)
- Mršić, Jasenka (142272)
Proceedings title: CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, suppl.1, vol.72
Language: engleski
Place: Ottawa, Kanada
Year: 1994
ISBN/ISSN: 0008-4212
Pages: from 398
Meeting: XIIth INTERNATIONAL CONGRESS OF PHARMACOLOGY
Held: from 07/24/94 to 07/29/94
Summary: The aim of this study was to investigate the influence of
nimodipine on the brain free fatty acids (FFAs) level in penicillin-induced
seizures in rats. The animals received penicillin (5000 u/5ul) into the
left lateral ventricle. One group of rats were injected with vehicle, while
others received 1.0 or 30 mg/kg of nimodipine i.p. 30 min. prior to
penicillin application.Five min. after appearance of epileptic seizures the
rats were decapitated.The FFAs were separated by thin-layer chromatography,
methyl esters were preapared by methanolysis and quantified by gas
chromatography. Our results demonstrate that penicillin-induced seizures
produced an increase in brain FFAs level. Administration of 30 mg/kg of
nimodipine prevented the accumulation of the brain FFAs manner, while the
dose of 1.0 mg/kg was ineffective.
Keywords: penicillin-induced seizures, nimodipine, free fatty acids, brain, rat
- Type of paper
: Summary in proceedings
Title: CHANGES IN BRAIN FREE FATTY ACID LEVEL INDUCED BY HYPOXIA
AND EPILEPSY IN RATS
- Authors:
- Eraković, Vesna
- Varljen, Jadranka (85515)
- Mršić, Jasenka (142272)
- Križ, Jasna
- Župan, Gordana (142160)
- Simonić, Ante (43202)
Proceedings title: CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, suppl.1, vol.72
Language: engleski
Place: Ottawa, Kanada
Year: 1994
ISBN/ISSN: 0008-4212
Pages: from 608
Meeting: XIIth INTERNATIONAL CONGRESS OF PHARMACOLOGY
Held: from 07/24/94 to 07/29/94
Summary: The aim of our study was to determine extent of brain cell
damage in experimental model of controlled hypoxia and chemically induced
seizures in rats. The animals of the control group were intact. Four groups
of rats were subjected to a period of oxygen deprivation hypoxia.
Immediately or 5 or 15 or 60 minutes after loosing the righting reflex rats
were decapitated. Other two groups of animals were anesthetized, placed in
a stereotaxic apparatus and injected with penicillin (5000 u/5ul) i.c.v.
They were decapitated 5 or 15 min. after appearance of epileptic seizures.
In all hypoxia or penicillin treated rats the brains were removed and
frozen in the liquid nitrogen. The frozen brains were weighed and
homogenized in chloroform-methanol (2:1, V/V) for lipid extraction. The
FFAs were separated by thin-layer chromatography, methyl esters were
prepared by methanolysis and quantified by gas chromatography. Our results
demonstrated that cerebral hypoxia induced progressive increase in the
brain free arachidonic acid level with maximum detected 15 min. after
cerebral hypoxia had been obtained. The penicillin-induced epilepsy
produced an increase in brain free arachidonic acid as well as total FFAs
level reaching the platou in 5 min. The increase in free arachidonic acid
content was significantly higher in experimental model of chemically
induced seizures then in controlled hypoxia.
Keywords: cerebral hypoxia, epilepsy, free fatty acids, brain, rat
- Type of paper
: Summary in proceedings
Title: DYNAMICS OF INDIVIDUAL BRAIN FREE FATTY ACIDS UNDER
HYPOXIC CONDITIONS IN RATS
- Authors:
- Eraković, Vesna (900727)
- Mršić, Jasenka (142272)
- Križ, Jasna
- Župan, Gordana (142160)
- Varljen, Jadranka (85515)
- Simonić, Ante (43202)
Proceedings title: ABSTRACTS
Language: engleski
Place: Berlin, Njemačka
Year: 1994
Pages: from 36
Meeting: 3rd INTERNATIONAL SYMPOSIUM ON HYPOXIA
Held: from 09/22/94 to 09/24/94
Summary: The present study was undertaken to examine dynamics of
brain free palmitic, stearic, oleic and arachidonic acid during various
time intervals after hypoxic brain injury. The animals were subjected to
3.5 V% hypoxia until loosing of righting reflex. Immediately or 5 or 15 or
60 min. after hypoxia animals were decapitated, brains were removed quickly
and frozen in the liquid nitrogen. Lipids were extracted from frozen brains
and fractioned by TLC. Fatty acid methyl esters were prepared by
methanolysis and quantified by GLC using internal standard method.Our
results clearly demonstrated that cerebral hypoxia induced progressive
increase in the brain free fatty acids content. Palmitic, stearic and oleic
acids show same liberation pattern, reaching statistically significant
level 60 min. after the loosing of righting reflex. Maximal level of the
free arachidonic acid was detected 15 min. after cerebral hypoxia had been
obtained. Sixty min. after hypoxia procedure the level of mentioned free
fatty acid decreased to the control value. It could be concluded that
cerebral hypoxia induced different liberation modes of individual free
fatty acids.
Keywords: hypoxia, brain, individual free fatty acids, dynamics, rat
- Type of paper
: Summary in proceedings
Title: IFENPRODIL ANTAGONIZES AMNESTIC EFFECT OF HYPOXIA IN RATS
- Authors:
- Mršić, Jasenka (142272)
- Župan, Gordana (142160)
- Simonić, Ante (43202)
Proceedings title: ABSTRACTS
Language: engleski
Place: Berlin, Njemačka
Year: 1994
Pages: from 66
Meeting: 3rd INTERNATIONAL SYMPOSIUM ON HYPOXIA
Held: from 09/22/94 to 09/24/94
Summary: This study was designed to examine the influence of variuos
doses of the NMDA channel blocker, ifenprodil, on the retention of
passive avoidance in normoxic and hypoxic conditions in rats.Various doses
(0.03; 0.1; 0.3 or 1.0 mg//kg) of ifenprodil were administered to the
experimental rats i.p. Thirty min. later the passive avoidance task was
performed according to the step-through procedure. Immediately after the
training trial the animals were subjected to the period of oxygen
deprivation hypoxia. Rats were placed into hypoxia cage and the percentage
of oxygen was gradually reduced and continuously measured. The level of 3.5
V% of oxygen was reached in about twenty min. and maintained up to the lost
of righting reflex. Twenty four hours later passive avoidance retention was
tested. It has been found that ifenprodil succesfully antagonized the
memory deficit in hypoxia exposed rats. Namely, all tested doses of
mentioned drug led to a significant enhancement of the passive avoidance
behavior. The results of our experiments indicate that ifenprodil possess a
protective action against the impairment of passive avoidance retention
caused by hypoxia in rats. Our findings indirectly confirm the hypothesis
that memory decline in hypoxic conditions is associated with disturbances
in Ca2+ homeostasis.
Keywords: hypoxia, brain, ifenprodil, behavior, rat
- Type of paper
: Summary in proceedings
Title: EFFECT OF NIMODIPINE ON EEG ACTIVITY AND EEG POWER SPECTRA
IN KAINIC ACID-INDUCED SEIZURES IN RATS
- Authors:
- Križ, Jasna
- Župan, Gordana (142160)
- Križ, Mladen
- Simonić, Ante (43202)
Proceedings title: FINAL PROGRAM AND ABSTRACT BOOK
Language: engleski
Place: Milano, Italija
Year: 1994
Pages: from 59
Meeting: 6th INTERNATIONAL SYMPOSIUM ON PHARMACOLOGICAL CONTROL OF CALCIUM AND POTASSIUM HOMEOSTASIS Biological, Therapeutical and Clinical Aspects
Held: from 10/04/94 to 10/06/94
Summary: The aim of this study was to investigate whether nimodipine
could prevent kainic acid (KA)-induced changes in EEG activity.
Experimental animals were anesthetized and placed in a stereotaxic
apparatus. Vehicle or nimodipine (30 mg/kg) had been given 30 min. prior to
intahippocampal injection of KA (15 nmol). In each rat four screw
electrodes were placed over the frontal and parietal cortex. The EEG was
recorded in basal conditins and 5, 10, 15, 30 and 60 min. after KA
administration. In order to obtain EEG samples for frequency analysis, the
output from the EEG machine was amplified and was transmitted to an IBM
AT-compatible microcomputer via analog-to-digital converter. The system was
calibrated using 5OO uV. Two 7.5 s samples of artefact free EEG were
analyzed. In order to obtain power spectra between 0-30 Hz, spectral
averages were computed using Fast Fourier Transform. Individual EEG power
of each frequency bin was normalized to average total power of baseline.
EEG frequecies were collapsed into 1 Hz bins.Our results demonstrate that
nimodipine was effective in the prevention of changes in EEG activity
induced by intrahippocampal KA administration.
Keywords: kainic acid, epilepsy, EEG activity, EEG power spectra, nimodipine, rat
- Type of paper
: Summary in proceedings
Title: EFFECTS OF NICARDIPINE ON THE BRAIN FREE ARACHIDONIC ACID
LEVEL AND PASSIVE AVOIDANCE BEHAVIOR IN HYPOXIA-TREATED RATS
- Authors:
- Mršić, Jasenka (142272)
- Eraković, Vesna (900727)
- Župan, Gordana (142160)
- Križ, Jasna (201556)
- Simonić, Ante (43202)
- Varljen, Jadranka (85515)
Proceedings title: FINAL PROGRAM AND ABSTRACT BOOK
Language: engleski
Place: Milano, Italija
Year: 1994
Pages: from 59
Meeting: 6th INTERNATIONAL SYMPOSIUM ON PHARMACOLOGICAL CONTROL OF CALCIUM AND POTASSIUM HOMEOSTASIS Biological, Therapeutical and Clinical Aspects
Held: from 10/04/94 to 10/06/94
Summary: The influence of nicardipine on the brain free arachidonic
acid (FAA) level and on the learning ability in hypoxia-exposed rats was
examined. The experimental animals were injected with various doses (0.03;
0.1; 0.3; 1.0 mg/kg) of nicardipine. Thirty min. later some animals were
subjected to the period of oxygen deprivation hypoxia until the lost of the
righting reflex. Fifteen min. later they were decapitated and brains were
frozen in liquid nitrogen. The level of the brain FAA was quantified by gas
chromatography. The learning ability of all other animals pretreated with
the calcium channel blocker used, was tested in a passive avoidance task
according to the step-through procedure. These animals were exposed to the
hypoxic conditions immediately after the learning trial response had been
aqiured. A passive avoidance retention test was performed 24 hours later.
It was found that nicardipine did not influence the content of the brain
FAA, but significantly improved the retention deficits in the animals
exposed to hypoxia.
Keywords: hypoxia, brain, nicardipine, arachidonic acid, behavior,rat
- Type of paper
: Summary in proceedings
Title: THE INFLUENCE OF AMLODIPINE ON THE BRAIN FREE FATTY ACID
LEVEL IN PENICILLIN-INDUCED SEIZURES IN RATS
- Authors:
- Eraković, Vesna
- Križ, Jasna
- Župan, Gordana (142160)
- Mršić, Jasenka (142272)
- Simonić, Ante (43202)
- Varljen, Jadranka (85515)
Proceedings title: FINAL PROGRAM AND ABSTRACT BOOK
Language: engleski
Place: Milano, Italija
Year: 1994
Pages: from 58
Meeting: 6th INTERNATIONAL SYMPOSIUM ON PHARMACOLOGICAL CONTROL OF CALCIUM AND POTASSIUM HOMEOSTASIS Biological, Therapeutical and Clinical Aspects
Held: from 10/04/94 to 10/06/94
Summary: The aim of this study was to investigate the effect of the
calcium channel blocker, amlodipine, on the brain free fatty acid (FFAs)
level in penicillin-induced seizures in rats. The study was carried out on
Hannover-Wistar rats.The animals were anesthetized and placed in a
stereotaxic apparatus. Each of them received an injection of penicillin
(5000 U/5ul) into the left lateral ventricle. Various doses of amlodipine
(1; 3; 10 or 30 mg/kg) had been injected i.p. 30 min. prior to penicillin
application. The rats were decapitated five min. after the appearance of
epileptic seizures. FFAs were quantified by gas chromatography. Our results
demonstrate that intracerebral penicillin administration induced
significant increase of the brain FFAs level. It has also been found that
amlodipine did not prevent penicillin-induced accumulation of the brain
FFAs.
Keywords: penicillin-induced seizures, brain, free fatty acids, amlodipine, rat
- Type of paper
: Summary in proceedings
Title: THE INFLUENCE OF INDOMETHACIN ON THE BRAIN FREE FATTY ACID
POOL IN HYPOXIA-EXPOSED RATS
- Authors:
- Eraković, Vesna (900727)
- Varljen, Jadranka (85515)
- Župan, Gordana (142160)
- Mršić, Jasenka (142272)
- Križ, Jasna (201556)
- Simonić, Ante (43202)
- Milin, Čeda (70123)
Proceedings title: HB 94
Language: engleski
Place: Opatija, Hrvatska
Year: 1994
Pages: from 46
Meeting: ANNUAL MEETING OF CROATIAN BIOCHEMISTS WITH INTERNATIONAL PARTICIPATION
Held: from 10/14/94 to 10/15/94
Summary: The aim of this study was to determine the influence of
indomethacin, cyclo-oxygenase inhibitor, on free fatty acids (FFAs) pool in
hypoxia-exposed rats. The study was carried out on Hannover-Wistar rats
weighing 250 g. The animals of control group were intact, drug naive. All
other rats were subjected to a period of oxygen deprivation hypoxia until
loosing the righting reflex and decapitated 15 and 60 min. later. Two
groups of animals were injected with vehicle solution. Other two groups
were pretreated with 10 mg/kg of indomethacin, i.p. 30 min. before hypoxia
exposure. FFAs were quanitified by gas chromatography using internal
standard method. Our results demonstrate that indomethacin significantly
increases total FFAs level in hypoxic rats decapitated 15 min. after the
hypoxia-exposure, with no changes in free arachidonic acid content. On the
contrary, the increase in the brain free arachidonic acid level and no
change in total FFAs level was observed in hypoxic animals decapitated 60
min.after hyxpoxia.
Keywords: hypoxia, brain, free fatty acids, indomethacin, rat
- Type of paper
: Summary in proceedings
Title: EFFECT OF AMLODIPINE ON EEG ACTIVITY AND EEG POWER SPECTRA
IN PENICILLIN-INDUCED FOCAL SEIZURES IN RATS
- Authors:
- Simonić, Ante (43202)
- Križ, Jasna
- Župan, Gordana (142160)
Proceedings title: PHARMACOLOGICAL RESEARCH suppl., vol. 31
Language: engleski
Place: London, Velika Britanija
Year: 1995
ISBN/ISSN: 1043-6618
Pages: from 347
Meeting: FIRST EUROPEAN CONGRESS OF PHARMACOLOGY
Held: from 06/16/95 to 06/19/95
Summary: The purpose of our study was to investigate whether
amlodipine could prevent penicillin-induced excitatory changes in EEG
activity. Experiments were performed on adult Wistar rats. They were
anesthetized and placed in a stereotaxic apparatus. Vehicle or amlodipine
(1; 3; 10 or 30 mg/kg) had been given 30 min. prior to penicillin injection
(2000 I.U.) into the left motoric cortex. In each rat four screw electrodes
were placed over the left or right frontal and parietal cortex. The EEG was
recorded in basal conditions and 5, 10, 15, 30 and 60 min. after penicillin
administration. The output from EEG machine was amplified and was
transmitted to an IBM AT-compatible computer. Two 7.5 samples of artefact
free EEG were analyzed. In order to obtain power spectra between 0-30 Hz
spectral averages were computed using Fast Fourier Transform. Individual
EEG power of each frequency bin was normalized to average total power
baseline. EEG frequencies were colapsed into 1 Hz bins. Our results
demonstrate that amlodipine in doses of 10 and 30 mg/kg prevented the rise
of EEG power spectra induced by focal penicillin administration.
Keywords: penicillin-induced focal seizures, EEG activity, EEG power spectra, amlodipine, rat
- Type of paper
: Summary in proceedings
Title: EFFECTS OF NIMODIPINE AND MK-801 ON BEHAVIOR IN
HYPOXIA-EXPOSED RATS
- Authors:
- Župan, Gordana (142160)
- Mršić, Jasenka (142272)
- Simonić, Ante (43202)
Proceedings title: PHARMACOLOGICAL RESEARCH suppl., vol. 31
Language: engleski
Place: London, Velika Britanija
Year: 1995
ISBN/ISSN: 1043-6618
Pages: from 265
Meeting: FIRST EUROPEAN CONGRESS OF PHARMACOLOGY
Held: from 06/16/95 to 06/19/95
Summary: In this study we examined the influence of nimodipine and
MK-801 on the retention of passive avoidance in rats exposed to hypoxia.
The study was carried out on Hannover-Wistar rats weighing 250 g. Various
doses (0.03; 0.1; 0,3 mg/kg) of nimodipine or MK-801 were administered i.p.
Thirty min. later the passive avoidance task was performed according to the
step-through procedure. Immediately after the training trial the animals
were subjected to the period of oxygen deprivation hypoxia. Twenty four
hours later passive avoidance retention was tested. It was found that
hypoxia strongly impaired the retention of the passive avoidance response.
Nimodipine significantly improved the retention deficits in hypoxia-exposed
rats while MK-801 was uneffective.
Keywords: hypoxia, passive avoidance behavior, nimodipine, MK-801, rat
- Type of paper
: M.A.
Title:
Faculty: Medicinski Rijeka
Language: hrvatski
- Type of paper
: M.A.
Title: THE INFLUENCE OF THE CALCIUM CHANNEL BLOCKERS ON THE LEVEL
OF THE BRAIN FREE FATTY ACIDS IN EXPERIMENTAL MODELS OF CEREBRAL HYPOXIA
AND EPILEPSY IN RATS
Faculty: Medicinski Rijeka
Author: ERAKOVIĆ VESNA
Date of defense: 03/13/95
Language: hrvatski
Number of pages: 96
Summary: In this master thesis a) the influence of hypoxia and
penicillin-induced epileptic seizures on the level of the brain free fatty
acids and b) the effects of dihydropyridine calcium channel blockers on the
level of mentioned brain biochemical parameters in experimental models of
hypoxia and epilepsy have been examined. Animals were exposed to controlled
hypoxia conditions and were decapitated immediately, 5, 15 or 60 minutes
after the loosing of righting reflex.Prior to hypoxia exposure some rats
did not receive any substance, while the animals of control group received
vehicle solution. All other rats received variuos doses (0.03; 0.1; 0.3 or
1 mg/kg)of nimodipine, nicardipine or amlodipine. In other groups of
animals epileptic seizures were induced by injection of penicillin (5000
IU) into the left lateral ventricle. Rats with penicillin-induced seizures
were decapitated immediately, 5 or 15 minutes after the appearance of
seizures. Prior to penicillin application some rats did not receive any
substance, while the animals of control group received vehicle solution.
All other rats received tested doses (1; 3; 10 or 30 mg/kg) of mentioned
calcium channel blockers. The brains of all decapitated animals were taken
out and were frozen in liqiud nitrogen. The free fatty acids were
quantified using gas chromatography method. The results indicate that
hypoxia and penicillin-induced seizures cause the increase of the brain
total free fatty acid level and free palmitic, stearic, oleic and
arachidonic acid level.In both experimental models free arachidonic acid
showed the greatest increase in relation to its control level and in
comparison with the increase in levels of other free fatty acids. Tested
doses of nicardipine and amlodipine do not influence the increase of the
brain free fatty acid level in neither of these two experimental models.
Nimodipine prevents the increase of free arachidonic acid in hypoxia
exposed rats and both total brain free fatty acid and free arachidonic acid
level in rats with penicillin-induced seizures. The effects of nimodipine
are dose dependent. The results of this master thesis could contribute to
understanding of brain damage mechanisms caused by hypoxia and epilepsy and
to more effective treatment of mentioned disturbances.
Keywords: cerebral hypoxia, epilepsy, brain free fatty acids, calcium channel blockers, rat
- Type of paper
: Mentorship
Title: EFFECTS OF THE CALCIUM CHANNEL BLOCKERS ON PARTIAL AND
GENERALIZED EPILEPSY IN RATS
Faculty: Medicinski Rijeka
Mentor: ŽUPAN GORDANA
Date of defense: 05/11/95
Number of pages: 90
Author: Križ mr.sc. Jasna
Degree level: M.A.
- Type of paper
: Mentorship
Title: THE INFLUENCE OF THE CALCIUM CHANNEL BLOCKERS ON THE BRAIN
FREE FATTY ACIDS LEVEL IN EXPERIMENTAL MODELS OF CEREBRAL HYPOXIA AND
EPILEPSY IN RATS
Faculty: Medicinski Rijeka
Mentor: ŽUPAN GORDANA
Date of defense: 03/13/95
Number of pages: 96
Author: Eraković mr.sc. Vesna
Degree level: M.A.
- Type of paper
: Mentorship
Title:
