- Type of paper
: Book
Title: Vitamins and hormones - production and applications
- Authors:
- Kniewald, Zlatko (71903)
- Editors
- Šikić, Anita
Publisher: Hrvatska sveučilišna naklada
Year: 1993
Number of pages: 270
Number of references: 34
Language: hrvatski
Summary: The purpose of this textbook is to give the basic knowledge
tothe graduate and postgraduate students of biotechnology andother close
related fields in chemistry and technology ofvitamins and hormones. The
book comprehends four major topics:
Introduction with the basic knowledge of structure, nomenclatureand major
characteristics of vitamins and hormones as well asrelated compounds of
pharmaceutical importance; Vitaminsaccording to their physical and chemical
characteristics, production and applications; Hormones according to
theirphysiological characteristics, production and applications;
Majorspecific technological procedures for the production of vitaminsand
hormones. Particular importance is directed tobiotransformation of steroid
molecules which substitute expensivechemical conversions that are
environmentally undesirable. As aseparate chapter it is given example for
the future applicationsof growth hormone in meat and milk production as
well asapplications of vitamins and hormones in production ofbiologically
active proteins using animal cells in culture.
Keywords: biotehnology, vitamins, hormones, chemical constitution, biological activity, prohormones, analogues, receptors, insect hormones, plant hormones, biological conversion of steroids, process modelling,
Other: Sveučilišni udžbenik odobren od Odbora za znanstveno-nastavnu
literaturu Sveučilišta u Zagrebu rješenjem br. 02-362/4-1992. od srpnja
1992. a novčano potpomognuto sredstvima Ministarstva znanosti Republike
Hrvatske i PLIVE d.d. Zagreb
- Type of paper
: Book
Title: Methodology of Scientific Work
- Authors:
- Kniewald, Jasna (21481)
- Editors
- Repar, Željka
Publisher: Multigraf d.o.o.
ISBN: 953-6060-01-9
Year: 1993
Number of pages: 127
Number of references: 15
Language: hrvatski
Summary: University textbook "Methodology of Scientific Work" is
assignedto a purpose for the graduate and postgraduate students ofFaculty
of Food Technology and Biotechnology at University ofZagreb. The textbook
comprehends methodology of scientific workintroducing readers into major
prooblems of scientific work,that are common for all scientific
disciplines. The mostimportant chapters are: Importance and meanings of
scientificwork, Selection of topics for research, Overview and
descriptionof literature searching, Working hypothesis, Planning
ofresearch, Results evaluation, Making a manuscript, Writing ascientific
paper, Project proposal preparation, How to evaluate scientific papers.
Keywords: science, scientific work, scientist, research classification, scientific disciplines, scientific fields, methodology of research, literature search, data bases, CD-ROM, working hypothesis, project evaluation, modeling of processes, results evaluation, writing of scientific papers.
- Type of paper
: Paper in book
Title: Food production and pesticide influence - Implementation
of biotechnological procedure
- Authors:
- Kniewald, Jasna (21481)
- Kniewald, Zlatko (71903)
- Editors
- Aničić, Dražen
Publisher: Hrvatska akademija tehničkih znanosti
Year: 1995
Number of references: 12
Language: hrvatski
Summary: Increase of food production was followed with the
simultaneous production and increased application of agrochemicals. Due
tothis and other reasons, in the future, crop protection should be
organized only at that time when it is strictly necessary. As an
alternative to the nonorganized application of pesticides there exists an
Integrated Pest Management (IPM) program. Biotechnology with the
application of genetic engineering in the future will provide a new
generation of plants resistant to the different diseases and also apply
technology that will prevent a growth of certain weeds and asure a
significant quantities of energy from the renowable sources.
Keywords: food production, pesticide application, biotechnological solutions
- Type of paper
: Paper in journal
Title: Androgen hydroxysteroid dehydrogenases under the influence
of pyridoxine derivatives
- Authors:
- Kniewald, Zlatko (71903)
- Zechner, Vesna
- Kniewald, Jasna (21481)
Journal: Endocrine Regulations
Number: 1
ISSN: 0013-7200
Volume: 26
Year: 1992
Pages: from 47 to 51
Number of references: 12
Language: engleski
Summary: The metabolism of testosterone in the rat ventral
prostate,anterior pituitary, basal hypothalamus and amygdala was studied in
vitro under the influence of vitamin B6 compounds. The influence of these
compounds on the activity od 5 alpha-reductase (5R), 3alpha- and
17beta-hydroxysteroid dehydrogenase (3HSD,17HSD) was determined for all the
examined tissues. Pyridoxine hydrochloride significantly increased the
activity of 5R, 3HSD and 17HSD, but pyridoxal hydrochloride had an
inhibitory influence on 5R and showed no effect on 3HSD activity at the
prostate level. Male rat anterior pituitary, basal hypothalamus or amygdala
incubated with pyridoxal phosphate and pyridoxal hydrochloride showed
modified enzymatic activities. Pyridoxal hydrochloride showed an inhibitory
effect on 5R in the rat pituitary and basal hypothalamus as well as in rat
prostate.
Keywords: vitamin B6, testosterone metabolism, 5 alpha-reductase,hydroxysteroid dehydrogenases, in vitro, hypothalamus, pituitary, prostate, rat,
- Type of paper
: Paper in journal
Title: Effect of pesticides on oestradiol-receptor complex
formation in rat uterus cytosol
- Authors:
- Težak, Živana
- Šimić, Branimir (76144)
- Kniewald, Jasna (21481)
Journal: Food and Chemical Toxicology
ISSN: 0278-6915
Volume: 30
Year: 1992
Pages: from 879 to 885
Number of references: 22
Language: engleski
Summary: The effect of gama-1,2,3,4,5,6-hexachlorocyclohexane
(lindane),2-chloro-4-ethylamino-6-isopropylamino-s-triazine (atrazine)
and2-methylthio-4,6-bis-isopropylamino-s-triazine (prometryne) onthe
formation of a specific oestradiol-receptor complex in therat uterus
cytosol has been examined in vitro and in vivo. Bothin vitro and in vivo
the pesticides significantly inhibited theformation of the complex in the
rat uterus cytosol. The decreasein the number of free specific binding
sites on the receptors wasdetermined. The affinity of binding was not
modified under theinfluence of pesticides, and the Kd value was of the same
orderof magnitude. The inhibition was found to be fullynon-competitive.
Keywords: pesticides, oestradiol-receptor complex, rat, uterus, non-competitive inhibition
- Type of paper
: Paper in journal
Title: Possibility and Need for the Accelerated Application of
Mammalian Cells Biomass
- Authors:
- Kniewald, Zlatko (71903)
Journal: Prehrambeno-tehnološka i biotehnološka revija
ISSN: 0352-9193
Volume: 31
Year: 1993
Pages: from 107 to 109
Number of references: 4
Language: hrvatski
Summary: Development of molecular biology and molecular genetics
induceddevelopment of recombinant DNA technology called geneticengineering.
At the very first moment due to the fast growingmicrobes were the proper
solution for the production ofdifferent biologically active compounds.
Today there are known adifferent limitations in the application of microbes
for theproteins production. Therefore, research on the application
ofdifferent animal cells have been continued. The animal cellsbesides for
the production of diagnostics are more and moreapplied and for the
production of valuable pharmaceuticalmacromolecules. Besides of vaccines,
that are a bigest portion ofindustrial products based on animal cells,
different of otherbiologically active compounds like hormones,
enzymes,antibodies or cytokines are produced with the same technique.There
is a list of different examples where this production istoday and why need
to be developed in the future.
Keywords: animal cells, technology r-DNA, technology of animal cells, vaccines, erythropoietin, monoclonal antibodies, BHK, CHO,
- Type of paper
: Paper in journal
Title: Environmental impact, healthful food and education in
toxicology
- Authors:
- Kniewald, Jasna (21481)
- Kniewald, Zlatko (71903)
Journal: Central European Journal of Public Health
Number: 3
Volume: 3
Year: 1995
Pages: from 163 to 168
Language: engleski
Summary: Following the informative regional development and
environmental impacts in the Republic of Croatia, authors present the
problems emphased in environmental protection and health protection, and
present the status of the contamination of food and drinking water in 1993.
The level of education in the function of environmental protection in
Croatia and its importance are discussed.
Keywords: environmental health, Croatia, nutrition
- Type of paper
: Paper in proceedings
Title: Toxic effects of s-triazine herbicides as environmental
contaminants and the importance of their residues determination in food
- Authors:
- Gojmerac, Tihomira
- Kniewald, Jasna (21481)
Proceedings title: Zbornik radova VIII Savjetovanja o aktualnim zadacima veterinarske djelatnosti
Language: hrvatski
Place: Ohrid, B.J.R. Makedonija
Year: 1991
Pages: from 33 to 34
Meeting: VIII Savjetovanje o aktualnim zadacima veterinarske djelatnosti u zaštiti higijenske ispravnosti namirnica
Held: from 05/21/91 to 05/23/91
- Type of paper
: Paper in proceedings
Title: Pesticide influence on pubertal female reproductive system
- Authors:
- Kniewald, Jasna (21481)
- Težak, Živana
- Šimić, Branimir (76144)
- Kniewald, Zlatko (71903)
- Editors
- Battaglia, Robert
Proceedings title: Proceedings on 'Effects of Food on the Immune and Hormonal Systems'
Language: engleski
Place: Zurich, Švicarska
Year: 1991
ISBN/ISSN: 3-906516-02-4
Pages: from 170 to 174
Meeting: Effects of Food on the Immune and Hormonal Systems
Held: from 05/23/91 to 05/24/91
Summary: The in vitro and in vivo influence of atrazine, prometryne
andlindane on testosterone metabolism in pubertal female ratpituitary has
been examined. Pesticides significantly inhibitedthe in vitro formation of
5 alfa-dihydrotestosterone, but theformation of androst-4-ene-3,17-dione
was affected only in thepresence of atrazine. The in vitro addition of
atrazine andprometryne as an equimolar mixture exerted high inhibitory
effecton the 5 alpha-reductase activity. The in vivo treatment withlindane
(6 mg/100 g bw) or atrazine (12 mg/100 g bw) daily for 7days stimulated the
activities of 5 alpha-reductase and 17beta-hydroxysteroid dehydrogenase.
Keywords: reproduction, steroid hormones, puberty, 5 alpha-reductase, atrazine, pesticides, in vitro, in vivo, lindane,
- Type of paper
: Paper in proceedings
Title: Employment of animal cells in the manufacture of vaccines
- Authors:
- Kniewald, Zlatko (71903)
- Čajavec, Stanko
- Zechner, Vesna
- Gmajnički, Ines
- Markuš-Cizelj, Ljiljana
- Editors
- Hajsig, Danko
Proceedings title: Vakcinologija danas i sutra
Language: hrvatski
Place: Zagreb
Year: 1995
ISBN/ISSN: 953-96567-0-2
Pages: from 67 to 75
Meeting: Vakcinologija danas i sutra
Held: from 12/01/94 to 12/01/94
Summary: Employment of cells in the suspension for preparation of
viral vaccine against pseudorabies (Aujeszky's desease), under laboratory
conditions, has determined the parameters which indicated manufacturing
potentials. The cell line BHK 21 (C-13) was selected owing to its
applicability under standard laboratory conditions, and a high biological
activity was achieved.
Keywords: animal cells, submerse cultivation, BHK 21 C13, vaccines, bioreactors
- Type of paper
: Paper in proceedings
Title:
- Type of paper
: Summary in proceedings
Title: Reproductive performance in rat under atrazine influence
- Authors:
- Kniewald, Jasna (21481)
- Šimić, Branimir (76144)
- Kniewald, Zlatko (71903)
Proceedings title: EUROTOX '93
Language: engleski
Place: Copenhagen, Denmark
Year: 1993
ISBN/ISSN: 87-16-15050-3
Pages: from 131 to 131
Meeting: EUROTOX '93
Held: from 06/30/93 to 07/03/93
Summary: Effect of atrazine on rat ovarian cycling, male sexual
activity,on litter size and on the rate of conception during the mattingof
Fo and F1 generation were examined. Seven-days treatment withatrazine
hindered normal body weight gain in females and males.Atrazine induced
transient prolongation of estrous cycle. Fourweeks after treatment prostate
and seminal vesicles werereduced, while an increase of ovaries and
pituitary were detectedin females.
Defaults of conception indicates that atrazine influence thereproduction in
rats when both males and females were pre-exposed(Fo generation). Atrazine
affects the ovarian cycling byinterfering with endogenous hormone
equilibrium and physiologicalaction of hormones.
Keywords: atrazine, reproduction, in vivo treatment, estrous cycle, male sexual activity, litters, conception, mating, rat
- Type of paper
: Summary in proceedings
Title: Activin-A on secretion and release of pituitary hormones
- Authors:
- Bolt, Douglas
- Caldwell, Douglas
- Zechner, Vesna
- Editors
- Bazer, Fuller W.
Proceedings title: Society for the Study of Reproduction, supplement No.1, Biology of Reproduction
Language: engleski
Place: Champaign, Illinois, USA
Year: 1991
Pages: from 142 to 142
Meeting: 24th Annual Meeting of Society for the Study of Reproduction
Held: from 07/29/91 to 07/31/91
Summary: Activin-A, a homodimer of inhibin betaA-subunits, when
added toprimary cultures of rat pituitary cells is known to
increasesynthesis and Gn-RH-induced release of FSH. In this study
weinvestigated the interaction of activin-A and varioussecretogogues of
pituitary cells in culture. Cells were dispersedwith collagenase and plated
0,5x10 6cells/well (Day 0). Changesin hormone concentration in medium from
cells cultured with andwithout activin-A were of similar magnitude. Data
for hormonecontent in non-activin-A treated cells revealed a
significantreduction in FSH and LH by bFF; in LH, GH, and PRL by TRH; and
inGH by GHRh. Data indicate activin-A promotes release of FSH bycultured
ovine pituitary cells and blunts depletion in cellcontent of FSH, LH, GH,
and PRL induced by bFF, TRH or GHRh.
Keywords: pituitary hormones, FSH, LH, GnRh, GHRh, ovine pituitary, activin-A
- Type of paper
: Summary in proceedings
Title: Molecular mechanisms of steroid hormone action and
pesticide influence
- Authors:
- Kniewald, Jasna (21481)
- Kniewald, Zlatko (71903)
Proceedings title: Annual Meeting of Croatian Biochemists with international participation
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 68 to 68
Meeting: Godišnji Sastanak Hrvatskih Biokemičara - HB93
Held: from 06/17/93 to 06/18/93
Summary: Endocrine secretion into blood stream for action at
distantlocations is typical for hormones in the classical sense of theword.
Paracrine secretion is characteristic for neurotransmittersand
neuropeptides. Such signals are endogenous, while exogenousare chemicals
such as drugs, toxins, antigens, odorants orpesticides and physical medium
such as light waves.
Over the past five decades pesticides have become an integralfactor of both
agriculture and public health. Their use isindispensable to developing
countries and those countriesstriving to feed their populations. The lipid
solubility of mostpesticides afford them a habitat in organs possesing high
fatcontents and the fact is that the organs of reproduction contentthe
significant amount of lipide while a steroid hormones arealso soluble in
lipids.
Keywords: steroid hormones, mechanism of action, pesticides, endocrine glands, secretion, feedback mechanisms, ecotoxicology, environment protection
- Type of paper
: Summary in proceedings
Title: Characterization of androgen receptors from rat and
porcine ventral prostate and influence of binding inhibitors
- Authors:
- Meštrović, Nevenka
- Šimić, Branimir (76144)
- Kniewald, Jasna (21481)
- Kniewald, Zlatko (71903)
Proceedings title: Annual Meeting of Croatian Biochemists with international participation
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 127 to 127
Meeting: Godišnji Sastanak Hrvatskih Biokemičara - HB93
Held: from 06/17/93 to 06/18/93
Summary: The synthetic androgen methyltrienolone known as R1881 has
beenused to charecterize androgen receptors from rat and porcineprostate.
Photoactivation of R1881 and androgen receptors enablecovalently linking
indispensable for characterization by SDS-PAGEand permits the
identification of receptor present at very lowabundance in crude cytosol
preparation. The effect of atrazine on5 alpha-DHT receptor complex
formation and on the binding ofR1881 to the androgen receptors in crude
ventral pig prostatecytosol as well as in the rat prostate cytosol has
shown thatthis compound interferes in the complex formation and reduces
thephysiological activity responsible for the reproduction.
Keywords: androgen receptors, ventral prostate, rat, porcine, R1881, DHT-receptors, photoactivation, gel electrophoresis, cytosol
- Type of paper
: Summary in proceedings
Title: Growth factors influencing plating and seeding efficiency
of chick embryo fibroblasts
- Authors:
- Budak, Vesna
- Kniewald, Zlatko (71903)
Proceedings title: Annual Meeting of Croatian Biochemists with International Participation
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 130 to 130
Meeting: Godišnji Sastanak Hrvatskih Biokemičara - HB93
Held: from 06/17/93 to 06/18/93
Summary: The objective of the work was to use the low-protein
mediaTryptose Phosphate Broth (TPB) for supplementing a part of donorcalf
serum for the development of secondary culture of chickembryo fibroblasts.
Cultivation of chick embryo fibroblasts fromSPF (specific pathogenic free)
eggs was made on the 24 well, flatbottom with low evaporation lid, tissue
culture treatedpolystirene tissue culture plates. PE and growth rate
wasperformed 3 hours after the inoculation but before the beginningof
mithotic changes with 2x10 5 cells/mL. Duplication time offibroblasts was
detected (58-63 hours) in the exponential growthonly in the MEM+serum+TPB.
Keywords: animal cells, monolayer culture, plating efficiency, seeding efficiency, fibroblasts, chick embryo, duplication time, growth factors, MEM, TPB, HLY
- Type of paper
: Summary in proceedings
Title: Effect of 1-(6-purinyl)-2,5-dimethylpyrrole on the growth
of tobacco and Coleus Blumei Benth. callus culture
- Authors:
- Bajrović, Kasim
- Kniewald, Zlatko (71903)
- Vorkapić-Furač, Jasna (53001)
Proceedings title: Annual Meeting of Croatian Biochemists with International Participation
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 128 to 128
Meeting: Godišnji Sastanak Hrvatskih Biokemičara - HB93
Held: from 06/17/93 to 06/18/93
Summary: A cytokinin activity of new synthesized
compound1-(6-purinyl)-2,5-dimethylpyrrole on the growth of tobacco
andColeus Blumei Benth. callus culture (PDP) was experimentallytested using
the classical tobacco bioassay, shoots and callusculture of Coleus blumei
Benth. and compared to that of kinetin(Kt) as a standard cytokinin. The
results indicate that PDP hasa stimulating effect on the growth,
organogenesis andcaulogenesis as well as inhibitory effect on rhizogenesis
oftobacco and Coleus blumei Benth. callus culture and can beconsidered as
cytokinin i.e. a compound with plant growth hormonecharacteristics.
Keywords: plant cell culture, biotechnology, plant hormone, growth factors, callus, kinetin
- Type of paper
: Summary in proceedings
Title: 17 alpha-hydroxylation of progesterone using rat Leydig
cells in culture
- Authors:
- Gmajnički, Ines
- Nikšić, Vilma (72454)
- Wrischer, Mercedes
- Kniewald, Zlatko (71903)
Proceedings title: Annual Meeting of Croatian Biochemists with International Participation
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 128 to 128
Meeting: Godišnji Sastanak Hrvatskih Biokemičara - HB93
Held: from 06/17/93 to 06/18/93
Summary: The purpose of study was to hydroxylate progesterone using
asteroid 17 alpha-hydroxylase (E.C.1.14.99.9) present at Leydigcells. The
cells were isolated from the testis of 60 days oldrats and cultured in
Eagle's Midium Essential Medium with 10%horse serum. The cells were
cultured at 37 oC in a humidifiedatmosphere of 5%CO2 and 95% air at pH 7.4.
24 hours later Leydigcells were well attached to the surface of the flask,
whereascontaminating cells (Sertoli cells, germ cells) were
looselyattached. Such prepared cells contained more than 90% Leydigcells
and the final identification was carried out by electronmicroscopy. More
than 80% of progesterone was converted into 17alpha-hydroxyprogesterone
after 24 hours of incubation. In theconversion medium it was detected also
minor ammounts of otherprogesterone metabolites well separable by TLC and
columnchromatography. Achieved results suggest a simple procedure for17
alpha-hydroxilation of progesterone using Leydig cells inmonolayer culture.
Keywords: biotechnology, animal cells, monolayer culture, progesterone, steroid hormones, 17 alpha - hydroxyprogesterone, Leydig cells, Testis, rat
- Type of paper
: Summary in proceedings
Title: Influence of different growth promotors on BHK21 C13 cells
in suspension
- Authors:
- Zechner, Vesna
- Čajavec, Stanko
- Markuš-Cizelj, Ljiljana
- Kniewald, Zlatko (71903)
Proceedings title: Annual Meeting of Croatian Biochemists with International Participation
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 131 to 131
Meeting: Godišnji Sastanak Hrvatskih Biokemičara - HB93
Held: from 06/17/93 to 06/18/93
Summary: The aim of the study was to select the best growth promotor
forpropagation of BHK cells in laboratory scale. The work wascarried out
with a baby hamster kidney (BHK)21 clone 13 cellsuspension. Presence of
3%NBS + 5%TPB reduced the stacionaryphase and increased the growth of BHK
cells during the log. phasefor 80%. The same results were achieved if NBS
was supplementedwith horse serum.
Keywords: BHK cells, biotechnology, animal cells, cells in suspension, growth promotors, BHK21 C13,
- Type of paper
: Summary in proceedings
Title: Changes in protein sub-units in porcine anterior pituitary
and prostate effects of atrazine
- Authors:
- Osredečki, Višnja
- Kniewald, Jasna (21481)
Proceedings title: Annual Meeting of Croatian Biochemists with International Participation
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 132 to 132
Meeting: Godišnji Sastanak Hrvatskih Biokemičara - HB93
Held: from 06/17/93 to 06/18/93
Summary: The extensive use of herbicide atrazine, stipulate its
presenceon the farms close to the agricultural points, like
pig-breedingfarms. Therefore the purpose of this study was to resolve and
toidentify protein sub-units in porcine hormone dependent organs,anterior
pituitary and prostate, and further on to detectpossible changes in protein
subunits contents after in vitroatrazine influence. The significant
decrease in protein sub-unitMr 25000 was detected after addition of 0.088
to 0.70 umolatrazine for 9 to 66%. Atrazine concentration of 0.044
umolexerted no effects. At the same time protein sub-unit Mr 28500was
increased after addition 0.175 to 0.70 umol atrazine for7-77%, while 0.088
and 0.044 umol atrazine did not exert anychange.
Keywords: biotechnology, proteins, anterior pituitary, prostate, pesticides, atrazine, in vitro
- Type of paper
: Summary in proceedings
Title: Effects of s-triazine compounds on androgen-responsive
mechanisms in the pig pituitary
- Authors:
- Gojmerac, Tihomira
- Osredečki, Višnja
- Kniewald, Jasna (21481)
Proceedings title: Toxicology letters, supplement 1992
Language: engleski
Place: Roma, Italy
Year: 1992
Pages: from 155 to 155
Meeting: 6th International Congress of Toxicology
Held: from 06/28/92 to 07/03/92
Summary: s-triazine compounds, atrazine and prometryne are used
asselective herbicides in agriculture, leading to their presence inraw and
industrial food products.The influence of atrazine andprometryne on the
enzymic systems in the pig pituitary werestudied. Testosterone is converted
in the pig pituitary into itsmetabolites under the influence of steroid
converting enzymes.the in vitro addition of 0.7 umol atrazine or prometryne
did notchange the amount of 5 alpha-dihydrotestosterone formation
vs.control, but the formations of 3 beta-diol and androstenedionewere
significantly stimulated under the influence of atrazine vs.control values.
Addition of 0.7 umol prometryne did not affectthe tested enzyme activities,
but the addition of atrazine andprometryne in the mixture in equimolar
concentrationssignificantly reduced 3 beta- and 17 beta-HSD activities vs.
thesame molar concentration of atrazine as in the mixture.
Keywords: pesticides, biotechnology, s-atrazine, pig, pituitary, steroid conversion, hormone metabolism, testosterone, 5 alpha-reductase, 3 beta-hydroxysteroid dehydrogenase, 17 beta-hydroxysteroid dehydrogenase
- Type of paper
: Summary in proceedings
Title: Distribution of atrazine and its inlfuence on environment
- Authors:
- Kniewald, Zlatko (71903)
- Kniewald, Jasna (21481)
Proceedings title: Abstract from Symposium 'Ecologically rational development of chemical technolog
Language: engleski
Place: Zagreb
Year: 1991
Pages: from 367 to 367
Meeting: Symposium 'Ecologically rational development of chemical technologies' with the participation of the Alps-Adria Community of Regions
Held: from 02/14/91 to 02/15/91
Summary: Chemical pesticides for the control of agricultural pests
willcontinue to be required for the foreseeable future; although moreand
more they could be substituted with the new types of plantprotection.
Atrazine is inherently toxic substance to many forms of life andhis proper
and safe use must be based upon information drawn froma variety scientist
of disciplines, and its control is essentialto the well-being of society.
Inadequate education of peoples in the use and application ofpesticides
cause pollution of waterworks and wells. Long term useof atrazine and its
formulations in corn protection could at thecertain periods of years
particularly at the time of intensiveapplication - cause the penetration of
atrazine in waterworks andon this way on undesirable places where it can
cause theproblems. Our results show that atrazine together with
itsmetabolic compound deethylatrazine either in vitro or in vivoreduce the
enzyme activity of 5 alpha-reductase, 3 beta- and 17beta- hydroxysteroid
dehydrogenase at CNS level of all selectedspecies of mammals (rat, calf,
sheep) and birds (turkey,cockerel).
Keywords: atrazine, environment, distribution, animal reproduction, androgen hormones, toxicology, biotechnology, ecotoxicology, pesticides
- Type of paper
: Summary in proceedings
Title: Effects of organochlorine and s-triazine compounds on 5
alpha-reductase and androgen responsive mechanisms in the rat prostate
- Authors:
- Kniewald, Jasna (21481)
- Kniewald, Zlatko (71903)
- Editors
- Matias, Jonathan R.
Proceedings title: Androgens and Antiandrogens - chemistry, biology and pharmacology
Language: engleski
Place: Zagreb
Year: 1991
Pages: from 35 to 35
Meeting: Terra Symposia 'Androgens and Antiandrogens'
Held: from 05/28/91 to 05/31/91
Summary: The ventral prostate of the rat secretes relatively large
amountsof a androgen-binding protein whose synthesis isdihydrotestosterone
(DHT) - dependent, but it is different fromsteroid receptors. The amount of
DHT specific receptors in therat prostate and the number of the specific
binding sites are incorrelation with the activity of 5 alpha-reductase and
3alpha-hydroxysteroid dehydrogenase. The fully non-competitivetype of 5
alpha-reductase inhibition was determined in the ratprostate by the
application in vitro microquantities oforganochlorine (lindane and
malathion) and s-triazine (atrazineand prometryne) compounds. We can
predict that the increasedsynthesis of testosterone or decreased
elimination of theproduced DHT from the rat prostate could induce anabolic
effectthrough the increased activityof 3 alpha-HSD.
Keywords: organochlorine compounds, s-triazine, toxicology, 5 alpha-reductase, testosterone metabolism, dihydrotestosterone, androgen receptors
- Type of paper
: Summary in proceedings
Title: Pesticide influence on pubertal female reproductive system
- Authors:
- Kniewald, Jasna (21481)
- Težak, Živana
- Šimić, Branimir (76144)
- Kniewald, Zlatko (71903)
Proceedings title: Effect of Food on the Immune and Hormonal Systems
Language: engleski
Place: Zurich, Švicarska
Year: 1991
Pages: from 170
Meeting: Effects of Food on the Immune and Hormonal Systems
Held: from 05/23/91 to 05/24/91
Summary: The in vivo and in vitro influence of atrazine, prometryne
and lindane on testosterone metabolism in pubertal female rat pituitary has
been examined. Pesticides significantly
inhibited the in vitro formation of 5alpha-dihydrotestosterone, but the
formation of androst-4-ene-3,17-dione was affected only in the presence of
atrazine. The in vivo treatment with lindane (6 mg/100 g bw) or atrazine
(12 mg/100 g bw) daily for 7 days stimulated the activities of
5alpha-reductase and 17beta-hydroxysteroid dehydrogenase.
Keywords: atrazine, prometryne, lindane, female rat, pituitary, 5alpha-reductase
- Type of paper
: Summary in proceedings
Title: The herbicide atrazine inhibits androgen converting
enzymes and protein symthesis in male porcine pituitary
- Authors:
- Kniewald, Zlatko (71903)
- Osredečki, Višnja
- Kniewald, Jasna (21481)
Language: engleski
Place: Ann Arbor, SAD
Year: 1994
Meeting: 26th Annual Meeting of Society for the Study of Reproduction
Held: from 07/24/94 to 07/27/94
Summary: Proteins pattern (14.4-94 kD) in male porcine pituitary
cytosol was determined by SDS PAGE (1-D, 2-D) after Commssie blue staining
and quantified by UltroScan XL densitometer. Atrazine added to the
incubation medium at the range of concentrations from 0.175 micromol to 0.7
micromoles, inhibited significantly 5alpha-reductase. Pituirary cytosol was
prepared after tissue incubation and protein content of 24.7 kD present in
control 22,1%, was reduced for 67% after atrazine treatment. Protein
fraction 28.2 kD present in control 2.5% was increased for 79.9%. Purified
fraction 28.2 kD after gel filtration and 2-D SDS PAGE was identified as
prolactine.
Keywords: pituitary, male pig, protein content, atrazine
- Type of paper
: Summary in proceedings
Title: Pesticide toxicity for molecular mechanisms of steroid
hormone action
- Authors:
- Kniewald, Jasna (21481)
- Kniewald, Zlatko (71903)
Language: engleski
Year: 1994
Pages: from 43
Meeting: EUROTOX'94 (34rd European Congress of Toxicology)
Held: from 08/21/94 to 08/24/94
Summary: Toxicity of selected liposoluble pesticides in common use
(atrazine and lindane) were tested on the biochemical mechanisms
responsible for normal function of gonadotropic feedback mechanisms. In
vitro experiments showed that atrazine /A/ and lindane /L/ in subtoxic
concentrations markedly but with different intensity decrease: conversion
of T to DHT in rat prostate (A 5-23%, L 9-41%) in pituitary (A 4-21%, L
11-35%), in porcine pituitary (A 2-14%); formation of specific
5alpha-DHT-receptor complex in rat prostate (A 10-48%) and formation of
oestradiol-receptor complex in rat uterus (A 14-41%, L 11-42%).
Keywords: steroid hormones, pesticides, atrazine, lindane, 5alpha-reductase, DHT-receptor
- Type of paper
: Summary in proceedings
Title: Characterization of prolactin and growth hormone in
porcine pituitary
- Authors:
- Kniewald, Jasna (21481)
- Osredečki, Višnja
- Kniewald, Zlatko (71903)
Language: engleski
Year: 1995
Pages: from 68
Meeting: 7th European Congress on Biotechnology
Held: from 02/19/95 to 02/23/95
Summary: On 1-D SDS PAGE it was possible in porcine pituitary within
30 protein bands to detect nine main protein bands with % protein portion
from total protein content higher than 2%. Pituitary cytosol was purified
by gel filtration on Ac 54 with cytochrom C, alpha-chymotripsin and
ovalbumin as standards. Fractions containing proteins Mr 20-30 kD were
analysed by 1-D SDS PAGE. by introducing internal standards of prolactin
and growth hormone, on 2-D SDS PAGE we were able to detect that protein
band 28 kD corresponds to prolactine.
Keywords: prolactine, growth hormone, male pig, pituitary, protein contents
- Type of paper
: Summary in proceedings
Title: Progesterone 17alpha-hydroxylation in rat Leydig cells
- Authors:
- Kniewald, Zlatko (71903)
- Gmajnički, Ines
- Kniewald, Jasna (21481)
Language: engleski
Place: Nice, Francuska
Year: 1995
Pages: from 92
Meeting: 7th European Congress on Biotechnology
Held: from 02/19/95 to 02/23/95
Summary: In Leydig cells isolated from rat testis steroidogenesis
was followed using pregnanelone as a substrate. Pregnelenone is the first
converted to progesterone by the action of 3beta-hydroxysteroid
dehydrogenase followed by the activity of 17alpha-hydroxylase that
catalyses 17alpha-hydroxylation of progesterone and cleavage of two carbon
site-chain to yield the C19 steroid, androstendione. Conversion of
substrate was stimulated by LH, FSH or inhibited by cyproterone acetate and
for atrazine alternatively. Freezing and thawing of Leydig cells in
addition of glycerol yield with 100% of the total cells.
Keywords: Leydig cells, cell culture, steroidogenesis, pregnenolone, testosterone biosynthesis
- Type of paper
: Summary in proceedings
Title: Endogenous toxicity of pesticides from food
- Authors:
- Kniewald, Jasna (21481)
Language: engleski
Place: Zagreb
Year: 1994
Pages: from 135 to 136
Meeting: 2.Hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista (s međunarodnim sudjelovanjem)
Held: from 06/15/94 to 06/17/94
Summary: Over the past five decades pesticides have become an
integral factor of both agriculture and public health. The widespread use
of pesticides produces residues in meat, dairy products, egges and feed
crops, which have become a matter of concern in respect to human health.
Growing evidence suggests that the reproductive failure in females and the
unknown etiology of a number of cases of male infertility might be
attibuted to chronic exposure to pesticides via food. Pestides can
adversely affect hormonal balance in mammals: produce changes in steroid
dependent organs such as uterus or prostate gland, and central nervous
system.
Keywords: pesticides, food, endogenous influence
- Type of paper
: Summary in proceedings
Title: Atrazine inhibits protein synthesis in porcine pituitary
- Authors:
- Osredečki, Višnja
- Kniewald, Zlatko (71903)
- Bolt, Douglas
- Kniewald, Jasna (21481)
Language: engleski
Place: Zagreb
Year: 1994
Pages: from 82
Meeting: 2.Hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista (s međunarodnim sudjelovanjem)
Held: from 06/15/95 to 06/19/95
Summary: On 1-D SDS PAGE it was possible to detect in porcine
pituitary nine main protein bands with % of protein portion from total
protein content higher than 2%. The decrease in protein Mr 24.5 kD was
detected after addition of 0.88 to 0.7 mmol of atrazine for 9-66%, while at
the same time the increase in protein Mr 28 kD was 7-77%. By using internal
standards of prolactin and growth hormone, and introducing 2-D SDS PAGE, we
were able to detect that the increase in protein content corresponded to
prolactin and the decrease to growth hormone.
Keywords: atrazine, prolactin, growth hormone, pituitary, pig
- Type of paper
: Summary in proceedings
Title: Characterization of androgen receptors and influence of
atrazine
- Authors:
- Meštrović, Nevenka
- Šimić, Branimir (76144)
- Kniewald, Jasna (21481)
- Kniewald, Zlatko (71903)
Language: engleski
Place: Zagreb
Year: 1994
Pages: from 81
Meeting: 2.Hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista (s međunarodnim sudjelovanjem)
Held: from 06/15/94 to 06/17/94
Summary: The synthetic androgen methyltrienolone (R1881) and
5alpha-dihydrotestosterone (DHT) have been used to characterize androgen
receptors from rat ventral prostate. Characterization was performed in
vitro by SDS PAGE and isoelectric focusing (IEF) after photolabelling by
exposition of sample containing 3H-R1881 and androgen receptors within the
cytosol to UV light. Binding was detected at Mr 94 kD and 68 kD, and
isoelectric point was 4.6. Binding was blocked after addition of 1.75 mM of
atrazine for 70%.
Keywords: androgen receptors, prostate, reproduction, atrazine
- Type of paper
: Summary in proceedings
Title: Protein synthesis in rat male pituitary and hypothalamus
under atrazine and lindane influence
- Authors:
- Kniewald, Jasna (21481)
- Kniewald, Zlatko (71903)
Language: engleski
Place: Prag, Češka Republika
Year: 1995
Pages: from 47
Meeting: EUROTOX'95 (34rd European Congress of Toxicology)
Held: from 08/27/95 to 08/30/95
Summary: On 1-D 12% SDS PAGE it was possible in rat pituitary and
hypothalamus to detect seven main protein bands with % of protein portion
from total protein contents higher than 2%. In pituitary the decrease in
protein Mr 15.2 kD for 29.8% and Mr 24.7 kD for 18.3% were detected in
lindane in vivo treated rats, and in protein Mr 24.7 kD for 24% in atrazine
in vivo treated rats. In hypothalamus an increase in protein Mr 30.9 kD for
22.9% was detected in lindane in vivo treated rats, while with atrazine
influence was not statistical significant.
Keywords: rat, pituitary, atrazine, lindane, protein synthesis
- Type of paper
: Ph.D.
Title: The influence of activin-A and inhibin on gonadotropic
feedback mechanism and their influence on the 5alpha-reductase activity in
the mammalian pituitary cells
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Author: ZECHNER-KRPAN VESNA
Date of defense: 04/03/95
Language: hrvatski
Number of pages: 113
Summary: The use of pituitary cells in culture for studying the
regulation of pituitary hormone secretion provides a number of advantages
over in vivo experiments. Such cultures allow the isolation of the
pituitary from the neural and hormonal influences and the addition of the
test substances under a controlled environment. By using this experimental
model and pituitary cells in culture, the cell environment could be studied
and the secretion and cell content of hormones could be determined. The
higher 5alpha-reductase activity in the pituitary is the result of
disturbed amounts of activin-A and inhibin or it is the result of
insuficiency of endogene testosterone, and in castrated rats is in
correlation with the action of activin-A, which stimulates the synthesis
and release of FSH from the cells.
Keywords: rat, pituitary, gonadotropic mechanism, activin-A, inhibin, 5alpha-reductase
- Type of paper
: Ph.D.
Title: Regulatory mechanisms of mammalian gonadotropic systen
under the influence of organochlorine and s-triazine compounds
Faculty: Farmaceutsko-biokemijski fakultet Sveučilište u Zagrebu
Author: ŠIMIĆ BRANIMIR
Date of defense: 11/29/95
Language: hrvatski
Number of pages: 130
Keywords: rat, pituitary, hypothalamus, prostate, 5alpha-reductase, DHT-receptor complex, reproduction
- Type of paper
: M.A.
Title: Development of Leydig cells monolayer culture and its
application for determination of toxicants
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Author: GMAJNIČKI INES
Date of defense: 10/31/95
Language: hrvatski
Number of pages: 70
Keywords: Leydig cells, monolayer cell culture, biosynthesis of androgen hormones, inhibition of hormone biosynthesis
- Type of paper
: M.A.
Title: Interaction of pituitary and hypothalamic hormones on
pituitary cells in primary culture
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Author: ZECHNER-KRPAN VESNA
Date of defense: 03/06/92
Language: hrvatski
Number of pages: 113
Summary: Sheep pituitary cells in culture are capable to regulate
thesecretion of pituitary hormones after addition of differentstimulators
and inhibitors. In the part of this study weinvestigated the interaction of
inhibin, activin-A and varioussecretogogues of pituitary hormone
setretion/release using sheeppituitary cells in monolayer culture. The
study ofinfluence ofinhibin using pituitary cells showed inhibiting effect
onsynthesis of: FSH, cellular content of FSH and LH, as well ascontent of
GnRH receptors at the pituitary gland. Results in thisstudy confirm that
inhibin added to the culture of sheeppituitary cells after 1-3 days of
cultivation significantlyinhibits release of FSH. Sheep pituitary cells in
primary cellculture were subcultivated 3 time in 2 weeks. The main
metabolicpathway of testosterone in adult male rat neuroendocrine tissueis
followed by the catalytic influence of 5 alpha-reductase, 3alpha- and 17
beta-hydroxysteroid dehydrogenase activities. Theseenzymes are responsible
for the formation of 5 alpha-DHT, 3alpha-dion and androstenedione.
Keywords: biotechnology, animal cells, pituitary, 5 alpha-reductase, dihydrotestosterone, monolayer culture, neuroendocrine regulatory mechanisms, inhibin, activin-A, pituitary hormones, neurosecretion, neuroregulation, androgen hormones, B6 vitamins, NADP, prostate
- Type of paper
: Mentorship
Title: Characterization of androgen receptors by binding
metyltrienolone (R1881) and influence of atrazine on hormone receptor
complex formation
Faculty: postdiplomski studij prirodnih znanosti, područje kemija Sveučilište u Zagrebu
Mentor: KNIEWALD ZLATKO
Date of defense: 04/28/94
Number of pages: 79
Author: Meštrović Mr.dipl.inž. Nevenka
Degree level: M.A.
- Type of paper
: Mentorship
Title: Effects of 1-(6-purinyl)-2,5-dimethylpyrrole on the growth
of tobacco and decorative nettle culture
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: VORKAPIĆ-FURAČ JASNA
Date of defense: 11/11/92
Number of pages: 59
Author: Bajrović Mr. dipl. inž. Kasim
Degree level: M.A.
- Type of paper
: Mentorship
Title: Interaction of pituitary and hypothalamic hormones on
pituitary cells in primary culture
Faculty: Prehrambeno-biotehnološki fakultet Sveučilište u Zagrebu
Mentor: KNIEWALD ZLATKO
Date of defense: 03/06/92
Number of pages: 113
Author: Zechner Mr. dipl. inž. Vesna
Degree level: M.A.
- Type of paper
: Mentorship
Title: The response of central nervous system and uterine cells
in female rats on the influence of s-triazines and organochlorine compounds
Faculty: Prirodoslovno-matematički fakultet Sveučilište u Zagrebu
Mentor: KNIEWALD JASNA
Date of defense: 12/28/90
Number of pages: 158
Author: Težak Mr.dipl.inž. Živana
Degree level: M.A.
- Type of paper
: Technical report
Title: Submersed cultivation of animal cells
Ordering party: PLIVA-Zagreb
Institution depot: Prehrambeno-biotehnološki fakultet, Zagreb
Year: 1993
Number of pages: 10
Language: hrvatski
- Type of paper
: Project
Title: Pesticide behaviour in agricultural systems of Yugoslavia
- part Croatia, princ. invest. Zlatko Kniewald
Ordering party: SAD-JU zajednički fond (dio Hrvatska), PN 934
Year: 1991
Number of pages: 10
Language: engleski
- Type of paper
: Invited lecture
Title: Molecular mechanisms of steroid hormone action and
pesticide influence
Institution: Hrvatsko biokemijsko društvo
Year: 1993
- Type of paper
: Invited lecture
Title: Endogenous toxicity of pesticides from food
Institution: Prehrambeno-biotehnološki fakultet, Zagreb
Year: 1994
- Type of paper
: Invited lecture
Title:
- Type of paper
: Other
Title:
