SPECTROSCOPIC STUDIES OF BIOLOGICAL MACROMOLECULES
Main researcher
: PIFAT, MARGARETA (37174) Assistants
BRNJAS-KRALJEVIĆ, JASMINKA (5663)
KVEDER-ILAKOVAC, MARINA (113615)
NOTHIG-LASLO, VESNA (33526)
Type of research: basic Duration from: 01/01/91. to 12/31/93. Papers on project (total): 30
Papers on project quoted in Current Contents: 20
Institution name: Institut "Ruđer Bošković", Zagreb (98) Department/Institute: Department for Experimental Physics Address: Bijenička cesta 54 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (01) 4561-111
Summary: The structure and dynamics of phospholipids and
lipoproteins as model systems for the biological structure complexity, and
tissues as chosen biological systems, are studied by spectroscopic
methods (ESR, NMR). The surface structure properties of phospholipids and
lipoproteins are defined, i.e. the surface charge distribution, molecular
packing and lipid-protein interactions. Microscopic distribution and
metabolism of free radicals are studied in the liver tissue. Organic
paramagnetic centers induced by gamma-irradiation of aluminates and
aluminosilicates, and the copper II complexes with aminoacides are studied
by ESR, UV/VIS and IR spectroscopies.
Research goals: 1) In lipoproteins: to define the structure and
dynamic properties of lipoproteins from human plasma: a) surface charge
distribution, b) nature of lipid-protein interaction, 2) In the liver
tissue: to define the metabolism of nitroxides as the function of
diffferent parameters (concentration of oxygenand iron ions), 3) To
correlate the tissue growth and regeneration of the normal tissue with the
content and structure of lipoproteins, 4) To show that aminoacids form
stabile and long lived organic paramagnetic centers in the
gamma-irradiated aluminates and aluminosilicates. 5) To define the nature
of Cu(II) coordination sphere in the complexes with aminoacids as the
model systems for the transport of copper and its binding properties to
proteins.
COOPERATION - PROJECTS
Name of project
: NIH-PN757 Nucleic acids - membrane lipids
interactions Name of institution: University of Cincinnati, Chemistry Department City: Cincinnati, SAD
COOPERATION - INSTITUTIONS
Name of institution
: University of Cincinnati, Chemistry
Departement Type of institution: University/Faculty City: Cincinnati, SAD
Name of institution
: Institut "Jožef Stefan" Type of institution: University/Faculty City: 61000 - Ljubljana, Slovenija
Name of institution
: Institut za medicinska istraživanja i
medicinu rada Type of institution: University/Faculty City: 10000 - Zagreb, Croatia
Name of institution
: Karl-Franzens Universitat, Institut fur
Medizinische Biochemie Type of institution: University/Faculty City: Graz, Austrija
Name of institution
: Medicinski fakultet Sveučilišta u Zagrebu Type of institution: University/Faculty City: 10000 - Zagreb, Croatia Other information about the project.