SVIBOR - Project code: 1-07-168

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 1-07-168


Finding the optimal chromatographic separation


Main researcher: KAŠTELAN-MACAN, MARIJA (20421)


Assistants
Type of research: basic
Duration from: 01/01/91. to 01/01/95.
Papers on project (total): 119
Papers on project quoted in Current Contents: 18
Institution name: Fakultet kemijskog inženjerstva i tehnologije, Zagreb (125)
Department/Institute: Department of analytical chemistry
Address: Marulićev trg 19
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (041) 452 477
Fax: 385 (041) 450 725
Summary: The effect of interaction in thin-layer chromatographic process is described. The addition of Cu(II), Al(III) and Fe(III)-ions to silica gel when preparing the chromatographic layers proveds an oportunity for a very good separation of structural phenol isomers. It was found that phenol-metal interaction products and interaction products in the solvent system used as well as changed adsorption properties of impregnated silica gel play prominent roles in the TLC separation of phenols. Analytical procedure for the quantitative determination of Mg in Al-alloys using ion-exchange TLC is described. Since Mg(II) has lower distribution coefficient on sulphonated polysyrene resins in an acidic medium, very good resolution of Al-Mg mixture was achieved. The method of quantitative TLC determination of aquatic humic acids after preconcentration using XAD-resins was worked out. The conditions of biodegradation and biosorption of humic substances using fungal pellets were examined. Application of natural zeolites in the process of humic substances removal was investigated. Silver from the waste water was successfully isolated using ion-exchangers and concentrated by microorganisms. The possibility of ion-chromatography application in environmental analysis was described. Ion-chromatography was used in the study of ammonia removal from ground water by biological oxidation, isomorphus substitution of phosphorus into natural zeolite and reaction between the activated blast furnace slag and serias of anions. In order to optimise the crude oil components determination GC and HPLC were used. The mass spectra of same organic compounds were studied. Validation of the quantitative chromatographic analysis of amino acids on laboratory prepared plates was performed. A new procedure for the isolation and determination of radiactive strontium from natural samples and its separation from calcium by ion exchange was tested. The ion-exchange properties of some natural zeolites and their selectivity to heavy metal ions from waste waters were studied.

Keywords: thin-layer chromatography, ion-chromatography, modification of chromatographic layer, optimisation of chromatographic process, biosorption and biodegradation, humic and fulvic acids, metal ions, anions, phenol compounds

Research goals: Dramatical changes have taken place in chemical analysis during the last dekades. Most measurements are now being made using complex and sophisticated instruments. However, the overall uncertainty of analytical process is effected in the first place by sampling and preparation of sample error, respectively. The purpose of this investigation is to decrease the overall uncertainty by getting the representative sample and by finding out optimal preconcentration and separation conditions. In order to achieve better TLC separation, number of chromatographic layer modifications are going to be worked out. Metal-ions, ion-exchange resins and natural zeolites will be used as impregnants. The separation of the isomer compounds, humic and fulvic acids, metal ions and anions will be axamined. The anorganic ion content in water and soil will be monitored using ion-chromatography. Analyte preconcentration will be made using ion-exchange and adsorption resins. New technique of the quantitative TLC determination will be worked out. Conditions of pollutant biosorption and biodegradation using different microorganisms is going to be examined. In order to optimise the mentioned systems very good knowledge of reaction mechanisms is needed. From this investigation we expect new informations about the chromatographic process and its more successful control.

COOPERATION - PROJECTS


  1. Name of project: 2-14-034 Nove metode za oplemenjivanje tekstila
    Name of institution: Tekstilno-tehnološki fakultet
    City: 10000 - Zagreb, Croatia

  2. Name of project: 2-08-329 Izrada referentnih materijal za strojarsku industriju
    Name of institution: Fakultet strojarstva i brodogradnje
    City: 10000 - Zagreb, Croatia

  3. Name of project: 1-07-041 Istraživanje i razvoj tehnologija pročišćavanja voda
    Name of institution: Fakultet kemijskog inženjerstva i tehnologije
    City: 10000 - Zagreb, Croatia

  4. Name of project: 4-99-119 Biotehničke mogućnosti smanjenja emisije štetnih tvari u okolini
    Name of institution: Institut za razvoj i međunarodne odnose
    City: 10000 - Zagreb, Croatia
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Last update: 10/09/95
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