SVIBOR - Project code: 1-08-144

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 1-08-144


expression and role of oncogene and growth factors in malignant tumors


Main researcher: PAVELIĆ, KREŠIMIR (35262)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/93.
Papers on project (total): 154
Papers on project quoted in Current Contents: 49
Institution name: Institut "Ruđer Bošković", Zagreb (98)
Department/Institute: Division of Molecular Medicine Department of Biology and Medicine Ruđer Bošković Institute
Address: Bijenička cesta 54
City: 10000 - Zagreb, Croatia
Communication
Fax: 385 (01) 42 42 39
Phone: 385 (01) 4561 114
Phone: 385 (01) 42 42 39
Summary: The work on the project continues to be concerned with ananalysis of the mechanisms and role of oncogene activation and growth factors involvement in tumor growth. The research included the characterization of some growth factors-like molecules and cell-cycle regulatory molecules, including receptors, signal transducers, transcription factors. Some of the signals originating from the receptors need to enter the nucleus of the cells to activate particular genes. This interaction is mediated by specific proteins (nuclear transcription factors) that bind to region adjacent to the relevant genes. We have been undertaking extensive studies on following oncogenes and growth factor: c-myc, c-myb, c-H-ras, c-erbB-2/neu, EGF-R, PDGF, insulinoids,TGF alfa, TGF beta. Our studies have indicated that a critical abnormality involved in tumor development is acquistion by cells for an autocrine capacity to produce their own growth factors. Details are published in listed papers. Two major methodological improvements have been done: DNA amplifications by polymerase chain reaction from cytological smears and immunohistochemical localization of nuclear oncoproteins in paraffin embedded tissues. Gene therapy for solid tumors using herpes simplex thymidine kinase suicide gene has started. Details are published in listed papers. All goals of the project have been realized. The project have had significant impact on the development of molecular human genetic in Republic of Croatia.

Keywords: oncogenes, growth factors, tumors

Research goals: 1. identification of mutant and/or wilde type of oncoproteins intumor cells and tissues. Elucidation of their role in tumordevelopment. 2. izolation and characterization of some growth factors andreceptors from tumor tissues or cells which play a role incontrol of tumor growth. 3. modulation of expression of some protooncogenes. 4. DNA amplification by PCR from archival cytological smears andimprovent of a method for immunohistochemical detection ofnuclear oncoproteins. 5. pathogenesis of chronic lymphocytic leukemia. 6. the role of erbb familz of genes in human tumors. 7. the molecular aspects of tumor metastases. 8. gene therapy of cancer. 9. cellular and molecular mechanisms of newly synthesized anti-cancer drugs. 10. accumulation of multiple genetical alterations in human tumors. 11. the role of cell surface molecules in T-cell differentiation and function. 12. new approaches in molecular diagnostics. 13. automatic oligonucleotide synthesis. All goals have been realized in the period from 1991. to 1995.

COOPERATION - PROJECTS


  1. Name of project: >CI1*-0334 Biology of SICRI
    Name of institution: Commission of the European Communities, Directorate General for Science, Research and Development
    City: Brussels, Belgija

  2. Name of project: >CI1*-CT88-0335 Biology of SICRI
    Name of institution: Universitats-Krankenhaus Eppendorf, Institut fur Physiologische Chemie
    City: Hamburg, Njemačka

  3. Name of project: Oncogenes and tumor suppressor genes in human cancer
    Name of institution: University of Cincinnat, College of Medicine, Department of Otolaryngology and Maxillofacial Surgery
    City: Cincinnati, USA

  4. Name of project: 3-01-178 Formiranje banke tumora za bazična istraživanja
    Name of institution: Establishing of tumor bank for basic research
    City: 10000 - Zagreb, Croatia

COOPERATION - INSTITUTIONS


  1. Name of institution: Universitats-Krankenhaus Eppendorf, Institut fur Physiologische Chemie
    Type of institution: University/Faculty
    City: Hamburg, Njemačka

  2. Name of institution: Commission of the European Communities, Directorate General for Science Research and Development
    Type of institution: State administration
    City: Brussels, Belgija

  3. Name of institution: University of Cincinnati, College of Medicine, Department of Otolaryngology and Maxillofacial Surgery
    Type of institution: University/Faculty
    City: Cincinnati, USA

  4. Name of institution: Mayo Clinic and Foundation, Oncology Research
    Type of institution: International organization
    City: Rochester MN, USA

  5. Name of institution: Hrvatska akademija znanosti i umjetnosti
    Type of institution: University/Faculty
    City: 10000 - Zagreb, Croatia

OTHER ACHIEVEMENTS


  1. Name: Razvoj metoda molekulske dijagnostike primjenjene u humanoj genetici, infektologiji, ginekologiji
    Type of achievement: Other
    Authors: Krešimir Pavelić, Koraljka Gall-Trošelj, Magdalena Grce, Sonja Levanat, Jasminka Pavelić
Other information about the project.
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