- Type of paper
: Paper in journal
Title:
- Authors:
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
- Šterbić, Ivo
Journal: Ichthyologia
Number: 1
Volume: 23
Year: 1991
Pages: from 25 to 35
Language: engleski
Summary: Blood have been sampled by cardiac puncture from non
anaestetizedsea bass from commercial fish farming facilities in Limski
kanal(Center for Mariculture- MARIMIRNA, Rovinj). Comparison was madewith
fish from the wild population. Several
hematological(RBC,Hct,Hb,MCHC,MCV,MCH) and biochemical parameters
(totallipids, proteins, cholesterol, blood sugar, albumin and globulin)have
been measured. The obtained results indicated a significantseasonal
variability related to the environmental seasonalvariations and
particularly pertinent to the general metabolicactivity, sexual cycle and
other biological function.
Keywords: Sea bass, Dicentrarchus labrax, erythrocyte, hematocrit, hemoglobin, glucose, total plasma protein, total plasma lipids, cholesterol, electrophoresis.
- Type of paper
: Paper in journal
Title: Growth, chemical composition and hematological
characteristics of sea bass (Dicentrarchus labrax L.) grown on three
different diets
- Authors:
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
- Šterbić, Ivo
Journal: Veterinarski arhiv
Number: 1
Volume: 62
Year: 1992
Pages: from 51 to 61
Language: hrvatski
Summary: The growt rate, feed conversion, hematological parameters
andcomparative tissue composition were examined in three groups ofthe sea
bass fed on different diets. When experiment was started,17 month-old fish
had an average weight of 150-170g and thelenght of 23,5 - 24,6 cm. Two
commercial (A and B) and oneexperimental (C) dry pellet diet were tested.
Fish were sampledafter 73 and 130 days. The highest growth rate and final
bodyweight after 73 days were observed in the fish fed on diet A.After
prolonged feeding (130) days in all groups thegrowth rate indexes were
reduced but among them the highestgrowth rate was maintained in group B. In
general, a significantincrease of body fats was observed, and the highest
accumulationwas in the liver and abdominal cavity.
Keywords: Sea bass, Dicentrarchus labrax, feed conversion index, specific growth rate, commercial diet, hematological characteristics, proximate liver composition, proximate white muscle composition.
- Type of paper
: Paper in journal
Title:
- Authors:
- Ozretić, Bartolo (34426)
- Ozretić, Mirjana (34430)
Journal: Acta Adriatica
Number: 2
ISSN: 0001-5113
Volume: 32
Year: 1991
Pages: from 765 to 769
Language: engleski
Summary: In north Adriatic like in other seas, the concentration of
totalarsenic in foods of marine origin and in all marine animals
isgenerally exceeding the concentration approved by the sanitaryfood
quality regulation. However, from the dietary point, arsenicdoes not
represent a serious risk, because it is mainly presentin physiologically
and toxicology inert organic forms.
Keywords: Arsen, plankton, teleost fish, selachia, crustacea, mollusca,
- Type of paper
: Paper in journal
Title:
- Authors:
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
Journal: Pomorski zbornik
ISSN: 0554-6397
Volume: 29
Year: 1991
Pages: from 451 to 460
Language: engleski
Summary: Three years after sinking of the "Brigitta Montanari" it
wasnoticed that VCM started to leek from the wreck. This reportrepresents a
part of complex reserach program that was organizedas requested by
environmental protection authorities. VCM isknown as hepatotoxic and
teratogenic agent which inducesangiosarcoma in liver of experimental
animals and man. Thus ourinvestigations were focused to check the changes
in activity ofliver specific enzymes (GOT,GPT and SDH) in
representativebenthic fish caught by trawling nearby the wreckage.
Twocommeercially important fish species were analyzed:
europeanhake(Merluccius merluccius L.) and common pandora
(Pagelluserythrinus).
Keywords: Merluccius merlucius, Pagellus erythrinus, liver, vinyl chloride monomer, sorbitol dehydrogenase, glutamate pyruvate transaminase, glutamate oxalacetate transaminase
- Type of paper
: Paper in journal
Title:
- Authors:
- Ozretić, Mirjana (34430)
Journal: MAP technical reports series
Volume: 79
Year: 1994
Pages: from 1 to 15
Language: engleski
Summary: The present papers contribute to the identification
andcharacterization of mussel (Mytilus galloprovincialis) esterases,so that
these enzymes may be used as indicators of organophophateand carbamate
poisoning. Esterase were detected in almost allmussel tissues but the
highest activity was found inhepatopancreas. The existance of esterase
isozymes was identifiedby starch gel electrophoresis. Combining the
relative mobilitywith the substrate specificity and the sensitivity to
theinhibitors, esterase from mussle hepatopancreas were classifiedas
carboxylesterases and acetylsterases. In vitro studiesindicated that
organophoshate and carbamate pesticides havedifferent effects on different
esterase isozymes of mussel.
Keywords: Mussel, Mytilus galloprovincialis, hepatopancreas, esterase, carboxylesterase, acetylesterase, starch gel electrophoresis, esterase isozyme
- Type of paper
: Paper in journal
Title:
- Authors:
- Ozretić, Mirjana (34430)
Journal: MAP technical reports series
Volume: 69
Year: 1992
Pages: from 165 to 175
Language: engleski
Summary: This study was initiated to evaluate the changes in plasma
enzymeactivity in marine fish after acute exposure to pollutants. Adultgrey
mullets Mugil auratus were used as test organisms. Acuteliver injury was
experimentaly induced with high carbontetrachloride and phenol doses.
Lactate dehydrogenase (LDH),glutamate oxalacetate transaminase (GOT) and
glutamate pyruvatetransaminase (GPT) were selected as relevant enzymes
forevaluation of liver intoxication. Dose dependent increases of allthree
enzymes in plasma were observed promptly after injection ofthe toxicant.
The electrophoretic patterns of GOT from differentmullet tissues, and the
organ/tissue distribution of both GOT andGPT suggested liver to be their
origin.
Keywords: Grey mullet, liver, serum, lactate dehydrogenase, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, carbon tetrachloride, phenol
- Type of paper
: Paper in journal
Title:
- Authors:
- Žikić, Radoslav
- Ozretić, Mirjana (34430)
- Banković, Drago
Journal: Genetika
Number: 1
Volume: 26
Year: 1994
Pages: from 63 to 72
- Type of paper
: Paper in proceedings
Title:
- Authors:
- Ozretić, Mirjana (34430)
- Editors
- Gabrielides, Gabriel
Proceedings title: Proceedings of the FAO/UNEP/IOC workshop on the biological effects of pollutants
Language: engleski
Place: Athens, Greece
Year: 1992
Pages: from 165 to 175
Meeting: Biological effects of pollutants on marine organisms
Held: from 09/10/91 to 09/14/91
Summary: Besides glutamate oxaloacetate transaminase (GOT),
glutamatepyruvate transaminase (GPT) and lactic dehydrogenase (LDH) wehave
studied the possibility to use sorbitol dehydrogenase (SDH).glutamate
dehydrogenase (GLDH) and alkaline phosphatase (AP) toexamine liver
poisoning in grey mulet. Fish were treated withcarbon tetrachloride, phenol
and cyanide. The increased activityof GOT,GPT, SDH and GLDH and the changes
in concentration oftryglicerides in plasma can be used as positive
indicators ofliver damage by poisoning. The changes in activity of AP,
thedecreased concentration of plasma proteins, hemoglobin andhematocrit are
discussed.
Keywords: Grey mullet, liver, plasma enzyme, glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, sorbitoldehydrogenase, glutamate dehydrogenase, phenol, carbon tetrachloride, cyanide
Other: MAP Technical Reports Series No.69, Athens 1992
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Petrović, Siniša (124913)
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB 93
Language: engleski
Place: Zagreb
Year: 1993
Pages: from 80 to 80
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 06/17/93 to 06/18/93
Summary: Two aspartate aminotransferase isoenzymes were detected in
the
red muscle of grey mullet. One of them was purified to
homogeneity by chromatography on hydroxylapatite, ion exchange
columns, chromatofocusing and FPLC on the Mono Q column. The
isolated enzyme is a dimeric protein of identical subunits with
overall Mr about 65000, as judged by gel filtration and
polyacrylamide gel electrophoresis. It has a three
electrophoretically distinct subforms with isoelectric points of
8.50, 8.70 and 8.85. The Michaelis constants for L-aspartate,
2-oxoglutarate and oxaloacetate were estimated to be 0.30, 0.45
and 0.13 mM respectively, whereas Km for L-glutamate is 8.57 mM.
According to its subcellular distribution, molecular, catalytic
and inhibition properties isolated enzyme belongs to the
mitochondrial form of aspartate aminotransferase.
Keywords: Aspartate aminotransferase, Mitochondria, Grey mullet, Isolation, Characterization
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Petrović, Siniša (124913)
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
- Editors
- Decker, K.
Proceedings title: Book of Abstracts (22nd FEBS Meeting)
Language: engleski
Place: Stocholm, Sweden
Year: 1993
Pages: from 128
Meeting: 22nd Meeting of the Federation of European Biochemical Societies
Held: from 07/04/93 to 07/09/93
Summary: Two aspartate aminotransferase isoenzymes were detected in
thered muscle of marine fish, grey mullet. One of them was purifiedto
homogeneity by chromatography on hydroxylapatite, ion exchangecolumns,
chromatofocusing and FPLC on the Mono Q column. Theisolated enzyme is a
dimeric protein of identical subunits withoverall Mr= 65000, as judged by
gel filtration and polyacrylamidegel electrophoresis. It has a three
active, electrophoreticallydistinct, subforms with pI of 8.85, 8.70 and
8.50. Subcellularfractionation, molecular and catalytic properties,
together withinhibition kinetics, showed that the isolated enzyme belongs
tothe mitochondrial isoenzyme of aspartate aminotransferase.
Keywords: Aspartate aminotransferase, Mitochondria, Grey mullet, Purification, Characterization
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
- Najdek, Mirjana (83206)
Proceedings title: Bulletin Zoologishe museum, Special issue
Language: engleski
Place: Amsterdam, Netherlands
Year: 1991
Pages: from 82 to 0
Meeting: Seventh international ichthyology congress of the european ichtyological union
Held: from 08/26/91 to 08/30/91
Summary:
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
Proceedings title: Toxicity assessment and on-line monitoring
Language: engleski
Place: Berlin, Germany
Year: 1993
Pages: from 8 to 0
Meeting: Sixth international symposium Toxicity Assesment and On line Monitoring
Held: from 05/10/93 to 05/14/93
Summary: Toxicity of various polluting sdubstances was evaluated
using seaurchin gametes and their early developing stages.THe changedsperm
motility, fertilization, clevage and hatching rate, and thealtered DNA and
echinochrome production were parameters of mainconcerns. The results about
the evaluation of sperm motility bycentrifugation or by filtration of
diluted sperm were notconsistent with all used pollutants. However, the
best comparableresults were obtained by measurment of DNA and
echinochromeproduction rate. The percentage of unfertilized eggs,
thedecreased clevage rate and hatching time and the progressiveappearing of
various morphological deformations, observed bymicroscopic examinations are
the best actual evidence of thetoxic effects of the used substances.
Keywords: Sea urchin, gamete, blastulae, hatching, cleveage, sperm motility, DNA, echinochrome
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Gomerčić, Hrvoje
- Huber, Đuro
- Ozretić, Bartolo (34426)
- Škrtić, D.
Proceedings title: Veterinarska znanost i praksa
Place: Zagreb
Year: 1993
Pages: from 210 to 0
Meeting: Sixth international symposium Toxicity Assesment and On line Monitoring
Held: from 06/25/93 to 06/26/93
- Type of paper
: Summary in proceedings
Title:
- Authors:
- Petrović, Siniša (124913)
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
Proceedings title: Godišnji sastanak hrvatskih biokemičara HB 94
Language: engleski
Place: Opatija
Year: 1994
Pages: from 39 to 39
Meeting: Godišnji sastanak hrvatskih biokemičara
Held: from 10/14/94 to 10/15/94
- Type of paper
: Summary in proceedings
Title: Date shells : S.O.S. for the near shore rocky bottom
communities
- Authors:
- Ozretić, Bartolo (34426)
- Editors
- Šerman, Draško
Proceedings title: Zbornik priopćenja četvrtog kongresa biologa Hrvatske
Language: hrvatski
Place: Zagreb
Year: 1993
ISBN/ISSN: 953-6241-00-5
Pages: from 83 to 84
Meeting: Četvrti kongres biologa Hrvatske
- Type of paper
: Summary in proceedings
Title: Some histological and histochemical characteristics of one
species of bottlenose dolphin ( Tursiops sp.) from the Adriatic sea
- Authors:
- Gomerčić, Hrvoje
- Huber, Đuro
- Gomerčić, Vera
- Ozretić, Bartolo (34426)
- Vuković, S.
- Škrtić, D.
- Babac-Pašagić, G.
- Jukić-Brestovec, V.
- Editors
- Šerman, Draško
Proceedings title: Zbornik priopćenja četvrog kongresa biologa Hrvatske
Place: Zagreb
Year: 1993
ISBN/ISSN: 953-6241-00-5
Pages: from 84 to 85
- Type of paper
: Ph.D.
Title:
Faculty: Prirodoslovno-matematički fakultet Sveušilište u Zagrebu
Author: PETROVIĆ SINIŠA
Date of defense: 04/21/95
Language: hrvatski
Number of pages: 95
Summary: In this study the presence of cytosolic and mitochondrial
aspartate aminotransferase isoenzymes in the red muscle of grey mullet,
Mugil auratus Risso, was established. Aspartate aminotransferase isoenzymes
were isolated from the homogenate of the red muscle and characterized.
Separation of isoenzymes was achieved by hydroxylapatite column
chromatography. Cytosolic aspartate aminotransferase isoenzyme with
specific activity of 135 U/mg was prepared in electrophoretically
homogenous form. It was a dimeric protein composed of identical subunits
with overall Mr of about 65000. Isoelectric focusing revealed that
cytosolic isoenzyme was an acidic protein composed of several active
subforms. Mitochondrial aspartate aminotransferase isoenzyme was also
prepared in a homogeneous state. Isolated isoenzyme had relative molecular
weight of about 65000 and it consisted of two identical subunit like its
cytosolic counterpart. For mitochondrial isoenzyme, three active subforms
were identified by isoelectric focusing at pH 8.50, 8.70 and 8.85. The
purified isoenzyme was unstable to freezing and above 40řC. By measuring
its activity at different pH values, the enzyme stability was established
in the pH range from 7.0 to 10.5. Both isoenzymes of aspartate
aminotransferase obeyed Ping Pong Bi Bi kinetic mechanism of reaction.
Isolation and characterization of aspartate aminotransferase isoenzymes
from red muscle of grey mullet, demonstrated that it has the same
isoenzymic system as other vertebrate species thus far studied. According
to their catalytic properties and substrate affinities, aspartate
aminotransferase isoenzymes from red muscle may be involved in the
aspartate-malate shuttle as well as in the citric acid cycle.
- Type of paper
: Mentorship
Title:
Faculty: Prirodoslovno-matematički fakultet Sveušilište u Zagrebu
Mentor: OZRETIĆ MIRJANA
Date of defense: 04/21/95
Number of pages: 95
Author: Petrović Siniša
Degree level: Ph.D.
- Type of paper
: Survey/Study
Title:
- Authors:
- Ozretić, Bartolo (34426)
- Degobbis, Danilo
- Penzar, Branka
- Penzar, Ivan
- Zavodnik, Dušan
Ordering party: INGRA dd,
Institution depot: INGRA dd,
Year: 1992
Number of pages: 303
Language: talijanski
- Type of paper
: Survey/Study
Title:
- Authors:
- Ozretić, Bartolo (34426)
- Zavodnik, Dušan
- Degobbis, Danilo
- Penzar, Branka
- Penzar, Ivan
Ordering party: INGRA dd,
Institution depot: INGRA dd,
Year: 1993
Number of pages: 303
Language: hrvatski
- Type of paper
: Survey/Study
Title:
- Authors:
- Ozretić, Bartolo (34426)
- Kuzmanović, Niko
Ordering party: Urbis 72
Institution depot: Urbis 72
Year: 1994
Number of pages: 34
Language: hrvatski
- Type of paper
: Survey/Study
Title:
- Authors:
- Ozretić, Bartolo (34426)
- Degobbis, Danilo
- Zavodnik, Dušan
- Buttignoni, Vilma
- Martinčić, Darko
- Ozretić, Mirjana (34430)
- Brenko, Mirjana
Ordering party: Urbis 72
Institution depot: Urbis 72
Year: 1994
Number of pages: 46
Language: hrvatski
- Type of paper
: Radio broadcast
Title:
- Authors:
- Ozretić, Bartolo (34426)
Radio station: H.R. Pula
Name of emission (broadcast): Z kao zemlja
Year: 1994
Language: hrvatski
- Type of paper
: Radio broadcast
Title:
- Authors:
- Ozretić, Bartolo (34426)
Radio station: H.R. Pula
Name of emission (broadcast): Z kao zemlja
Year: 1994
Language: hrvatski
- Type of paper
: Radio broadcast
Title:
- Authors:
- Ozretić, Bartolo (34426)
Radio station: RAI
Name of emission (broadcast): Voci e volti dell Istria
Year: 1993
Language: talijanski
- Type of paper
: Radio broadcast
Title:
- Authors:
- Ozretić, Bartolo (34426)
Radio station: RAI
Name of emission (broadcast): Voci e volti dell Istria
Year: 1994
Language: talijanski
- Type of paper
: Manuscript
Title:
- Authors:
- Ozretić, Bartolo (34426)
- Petrović, Siniša (124913)
- Ozretić, Mirjana (34430)
- Type of paper
: Manuscript
Title:
- Authors:
- Ozretić, Mirjana (34430)
- Ozretić, Bartolo (34426)
- Petrović, Siniša (124913)
- Type of paper
: Manuscript
Title:
- Authors:
- Babac-Pašagić, G.
- Ozretić, Bartolo (34426)
- Type of paper
: Manuscript
Title:
- Authors:
- Petrović, Siniša (124913)
- Ozretić, Bartolo (34426)
- Ozretić, Mirjana (34430)
Language: engleski
Summary: Aspartate aminotransferase isoenzymes have a central role
in the amino acid metabolism and participate in maintaining redox balance
via an aspartate-malate shuttle in aerobic tissues such as red muscle
tissue. The aim of this study was to purify and characterize cytosolic
isoenzyme of aspartate aminotransferase from the red muscle of grey mullet.
Properties of the isolated isoenzyme will be compared with that of
cytosolic isoenzymes from other vertebrate species and its possible
physiological role will be discussed.
Keywords: Aspartate aminotransferase, Cytosol, Enzyme isolation, Enzyme characterization, Grey mullet
- Type of paper
: Invited lecture
Title:
Institution: Hrvatsko biokemijsko društvo
Year: 1994
- Type of paper
: Invited lecture
Title:
Institution: International Workshop- Trends in land use, water quality and fisheries: A comparison of the North Adriatic and Chesapeake bay
Year: 1995
- Type of paper
: Invited lecture
Title:
Institution: Berlin Universiry of Technology
Year: 1993
- Type of paper
: Other
Title:
