Biochemical indicators of toxicological stress in marine organisms
Main researcher
: OZRETIĆ, MIRJANA (34430) Assistants
PETROVIĆ, SINIŠA (124913)
OZRETIĆ, BARTOLO (34426)
Type of research: basic Duration from: 01/01/91. to 12/31/93. Papers on project (total): 38
Papers on project quoted in Current Contents: 4
Institution name: Institut "Ruđer Bošković", Zagreb (98) Department/Institute: Center for Marine research, Rovinj Address: Giordano Paliaga 5 City: 52210 - Rovinj, Hrvatska
Communication
Phone: 385 (0)52 811 544
Fax: 385 (0)52 813 496
Summary: Development of relatively fast and simple toxicity tests
and the characterization of appropriate marine test organisms to be used
for testing and biomonitoring the quality of the sea water environment was
the main concern of this project. In order to find most relevant
indicators for the early detection of the toxicological stress we
proceeded to evaluated qualitative and quantitative alterations of some
specific physiological and biochemical reactions in selected marine
organisms. Fish liver and the early embryo-developmetal phases of sea
urchin were adopted as model systems. Carbone tetrachloride, cyanides,
phenol and TBT were used as model toxicants. In fish, among several
plasma enzymes GPT, GLDH and SDH were adopted as specific enzymes,
indicative of cytotoxic lesion of liver, while the reactivity of LDH and
GOT as non specific liver enzymes were adopted to check any extra hepatic
harm as general indicators of stress. For that purpose GOT isoenzymes were
separated, purified, and characterized according to their molecular and
catalytic properties and to their sensitivity to inhibitors. In blood and
plasma of intoxicated fish, besides the enzyme activity we measured also
some hematological and biochemical parameters significant for the
estimation of the health condition of fish.The toxicity of TBT as
component of antifouling marine paints was evaluated on the basis of
several sea urchin embryotoxicity tests using their gametes and the early
embryonal stages, measuring the cleavage rate delay and the reduced
production of DNA and echinochrome.
Research goals: Marine toxicity tests are essential not only for the
assesment of specific effects of any substance introduced in the marine
environment, but also for the issue of emission standards and criteria
pertinent for evaluation and for the control of the marine environmental
quality. Most of these standards were up today estimated inadequately, on
the basis of ecotoxicological tests and surveys with fresh water
organisms. To find the most rapid, simple and accurate procedure for the
early warning of the ecological stress in marine environment, as well as
the introduction of sensitive and representative marine organisms to be
used for marine toxicity testing procedures were the main objectives of
this project. Grey mullet fish (Mugil auratus Risso) and sea urchin
(Paracentrotus lividus Lmk. and Sphaerechinus granularis L.) gametes and
their developing embryos were used. Specific organs, tissues, whole blood
and plasma in fish and early embryonal stages of sea urchin are likely to
fulfill most of the requirements for toxicity testing and have been
recomended for general use as marine test organisms.
COOPERATION - PROJECTS
Name of project
: YUG/100 - G Serum enzymes in fish as
biochemical incicators of marine pollution Name of institution: FAO/UNEP City: Athens, Greece
Name of project
: Yug/70-G Development of short-term toxicity
tests: The use of sea urchin gametes and their developmental stages Name of institution: FAO/UNEP City: Athens, Greece Other information about the project.