SVIBOR - Project code: 1-08-222

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

SVIBOR - Collecting Data on Projects in Croatia

Project code: 1-08-222


Mechanisms of DNA Damage and Repair in Marine Invertebrates


Main researcher: BATEL, RENATO (92412)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/93.
Papers on project (total): 32
Papers on project quoted in Current Contents: 8
Institution name: Institut "Ruđer Bošković", Zagreb (98)
Department/Institute: Center for Marine Research - Rovinj Division
Address: Giordano Paliaga 5
City: 52210 - Rovinj, Hrvatska
Communication
Phone: (385) 052 811 544
Fax: (385) 052 813 496
Summary: DNA damage in marine invertebrates caused by model pollution have been investigated. For this purpose methods for determination of single strand breaks, alkali-labile sites and crosslinks, without using ionizing radiation have been developed. Increased frequencies of strand breaks and DNA crosslinks in natural mussel populations under the influence of urban and industrial wastes, enable the classification of sampling sites of Rovinj area according to genotoxic impact on mussel DNA. The mechanism of DNA fragmentation in marine sponges involved in apoptosis, and modulation of this process by environmental pollutants, have been investigated, too. Expression of different genes involved in activation of pollutants, DNA damage and repair, has been analysed, including CYP 1A1, ubiquitin and heat-shock genes in fish, mussel and sponge. The results of our investigations gave additional informations about molecular mechanisms involved in activation and deactivation of contaminants, as well as DNA damage and repair in marine organisms.

Keywords: marine invertebrates, fish, DNA damage, alkaline-filter elution, cytochrome P-450 - cyp 1A1, ubiquitin, apoptosis, stress proteins, genotoxic pollutants

Research goals: The main goals of our investigations are understanding of mechanisms involved in activation and deactivation of pollutants in marine organisms, including induction of DNA damage and repair and gene expression involved in those proccesses. Development of methods for such measurements were neccessary. Results of these investigations should make possible estimation of presence of genotoxic pollutants in the sea as well as genotoxic risk assessment for marine organisms.

COOPERATION - PROJECTS


  1. Name of project: KRO-ENV 1 Effect of pollutants on apoptosis-programmed cell death (Molecular evaluation of pollution: Apoptosis)
    Name of institution: Abteilung fr Angewandte Molekularbiologie, Institut fr Physiologische Chemie, "Johannes Gutenberg" Universit„t, Mainz,
    City: 55099 - Mainz, Njemačka

COOPERATION - INSTITUTIONS


  1. Name of institution: Abteilung fr Angewandte Molekularbiologie, Institut fr Physiologische Chemie, "Johannes Gutenberg" Universit„t, Mainz
    Type of institution: University/Faculty
    Type of cooperation: Joint project
    City: 55099 - Mainz, Njemačka

  2. Name of institution: Arbeitskreis Molekulare Mechanismen Umgeweltbedingter Genotoxizit„t, Akademie der Wiesenschaften und der Literatur, Mainz
    Type of institution: University/Faculty
    Type of cooperation: Joint project
    City: 55099 - Mainz, Njemačka
Other information about the project.
MZT Croatian language SVIBOR Alphabetic list Sorted on project code Sorted on institutions Search help
Ministry of
Science and
Technology		 Croatian
language		 Svibor
homepage		 Alphabetic
list		 Sorted on
project code		 Sorted on
institutions		 Search Help
Last update: 09/28/95
Information: svibor@znanost.hr