SVIBOR - Project code: 1-08-276

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

SVIBOR - Collecting Data on Projects in Croatia

Project code: 1-08-276


INVESTIGATION OF SOME PLANT HORMONES IN PLANT DEVELOPMENT


Main researcher: REGULA, IVAN (40614)


Assistants
Type of research: basic
Duration from: 01/01/91. to 01/01/96.
Papers on project (total): 15
Institution name: Prirodoslovno-matematički fakultet, Prirodoslovni odjeli, Zagreb (119)
Department/Institute: Botany Department of Biology Faculty of Science
Address: Rooseveltov trg 6, HR-10000 Zagreb
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 412-912
Fax: 385 (0)1 4552-645
Summary: The presence of biogenic amines, histamine and particularly5-hydroxytriptamine - serotonin - in vegetative and generativeplant organs will be investigated. We shall try to identify thephase of ripening in which the biosynthesis of serotonin occurs.We shall try to determine the functions of biogenic amines intissues and particularly hormone activity of serotonin and itsmetabolite 5-hydroxyindole-3-acetic acid by biotests and tissueculture in vitro. The location of amine in tissue will bedetected by histochemical reactions. In these investigations weshall use chromatographic (thin layer, HPLC), electrophoretic,spectrophotometric, spectrophotofluorimetric andspectrocolorimetric methods.

Keywords: 5-hydroxytryptamine (serotonin), 5-hydroxyindole-3-acetic acid, Juglans cinerea, Juglans nigra, walnut, Carya, physiological activity

Research goals: 1. Investigation of the presence of serotonin, its metabolites and other biogenic amines (tryptamine, histamine) in plants, particularly in edible ones. 2. Detection of physiological function of serotonin and 5-hydroxyindole-3-acetic acid in differentiation and plant growth. 3. An attempt to inhibit serotonin transformation in 5-hydroxyindole-3-acetic acid by diamine oxidase activity. 4. Determination of phase development of fruit when adaptive enzyme formation occurs in serotonin synthesis. 5. Investigation of the presence of the above specified substances in plants which they have not been identified to date. Extraction will be carried out under cold conditions, concentration under mild conditions, separation of substances on ion exchange column, by paper and thin layer chromatography, electrophoresis, and detection by spectrocolorimetric, spectrofluorimetric and spectrophotometric methods. 6. Amine in tissues will be located by histochemical methods. 7. Detection of auxin activity of serotonin and 5-hydroxyindole-3-acetic acid will be determined by Avena coleoptile biotest, on root of barley, leaf discs of tobacco, by Allium test and tissue culture.
Other information about the project.
MZT Croatian language SVIBOR Alphabetic list Sorted on project code Sorted on institutions Search help
Ministry of
Science and
Technology		 Croatian
language		 Svibor
homepage		 Alphabetic
list		 Sorted on
project code		 Sorted on
institutions		 Search Help
Last update: 10/09/95
Information: svibor@znanost.hr