- Type of paper
: Paper in book
Title:
- Authors:
- Bašić, Ivan (2406)
- Grdina, D.J.
- Lyons, T.
- Editors
- Nygaard, Oddvar
- Upton, Arthur
Publisher: Plenum Press
ISBN: 0-306-44056-3
Year: 1991
Pages: from 297 to 301
Number of references: 7
Language: engleski
- Type of paper
: Paper in book
Title: Antimetastatic activity of bee venom and water - soluble
derivative of propolis in mice
- Authors:
- Bašić, Ivan (2406)
- Ćurić, Stipica (108013)
- Tadić, Zoran (143012)
- Oršolić, Nada
- Sulimanović, Đuro (45465)
- Editors
- Cochard, Jean - Paul
Publisher: International Apicultiral Organization
Year: 1996
Number of references: 11
Language: engleski
- Type of paper
: Paper in journal
Title:
- Authors:
- Kataoka, Y.
- Bašić, Ivan (2406)
- Perrin, J.
- Grdina, D.J.
Journal: International Journal of Radiation Biology
Number: 3
ISSN: 0020-7616
Volume: 61
Year: 1992
Pages: from 387 to 392
Number of references: 27
Language: engleski
Summary: The antimutagenic effects of the radiation protective agent
S - 2- (3 - aminopropylamino)ethylphosphorothionic acid (WR - 2721)were
studied against fission - spectrum - netron and 60 Co gammaray - induced
mutagenesis in mice. Mutagenesis at the hypoxantine- guanine phosphoribosyl
transferase (HPRT) locus was measured 56days following whole - body
irradiation with JANUS neutrons(single doses, 50 - 150 cGy) or 60 Co
photons (single doses, 250- 750 cGy). Splenic T lymphocytes from B6CF1 mice
were grown inround - bottomed 96 - microwell culture plates with or
withoutthe selective agent 6 - thioguanine (6 - TG). Mutant frequency,as a
result of exposure to neutrons or 60 Co photons, increased100-fold with
dose. Doses of 150 cGy neutrons and 750 cGy 60 Cophotons were equally
mutagenic. When animals were injected withWR - 2721 at a dose of 400 mg/kg
body weight, i.p., 30 min beforewhole-body irradiation with JANUS neutrons
or 60 Co photons,mutant frequencies were significantly reduced at all
radiationdoses (i.e. protection factors of 1.4 and 2.4, respectively.Thus,
the aminothiols are effective antimutagens. a novelclinical application of
these compounds could be in their use toprotect against radiation - and/or
chemotherapy induced genotoxicdamage to normal cells.
- Type of paper
: Paper in journal
Title:
- Authors:
- Ćurić, Stipica (108013)
- Oršolić, Nada
- Krsnik, Boris (23981)
- Balenović, Tomislav (1771)
- Valpotić, Ivica (66266)
- Sulimanović, Đuro (45465)
- Bašić, Ivan (2406)
Journal: Stočarstvo
Number: 3
ISSN: 638 -1394
Volume: 47
Year: 1993
Pages: from 131 to 136
Number of references: 11
Language: engleski
Summary: The effect of honeybee venom on humoral (gamma globulin and
totalprotein levels) and cellular immune responses (weight andcellularity
of spleen and lymph nodes) was tested in CBA mice.Bee venom was injected
subcutaneously into the left footpad at adose of 0.15, 0.30 or 0.60 mg per
mouse, respectively. Mean gammaglobulins and total protein values were
significantly elevated (P< 0.05) in mice treated with 0.15 mg or 0.30 mg of
bee venom,respectively, compared to those recorded in nontreated
controls.Weight of the left popliteal lymph node (LPLN) was
significantlyhigher (P < 0.01) in mice given 0.30 mg or 0.60 mg of bee
venomthan that of the controls. the cellularity of LPLN in bee venom
-treated mice was much greater (P < 0.05; < 0.01) when compared tothe
control values. However, no differences in weight andcellularity of the
popliteal lymph node were noticed in micetreated with identically with
propolis, used as an antigen. Therewere no differences in weight or
cellularity of spleen betweentreated and control mice.
- Type of paper
: Paper in journal
Title:
- Authors:
- Šamija, Mirko (66200)
- Eljuga, Damir (87925)
- Košuta-Špoljar, Dunja (81851)
- Gerenčer, Marijan
- Bašić, Ivan (2406)
Journal: Libri Oncologici
Number: 3
ISSN: 0300-8142
Volume: 20
Year: 1991
Pages: from 131 to 133
Number of references: 13
Language: engleski
Summary: The effect of primary local irradiation and IL - 2
preparationadministration on the number of spontaneous metastases to
thelungs of Y-59 rats was studied. Spontaneously formedtransplantable
anaplastic carcinoma (ACA) in Y-59 rats was usedin the experiment. On the
6th day following inoculation of 5 x 105 ACA cells into the foot of a
posterior leg, the entire footpadwith developing tumor was irradiated a
single dose of 20 Gy quickelectrons. Then, a group of animals was
intraperitoneallyinjected 500 IU IL - 2 preparation each 12 hours through 3
days.The animals were sacrificed on the 22nd day when the number
ofmetastases to the lungs was determined. Observed was that thenumber of
spontaneous metastases was considerably smaller inlocally irradiated and
intraperitoneally IL - 2 preparationinjected animals.
Keywords: primary tumor, interleukin 2, antimetastatic effect
- Type of paper
: Paper in journal
Title:
- Authors:
- Grdina, D.J.
- Kataoka, Y.
- Bašić, Ivan (2406)
- Perrin, J.
Journal: Carcinogenesis
Number: 5
ISSN: 0143-3334
Volume: 13
Year: 1992
Pages: from 811 to 814
Number of references: 32
Language: engleski
Summary: An "in vitro" T lymphocyte cloning technique has been
applied tostudy the effects of JANUS fission - spectrum neutron
irradiationand radioprotector S - 2 - (3
-aminopropylamino)ethylphosphorothioic acid (WR - 2721) on thesubsequent
development of somatic mutations at the hypoxantine -guanine phopshoribosyl
transferase (hprt) locus in hybrid B6CF1male mice. In control studies
performed to establish an "invitro" cloning technique, the mutant
frequencies of splenic Tlymphocytes, as result of exposure to a 100 cGy
dose of neutrons,increased with time from a control level of 9 X 10 -7 to
maximumvalue of 1.7 X 10 -5 at 56 days following irradiation between 56and
150 days after irradiation mutant frequencies were observedto plateau and
remain stable. all suqsequent determinations wereperformed at 56 days
following the experimental treatment ofanimals. WR - 2721 at a dose of 400
mg/kg was effective inprotecting against the induction of HPRT mutants
(i.e. mutantfrequency reduction factor, MFRF)following the largest dose
ofneutrons used (i.e. 150 cGy), whether it was administered i.p. 30before,
5 minutes after, 3 hours after or 3 times at 3, 24 and 48hours after, as
evidenced by MFRFs of 6.0, 6.6, 4.8 and 5.8respectively. Antimutagenic
effectiveness of WR - 2721administered 30 minutes prior to irradiation was
unaffected, evenwhen the dose was reduced to 200 mg/kg, MFRF = 7.0; 100
mg/kg,MFRF = 3.8; and 50 mg/kg, MFRF = 8.9. These findings confirm
ourearlier report using the radioprotector N - (2 - mercaptoethyl) -1,3 -
diaminopropane (WR - 1065) under "in vitro" conditions, anddemnonstrate
that these agents can be used as effectiveantimutagens even when they are
administered up to 3 hoursfollowing radiation exposure.
- Type of paper
: Paper in journal
Title:
- Authors:
- Tadić, Zoran (143012)
Journal: Libri Oncologici
Number: 3
ISSN: 0300-8142
Volume: 21
Year: 1992
Pages: from 153 to 159
Number of references: 13
Language: engleski
Summary: Hepatitis B virus is etiologic agent of acute hepatitis and
isconsidered to be a major factor in hepatocellular carcinomainitiation.
Here, the tranforming capability of normal andmutated HBV X gene was
investigated. NIH 3T3 cells weretransfected with plasmids carrying normal X
gene and frameshiftmutant in which only small part is translated correctly.
Bothconstructs were driven by Rous sarcoma virus LTR promoter.
Thetransformed phenotype was tested in focus - forming assay andability to
grow in semisolid medium (soft agar). In both testmutated HBV X gene showed
significantly greater transformingcapability than its normal counterpart.
The possible mechanismsof these observation are discussed with the special
emphasis onadditional open reading frames within the X ORF.
Keywords: hepatitis B virus, gene X, transformation
- Type of paper
: Paper in journal
Title:
- Authors:
- Ćurić, Stipica (108013)
- Tadić, Zoran (143012)
- Valpotić, Ivica (66266)
- Sulimanović, Đuro (45465)
- Bašić, Ivan (2406)
Journal: Veterinarski Arhiv
ISSN: 618 -006
Volume: 62
Year: 1992
Pages: from 31 to 35
Number of references: 10
Language: engleski
Summary: The effect of honeybee venom on the tumor growth and
metastasisformation in mice was studied. Bee venom was injected into
miceeither subcutaneously (s.c.) or intravenously (i.v.) at differentdoses.
The tumor was transplantable mammary carcinoma (MCA)weakly immunogenic to
the syngeneic CBA mouse. The tumor wasgenerated by injecting 10 5 MCA cells
i.v.. When the tumor cellswere injected s.c. into the footpad immediately
after bee venom,the growth of the tumor was supressed regardless of dose of
thevenom. Survival of the mice treated with 0.30 mg of bee venom
wasprolonged as compared to the controls. the number of lungmetastases in
the mice treated i.v. with 0.15 or 0.075 mg of beevenom was significantly
lower (P < 0.001) than that in nontreated mice. However, both doses of bee
venom given s.c. did notreduce the number of lung metastases indicating
that theantitumor effect of the venom could be highly dependent on theroute
of injection.
Keywords: murine mammary carcinoma, antitumor and antimetastatic activity, honeybee venom
- Type of paper
: Paper in journal
Title:
- Authors:
- Ćurić, Stipica (108013)
- Valpotić, Ivica (66266)
- Sulimanović, Đuro (45465)
- Bašić, Ivan (2406)
Journal: Veterinarski Arhiv
ISSN: 618 -006
Volume: 62
Year: 1992
Pages: from 43 to 47
Number of references: 7
Language: engleski
Summary: The effect of honeybee venom on humoral (gamma globulin and
totalprotein levels) and cellular immune responses (weight andcellularity
of spleen and lymph nodes) was tested in CBA mice.Bee venom was injected
subcutaneously into the left footpad at adose of 0.15, 0.30 or 0.60 mg per
mouse, respectively. Mean gammaglobulins and total protein values were
significantly elevated (P< 0.05) in mice treated with 0.15 mg or 0.30 mg of
bee venom,respectively, compared to those recorded in nontreated
controls.Weight of the left popliteal lymph node (LPLN) was
significantlyhigher (P < 0.01) in mice given 0.30 mg or 0.60 mg of bee
venomthan that of the controls. the cellularity of LPLN in bee venom
-treated mice was much greater (P < 0.05; < 0.01) when compared tothe
control values. However, no differences in weight andcellularity of the
popliteal lymph node were noticed in micetreated with identically with
propolis, used as an antigen. Therewere no differences in weight or
cellularity of spleen betweentreated and control mice.
Keywords: honeybee venom, immune response, CBA mice
- Type of paper
: Paper in journal
Title:
- Authors:
- Tadić, Zoran (143012)
- Bašić, Ivan (2406)
Journal: Croatian Journal of Gastroenterology and Hepatology
Number: 4
ISSN: 0353-9296
Volume: 1
Year: 1992
Pages: from 175 to 178
Number of references: 10
Language: engleski
Summary: Using retroviral vector, an efficient expression of the HBV
Xgene was achieved. Molecular analysis of isolated cellular DNAshowed
integrity of the provirus and Northern hybridizationconfirmed functionality
of the integrated provirus. The tests oftransformed phenotype revealed the
mutated HBV X gene totransform NIH 3T3 cells more efficiently that the wild
type gene.
Keywords: hepatitis B virus, DNA viral, genetic vectors, retroviridae
- Type of paper
: Paper in journal
Title:
- Authors:
- Eljuga, Ljerka
- Šamija, Mirko (66200)
- Eljuga, Damir (87925)
- Košuta-Špoljar, Dunja (81851)
- Bašić, Ivan (2406)
Journal: Libri Oncologici
Number: 3
ISSN: 0300-8142
Volume: 22
Year: 1993
Pages: from 177 to 181
Number of references: 15
Language: engleski
Summary: The effect of lymphokine - activated killer (LAK) cells
andrecombinant interleukin - 2 (rIL - 2) on the development ofpulmonary
metastases of anaplastic cxarcinoma (ACA) in Y59 ratand on the survival of
animals was studied. LAK cells (10 7) weregiven intravenously (i.v.) 4 days
after i.v. injction of 3 X 10 3tumor cells.Immediately after the
administration of LAK cellsanimals received i.p. an injection containing
100 IU of rIL - 2.Injections of rIL - 2 were done given for 3 consecutive
days in 8hour intervals. The combined use of LAK cells and rIL - 2produced
a pronounced antimetastatic activity and significantlyextended the survival
of animals.
Keywords: metastasis, rIL - 2, LAK cells
- Type of paper
: Paper in journal
Title: Developing concept of macrophage activation
- Authors:
- Verstovšek, Srđan
- Bašić, Ivan (2406)
Journal: Libri Oncologici
Number: 1
ISSN: 0300-8142
Volume: 24
Year: 1995
Pages: from 9 to 17
Number of references: 54
Language: engleski
Summary: The functional potential of macrophages is one of the major
criteria used to differentiate morphologically mature tissue macrophages.
However, the spectrum of macrophage functional potential is broad and it
can be expressed in a number of different ways upon exposure to one or more
different stimuli in the tissue environmrent. It is discussed that the
concept of activation in reality encompasses multiple states of macrophage
functional potential, multiple activation pathways and multiple stages of
activation. Thus despite the fact that mononuclear phagocytes belong to a
single system, it is suggested that they display varied functional
properties based on the local environmental pressures under which they
develop, including regulation by cells of other systems, and, further that
their responsiveness to extracellular signals is dependent upon their
developmental stage in the activation process at the time the signal is
applied.
Keywords: macrophages, heterogeneity, activation
- Type of paper
: Summary in proceedings
Title: Effect of local administration of cytostatic and
biological response modifiers (BRM) on liver metastasis of a colon tumor in
the rat
- Authors:
- Oršolić, Nada
- Kujundžić, Milan (168490)
- Kaštelan, Maja
- Tadić, Zoran (143012)
- Ćurić, Stipica (108013)
- Bašić, Ivan (2406)
- Editors
- Gomerčić, Hrvoje
Proceedings title: Zbornik sažetaka priopćenja petog kongresa biologa Hrvatske s međunarodnim sudje
Language: hrvatski
Place: Zagreb
Year: 1994
ISBN/ISSN: 953-6241-01-3
Pages: from 162 to 163
Meeting: Peti kongres biologa Hrvatske s međunarodnim sudjelovanjem
Held: from 10/03/94 to 10/07/94
Summary: Using a model of artificial liver metastasis of a weakly
immunogeneic colon adenocarcinoma of the rat, the antitumor effect of
intrahepatally administered high doses of doxorubicin or 5-FU combined with
intrasplenic ( IS ) administration of biological rensponse modifiers (
levamisole, IL-2, or a lyophilysed extract of the plant Caucalis
platicarpos L.) was studied.Animal that received cytostatics aaalone
survived significantly longer than untreated control; the increase of the
dose resulted in better survival and the lesions to bone marrow in these
animals were not augmented.Complete disappearance of liver metastasis was
frequently observed when administration of cytostatics combined with either
of BRM.In addition, BRM combined with cytostatics prevented the
dissemination of tumor cells to other organs.Immunological parameters such
as NK activity and response to mitogens in animal receiving combbined
therapy were higher than those in respective controls.In conclusion,the
results showed that appropriate administration of cytostatics and BRM may
be beneficial in the treatment of liver metastasis. It is likely that
splenic macrophages played a mayor role in controlling of tumor growth in
the liver.
- Type of paper
: Summary in proceedings
Title: The effect of hyperbaric oxygen on the growth of the
anaplastic carcinoma in Y59 rats lungs - cellular and humoral response
- Authors:
- Košuta-Špoljar, Dunja (81851)
- Arbanasić, Haidi
- Bašić, Ivan (2406)
- Editors
- Gomerčić, Hrvoje
Proceedings title: Zbornik sažetaka priopćenja petog kongresa biologa Hrvatske s međunarodnim sudje
Place: Zagreb
Year: 1994
ISBN/ISSN: 953-6241-01-3
Pages: from 163 to 164
Meeting: Peti kongres biologa Hrvatske s međunarodnim sudjelovanjem
Held: from 10/03/94 to 10/07/94
Summary: The effect of hyperbaric oxygen ( HBO ) on the growth of
anaplastic carcinoma ( ACa ) colonies of lungs of Y59 rats observed. From
the first day after i.v. tumor cell injection ( 2x104 ACa cells ) the
animals were exsposed to hyperbaric oxygen ( 300 kPa ) during 18 days ( 90
min/day ). The number of colonies on the lung;s surface of the rats
exsposed to HBOwas significantly smaller in number ( 4,2+-0,9 ) in
comparison to control animals ( 44,3+-8,2 ). We have also found the weight
of lungs to be significantly smaller ( 1,5+-0,09 g )in comparison with the
control values ( 4,21+-0,05 g ). There has been found that 65% of rats
exsposed to HBO didn;t have any macro- or micrometastases in their lungs.
Mononuclear cells from experimental and control animals were purified on
Ficoll Isopaque density gradient.Peripheral blood lymphocytes ( PBL ) (
2x105 ) and splenocytes were stimulated with policlonal mitogens
Phytohemagglutinin ( PHA ), Concanavalin A ( Con A ) and lypopolysacharide
( LPS ). Proliferation of PBL andsplenocytes was enhanced in those animals
treated after ACa with HBO and showed significantly higher values in
comparison to control animals ( given ACa cells ). However, reaction of HBO
treated animals to mitogens was similar to normal healthy rats. Humoral
immunological response was measured by plaque forming cells ( PFC ).
Results showed that there is no significantly difference between
experimental and control animals in different time intervals, except at day
20 after application of ACa cells in animals treated with HBO. These
findings suggest that HBO may promote a new population of precursor cells
to mitogenic responsive state and positive effect of HBO.
- Type of paper
: Summary in proceedings
Title: Royal jelly immunomodulation in rats and mice
- Authors:
- Šver, Lidija
- Oršolić, Nada
- Tadić, Zoran (143012)
- Valpotić, Ivica (66266)
- Bašić, Ivan (2406)
- Editors
- Gomerčić, Hrvoje
Proceedings title: Zbornik sažetaka priopćenja petog kongresa biologa Hrvatske s međunarodnim sudje
Place: Zagreb
Year: 1994
ISBN/ISSN: 953-6241-01-3
Pages: from 212 to 213
Meeting: Peti kongres biologa Hrvatske s međunarodnim sudjelovanjem
Held: from 10/03/94 to 10/07/94
Summary: In order to study possible immunomodulatory effect of royal
jelly ( RJ ) secreted by mandibular and hypopharyngeal glands of worker
honeybee ( Apis mellifera L. ) we have used well established rodent model.
CBA mice received s.c. 0,1 ml of RJ ( Experiment 1 ), and Y59 rats were
given i.m. 0,4 ml or i.v. 0,025 ml of RJ, respectively ( Experiment 2 ), by
one or two injection in 7 day intervals. In mice which were immunized with
4x108 of SRBC 7 days after application of RJ the number of plaque forming
splenocytes was significantly higher ( P<0,05 ) than that in nontreated
controls. Both, weight inguinal lymph node and number of peripheral blood
lymphocytes were increased ( P<0,05 ) in RJ- treated mice 3 or 5 days after
immunization, respectively. Neutrophils were decreased ( P<0,05 ) in the
mice that were killed 5 or 10 days after RJ treatment. Serum levels of
total proteins and immunoglobulins in rats that received RJ two times
within 2-week-period were significantly lowered ( P<0,05 ) as compared with
nontreated animals. Observed immunomodulatory effect of RJ should be
additionally tested in vitro using immunocompetent cells and purified
active substance(s) isolated from RJ.
- Type of paper
: Summary in proceedings
Title: Antimetastatic activity of bee venom and water - soluble
derivative of propolis in mice
- Authors:
- Bašić, Ivan (2406)
- Ćurić, Stipica (108013)
- Tadić, Zoran (143012)
- Oršolić, Nada
- Sulimanović, Đuro (45465)
- Editors
- Borneck, P.
- Stern, W.
- Cochard, Jean - Paul
Proceedings title: 34th International Apicultural Congress - Programme and summaries of the reports
Language: engleski
Place: Bucharest, Romania
Year: 1995
Pages: from 135 to 136
Meeting: 34th Congress Apimondia
Held: from 08/15/95 to 08/19/95
Summary: The effect of honeybee venom and water soluble propolis
derivates ( WSD ) on tumor growth and metastasis formation in CBA mice was
studied. Bee venom was injected into mice SC at doses of 0,15, 0,30, 0,60
mg/mouse and 0,15 or 0,075 mg/mouse SC or IV, respectively.WSD comprising
0,50 mg/kg of propolis was given IV. The tumor was a transpateble mammary
carcinoma ( MCA ) weakly immunogenic to syngeneic CBA mice. Metastases were
generated by injecting 1x103 MCA cells IV. The tumor in footpad was
generated by 1x105 tumor cells.
When tumor cells were injected into the footpad immediately after the bee
venom, the growth of tumor was suppressed regardless of the dose of the
venom. The survival of mice treated with 0,30 mg of bee venom was prolonged
as compared to that of the control. Number of lung metastases in mice
treated IV with 0,15 or 0,075 mg of bee venom was significantly lower (
P<0,001 ) comparing to that in nontreated mice. However, both doses of bee
venom given SC did not reduce a number of lung metastases, indicanting that
the antitumor effect of the venom couldbe highly depedent on the route of
injection. WSD of propolis given to mice 7 days before IV administration
of tumor cells significantly reduced the number of lung metastases.
However, an injection of WSD o0f propolis given at the time of tumor cells
injection or 7 days after had no effect on the number of metastases in the
lung. The changes in several immunological parameters such as the response
of lymphocytes to policlonal mitogens in vitro, production of tumor
necrosis factors ( TNF- ) by lymphoid cells in vitro and rosette
formation of lymphoid cells with SRBC, respectively corresponded positively
with antimetastatic properties of both bee venom and WSD of propolis.
Other: Poster PS 246 presented on 34th Congress of APIMONDIA:
Z. Tadić, N. Oršolić, V. Lacković, S. Ćurić, I. Bašić: The effect of
propolis on haematopoiesis (p. 64. in 34th Congress Apimondia Programme and
summaries of the reports)
- Type of paper
: Summary in proceedings
Title: Atimetastatic efficacy of levamisole and 5 - FU as
adjuvant to surgery of a rat colonic carcinoma
- Authors:
- Bašić, Ivan (2406)
- Kaštelan, Željko
- Eljuga, Damir (87925)
- Kaštelan, Maja
- Oršolić, Nada
- Tadić, Zoran (143012)
Proceedings title: 16th International Cancer Congress 1994 - Abstract Book - I (Oral & Poster Prese
Language: engleski
Place: New Delhi, India
Year: 1994
Pages: from 305 to 305
Meeting: 16th International Cancer Congress 1994
Held: from 10/30/94 to 11/05/94
Summary: The result of surgical removal of a large bowel tumor is of
uncertain prognosis for a patient because of possible previous release of
tumor cells from primary tumor into systemic and portal circulation. We
have studied the effect of surgery combined with adjuvant therapy with
levamisole and 5-FU on the development of liver metastases of a
transpantable rat colonic carcinoma and the effect of such therapy on
immunological status of rats, respectively. Tumor was generated by
injection 5x103 tumor cells into the colon wall. Twenty one days after
inoculation of tumor cells, the tumor was surgically removed and animals
wetre allocated to the following regimes: (a) no treatment, (b) levamisole
( 4 mg/kg every 8 h during three postoperative days and then on days 14 and
21 ), (c) 5 -FU ( 10 mg/kg, same schedule as for levamisole ), and (d) the
combination of levamisole and 5-FU at the same dose and schedule as above.
Animals were checked for liver metastases 51 days after the initiation of
the experiments. Significantly fewer adjuvant-treated animals had
metastases than those undergoing surgery alone ( P<0,05-0,001 ). Combined
treatment with both 5-FU and levamisole after surgery restored
immunological reactivity of spleen cells ( NK, proportion of T and B
lymphocytes, actyvity to mitogens ) which was deteriorated either by the
presence of the tumor or by surgery and 5-FU treatment, respectively. In
conclusion, adjuvant terapy with levamisole plus 5-FU suppressed the
growth of metastases of colonic adenocarcinoma in the liver partially
through the reparation of immune reaction.
Keywords: colon, tumor metastasis, levamisole, 5 - FU
Other: Sažetak se nalazi u knjizi pod brojem PSB12 - 07
- Type of paper
: Summary in proceedings
Title: Effect of locally administered cytostatic therapy and BRM
on liver metastasis of a colon tumor in the rat
- Authors:
- Bašić, Ivan (2406)
- Kujundžić, Milan (168490)
- Kaštelan, Maja
- Oršolić, Nada
- Tadić, Zoran (143012)
- Ćurić, Stipica (108013)
- Šamija, Mirko (66200)
Proceedings title: 16th International Cancer Congress 1994 - Abstract Book I - Oral & Poster Presen
Language: engleski
Place: New Delhi, India
Year: 1994
Pages: from 339 to 339
Meeting: 16th International Cancer Congress 1994
Held: from 10/30/94 to 11/05/94
Summary: Using a model of artifical liver metastasis of a weakly
immunogeneic colon adenocarcinoma of the rat, the antitumor effect of
intrahepatally administered doxorubicin of 5 - FU combined with ip
administration of the biological response modifiers (BRM), levamisole, IL -
2 or a lyophilized extracct of the plant Caucalis platycarpos L. was
studied. Animals that received cytostatics alone survived significantly
longer than untreated control; the increase of the dose resulted in better
survival and the lesions to the bone marrow in these animals were not
augumented. Complete disappearance of liver metastasis was frequently
observed when the administration of cytotoxic drug was combined with either
biological response modifier. In addition BR added to the drug therapy
prevented the dissemination of tumor cells to the other organs.
Immunological parameters such as NK activity and response to mitogens in
animals treated with cytotoxic drugs and RM were elevated In conclusion,
the results showed that appropriate administration of cytostatics together
with BRM may be beneficial in the treatment of liver metastasis.
Keywords: colon tumor, BRM, combined therapy
Other: Sažetak je naveden u knjizi pod brojem PSB23 - 13
- Type of paper
: Ph.D.
Title: The effect of chemotherapy and immunomodulators on liver
metastases of the colon adenocarcinoma in rats
Faculty: Medicinski fakultet Sveučilište u Zagrebu
Author: AGANOVIĆ IZET
Language: hrvatski
Number of pages: 127
Summary: This study was conducted to investigate the effect of
combinedchemoimmunotherapy on the colorectal carcinoma and livermetastases
in rats. It has been demonstrated that intrahepaticadministration of
daunorubicin and lipiodol as well asdoxorubicin, 5 - fluorouracil in
combination with lipiodolresulted in significant improvement in the rate of
survival amongexperimental animals and complete disappearance of liver
tumors.The results of treatment without lipiodol administration were,
tosome extent, inferior. Administration of 5 - fluorouracil alonedid not
produce satisfactory results. the results of treatmentwith the above
mentioned combined treatment modalities weresignificantly better if various
immunomodulatory agents have beenadministered simultaneously. Namely,
levamisole producedsignificant antitumor effect, as previously mentioned in
theliterature. however, the new immunomodulator, plant extract namedCDL
produced better results than levamisole. By using test oflymphocyte blast
transformation by mitogens and NK activity assaywe showed that levamisole,
IL - 2 and CDL increased T lymphocyteand NK cell activity. On the other
hand, no detrimental effectson liver functions, hematological parameters
and bone marrowcellularity were noted. In summary, we concluded that the
abovementioned forms of chemoimmunotherapy did not produce seriousadverse
effects, deserving great attention in furtherinvestigations and possible
clinical applications.
- Type of paper
: Ph.D.
Title: The transforming and transactivating capability of the
hepatitis b virus X gene
Faculty: Prehrambeno - biotehnološki fakultet Sveučilište u Zagrebu
Date of defense: 03/17/92
Language: hrvatski
Number of pages: 140
Summary: In this series of experiments, the transforming
andtransactivating potential of normal and mutated hepatitis B virusX gene
was investigated. Transfection of Hep G2 cells with normaland mutated HBV
genomes showed that gene X is not involved inviral replication but is
responsible for DNA packaging andformation of viral particles. The
transforming potential of the Xgenes was investigated using focus - forming
assay on confluentmonolayers of NIH 3T3 cells and colony formation of NIH
3T3fibroblasts and BNL CV 2 cells in soft agar. In this assay, themutated
HBV X gene showed greater transforming potential than itsnormal
counterpart. Also, NIH 3T3 cells with mutated X geneformed more tumors in
nude mice. The transactivating ability ofthe HBV X gene was assayed on the
SV 40 early promoter. It wasshown that normal gene is potent transactivator
while mutatedgene failed to activate transcription "in trans".
Thetransactivating ability of normal HBV X gene is preserved inintegrated
state in HuH - 7 cells. However, CAT assays withdifferent parts of HuH - 7
integrate confirmed the existence ofanother transactivator, presumably in
the S region of the HBVgenome. The expression of the HBV X gene in
retroviral vector(proviral expression) confirmed the results obtained
usingplasmids the HBV X genes. On the basis of this experiments, anew, dual
function of ORF X can be postulated. The first productof HBV X gene is
strong transactivator but cannot transform cells"in vitro". The second
product does not have transactivatingability but is capable of transforming
NIH 3T3 and BNL CV 2cells.
- Type of paper
: Mentorship
Title: Induction of murine splenic tumoricidal macrophages by
cytokines
Faculty: Prirodoslovno - matematički fakultet Sveučilište u Zagrebu
Mentor: BAŠIĆ IVAN
Number of pages: 204
Author: Verstovšek Dr. Srđan
Degree level: Ph.D.
