Type of research: basic Duration from: 01/01/92. to 12/31/94. Papers on project (total): 0
Institution name: Institut "Ruđer Bošković", Zagreb (98) Department/Institute: Department of Molecular Medicine Address: Bijenička cesta 54 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 42 42 39
Summary: This project will be discuss a fate and a role of
transferrinereceptor in the course of differentiation and maturation
ofchicken erythroblast cell line (HD3 cell line). After inductionof
differentiation, the level of transferin receptor increases inthe cells.
Parallel with that event, transferrin receptor isreleasing from the cells
in the exosome (vesicle). That phenomenis charasteristic for
differentiation and maturation of mammalianred cells. The explanation of
this phenomen will be help inunderstanding the mechanism of differentiation
and maturation ofred blood cells. At the same time, the relation
betweentransferrin receptor and hemoglobin synthesis will be discuss.With
different experiments might be possible to determine theirsconection. Does
synthesis of Hb depend on transferrin receptorsynthesis, or which of them
is primary. The special attentionwill be give to the process responsible
for the formation andrelease of exosomes. From that results we could see
whether theexosome formation and its releasing is characteristic for
alldifferentiating cells, or it is only a way for getting rid ofsome plasma
embrane proteins.
Research goals: The aim of this study was to elucidate the process
of avian red cell development. During the maturation process of red cells
many plasma membrane functions are diminish, nucleus and some internal
structures (ribosomes and mitochondria) are extruded. From many studies was
concluded that exosome formation and release is one route for remodelling
the plasma membrane of imature red cells and a mechanism for the loss of
specific membrane functions during maturation to the erythrocyte stage.
With different approach I tryd to answer on some questions. Do chicken red
cells share the property of exosome formation during maturation with
mammalian cells because they retain nucleus and mitochondria? At which
stage of red cell development is initiated the exosome formation? Does the
process occur only at a late stage of development? In this route of ridding
the cell of functions that have become obsolete or physiologically
redundant associated only with penultimate stage of red cell development
(i.e., reticulocyte stage)? Or, alternatively, does exosome formation occur
as part of commitment to the red cell lineage and continue on to the final
pre-erythrocyte stage? Second approach was to find the relationship between
hemoglobin formation, increased transferrin receptor expression, and the
appearance of exosomes bearing transferrin receptor.
COOPERATION - PROJECTS
COOPERATION - INSTITUTIONS
Name of institution
: Department of Biochemistry, Department of
Neurochemistry, McGill University Type of institution: Economical/Production City: Montreal, Kanada Other information about the project.