SVIBOR - Project code: 1-08-307

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 1-08-307


DIFFERENTIATION OF AVIAN RED BLOOD CELLS


Main researcher: GRDIŠA, MIRA (73264)



Assistants
Type of research: basic
Duration from: 01/01/92. to 12/31/94.

Papers on project (total): 0
Institution name: Institut "Ruđer Bošković", Zagreb (98)
Department/Institute: Department of Molecular Medicine
Address: Bijenička cesta 54
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 42 42 39

Summary: This project will be discuss a fate and a role of transferrinereceptor in the course of differentiation and maturation ofchicken erythroblast cell line (HD3 cell line). After inductionof differentiation, the level of transferin receptor increases inthe cells. Parallel with that event, transferrin receptor isreleasing from the cells in the exosome (vesicle). That phenomenis charasteristic for differentiation and maturation of mammalianred cells. The explanation of this phenomen will be help inunderstanding the mechanism of differentiation and maturation ofred blood cells. At the same time, the relation betweentransferrin receptor and hemoglobin synthesis will be discuss.With different experiments might be possible to determine theirsconection. Does synthesis of Hb depend on transferrin receptorsynthesis, or which of them is primary. The special attentionwill be give to the process responsible for the formation andrelease of exosomes. From that results we could see whether theexosome formation and its releasing is characteristic for alldifferentiating cells, or it is only a way for getting rid ofsome plasma embrane proteins.

Keywords: HD3 cell culture, transferrin receptor, differentiation, hemoglobin, nucleoside transporter

Research goals: The aim of this study was to elucidate the process of avian red cell development. During the maturation process of red cells many plasma membrane functions are diminish, nucleus and some internal structures (ribosomes and mitochondria) are extruded. From many studies was concluded that exosome formation and release is one route for remodelling the plasma membrane of imature red cells and a mechanism for the loss of specific membrane functions during maturation to the erythrocyte stage. With different approach I tryd to answer on some questions. Do chicken red cells share the property of exosome formation during maturation with mammalian cells because they retain nucleus and mitochondria? At which stage of red cell development is initiated the exosome formation? Does the process occur only at a late stage of development? In this route of ridding the cell of functions that have become obsolete or physiologically redundant associated only with penultimate stage of red cell development (i.e., reticulocyte stage)? Or, alternatively, does exosome formation occur as part of commitment to the red cell lineage and continue on to the final pre-erythrocyte stage? Second approach was to find the relationship between hemoglobin formation, increased transferrin receptor expression, and the appearance of exosomes bearing transferrin receptor.


COOPERATION - PROJECTS




COOPERATION - INSTITUTIONS


  1. Name of institution: Department of Biochemistry, Department of Neurochemistry, McGill University
    Type of institution: Economical/Production
    City: Montreal, Kanada

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Last update: 02/20/94
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