SVIBOR - Project code: 1-08-319


Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69


SVIBOR - Collecting Data on Projects in Croatia

Project code: 1-08-319


Main researcher: GRADINSKI-VRBANAC, BOJANA (14143)

Type of research: basic
Duration from: 06/01/92. to 06/01/95.

Papers on project (total): 4
Institution name: Veterinarski fakultet, Zagreb (53)
Department/Institute: Department of Physiology and Radiobiology
Address: 41001 Zagreb, Heinzelova 55
City: 41000 - Zagreb, Hrvatska
Phone: 385 (0)1 2390 172, ili 2390 111
Fax: 385 (0)1 214 697

Summary: The reduced glutathione (GSH),lipid peroxidation (TBARS) activity of glucose-6-phosphate dehydrogenase (G6PD), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), acid phosphatase (AcP), and concentrations of potassium, sodium, calcium, magnesium and inorganic phosphorus were determined in the whole blood, blood plasma and erythrocytes of fattened chickens during the mild hypothermia and posthypothermia. In the mild hypothermia there was no changes in GSH concentration of whole blood, but the significant decrease of lipid peroxides was achieved. These results indicate that the hypothermia may diminished the appearance of free radicals and thus protects the erythrocyte membranes of peroxidation (paper published in Vet. arhiv 63(3), 155-160,1993). During hypothermia and first day of posthypothermia potassium concentration was not change neighter in erythrocytes no in blood plasma, while at the same time the magnesium concentration was significantly increased. Possible explanation was done at the scientific meeting "Veterinary Science and Profession", Zagreb, 1993. The second part of this project it was planed some in vitro experiments on the erythrocyte suspension and on the cell culture of leucocytes. In the homogenates of some organs (liver, heart, kidney and muscles) the activity of enzymes mentioned above, and the concentrations of potassium, calcium and magnesium would be determined.

Keywords: Hypothermia, posthypothermia,chicken, blood, plasma, errythrocytes, leucocytes, GSH, TBARS, potassium, calcium, magnesium, G-6PD, aspartate aminotransferase, lactate dehydrogenase,acid phosphatase,inorganic phosphorus.

Research goals: Our previous resultswere shown that "mild" hypothermia provokes in the erythrocytes such changes which in some way resulted with the protection of erythrocyte membrane from the toxicity of oxigen radicals (diminution of the TBARS level) and the haemoglobine molecules from the oxidation (unchanged GSH level). At the same time increase of the magnesium concentration in blood plasma was achieved. This rise is probably due to the eflux of Mg from the liver and heart into the blood plasma, but not from the kidneys and muscles, is well known from the literature. The goal of this investigation is to determine the concentration of this ion in the erythrocytes, liver, heart, kidney and muscles, and the concentration of Ca and Mg in the blood plasma. To investigate in which extent the erythrocyte membrane structures as well as the membrane structures of some organs should be affected by the action of hypothermia, the activities of LDH, AST and GSH and TBARS concentrations in the blood plasma, liver, erythrocytes, kidney, and muscles should be determined. In experiments in vitro on the suspensions of erythrocytes and cell culture of leucocytes should be investigated the permeability of cell membranes (in mild and deep hypothermia) by measuring activity of LDH and AST in the incubation medium, and by controling the effect of some medium constituents (serum albumin) which should inchibite an eflux of Mg ion from the cells. According to the obtained results one may expect that tested parameters should be explored as aditional tests which should pointed to a degree of disturbances provoked by different steps of provoked hypothermia.

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Last update: 02/25/94