EFFECT OF HYPOTHERMIA ON ERYTHROCYTES AND CELL CULTURE OF LEUCOCYTES
Main researcher
: GRADINSKI-VRBANAC, BOJANA (14143) Assistants
ŽUPANČIĆ, ŽELJKO (55986)
Type of research: basic Duration from: 06/01/92. to 06/01/95. Papers on project (total): 4
Institution name: Veterinarski fakultet, Zagreb (53) Department/Institute: Department of Physiology and Radiobiology Address: 41001 Zagreb, Heinzelova 55 City: 41000 - Zagreb, Hrvatska
Communication
Phone: 385 (0)1 2390 172, ili 2390 111
Fax: 385 (0)1 214 697
Summary: The reduced glutathione (GSH),lipid peroxidation (TBARS)
activity of glucose-6-phosphate dehydrogenase (G6PD), aspartate
aminotransferase (AST), lactate dehydrogenase (LDH), acid phosphatase
(AcP), and concentrations of potassium, sodium, calcium, magnesium and
inorganic phosphorus were determined in the whole blood, blood plasma and
erythrocytes of fattened chickens during the mild hypothermia and
posthypothermia. In the mild hypothermia there was no changes in GSH
concentration of whole blood, but the significant decrease of lipid
peroxides was achieved. These results indicate that the hypothermia may
diminished the appearance of free radicals and thus protects the
erythrocyte membranes of peroxidation (paper published in Vet. arhiv 63(3),
155-160,1993). During hypothermia and first day of posthypothermia
potassium concentration was not change neighter in erythrocytes no in blood
plasma, while at the same time the magnesium concentration was
significantly increased. Possible explanation was done at the scientific
meeting "Veterinary Science and Profession", Zagreb, 1993. The second part
of this project it was planed some in vitro experiments on the erythrocyte
suspension and on the cell culture of leucocytes. In the homogenates of
some organs (liver, heart, kidney and muscles) the activity of enzymes
mentioned above, and the concentrations of potassium, calcium and magnesium
would be determined.
Research goals: Our previous resultswere shown that "mild"
hypothermia provokes in the erythrocytes such changes which in some way
resulted with the protection of erythrocyte membrane from the toxicity of
oxigen radicals (diminution of the TBARS level) and the haemoglobine
molecules from the oxidation (unchanged GSH level). At the same time
increase of the magnesium concentration in blood plasma was achieved. This
rise is probably due to the eflux of Mg from the liver and heart into the
blood plasma, but not from the kidneys and muscles, is well known from the
literature. The goal of this investigation is to determine the
concentration of this ion in the erythrocytes, liver, heart, kidney and
muscles, and the concentration of Ca and Mg in the blood plasma. To
investigate in which extent the erythrocyte membrane structures as well as
the membrane structures of some organs should be affected by the action of
hypothermia, the activities of LDH, AST and GSH and TBARS concentrations in
the blood plasma, liver, erythrocytes, kidney, and muscles should be
determined. In experiments in vitro on the suspensions of erythrocytes and
cell culture of leucocytes should be investigated the permeability of cell
membranes (in mild and deep hypothermia) by measuring activity of LDH and
AST in the incubation medium, and by controling the effect of some medium
constituents (serum albumin) which should inchibite an eflux of Mg ion from
the cells. According to the obtained results one may expect that
tested parameters should be explored as aditional tests which should
pointed to a degree of disturbances provoked by different steps of provoked
hypothermia. Other information about the project.