SVIBOR - Project code: 3-01-001

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
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Project code: 3-01-001


BIOCHEMICAL ASPECTS OF HUMAN SMOKING


Main researcher: KLEPAC, RATIMIR (21205)



Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/96.

Papers on project (total): 0
Institution name: Medicinski fakultet, Zagreb (108)
Communication

Summary: From 1991-1993 we have investigated biochemial modifications in human placentas and blood plasma from nonsmoker and smokergroups.We have examined level of proteins,thiocyanate and ascor- bic acid in blood plasma and homogenate of women placenta.In mitochondrial and ribosomal fraction of human placenta we have determined proteins,cytochrome P-450 and enzymes rhodanese and cytochrome c oxidase.Smoking increased thiocyanate and ascorbic acid level in women plasma and placentas.Strong smokers had hig- her activity of cytochrome P-450 than nonsmokers in placental mitochondria and ribosomes.At the same time strong smokers hada less activity of enzymes rhodanese and cytochrome c oxidase. These results show that smoking altered biochemical function of human placenta.

Keywords: Placenta,smoking,mitochondria,ribosomes,blood plasma,ascorbicacid,thiocyanate,cytochrome P-450,cytochrome c oxidase,rhodanese.

Research goals: It is known that smoking has harnful efect on human health.Smo- king is very strong risk for developmet many cancers,artherio- sclerosis and perinatal mortality.Our results show that humanplacenta is very important organ for detoxication many compounds from tobacco smoke.We have found that smoking in placenta increa- sed level of cytochrome P-450 which was a principal enzymatic system in organisam for detoxication of xenobiotics in our environment. Free oxygen radicals are important components for development many infetious and degenerative diseases.We wish to determine activity of free oxygen radicals in the patients with kidney damage and neurological insult. NADPH oxidatiom may become simple test for detection of free radicals in biological samples.

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Last update: 10/03/95
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