MORPHOLOGICAL AND STEREOLOGICAL INVESTIGATIONS OF EMBRYONIC SAC.
Main researcher
: DURST-ŽIVKOVIĆ, BOSILJKA (11035) Assistants
GRBEŠA, ĐURĐICA (94956)
Type of research: basic Duration from: 01/01/91. to 12/31/93. Papers on project (total): 20
Papers on project quoted in Current Contents: 4
Institution name: Medicinski fakultet, Zagreb (108) Department/Institute: Department of Histology and Embryology, School of Medicine, Unib Address: Šalata 3, City: 10000 - Zagreb, Croatia
Communication
Fax: 385 (0)1 4566-795
Phone: 385 (0)1 4566-946
E-mail: davor @ mamef.mef.hr
Summary: The investigations were carried out to define various
structural parameters in human placenta. The results demonstrated the
following: In human term placentas statistically significant differences
between the volume density of chorionic stems and their branches and
terminal villi (P<0.01) have been found out, what also applies to their
total volumes (P<0.02) as well as to their masses (P<0.01). Objectivitz of
intervillous space measuring has been discussed. During maturation of
placenta between the two observed periods of X lunar month (I. period from
the 38th to 39th week /12 placentas/ and II. period in the 40th week /11
placentas/) the values of macroscopic measures in the newborns and
placentas as well as quantitative parameters of parenchyma (surface density
and total surface of terminal villi, mesenchyma and blood capillaries) do
not change significantly. In syncytiotrophoblast the volume share of alpha
zone is significantly higher in the I. period, while the volume share and
the total volume of beta zones are significantly higher in the II. period
(P<0.01). The surface density of alpha zones is significantly higher
between the 38th and 39th week (P<0.01). It means that the differentiation
of placental barrier is expressed by the enlarged surface of alpha zones,
what affirms the assumption of functionally increased permeability in
transcellular transport. The arrengement of alpha and beta zones in the
placenta as a whole is even and regionaly independent. Beta zones prevail
quantitatively. Alpha zones make up only 8% of the total volume, 18% of the
total surface and 39% of the thickness of the beta zones. From the
functional point of view the obtained results support the theory that in
the last four weeks of pregnancy the growing physiological needs of fetus
correspond to the structural changes of syncytiotrophoblast. At the
beginning of this period of maturation, differentiation of trophoblast is
indicated by domination of alpha zones, the areas responsible for
transplacental gas exchange. The end of this period is characterized by the
increased value of the beta zone i.e. with a metabolically active syncytial
zone.Sexual dimorphisme in placenta is shown in alpha zone by a
significantly higher volume density (P<0.002), total volume (P<0.05) and
surface density (P<0.025) in placentas of female newborns. The
fetoplacental index is higher in male newborns. By the electron-microscopic
cytomorphological analysis of syncytiotrophoblast, beta zones were easily
identified as segments with a thicker layer of cytoplasm and numerous
nuclei, abundant cellular organelles, clusters of smooth-walled canaliculi,
vesicules and basal infoldings. On the basis of these observations we
consider that the cytomorphological differentiation of ultrastructural
elements of beta zones is easily observable and we suppose that the
quantitative analysis may be performed on electron micrographs by
stereological methods in a technically appropriate way.
Research goals: The object of this research is the embryonic sac
i.e. the architecture of placenta and embryonic membranes. The homogenity
of placental structure as a whole has been analysed and possible regional
structural differences have been stated vertically from subchorionic to
parabasal zone, and horizontally from the region of umbilical cord
insertion to the edge of the organ. Structural changes during
differentiation and maturation in chronological continuity were also
estimated and compared. The field of reference is the parenchyma of
placenta with all its structural elements: chorionic villi, its
syncytiotrophoblast with differentiated parts (alpha and beta zones),
placental barrier (membrane), mesenchymal stroma, chorionic blood vessels,
placental fibrinoids and intervillous space. Morphological stereological
light microscopic analysis include electron-microscopic level.
COOPERATION - PROJECTS
Name of project
: 3-01-181 Rječnik medicinskog (humanog i
veterinarskog) nazivlja. Name of institution: Hrvatska akademija znanosti i umjetnosti. City: 10000 - Zagreb, Croatia Other information about the project.