SVIBOR - Project code: 3-01-092

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
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Project code: 3-01-092


CONCOMITANT IMMUNITY AND ITS INFLUENCE ON TUMOUR METASTASING


Main researcher: ANDREIS, IGOR (496)



Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/94.

Papers on project (total): 4
Institution name: Medicinski fakultet, Zagreb (108)
Department/Institute: Department of physiology
Address: Šalata 3, PO Box 978
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)041-466-766

Summary: - Concomitantly immune splenocytes can adoptively transfer immunity to EAT; splenocytes from period of weakening of concomitant immunity (CI) (16th day) prevent the effects of adoptive immunotherapy. - The growth dynamics of the subcutaneously (sc) growing secondary tumour has shown no significant difference when comparred with intraperitoneally (ip) growing secondary tumour; moreover, sc growing tumour enables more precise follow-up of tumour growth. - Injection of Cyclophosphamide (Cy) (200 mg/kg) in animals which 13 or 15 days bear primary tumour (that is, 1 or 3 days before secondary tumor implantation) suppressed the secondary tumour growth; these results indicate that suppressor lymphocytes might be responsible for weakening of CI. - Neutralization test has shown that inhibitory effect of splenocytes from mice that bear primary tumour 20 days could not be abolished by Cy; in this case it seems that some other cells (possibly macrophages) are responsible for weakening of CI. - Both macrophages and lymphocytes are responsible for antitumor effect in the period of maximally expresed CI, but effect of lymphocytes is more expressed. - The results indicate the prevalence of suppressor T cells in early period of weakening of CI and of macrophages prevalence in the late period. - The number of lung metastases after iv injection of mammary carcinoma cells and of concomitant immune splenocytes in different intervals before and after tumor inoculation was the lowest when splenocytes were injected 1 or 3 days before tumor cells.

Keywords: concomitant immunity, suppressor cells, suppressor lymphocytes, suppressor macrophages, neutralization test, tumour metastasing

Research goals: The main purpose of submitted project was to investigate the factors influencing the tumor concomitant immunity (CI) dynamics, specially studying the causes and mechanisms of CI weakening or disappearance. The most part of the work was directed to the relationship between CI and tumour metastasing. It is, namely, believed that weakening or disappearance of CI enables tumour metastases to appear and grow. Because the secondarily transplanted tumour can be considered an arteficial metastasis, the investigation od CI dynamics could be useful for understanding of metastasing mechanisms. Moreover, elimination of causes of CI weakening could delay the metastases appearance. The submitted investigations have not only theoretical importance, because they could contribute to explanation of circumstances in which tumour metastases appear.

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Last update: 10/15/95
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