SVIBOR - Papers quoted in CC - project code: 3-01-110
MINISTRY OF SCIENCE AND TECHNOLOGY
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SVIBOR - Collecting Data on Projects in Croatia
Papers quoted in Current Contents on project 3-01-110
Quoted papers: 8
Other papers: 12
Total: 20
Title: Modification of protein-gliadine by phosphine PH3
- Authors:
- Biočić-Steiner, Ivka (78315)
- Bešlo, Drago
Journal: Acta Pharmaceutica
ISSN: 1330-0075
Volume: 44
Year: 1994
Pages: from 265 to 268
Language: engleski
Summary: Modification of protein-gliadin by chemical phosphyne-PH3
hasbeen investigated. Protein-gliadin as a substrate forinvestigation was
taken from wheat. Chemical phosphine-PH3 as apesticide was used for protein
protection against insecticides.Protein-gliadin contains 4 different
subunits alpha, beta, gammaand omega. The concentration of protein was
determined by theLowry method. Subunits determination of gliadine was done
by theWeber and Osborne method using SDS-electrophoresis.
Theseinvestigations detected changes in beta-subunits of the
treatedprotein-gliadin by PH3 compared to the non treated
protein.Beta-subunits apeared on electropherogram in two frations. It
iswell known that beta-subunits of gliadine contain relatively
highpercentage of disulfide cross-links. Phosphine PH3 is a
reductivereagent which results in the reduction of disulfide bond.
Twofractions of beta-subunits can be explained as structure changeson
disulfide bonds by PH3.
Keywords: protein-gliadine, phosphine PH3, pesticide, subunits, SDS-electrophoresis
Title: Effect of different diet on lung fatty acid modification
- Authors:
- Biočić-Steiner, Ivka (78315)
- Glavaš-Obrovac, Ljubica
- Martinović, Valerija
- Popović, Milivoj (38190)
Journal: Croatica Chemica Acta
Number: 1
ISSN: 0011-1643
Volume: 69
Year: 1996
Number of references: 18
Language: engleski
Summary: ÷The effect of dietary fat on the composition of lung fatty
acids was studied in Fischer rats fed two experimental semisynthetic,
isocaloric diets containing different qualities of fat (low fat diet (LFD)
and medium-chain triacylglycerols diet (MCTD)).
The effect of MCTs preparation on the lung phospho- and neutral lipids
manifests itself as a change in the degree of saturation expressed as a
ratio of saturation idex (RSi) MCTD vs CD. The saturation increased; in
neutral lipids by 70%, in phosphatidylcholine fraction by 152.6%, and in
other phospholipids by 39.5%.
The results obtained using LFD on fatty acid composition of lipids showed
the effect of desaturation expressed as a ratio of desaturation idex (RDi),
in phosphatidylcholine fraction - 1.95.
The observed effect of desaturation was a consequence of administered LFD,
which containes a lower amount of essential fatty acids, which strongly
affects the conversion of palmitic into palmitooleic acid. The LFD can be
useful as a carbohydrate diet in a special clinical situation only if
enriched with a certain amount of essential fatty acids, especially
linoleic acid.
Keywords: triacylglycerols, low fat diet, fatty acid modification, lung
Title: Medium-chain triacylglycerols effect fatty acid
modification in rat's kidney
- Authors:
- Glavaš-Obrovac, Ljubica
- Biočić-Steiner, Ivka (78315)
- Delaš, Ivančica (86340)
- Popović, Milivoj (38190)
Journal: Croatica Chemica Acta
Number: 1
ISSN: 0011-1643
Volume: 69
Year: 1996
Number of references: 30
Language: engleski
Summary: The aim of this investigation was to study the effect of a
short-time medium-chain triacylglycerols diet (MCTD) on lipids
(particularly of fatty acids) of the rat kidney. Two groups of rats
(Fischer strain) were fed during 14 days a MCTD (10.7% MCTs, 16.5 kJ/g dry
metter) or an isoenergetic control diet (standard rat chow). Total lipids
were isolated and separated into particular lipid classes by thin-layer
chromatography. No essential differences in the distibution of analyzed
lipids (cholesterol, cholesterol-ester, triacylglycerols, free fatty acids,
phosphatidylcholine, phosphatidyletanolamine and sphyngomyeline) were
noticed after a MCTD.
The results showed no changes in the concentration of total neutral lipids,
but slight changes appeared in the concentracion of the particular classes
of neutral lipids. Concentrations of cholesterol and cholesterol-esters
were decreased approximately 14% by MCTD feeding, at the same time, the
concentration of triacylglycerols, free fatty acids and esters of fatty
acid was increased by 4%. The ratio of free cholesterol to total
phospholipids (C/P) using MCTs decreased (17%) after MCTD.
The data indicate a significant increase in fatty acid saturation
(expressed as a saturation factor Sf) affected by MCTD especially in the
major fraction of kidney tissue, the sphingomyeline (385%).
Both of mentioned data (C/P ratio, Sf) and some additional one reported in
this paper, suggest that MCTD can affect the membrane fluidity, although at
the present time it is not possible to judge whether or not such effects
change the physiological function of the kidney.
Keywords: medium chain triacylglycerols, kidney, fatty acid composition, food intake
Title: Cross-linking, stabilization and detection of
glycoproteins
- Authors:
- Barbarić, Slobodan
- Česi, Vlasta
- Delaš, Ivančica (86340)
- Gebauer, Branka
- Heimgartner, U.
- Kozulić, Branko
- Mosbach, K.
Journal: Acta Pharmaceutica
ISSN: 1330-0075
Volume: 44
Year: 1994
Pages: from 353 to 365
Number of references: 38
Language: engleski
Summary: Carbohydrate residues of glycoproteins can be modified
chemically or enzimically in order to create aldehyde groups able to react
with various reagents. We have used several dihydrazides to cross-link,
intramolecularly and intermolecularly, many different glycoenzymes and
immunoglobulins. The process was employed for studying the spatial
proximity of sugar chains in glycoprotein molecules and the stability of
cross-linked glycoenzymes. The obtained results, especially those with
immunoglobulins, indicate that the method is applicable in studies of
spatial arrangement of sugar chains. It was found that some glycoenzymes
could be greatly stabilized by cross-linking their oxidized carbohydrate
chains with bifunctional cross-linkers. In an alternative procedure,
stabilization was achieved by allowing a reaction between oxidized sugar
chains and the polypeptide part of the same glycoprotein molecule. A
sensitive detection of glycoproteins bound to solid supports could be
achieved with novel polymeric hydrazides based on extremely hydrophilic
monomers. The polyhidrazides served as a bridge between a label, usually an
oxidized glycoenzyme such as alkaline phosphatase or peroxidase, and the
glacoprotein. The same polymeric hydrazides were used for detection of
antigens after their binding to oxidized sugar chains of antibodies.
Keywords: oxidized glycoproteins, hydrazide reagents
Title: Lung fatty acid manipulations in low fat diet
- Authors:
- Biočić-Steiner, Ivka (78315)
- Glavaš-Obrovac, Ljubica
- Delaš, Ivančica (86340)
- Popović, Milivoj (38190)
Journal: Acta Pharmaceutica
Number: 4
ISSN: 1330-0075
Volume: 45
Year: 1995
Number of references: 24
Language: engleski
Summary: Specific composition of fatty acids of lung
glycerophospatides, particulary phosphatidylcholine, is essential for the
physiological functions of the lung. Therefore it is important to determine
possible exogenous factors which could affect structural changes of lung
fatty acids. In this paper the effect of low fat diet (LFD) on the
composition of fatty acids of rat lung lipids is described. The LFD used
was basically composed as follows: protein 11.94%, carbohydrates 75.33% and
fat 0.66%. Control diet (CD) was isoenergetic (15 kJ/g dry substance).
During 14 days of the study, experimental animals had free access to food
and water. Upon the completion of the experiment, the animals were
sacrificed and total lipids (TL) extracted from the lungs and isolated with
chloroform-methanol. In CD and LFD, 0.54 mg TL/mg protein and 0.35 mg TG/mg
protein, respectively, were obtained. Total lipids were separated into
individual lipid classes by thin-layer chromatography. The following lipids
were isolated: triacylglycerols (TG), free fatty acids (FFA),
phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyeline
(SPH) and cerebrosides (CRB). The composition of fattty acids of particular
lung lipids was determined by gas chromatography. Results of the
qualitative and quantitative analysis of fatty acids of particular lung
lipids showed a marked increase in desaturation after LFD as compared to
control diet. This particulaly refers to C16 fatty acids: PE 143.5%, PC
95.6% and TG 66%. In C18 fatty acids, desaturation was considerably lower,
and was most pronounced in case of SPH (30.8%). The results obtained
indicated LFD to stimulate desaturation of the lung glycerophosphatide
fatty acids, which may reflect on the composition of pulmonary surfactants
and cellular membranes.
Keywords: low fat diet, lung, fatty acids, desaturation
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