SVIBOR - Papers quoted in CC - project code: 3-01-110

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Papers quoted in Current Contents on project 3-01-110


Quoted papers: 8
Other papers: 12
Total: 20


Title: Modification of protein-gliadine by phosphine PH3

Authors:
Biočić-Steiner, Ivka (78315)
Bešlo, Drago
Journal: Acta Pharmaceutica
ISSN: 1330-0075
Volume: 44
Year: 1994
Pages: from 265 to 268
Language: engleski
Summary: Modification of protein-gliadin by chemical phosphyne-PH3 hasbeen investigated. Protein-gliadin as a substrate forinvestigation was taken from wheat. Chemical phosphine-PH3 as apesticide was used for protein protection against insecticides.Protein-gliadin contains 4 different subunits alpha, beta, gammaand omega. The concentration of protein was determined by theLowry method. Subunits determination of gliadine was done by theWeber and Osborne method using SDS-electrophoresis. Theseinvestigations detected changes in beta-subunits of the treatedprotein-gliadin by PH3 compared to the non treated protein.Beta-subunits apeared on electropherogram in two frations. It iswell known that beta-subunits of gliadine contain relatively highpercentage of disulfide cross-links. Phosphine PH3 is a reductivereagent which results in the reduction of disulfide bond. Twofractions of beta-subunits can be explained as structure changeson disulfide bonds by PH3.
Keywords: protein-gliadine, phosphine PH3, pesticide, subunits, SDS-electrophoresis

Title: Effect of different diet on lung fatty acid modification

Authors:
Biočić-Steiner, Ivka (78315)
Glavaš-Obrovac, Ljubica
Martinović, Valerija
Popović, Milivoj (38190)
Journal: Croatica Chemica Acta
Number: 1
ISSN: 0011-1643
Volume: 69
Year: 1996
Number of references: 18
Language: engleski
Summary: ÷The effect of dietary fat on the composition of lung fatty acids was studied in Fischer rats fed two experimental semisynthetic, isocaloric diets containing different qualities of fat (low fat diet (LFD) and medium-chain triacylglycerols diet (MCTD)). The effect of MCTs preparation on the lung phospho- and neutral lipids manifests itself as a change in the degree of saturation expressed as a ratio of saturation idex (RSi) MCTD vs CD. The saturation increased; in neutral lipids by 70%, in phosphatidylcholine fraction by 152.6%, and in other phospholipids by 39.5%. The results obtained using LFD on fatty acid composition of lipids showed the effect of desaturation expressed as a ratio of desaturation idex (RDi), in phosphatidylcholine fraction - 1.95. The observed effect of desaturation was a consequence of administered LFD, which containes a lower amount of essential fatty acids, which strongly affects the conversion of palmitic into palmitooleic acid. The LFD can be useful as a carbohydrate diet in a special clinical situation only if enriched with a certain amount of essential fatty acids, especially linoleic acid.
Keywords: triacylglycerols, low fat diet, fatty acid modification, lung

Title: Medium-chain triacylglycerols effect fatty acid modification in rat's kidney

Authors:
Glavaš-Obrovac, Ljubica
Biočić-Steiner, Ivka (78315)
Delaš, Ivančica (86340)
Popović, Milivoj (38190)
Journal: Croatica Chemica Acta
Number: 1
ISSN: 0011-1643
Volume: 69
Year: 1996
Number of references: 30
Language: engleski
Summary: The aim of this investigation was to study the effect of a short-time medium-chain triacylglycerols diet (MCTD) on lipids (particularly of fatty acids) of the rat kidney. Two groups of rats (Fischer strain) were fed during 14 days a MCTD (10.7% MCTs, 16.5 kJ/g dry metter) or an isoenergetic control diet (standard rat chow). Total lipids were isolated and separated into particular lipid classes by thin-layer chromatography. No essential differences in the distibution of analyzed lipids (cholesterol, cholesterol-ester, triacylglycerols, free fatty acids, phosphatidylcholine, phosphatidyletanolamine and sphyngomyeline) were noticed after a MCTD. The results showed no changes in the concentration of total neutral lipids, but slight changes appeared in the concentracion of the particular classes of neutral lipids. Concentrations of cholesterol and cholesterol-esters were decreased approximately 14% by MCTD feeding, at the same time, the concentration of triacylglycerols, free fatty acids and esters of fatty acid was increased by 4%. The ratio of free cholesterol to total phospholipids (C/P) using MCTs decreased (17%) after MCTD. The data indicate a significant increase in fatty acid saturation (expressed as a saturation factor Sf) affected by MCTD especially in the major fraction of kidney tissue, the sphingomyeline (385%). Both of mentioned data (C/P ratio, Sf) and some additional one reported in this paper, suggest that MCTD can affect the membrane fluidity, although at the present time it is not possible to judge whether or not such effects change the physiological function of the kidney.
Keywords: medium chain triacylglycerols, kidney, fatty acid composition, food intake

Title: Cross-linking, stabilization and detection of glycoproteins

Authors:
Barbarić, Slobodan
Česi, Vlasta
Delaš, Ivančica (86340)
Gebauer, Branka
Heimgartner, U.
Kozulić, Branko
Mosbach, K.
Journal: Acta Pharmaceutica
ISSN: 1330-0075
Volume: 44
Year: 1994
Pages: from 353 to 365
Number of references: 38
Language: engleski
Summary: Carbohydrate residues of glycoproteins can be modified chemically or enzimically in order to create aldehyde groups able to react with various reagents. We have used several dihydrazides to cross-link, intramolecularly and intermolecularly, many different glycoenzymes and immunoglobulins. The process was employed for studying the spatial proximity of sugar chains in glycoprotein molecules and the stability of cross-linked glycoenzymes. The obtained results, especially those with immunoglobulins, indicate that the method is applicable in studies of spatial arrangement of sugar chains. It was found that some glycoenzymes could be greatly stabilized by cross-linking their oxidized carbohydrate chains with bifunctional cross-linkers. In an alternative procedure, stabilization was achieved by allowing a reaction between oxidized sugar chains and the polypeptide part of the same glycoprotein molecule. A sensitive detection of glycoproteins bound to solid supports could be achieved with novel polymeric hydrazides based on extremely hydrophilic monomers. The polyhidrazides served as a bridge between a label, usually an oxidized glycoenzyme such as alkaline phosphatase or peroxidase, and the glacoprotein. The same polymeric hydrazides were used for detection of antigens after their binding to oxidized sugar chains of antibodies.
Keywords: oxidized glycoproteins, hydrazide reagents

Title: Lung fatty acid manipulations in low fat diet

Authors:
Biočić-Steiner, Ivka (78315)
Glavaš-Obrovac, Ljubica
Delaš, Ivančica (86340)
Popović, Milivoj (38190)
Journal: Acta Pharmaceutica
Number: 4
ISSN: 1330-0075
Volume: 45
Year: 1995
Number of references: 24
Language: engleski
Summary: Specific composition of fatty acids of lung glycerophospatides, particulary phosphatidylcholine, is essential for the physiological functions of the lung. Therefore it is important to determine possible exogenous factors which could affect structural changes of lung fatty acids. In this paper the effect of low fat diet (LFD) on the composition of fatty acids of rat lung lipids is described. The LFD used was basically composed as follows: protein 11.94%, carbohydrates 75.33% and fat 0.66%. Control diet (CD) was isoenergetic (15 kJ/g dry substance). During 14 days of the study, experimental animals had free access to food and water. Upon the completion of the experiment, the animals were sacrificed and total lipids (TL) extracted from the lungs and isolated with chloroform-methanol. In CD and LFD, 0.54 mg TL/mg protein and 0.35 mg TG/mg protein, respectively, were obtained. Total lipids were separated into individual lipid classes by thin-layer chromatography. The following lipids were isolated: triacylglycerols (TG), free fatty acids (FFA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyeline (SPH) and cerebrosides (CRB). The composition of fattty acids of particular lung lipids was determined by gas chromatography. Results of the qualitative and quantitative analysis of fatty acids of particular lung lipids showed a marked increase in desaturation after LFD as compared to control diet. This particulaly refers to C16 fatty acids: PE 143.5%, PC 95.6% and TG 66%. In C18 fatty acids, desaturation was considerably lower, and was most pronounced in case of SPH (30.8%). The results obtained indicated LFD to stimulate desaturation of the lung glycerophosphatide fatty acids, which may reflect on the composition of pulmonary surfactants and cellular membranes.
Keywords: low fat diet, lung, fatty acids, desaturation


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