Investigation of the haemorrhagic fever with renal - syndrome ecology in Croatia
Main researcher
: BORČIĆ, BERISLAV (4572) Assistants
ALERAJ, BORISLAV (127040)
TURKOVIĆ, BRANKO (55593)
Type of research: applied Duration from: 01/01/91. to 12/31/95. Papers on project (total): 2
Institution name: Zavod za zaštitu zdravlja Republike Hrvatske, Zagreb (5) Department/Institute: Department of epidemiology Address: Rockefellerova 7 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 27 73 07
Fax: 385 (0) 41 27 73 07
Summary: Summary A sero-screening study of target
population segments - general and at special risk - will be carried out
with the aim to determine the incidence and the geographic
distribution of haemorrhagic fever with renal syndrome (HFRS)in
Croatia. For this purpose some 1000 sera samples of the general
population and some 500 sera samples from forest workers, both
originary from locations where HFRS was peviously registered, will be
submitted to virological testing. The indirect immunofluores cency
test will be performed (using antigen of our own produc tion).
"Positive" persons will be mapped according to their place of
residence. At the same time the fauna of potential reservoirs
of HFRS (small wild mammals), caught at selected areas in Croa tia,
will be virologically investigated as to determine the species
involved, the fequency, the dynamics adn the geograph ical
distribution of virus carriers. For the purpose some 1000-1500 small
wild mammals will be collected: a) originally it was planned to
organize 5 successive annual trappings at the Plitvice Lakes area (the
well known focus of HFRS) and b) trappings at places where human HFRS
is registered. Lungs of trapped animals will be virologically
investigated for the presence of HFRS virus by the direct
immunofluorescencu tech nique. From frozen "positive" animal lungs the
isolation of the virus on VERO cells culture will be attempted.
Results will be analysed, interpreted and published.
1
Keywords: Haemorrhagic fever with renal syndrome, Croatia, sero-screening, small wild mammals
Research goals: To determine the incidence and the
geographical distribu tion of the infection with the HFRS virus in humans
in Croatia and to determine the species of animals reservoirs of
the virus in the environment as well as their incidence and geo
graphical distribution. A sero-survey of the general population
conducted in 6 localities (ex districts): Slunj, Delnice, Ogulin,
Jastrebar sko, Velika Gorica and Požega. In all 422 sera samples were
examined. The indirect immunofluorescence test gave positive results
in 2 % of samples (Požega) up to 17 % (Jastrebarsko). Forest workers
were examined in 5 localities: Korenica, Ogu lin, Gospić, Senj and
Požega. 411 sera samples were examined with positive results ranging
from 0 % (Gospić) to 3.6 % (Požega). A special serosurvey was
conducted among two groups of first line soldiers (Novska and Ivanić
Grad). Of the 217 sera tested 10 % were positive. Small wild
mammals were trapped on 6 trapping sites: Planina Gornja (Sesvete),
Plitvička Jezera (Korenica), Kobilić (Velika Gorica), Vranovdol
(Jastrebarsko),Toti (Sesvete) and Nova Subocka (Novska). In all 724
animals of 14 different species were trapped and examined. The number
of positive animals ranked from 0 (Plani na G.) to 18.1 % (Vranovdol).
The HFRS virus was detected most frequently in the lungs of the
yellownecked mice (Apodemus flavicollis), in an average of 11.2 %,
followed by the bank vole (Cletrionomys glareolus) with 8,2 %, the
field mouse (A. agrarius) with 6,0 % and the wood mouse (A. sylvaticus)
with 4,4 %. A single positive field vole (Microtus agrestis) was also
trapped. 1 Other information about the project.