Type of research: applied Duration from: 10/05/91. to 12/31/95. Papers on project (total): 12
Papers on project quoted in Current Contents: 5
Institution name: Zavod za zaštitu zdravlja Republike Hrvatske, Zagreb (5) Department/Institute: Division of Molecular Medicine Department of Biology and Medicine RuĐer Bošković Institute Address: Bijenička cesta 54 City: 10000 - Zagreb, Croatia
Phone: 385 (01) 4561-110
Fax: 385 (01) 424-239,425-497
Summary: The prevalence of STD due to the intracellular infectious
agentslike C.trachomatis, herpes viruses, human papillomaviruses,
humanimmunodeficiency virus, is rapidly increasing for the past 30years,
while the prevalence of the bacterial STD is decreasing.These agents,
regarded as the second generation of STD, arefrequently more difficult to
identify, treat and control and,unfortunatelly, are causing serious
complicatons which result inchronic ill-health, disability and death. The
investigation ofC.trachomatis, CMV, HSV and HPV in young women and there
partnerswould determinde there incidence, there correlation with eachother
and with problems of infertility and carcinogenesis.
In the world, WHO estimates that there are some 250 million new cases of
STD each years. Among those, there are some 50 million cases of genital
chlamydia, 30 million cases of genital papillomavirus and 20 million cases
of genital herpes. There is an urgent need in all countries to establish an
adequate system for the prevention, diagnosis and treatment of STDs. From
1990 to 1993, 600 young women and partly there partners were analysed for
the presence of 3 to 4 infections agents from the second generation STD.
Most subjects had one of them. Cases of coinfections and common infections
in both partners were observed. The statistical analysis of the laboratory
results and the correlation with clinical picture was presented on several
conferences in Croatia and abroad and will be published.
Research goals: The aim of this study is to propose the best model
of control and prevention of the second generation STD. It consists in the
improvement of the method of identification of infections agents,
especially for the detection of HPV genome by the method of hybridization
and polymerase chain reaction as a preventive strategy of cervical cancer.
The main problem of the whole project was to develop a reliable and
reproducible molecular technique for HPV detection. The effort were
concentrated on the dinstinction between high risk HPVs (16, 18) from low
risk HPVs (6, 11).
COOPERATION - PROJECTS
Name of project
: 1-08-144 Ekspresija i uloga onkogena i faktora
rasta u malignim tumorima Name of institution: Institut "Ruđer Bošković", Zavod za molekularnu
medicinu City: 10000 - Zagreb, Croatia
COOPERATION - INSTITUTIONS
Name of institution
: Hrvatski Zavod za javno zdravstvo Type of institution: State institute Type of cooperation: Occasional exchange of information City: 10000 - Zagreb, Croatia Other information about the project.