SVIBOR - Project code: 3-01-141

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 3-01-141


Experimental diabetes, transplantation and immunomodulation


Main researcher: SLIJEPČEVIĆ, MILIVOJ (43700)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/93.
Papers on project (total): 49
Papers on project quoted in Current Contents: 10
Institution name: Institut "Ruđer Bošković", Zagreb (98)
Department/Institute: Department of experimetal biology and medicine Lab. LEDI
Address: Bijenička 54
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 461-111
Fax: 385 (0)41 425-497
E-mail: hadzija a olimp.irb.hr
Summary: After transplantation of 5 neonatal pancreas from syngeneic donors in 39 % of recipients the normoglycaemia was present 60 days later. Transplantation of pancreas and islets from allogeneic donors (but maid tolerant to recipient), as well as some hypoglycaemic drugs, made their metabolic condition stabile. It was shown that various relevant hematological parameters, as well as the activity of some hepatic and kidney enzymes, the amount of sialic acid and histological examinations, were improved. Transfusion of UV-irradiated whole blood provoked specific tollerance to allogeneic tissue by blocade of IL1. as well as a suppressor T-lymphocytes. Function of APC from the thymus has also been altered. Looking for etiology of leukaemia in mice we found that cell-free supernatant of ML cells provoked leukaemia and death of syngeneic recipients and reconstituted lethaly x-irradiated allogeneic recipients. We beleve that the cause of that is leucemogenic particle. UVC light povoked lyses of erithrocytes and the erythrocytes and their lysate might mitigate harmful effect of UVC light on mammalian cells. We adapted noninvasive compjuterised method for recording of somatosensory evoked potentials (SEP) after electric stimulation of healthy and diabetic mice and rats treated by drugs or after pancreas islet transplanation with the purpore to judge the grade of late complications in diabetes.

Keywords: diabetes mellitus, pancreas, islets and cells, transplantation, MHC, UV-irradiation, leukaemia, mice, rat, hypoglycaemic drugs,cytokines, cataract, evoked potentials

Research goals: We tried to formed the dependence of beginning and development of experimental diabetes and for that matter neonatal syngeneic pancreas into diabetic mice were transplanted. We expected normoglycaemia. Transplantation of syngeneic and allogeneic pancreas and isolated islets as well a hypoglycaemic drugs would made the metabolic condition stabile and haematological parameters, relevant hepatic and kidney enzymes, amount of sialic acid and histological examination will be made. We tried to escape the rejection or establish specific tolerance to allogeneic graft, diabetic mice would be pretreated with transfusion of UV-irradiated whole blood. Transplantation reaction we would like to pas by encapsulation of isolated islets in biocompatible membrane. Computerized noninvazive technique of somatosenzory electrically evoked potentials was adapted for healthy, diabetic mice and rats, before and after pancreas islet transplantation. Looking for etiology of leukaemia in mice we would like to examine if cell-free supernatant of ML non-irradiated, UV- or x-irradiated ML cells and supernatant of UV-irradiated ML cells, irradiated ML with 100 Gy of x-rays, provoke leukaemia and death of recipient.

COOPERATION - INSTITUTIONS


  1. Name of institution: KBC Rebro
    Type of institution: International organization
    City: 10000 - Zagreb, Croatia

  2. Name of institution: Farmaceutsko-biokemijski fakultet
    Type of institution: Economical/Production
    City: 10000 - Zagreb, Croatia

  3. Name of institution: Madicinski fakultet
    Type of institution: Economical/Production
    City: 10000 - Zagreb, Croatia

  4. Name of institution: Veterinarski fakultet
    Type of institution: Economical/Production
    City: 10000 - Zagreb, Croatia

  5. Name of institution: Pliva
    Type of institution: State institute
    City: 10000 - Zagreb, Croatia

  6. Name of institution: Institut za dijabetes, endokrinologiju i metaboličke bolesti "V. Vrhovec"
    Type of institution: International organization
    City: 10000 - Zagreb, Croatia

  7. Name of institution: KBC "Sestre Milosrdnice"
    Type of institution: International organization
    City: 10000 - Zagreb, Croatia

  8. Name of institution: Institut fur Angevendte Botanik, Abt. Pharmakognosie, UNI
    Type of institution: University/Faculty
    City: Hamburg, Deutchland

  9. Name of institution: Med Klinik III und Poliklinik Justus Liebig UNI
    Type of institution: International organization
    City: Giessen, Deutchland

  10. Name of institution: Dept. Med. Cell. Biol. UNI
    Type of institution: Economical/Production
    City: Uppsala, Sweden

  11. Name of institution: Banting and Best Dept. of Medical Research
    Type of institution: Economical/Production
    City: Toronto, Canada
Other information about the project.
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