SVIBOR - Project code: 3-01-200

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

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Project code: 3-01-200


THE STUDY OF IMMUNOLOGICAL MECHANISMS OF ALLERGIC DISEASES "IN VITRO"


Main researcher: ČVORIŠČEC, BRANIMIR (19815)


Assistants
Type of research: applied
Duration from: 05/30/91. to 05/30/95.
Papers on project (total): 6
Institution name: Bolnica "Sveti duh", Zagreb (129)
Department/Institute: of Pulmonary Disease and Clinical Immunology
Address: Sveti Duh 64
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 57 02 40
Fax: 385 (0)41 57 02 40
Summary: The mechanisms of allergic bronchial asthma and rhinitis, as well as specific immunotherapy are still unclear. For diagnostic procedure, specific immunotherapy, and study of the mechanisms of allergic diseases, it is necessary to use the standardized allergen extract. In collaboration with Institute of immunology, Zagreb, the Croatian national standard for Dermatophagoides pteronissinus and Ambrosia elatior, were produced. Standardised Dermatophagoides pteronissinus (Der p) allergen extrac was used for the rush immunotherapy which was performed in 40 patients with allergic rhinitis and/or bronchial asthma. The effect of sepcific immunotherapy was determined by evaluating the changes of humoral (total IgE, specific IgE, Der p specific IgG4) and cellular immunity (CD2, CD4, CD8, CD4+CD8+, CD20, CD25). During specific rush immunotherapy the change of skin tests, nonspecific and specific bronchoprovocation were also evaluated. Before and after bronchial chalenge tests by allergen Der p, the change of level of peripheral eosinophils and lymphpcyte subpopulations were measured. In supernatante of short culture of peripheral blood cells of patients with allergic bronchial asthma some mediators of allergic inflammation (histamine, triptase, PAF, TNF,ECP) after stimulation by Der p were determined. Using the model of guinea pig tracheal ring we investigated the role of neurotransmiters (tachykinins, supstance P), prostaglandins and leukotriens in allergic bronchoconstriction.

Keywords: Allergen extracts, biologic standardisation, Dermatophagoides pteronyssinus, Ambrosia elatior, bronchial asthma, rush immunotherapy, cytokine, lymphocyte subsets, bronchial challenge tests, tachykinins.

Research goals: Allergic rhinitis and bronchial asthma are common therapeutical problem. The specific immunotherapy is longlasting procedure, of unpredicitible outcome. The mechanisms of allergic bronchoconstriction and specific immunotherapy are still unclear. Therefore, the goal of this study was to: 1. production of standardized Der p and Ambrosia elatior allergen extracts, 2. evaluate specific rush immunotherapy as possible method for the treatment of allergic diseases of the respiratory system, 3. evaluate the change in humoral and cellular immunity in patients with allergic disease during specific rush immunotherapy, 4. evaluate the effect of immunotherapy on change of specific skin reactivity, and nonspecific and specific bronchial reactivity, 5. after specific bronhoprovocation investigate the role of peripheral and tissue eosinophils and lymphocyte subpopulations, 6. in culture of peripheral blood cells from allergic subjects after allergen adition, measurment of mediators (histamine, tryptase, TNF, PAF, ECP), 7. investigate the role of neurotransmiters (Tachykinins, supstance P) in allergic bronhoconstriction. We expect a significant decrease in symptoms of allergic rhinitisand bronnchial asthma in patients treated with rushimmunotherapy. Furthermore, we expect the improvement of theobjective parameters af allergic disease.

COOPERATION - INSTITUTIONS


  1. Name of institution: IMUNOLOŠKI ZAVOD, Rockefellerova 10
    Type of institution: State institute
    Type of cooperation: Systematic exchange of information
    City: 10000 - Zagreb, Croatia

  2. Name of institution: Medical College of Wisconsin, Department of Anesthesiology, Milwaukee, WI, USA
    Type of institution: University/Faculty
    Type of cooperation: Joint publishing of scientific papers
    City: 53226 - Milwaukee, USA

  3. Name of institution: Freie Universitat Berlin, Abt. fur Asthma und Klinicshe Immunologie
    Type of institution: University/Faculty
    Type of cooperation: Occasional exchange of experts
    City: 1000 - Berlin, Deutschland

  4. Name of institution: Institut "Rudjer Bošković", Biologija, Molekularna biologija, Bijenička 3
    Type of institution: State institute
    Type of cooperation: Occasional exchange of information
    City: 10000 - Zagreb, Croatia
Other information about the project.
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Last update: 10/13/95
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