SVIBOR - Project code: 3-01-211

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 3-01-211


Human Placental Growth Hormone (hGH-V)


Main researcher: MILDNER, BORIS (43404)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/95.
Papers on project (total): 0
Institution name: Bolnica "Sestre milosrdnice", Zagreb (134)
Department/Institute: Dept. of endocrinology, diabetes and metabolic diseases Laboratory of endocrinology
Address: Vinogradska c. 29
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 174-666/515
Phone: 385 (0)1 181-604
Fax: 385 (0)1 172-453
Fax: 385 (0)41 277-278
Phone: 385 (0)1 681-619
Summary: Genes for human growth hormones (hGH-N and hGH-V) and genes for placental hormones (hPL-4, hPL-3 and hPL-1) are located at a single locus on chromosome 17. The expression of growth hormone (hGH-N) in pituitary tissue is controlled by specific tissue factor Pit1/GHF-1. Other members of this hormone family, (hGH-V, hPL-4 and hPL-3), are expressed in placenta. The secretion of pituitary hGH-N hormone is regulated mainly by hypothalamic hormones but hGH-N itself as well as insulin growth factor I (IGF-I) also control the secretion. The hGH-N transcription and translation are influenced by triiodothyronine, cortisole, estradiol, IGF-I and free fatty acids. Placental growth hormone (hGH-V) is very similar to hGH-N hormone. The knowledge about the regulation of synthesis/secretion of this hormone is very limited. In human placenta the existence of a protein similar to somatostatin was reported and in rat placenta the peptide similar to somatoliberin was observed. Therefore, it is of interest to investigate the regulation of synthesis/secretion of hGH-V hormone in placenta and the influence of this hormone on the endocrine system of pregnant women. The project deals with isolation of hGH-V hormone from placenta, preparation of specific antibodies to this hormone, and the preparation of specific immunological methods for the determination of this hormone in biological fluids. By immunological techniques the synthesis/secretion of hGH-V hormone from placenta will be studied and compared with the synthesis/secretion of hGH-N hormone from the pituitary tissue.

Keywords: human growth hormone, hGH-N, hGH-V, placenta, growth hormone - receptor, growth hormone - binding proteins, prolactin, placental lactogen, RIA, monoclonal antibodies, cell culture, triiodothronine, throxine, thromboxane A2, thromboxane B2, autoanibodies

Research goals: The aim of the project is: 1. isolation and characterization of placental growth hormone (hGH-V); 2. production of monoclonal antibodies specific to pituitary hormone (hGH-N) as well as to hGH-V; 3. development of specific immunological and radioreceptor techniques to measure hGH-V in biological fluids; 4. monitoring of synthesis/secretion of hGH-V hormone and other placental hormones from placental trophoblast and syncytiotrophoblast cells in in vitro conditions, and comparison of these processes with the synthesis/secretion of pituitary hormones from pituitary tumor cells grown in in vitro primary cultures; 5. monitoring the influence of hGH-V hormone on secretion of hormones from various endocrine glands; 6. monitoring various forms of hGH-V hormone in sera of pregnant women and measuring the binding of various GH-isoforms to the growth hormone binding protein in sera. These investigations will contribute to: - knowledge about the structure and physico-chemical characteristics of hGH-V hormone; - better understanding of the mechanisms involved in the regulation of synthesis/secretion of hGH-V from placenta; - understanding of the physiological meaning of this hormone. The isolation of hGH-V hormone, production of specific antibodies and the development of specific techniques for quantitative measurement of hGH-V hormone in biological fluids is of practical interest as well, since none of these are available on the market.

COOPERATION - INSTITUTIONS


  1. Name of institution: Imunološki zavod, Zagreb
    Type of institution: State institute
    City: 10000 - Zagreb, Croatia

  2. Name of institution: Medicinski fakultet Sveučilišta u Zagrebu
    Type of institution: International organization
    City: 10000 - Zagreb, Croatia
Other information about the project.
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Last update: 12/20/95
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