Investigation of the molecular basis of metabolic diseases
Main researcher
: STAVLJENIĆ, ANA (44681) Assistants
JURIČIĆ, LJILJANA (141690)
ČVORIŠČEC, DUBRAVKA (8666)
GRAHOVAC, BLAŽENKA (112820)
TRBOJEVIĆ-ČEPE, MILICA (100580)
KUŠEC, VESNA (93843)
ŠRENGER, VESNA (124871)
SERTIĆ, JADRANKA (124856)
FUMIĆ, KSENIJA (57446)
ŠOLAJIĆ-BOŽIČEVIĆ, NEVENKA (99471)
SALZER, BRANKA (42223)
NIKŠIĆ, MARTINA (900045)
KRAJINA, ANDINA (191954)
Type of research: basic Duration from: 01/01/91. to 01/01/93. Papers on project (total): 104
Papers on project quoted in Current Contents: 20
Institution name: Medicinski fakultet, Zagreb (108) Department/Institute: Clinical institute of laboratory diagnosis, Clinical hospital center Zagreb Address: Kišpatićeva 12 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)212079
Fax: 385 (0)212079
Summary: Investigation of molecular basis of metabolic disorders was
undertaken in order to develop and test the experimental model on the
example of investiga- tion of genetic factors of atherosclerosis and
lipoprotein metabolism on similar disorders. Modern methods of molecular
biotechnology have been established and probes synthesized. The experience
and skills were presented to the participants of postcongress course
"Methods of molecular biotechnol- ogy in clinical laboratory", also in the
form of detailed instructions published in a textbook. The proven
successful model of metabolic disorders investiga- tion was applied with
certain modification in the research of dyslipoproteinamia, chronic
myeloid leukemia, HLA-C system, cystic fibrosis, ganglioside in hereditary
neuropa- thies, endemic nephropathy, MCAD gene, biotinidase and bone
metabolic diseases. The investigation included lipoprotein
characterisation in coronary heart disease, myocardial infarction,
postmenopause with hormone replacement therapy. More subjects were
included in the investigation of apoE polymorphism in chronic dementia and
Alzheimer's disease. We are collaborating in the estimation of reference
values for apoE within the multicenter project. The applied methods were
adjusted according to the ISO standards recommendations.
Research goals: investigation of molecular basis of metabolic
disorders,investigation of genetic factors of atherosclerosis, study
genetic variation of Lp(a) and apo(a) and plasminogen, establish
polyclonal and monoclonal antibodies anti Lp(a) and anti apo(a), reproduce
and determine sequence ofspecific apo(a) region in order to obtain probes
for genetic screening, investigate genetic variations of apo A, apo B and
apo E and their influence on dyslipoproteinaemias, study the role of MCM
and LCAT in lipoprotein catabolism, analyze molecular regulation of
binding kinetics of normaland structurally modified lipoproteins to
specific receptors, investigate frequency of A985G mutation in MCAD gene,
establish bcr/abl translocation in chronic myeloid leukemia, analyze
genetic basis of cystic fibrosis, study the role of ganglioside in
hereditary neuropathies, investigate etiology of endemic nephropathy,
study pathophysiology of metabolic bone diseases, analyse lipoprotein
characteristics in coronary heart disease, myocardial infarction,
postmenopause (with hormone replacement therapy), investigate apoE
polymorphism in chronic dementia and Alzheimer's disease, contribute in
the multicenter estimation of apoE reference values
COOPERATION - PROJECTS
Name of project
: > isti Name of institution: Institut fuer Medizinische Biochemie, Karl
Franzens Universitaets, Graz, Austrija City: Graz, Austrija
COOPERATION - INSTITUTIONS
Name of institution
: Centre de Medicine Preventive, Evaluation
Sante, Laboratoire de Biologie Clinique Type of institution: International organization Type of cooperation: Joint publishing of scientific papers City: Nancy, Francuska
Name of institution
: Max-Planck-Institut fuer Biologie,
Abteilung Immunogenetik Type of institution: University/Faculty Type of cooperation: Joint publishing of scientific papers City: 72076 - Tuebingen, Njemačka Other information about the project.