- Type of paper
: Paper in journal
Title:
- Authors:
- Jonjić, Nives (84573)
- Tomac, Jelena
- Peršić, Mladen
Journal: Periodicum Biologorum
Number: 1
ISSN: 0031-5362
Volume: 93
Year: 1991
Pages: from 21 to 25
Number of references: 33
Language: engleski
Summary: Immunohistological analysis of the class II antigens
expressionin the gastric mucosa was peroformed using monoclonal
antibodiesin the immunoperoxidase technique. A weak expression of the
classII antigens was observed in the lamina propria of the normalmucosa
while epithelial cells were almost negative. In contrast,the expression of
class II antigens on the epithelial cells eas aconstant finding in patients
showing the endoscopical andhistological abnormalities in gaster mucosa.
Corresponding to thelocal inflammatory cell density in the lamina propria,
a strongerneo-expression of class II antigens on epithelial cells
wasobserved. These findings provide further evidence that class IIantigens
can be induced on human stomach epithelum.
Keywords: Class II MHC antigens, epitelium, gaster
- Type of paper
: Paper in journal
Title:
- Authors:
- Jonjić, Nives (84573)
- Petr, Jilek
- Bernasconi, Sergio
- Peri, Giuseppe
- Martin-Padura, Ines
- Cenzualels, Salvatore
- Dejana, Elisabeta
- Mantovani, Alberto
Journal: The Journal of Immunology
Number: 7
ISSN: 0022-1767
Volume: 148
Year: 1992
Pages: from 2080 to 2083
Number of references: 29
Language: engleski
Summary: Our study was designed to investigate the surface
moleculesinvolved in the adhesion and cytotoxicity of activated
humanmonocytes on resting and Il-1 stimulated endothelial cells (EC).
Monocytes, exposed to the protoypic activating stimuli IFN-gammaand LPS,
showed increased binding to resting and IL-1 treated EC. Activated
monocytes were cytotoxic for resting and IL-1-treatedEC in a 24 to 48h
(H)TdR release assay. Anti-CD18 mAbsignificantly inhibited binding of
monocytes on EC: in particularthey caused 59 and 22% inhibition of adhesion
of activatedmonocytes to resting and IL-1 stimulated EC, respectively.
AntiVLA4 mAb had little or no effect on binding when used alone,
butcombined use with anti-CD18 revealed an important role for thisadhesion
pathway: in particualr, VLA4-dependent adhesionaccoounted for 40% of the
binding of activated monocytes onIL-1-treated EC. Anti-CD18 mAb caused
similar inhibition (77 and81%) of the cytotoxicity of activated monocytes
on resting andIL-1 treated EC in spite of the fact that this pathway
accountedfor only 22% of binding to activated EC. Moreover, anti-VLA4
mAb,alone or in combination with anti-CD18, had no effect oncytotoxicity.
These results suggest that adhesion of activatedmonocytes to activated EC
involves the CD18- and VLA4- dependentpathways, but that the former is
dominant for the expression ofcytotoxicity. Thus, in the ensemble of
adhesion moleculesavailable for interaction between endothelium and
activatedmonocytes, the hierarchy of their importance may vary fordifferent
functions.
- Type of paper
: Paper in journal
Title:
- Authors:
- Jonjić, Nives (84573)
- Martin-Padura, Ines
- Pollicino, Teresa
- Bernasconi, Sergio
- Petr, Jilek
- Bigotti, Aldo
- Mortarini, Roberta
- Anichini, Andrea
- Parmiani, Giorgio
- Colotta, Francesco
- Dejana, Elisabeta
- Mantovani, Alberto
- Natali, Peir Giorgio
Journal: American Journal of Pathology
Number: 6
ISSN: 1323-1330
Volume: 141
Year: 1992
Pages: from 1323 to 1330
Number of references: 25
Language: engleski
Summary: Expression of the endothelial adhesion molecule VCAM-1
wasstudied in human malignant melanoma lines by flow cytometry. Clones 2/4
and 2/14 (derived from the same lesion) hadappreciable levels of VCAM-1
expression, wheereas clone 2/21 andthe lines A2058, Mel24, and A375 were
negative. Clone 2/14 wasselected for further analysis. Exposure to tumor
necrosis factor(TNF) markeadly augmented VCAM-1 on melanoma cells.
SurfaceVCAM-1 was associated with expression of specific transcriptsthat
were augmented by TNF. Analysis by reverse transcriptase andpolymerase
chain reaction using appropriate primers revealed thatTNF-stimulated
melanoma cells expressed both 7 and 6immunoglobulin domain transcripts with
predominance of the longerspecies. Tumor necrosis factor-stimulated
melanoma cells boundmore VLA-4-expressing cells (melanoma and monocytes)
than restingtumor cells and anti-VCAM-1 monoclonal antibodies
significantlyinhibited binding, thus suggesting that surface VCAM-1
onmelanoma is functional. Analysis of melanoma tissue sectionsdemonstrated
that VCAM-1 is not a marker of transformation ofmelanocytes because it can
be detected in benign nevi. Although,unlike ICAM-1, VCAM-1 is not
correlated with tumor progression,its expression in a fraction of primary
melanomas indicates thatit may play a role in regulating host immune
response andhomotypic interactions in some malignant melanomas.
(am.J.Pathol. 1992, 141:1323-1330).
- Type of paper
: Paper in journal
Title:
- Authors:
- Jonjić, Nives (84573)
- Peri, Giuseppe
- Bernasconi, Sergio
- Sciacca, Francesca Luisa
- Colotta, Francesco
- Pelicci, Pier Giusseppe
- Lanfrancone, Luisa
- Mantovani, Alberto
Journal: J. Exp. Med.
ISSN: 1165-1174
Volume: 176
Year: 1992
Pages: from 1166 to 1174
Number of references: 43
Language: engleski
Summary: The mesothelium is a flat epithelial lining of serous
cavitiesthat could gate the traffic of molecules and cells between
thecirculation and these body compartments. The present study wasdesigned
to elucidate the capacity of mesothelial cells toexpress adhesion molecules
and chemoattractant cytokines, twofundamental mechanisms of regulation of
leukocyte recruitment. Cultured human mesothelial cells express appreciable
levels ofintercellular adhesion molecule 1 (ICAM-1) and vascular
celladhesion molecule 1 (VCAM-1), and these were increased by invitro
exposure to tumor necrosis factor (TNF), interferon gamma(IFN-gamma), or
TNF and IFN-gamma. Interleukin 1 (IL-1) was aless consistent stimulus for
adhesion molecule expression invitro. Unlike endothelial cells,used as a
reference cellpopulation, resting or stimulated mesothelial cells did
notexpress E-selectin and ICAM-2, as assessed by flow cytometry. Analysis
of VCAM-1 mRNA by reverse transcriptase and polymerasechain reaction using
appropriate primers revealed thatmesothelial cells expressed both the seven
and the six-Ig domaintranscripts, with predominance of the longer species.
Monocytesbound appreciably to "resting" and, to a greater extent,
tostimulated mesothelial cells. Monocytes exposed to IFN-gamma
andlipopolysaccharide, used as prototypic activation signals,
showedincreased capacity to bind mesothelial cells. Anti-CD18monoclonal
antibody significantly inhibited binding of monocytesto mesothelial cells,
and this blocking effect was amplified byanti-very late antigen 4.
Mesothelial cells were able to expressthe chemotactic cytokines IL-8 and
monocyte chemotactic protein 1at the mRNA and protein levels. These results
indicate thatmesothelial cells can express a set of adhesion molecules
(ICAM-1and VCAM-1) overlapping with, but distinct from, that expressedin
vascular endothelium (ICAM-1, ICAM-2, VCAM-1, E-selectin), andthat these
are functionally relevant for interacting withmononuclear phagocytes. The
regulated expression of adhesionmolecules and chemotactic cytokines by
mesothelial cells isprobably important in inflammatory and immune reactions
thatinvolve serous cavities, such as the long-known macrophageapperance and
disappearance reactions.
- Type of paper
: Paper in journal
Title:
- Authors:
- Jonjić, Nives (84573)
- Lučin, Ksenija
- Krstulja, Mira
- Iternička, Zlatko
- Mustać, Elvira
Journal: Path Res Pract
ISSN: 9799-84
Volume: 189
Year: 1993
Pages: from 979 to 984
Number of references: 39
Language: engleski
Summary: The integrins are transmembrane alfabeta heterodimers
mediatingcell-cell as well as cell-extracellular matrix interactions.
Thepresent study was designed to analyse the expression of beta-1integrins
on cryostat sections of invasive ductal carcinomas nototherwise specified
by avidin-biotin complex immunoperoxidasetechnique, and to compare it with
the morphometric prognosticindex (MPI). The results show that the
wxpression of beta-1integrins is heterogeneous in the tumors. This
heterogeneity wasobserved in quantitative and qualitative staining pattern.
Therewas an absent expression of beta-1 integrins in 22 out of 55tumors
while 33 showed staining, weak on 23 cases and strong on10 infiltrative
ductal carcinomas. Statistical analysis pointedto some correlation of
beta-1 integrins with some morphometricparameters.
Low or absent expression of beta-1 integrins correlatedsignificantly with
tumors exceeding 2 cm (p<0.0245). Moreoover, alarger proportion of tumors
with positive lymph nodes showedabsence oof beta-1 expression compared with
negative lymph node,and this was also statistically significant (p<0.0076).
Correlation between mitotic activity index and staining intensityfor beta-1
integrins was not found (p<0.372). When tumors withdifferent beta-1
expression were subdivided according to MPIvalues into two groups, one
group with a low-risk, <0.6 andsecond with a high risk, >0.6, concordance
in prognostic valuewas shown beetween MPI and beta-1 expression (p<0.0193).
Theseresults support the idea that loss of beta-1 integrins correlateswith
the invasive and metastatic potential of tumor cells.
- Type of paper
: Paper in journal
Title:
- Authors:
- Lučin, Ksenija
- Čulo, Filip
- Jonjić, Nives (84573)
Journal: Periodicum Biologorum
Number: 4
ISSN: 0031-5362
Volume: 95
Year: 1993
Pages: from 395 to 400
Number of references: 33
Language: engleski
Summary:
The aim of this study was to analyse the cells involved inimmunity to
immunogenic MCH-13 fibrosarcoma. The role of Tlymphocyte subset was
investigated by depletion of these cells byin vivo administration of
anti-CD4 and anti-CD8 monoclonalantibodies. The effect of T cell subset
depletion on the growthof primary tumor graft as well as the ability of
tumor-primedmice to reject the secondari tumor challenge was tested. In
theinitial phase of the primary tumor both T cell subsets arerequired for
the control of tumor growth. By contrast, in laterphase, only CD8+ cells
are essential. Postexcision immunity wastested as the ability of mice to
reject scondary tumor graft ofthe same tumor. Primed, nondepleted mice were
resistant to thesecondary tumor graft. Depletion of either CD4+ or CD8+
Tlymphocytes abolished the resistance. However, this waspronounoced only in
anti-CD8 treated mice, while anti-CD4treatment had only partional effect.
In atempt to characterizetumor infiltrative lymphocytes, we performed
theimmunohistological analysis of primary tumor tissue, whichconfirmed the
role of both T lympbocyte subset.
Keywords: T lymphocytes, tumor growth
- Type of paper
: M.A.
Title: The role of cellular immunity in growth-control of
methylcholantrene-induced fibrosarcoma in mice
Faculty: Medicinski fakultet Rijeka
Author: AGANOVIĆ IZET
Date of defense: 02/02/91
Language: hrvatski
Number of pages: 81
Summary: Summary
MHC-13 fibrosarcoma is an immunoogeneic tumor induced bymethylcholanthrene
in CBA mice. The growth of primary tumor isfollowed by generation of
concomitant immunity which leaves thehost with the resistance to secondary
tumor challenge. The aimof this stdy was to analyse which cells are
responsible for thisimmunity. In the initial phase of the primary tumor
growth both Tcell subsets are required for the control of the tumor
growth,while in the later phase, only CD8+ cells are required.Postexcision
immunity was tested as the ability of mice to rejecta secondary tumor graft
of the same tumor. primed, nondepletedmice were resistant to the secondary
tumor graft. Treatment withanti-CD4 and anti-CD8 monoclonal antibodies
abolished theresistance. It was obvious especially in anti-CD8 treated
mice,while nti-CD4 treatment had only partil effect. The similarresults
were obtained by the model of adoptive transfer of Tlymphocytes using
syngeneic irradiated mice as recipients.
