Immunological mechanisms in control of tumor growth
Main researcher
: JONJIĈ, NIVES (84573) Assistants
VALKOVIĈ, TONI (900590)
Type of research: basic Duration from: 01/01/92. to 01/01/96. Papers on project (total): 9
Papers on project quoted in Current Contents: 8
Institution name: Medicinski fakultet, Rijeka (62) Department/Institute: Department of patology Address: Braĉe Branchetta 20 City: 51000 - Rijeka, Croatia
Communication
Summary: In the first part of our study, the effect of T cell subset
depletion on the growth of primary tumor graft as well as the ability of
tumor-primed mice to reject the secondary tumor challenge was tested. In
the initial phase of the primary tumor growth, both T cell subsets are
required for the control of tumor growth, while in the later phase only
CD8+ cells are essential. Postexcision immunity was tested as the ability
of mice toreject the secondary tumor graft of the same tumor. Primed,
nondepleted mice were resistant to secondary tumor graft. Depletion of
either CD4+ or CD8+ T lymphocytes abolished the resistance, with more
pronounced effect in anti CD8 treated mice. In this project the
immunohistological staining was used to asses the presence of T cell
infiltrates and expression of beta 2 - microglobuline and HLA DR antigens
on tumor cells of ductal invasive carcinomas.The results were compared with
themorphometric prognostic index (MPI) that seems to be the most accurate
prognostic predictor. The extent of T cell infiltrates differed widely
between tumors, but statistically significant correlation was found only
with lymph node status. The expression of beta 2 - microglobuline and HLA
DR antigens did not correlate with any single prognostic parameter, nor
with MPI. The cellular receptor proteins mediating adhesive events are
important in tumor invasion and metastasis. In our study wetested the
expression of beta 1 - integrin molecules on the tumor cellsof
infiltrating ductal carcinoma. The results show that theexpression of this
molecules are heterogenous in the tumors. Low or absent expression of beta
1 - integrins correlated with tumors exceeding 2 cm and with positive
lymph nodes. This results support theidea that loss of beta - 1 integrins
correlates with the invasive and metastatic potential of tumor cells.