SVIBOR - Project code: 3-03-304

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 3-03-304


EXPERIMENTAL HISTOTOXICOLOGICAL INVESTIGATIONS OF FISH POISONING


Main researcher: KOZARIĆ, ZVONIMIR (95893)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/95.
Papers on project (total): 0
Institution name: Veterinarski fakultet, Zagreb (53)
Department/Institute: Department of anatomy, histology and embryology
Address: Heinzelova 55, 385 (0) 41 29 02 45
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 290 245
Phone: 385 (0)41 205 825 home
Summary: Investigation will be carried out on the carps (Cyprinus carpio L.). Experimental part of the investigations will be done in glass aquaria with dechlorinated tape water physical and chemical characteristics of which are exactly known. The effects of various toxic supstances (haevy metals, agrochemicals, industrial chemicals) on fish will be observed under acute and chronic exposure to chemicals. During each experiment a control group of fishes will be established. Histological methods will be used for examination of changes in in the organs and tissue structure after the fish is exposed to the toxic supstances. The histopathological changes of toxic supstances will be examined on various organs, particularly on the gills, liver, kidney and brain. Histochemical methods will be used for determination of enzymes' activity as well as glycogen and lipid amount in the fish organs and tissue. The investigating enzymes include the enzymes of glucose metabolism (glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, lactic acid dehydrogenase and hexokinase) and the enzymes of citric acid cycle which included succinic acid dehydrogenase, isocitric acid dehydrogenase and beta-hydroxybutyric acid dehydrogenase. In some cases, when it is important, activity of the other enzymes will be involved in the investigations. Except above-mentioned enzymes some of hydrolytic enzymes (alkaline and acid phosphatase, non-specific esterase and ATP-ase) which are involved in transport across cell membranes or in the intracellular demolishing processes will be included in investigations. Using the analytic methods the distribution of toxic supstances and their metabolites in organs and tissue will be determined. The above-mentioned methods include AAS, HPCL and ELISA.

Keywords: carps (Cyprinus carpio L.), poisoning, heavy metals, agrochemicals, tissue distribution.

Research goals: The mentioned investigations will be carried out in two ways. In the first period, the effect of toxic supstances on the structure and metabolism of carps organs will be investigated with described methods under experimental conditions with the exactly determined concentrations of toxicants. As the experiment will be carried out in the strictly experimental conditions, where all relevant parameters are known, the obtained results will point at the specific changes in the structure and metabolism of particular organs of carps. These results could contribute to understanding the mechanism of toxic action. On the other hand, the results of mentioned investigations will be improved histopathological diagnose of poisoning with particular toxic supstance. As there are few data about this in the scientific reports, the results of this part of investigation represent a significant contribution to ecotoxicology. In the second part of the investigations, which will be carried out parallely with the above-mentioned investigations, is the investigation of dissemination of the particular histopathological changes in the organs of the fish from Adriatic sea. These investigations are the part of general pathology of the marine animals. As we consider these observations to be very important as serious developmental programs for fishery as well as the increase of fish population are not possible if we are not acquinted with presen pathology and possible causes of this pathology. Every such attempt on a particular area of Adriatic sea will not give successful results without former investigations. The samples for these investigations will be taken during the fishing in particular area. Preliminary investigations have been done on the fish caught in the vicinity of Primošten and neighboring islands.
Other information about the project.
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Last update: 10/10/95
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