SVIBOR - Project code: 3-03-309


Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69


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Project code: 3-03-309

Ecological justified chemical hygienisation of liquid manure in vitro

Main researcher: ASAJ, ANTUN (972)

Type of research: applied
Duration from: 01/01/91.

Papers on project (total): 15
Institution name: Veterinarski fakultet, Zagreb (53)
Department/Institute: Department of Animal Hygiene
Address: Heizelova 55
City: 10000 - Zagreb, Croatia
Phone: 385 (0)41 290 290
Fax: 385 (0)41 214 697

Summary: 1991-1992: In water fecal emulsion of cattle, pigs, sheep and hens with 5% dry matter we achieved hygienisation of about 90% on indicator bacteria. Substrate was mixing and exposing at least for 30 minutes to the 0.05% peracetic acid with 15% active matter or exposing to the calcium hypochlorite with 45% active matter. Only for hygienisation of pigs fecal emulsion 0.10% peracetic acid was using. Chemical hygienisation of loquid manure in ecological view is justified in case of animal and human infectious disease. 1993: Ecological justified accelerated self-heating hygienisation of deep litter fattening chikens in garden composter with test bacteria put in plastic tubes we achieved hygienisation of 99.9%. Satisfactory air was present during all process of composting. The substrate was mixing every 3 days with or without adding the enzymatic-bacterial complex. During all process attention should be paid on biological, physico and chemical parameters, and on thermoisolation walls of composter. The maximum temperature in the substrate was 57 oC and 58 oC average of 45 oC for period from 14 to 42 days, and from period of 14 to 56 days of composting.

Keywords: Chemical and termical hygienisation, liquid manure, bacterial indicators, biocompost, deep litter, peracetic acid, calcium hypochlorite, enzymatic-bacterial complex.

Research goals: Hygienisation of fecal matter in ecological view of point in animal husbandry is justified in case of suspicion and manifestation of infectous disease in animal and human. We had chose two chemical disinfectant in relation on indicator bacteria. Dose and concentration was determinated for potential non-sporogenic bacteria. For practical reasons we werw starting our investignations on mixture of deep litter fattening chikens. We have also arranged International cooperation with Ministerium fur Wissenschaft und Kunst, Baden-Wurtembeg and Institut fur Tiermedizin und Tierhygiene, Universitat Hohenheim in B.R. Deutschland. German side was providing school fee for Junior Research Assistant on Project. In garden composter we have analysed possibility hygienisation of deep litter fattening chikens in relation to test bacteria, including enteropathogenic bacteria with and without enzymatic-bacterial complex.


  1. Name of project: M.z.RH533-05/92 Higijenske, tehnološke i ekološke mogućnosti kompostiranja kokošjeg gnoja, stelje, otpadaka iz valionice i zelene mase za proizvodnju humusa.
    Name of institution: Ministerium fur Wissenschaft und Kunst Baden-Wurtemberg
    City: Stuttgart, Deutschland


  1. Name of institution: Institut fur Tiermedizin und Tierhygiene Universitata Hohenheim
    Type of institution: University/Faculty
    Type of cooperation: Systematic exchange of information
    City: Stuttgart, Deutschland

  2. Name of institution: KOKA d.p.s p.o.
    Type of institution: Other
    Type of cooperation: Other
    City: 42000 - Varaždin, Croatia

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Last update: 09/29/95