Improvements in the diagnosis and control of bovine leukosis
Main researcher
: LOJKIĆ, MIRKO (26240) Assistants
ČAČ, ŽELJKO (7531)
ROIĆ, BESI (137105)
PAUKOVIĆ, ČEDOMIR (35135)
BLAŽEVIĆ, ANTE (182842)
KONČAR, LORENA (900032)
Type of research: applied Duration from: 01/01/91. to 12/15/95. Papers on project (total): 9
Institution name: Veterinarski institut, Zagreb (48) Department/Institute: Department of virology Address: Savska cesta 143 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 535-011
Fax: 385 (0)1 537-140
E-mail: mavi.veinst.hr
Summary: Program for the control and eradication of bovine enzootic
leukosis (BEL) is based on the detection of infected bovines by means of
serologica tests and culling of positive animals from further breeding
before the manifestation of clinical signs. Consequently, the methods for
serological identification of infected animals should be reliable, specific
and sensitive to enable an early detection of infected animals even with a
minimum antibody level. In Croatia, 51170 serum samples taken from bovines
wewr assessed during the last three years (1991-1993), notably 24739 in
1991, 15389 in 1992 and 11042 in 1993. Enzyme immunoassay - gp 51 ELISA was
used for the determination of antibodies to BLV in the blood serum and milk
specimens collected from cows and compared with the results of
immunodiffusion test (GDP). The results of our investigations showed that
owing to used ELISA test there was a lower incidence of "new" cases. ELISA
test showed to be 10-15% more sensitive compared to GDP test. With the use
of ELISA test it was possible to detect the antibodies in milk, which is
additional advantage over GDP test which can detect only the antibodies in
the blood serum. Thus the examination of animals is simpler, as the
withrawal of blood is avoided and this procedure usually causes a stress
situation for an animal and also makes possible the iatrogenic spreading of
infection. On the basis of obtained results it may be concluded that the
serological ELISA test is simpler and cheaper method for the diagnosis of
infected bovines. The contribution of these investigations is the use of a
new program for the diagnosis of BEL by which it is possible to meet the
international requirements and terms, in particular those in force in the
EU, for an unobstructed turnover and trade of animals.
Research goals: Bovine enzootic leukosis (BEL) is a contagious
disease caused by a virus of the family Retroviridae, genus HTLV-BLV. The
disease is often characterised by lymphocytosis of B lymphocytes. In
Croatia, about 15000 animals on big cattle-breeding farms are examined
every year. Until 1989, GDP test alone was used and in 1990, the ELISA
methodology was introduced for testing of both blood and milk samples. The
disease was diagnostified on all farms and on an average 7% of animals were
infected infected with BLV. The percentage of infected animals was reduced
to 3% in the next five years, because of the culling of positive animals
from further breeding. Immunodiffusion test, however, did not reveal all
infected animals as repeated tests detected "new" positive animals also in
the farms which have been proclaimed as BLV-free. The incidence of similar
cases has been markedly reduced since the introduction of ELISA in 1989.
The results of our investigations showed that ELISA test was by 15% more
sensitive compared to GDP test. The aim of this investigation was to
compare the results of serological blood analysis with GDP test and with
new ELISA test and to assess the correlation between test results obtained
in the blood and milk samples collected from the same animals. In addition,
we wanted to investigate the relationship between the results in a pooled
milk sample (taken from up to 50 cows), because this biotechnical method
would make the diagnosis 50 times cheaper and would also enable more rapid
identification of infected animals. Finally, the basic aim was to assess
the reability, sensitivity ans specificity of such assessment in both
individual and pooled samples in order to detect infected herds and
individual animals.