SVIBOR - Project code: 4-07-034

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 4-07-034


A BIOSYNTHESIS AND PROPERTIES OF MICROBIAL HYDROLYTIC ENZYMES


Main researcher: MARKOVIĆ, IVAN (5244)


Assistants
Type of research: applied
Duration from: 01/01/91. to 12/31/93.
Papers on project (total): 30
Papers on project quoted in Current Contents: 4
Institution name: Pliva - Istraživački institut, Zagreb (60)
Department/Institute: Biosynthesis and Biotechnology
Address: Prilaz baruna Filipovića 25
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 18-15-98
Fax: 385 (0)1 18-16-06
Fax: 385 (0)1 57-66-90
Summary: Among hydrolytic enzymes which degrade biopolymers the most studied are: amylases, proteinases and cellulases (b- glucanases) from genera Bacillus, Aspergillus etc. However, knowledge on hydrolytic enzyme biosynthesis, their isolation and stability under different conditions are still insufficient with regard to scientific interest in this field. Mechanisms of enzyme action in complex substrate degradation are also not sufficiently studied. In proposed research, the efforts will be undertaken to achieve more knowledge on biosynthesis and the properties of a-amylase, proteinase, b-glucanase from strains Bacillus subtilis, Aspergillus niger, Polyporus sp. and Clostridium histolyticum. The research will be focused on study the effect of carbon and nitrogen sources on a- amylase, proteinase and b-glucanase biosynthesis. The isolation of the enzymes from fermentation broth, their stabilization in enzyme cocktail and the physico- biochemical properties of a single enzyme, will be studied. The active components of enzyme preparation will be separated and analyzed using appropriate methods. The kinetics and mechanism of complex substrate (barley, maize) degradation will be examined. The achieved results will be marked ground for efficient use of carbohydrates, alternatively energy sources and of food raw materials.

Keywords: a-amylase, proteinase, b-glucanase, collagenase, Aspergillus niger, Bacillus suptilis, Polyporus sp., Clostridium histolyticum

Research goals: In proposed research, the efforts will be undertaken to achieve more detail and new knowledge on biosynthesis and properties of a-amylase, proteinase and b-glucanase from Bacillus subtilis, Aspergillus niger Polyporus sp. and Clostridium histolyticum. The research will comprise following issues: a) The effect of carbon and nitrogen sources on a-amylase, proteinase and b-glucanase from B. subtilis. b) Separation of the enzymes from growth and their concentrating. c) Stability of enzyme cocktails (a-amylase, proteinase, b-glucanase) and study of their physico-biochemical and kinetic characteristics. d) Separation and analysis of active components in enzyme preparations. e) Degradation of complex substrates (barley, maize) with enzyme preparations, study of reaction kinetics in heterogeneous systems, preparation of solid enzymes and their effect on weight gain in animal nutrition. The results will be presented at the scientific meetings and published in correspondent journals.
Other information about the project.
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Last update: 10/09/95
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